Abstract:
:Cells from an infinite-life-span near-diploid human fibroblast cell strain, MSU-1.1, were transformed after a single exposure to 60Co gamma radiation. The frequency of transformation as measured by the number of induced foci per 10(6) cells was a linear function of dose. Cells from 13 independent foci from gamma-irradiated cell populations and one from a nonirradiated cell population were isolated, clonally expanded and assayed for characteristics of malignantly transformed cells. Eight of the 13 focus-derived cell strains from the irradiated populations formed tumors in athymic mice with latent periods (time required for the tumors to reach 1 cm in diameter) of 4-27 weeks. Of these 8 cell strains, 3 were fully growth factor-independent, formed large colonies (> 120 microm in diameter) in 0.33% agarose at a high frequency (50%), and produced malignant tumors with a mean latency of 6 weeks or less at all sites injected. Four others formed colonies in agarose at a slightly lower frequency, were only partially growth factor-independent, and produced malignant tumors with a longer mean latency (7-18 weeks). The tumor-derived cell lines from these latter 4 cell strains, when tested for growth in agarose, showed markedly enhanced anchorage independence. The eighth tumorigenic focus-derived cell strain was growth factor-independent but could not produce large colonies in agarose. It produced benign tumors (fibromas) with a mean latency of 27 weeks. All 8 tumorigenic focus-derived cell strains had lost the transactivating function of the TP53 (formerly known as p53) gene. However, loss of TP53 activity was not sufficient to cause tumorigenicity since 3 of the 6 nontumorigenic focus-derived cell strains had also lost all TP53 transactivation function. The other 3, which included a cell strain from the unirradiated control, had wild-type TP53 alleles and did not form tumors. These latter results support the hypothesis that loss of TP53 transactivating function plays a role in focus formation, but does not directly cause tumorigenicity. This is in agreement with studies that demonstrate that the loss of TP53 transactivation facilitates the other changes required for tumorigenicity.
journal_name
Radiat Resjournal_title
Radiation researchauthors
O'Reilly S,Walicka M,Kohler SK,Dunstan R,Maher VM,McCormick JJsubject
Has Abstractpub_date
1998-11-01 00:00:00pages
577-84issue
5eissn
0033-7587issn
1938-5404journal_volume
150pub_type
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journal_title:Radiation research
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1992-03-01 00:00:00
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journal_title:Radiation research
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journal_title:Radiation research
pub_type: 杂志文章
doi:
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1986-12-01 00:00:00
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journal_title:Radiation research
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更新日期:2012-05-01 00:00:00
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journal_title:Radiation research
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更新日期:2009-02-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/RR1305.1
更新日期:2008-06-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1999-05-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/rr3319
更新日期:2005-03-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/RR2478.1
更新日期:2011-06-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1968-10-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/RR1638.1
更新日期:2009-05-01 00:00:00
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pub_type: 杂志文章
doi:10.1667/RR15566.1
更新日期:2020-07-08 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章,评审
doi:10.1667/rr2933.1
更新日期:2012-07-01 00:00:00
abstract::The effect of X irradiation on the respiration of rat thymocytes was studied. An increase in the rate of O2 uptake was observed 1 h after cells were irradiated with doses of 6-10 Gy. The radiation-induced increase in respiration could be blocked by oligomycin, an inhibitor of mitochondrial ATP synthase, suggesting con...
journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1994-04-01 00:00:00
abstract::Radiation-induced complications in bone and cartilage are of increasing concern due to potential long-term effects in cancer survivors. Healthy articular cartilage may be exposed to radiation during either chondrosarcoma treatment or in-field radiotherapy of tumors located in close proximity to articulation. Cartilage...
journal_title:Radiation research
pub_type: 杂志文章,评审
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更新日期:2015-02-01 00:00:00
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journal_title:Radiation research
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更新日期:2000-06-01 00:00:00
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journal_title:Radiation research
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更新日期:2019-12-01 00:00:00
abstract::Chomentowski, M., Kellerer, A. M. and Pierce, D. A. Radiation Dose Dependences in the Atomic Bomb Survivor Cancer Mortality Data: A Model-Free Visualization. The standard approach to obtaining nominal risk coefficients for radiation-related cancer involves fitting linear or linear-quadratic dose-response functions. Th...
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更新日期:2000-03-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1994-07-01 00:00:00
abstract::After total-body exposure to various doses of ionizing radiation, the ability of lymphocytes to interact specifically with high endothelial venules of rat cervical and mesenteric lymph nodes was analyzed in frozen sections. Following a radiation dose of 1.5 Gy, high endothelial venules remained intact and the binding ...
journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1991-02-01 00:00:00
abstract::The growth of certain UV-radiation-induced tumors is suppressed by T lymphocytes, but it has been proposed that non-T lymphocytes stimulate the growth of several of these tumors. In this study, the indirect effects of X irradiation on the growth of one such tumor in T-cell-deficient nude mice was tested. Even when the...
journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1996-12-01 00:00:00
abstract::Pulmonary carcinomas were recorded in a life-span experiment of male Sprague-Dawley rats exposed to fission neutrons. Mortality-corrected prevalences are obtained by the method of isotonic regression. In a second part of the paper a comparison is made with data obtained earlier for radon-daughter inhalations in the sa...
journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1984-06-01 00:00:00
abstract::We have tested an 836.55 MHz field with North American Digital Cellular (NADC) modulation in a 2-year animal bioassay that included fetal exposure. In offspring of pregnant Fischer 344 rats, we tested both spontaneous tumorigenicity and the incidence of induced central nervous system (CNS) tumors after a single dose o...
journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1999-09-01 00:00:00
abstract::Several studies over recent years have highlighted the possibility that radiation can induce transmissible genomic instability. Most of these involve in vitro irradiation and usually in vitro culture. Here it is reported that the short-half-life bone-seeking alpha-particle emitter (224)Ra did not induce excess transmi...
journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/0033-7587(2001)155[0345:lodtci]2.0.co;2
更新日期:2001-02-01 00:00:00
abstract::The effect of age at exposure on induction of mammary carcinoma was studied in female rats of the inbred WAG/Rij strain that were treated with estrogen. Groups of 40 animals were exposed to a single total-body dose of 1 or 2 Gy of 137Cs gamma radiation at age 8, 10, 12, 15, 22, 36 or 64 weeks. Hormone levels in the an...
journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1998-10-01 00:00:00