Abstract：
:Peripheral blood T cells were isolated from chronic progressive multiple sclerosis patients using a stepwise protocol of density gradient centrifugation, erythrocyte rosetting and adherent cell depletion, after which T cells were cultured with no added stimulus. These cultures exhibited as much as 10-fold higher 'background' proliferative activity (designated hyperactivity) than similarly prepared cultures from normal healthy control individuals. Hyperactivity was also found with T cell cultures from patients with other neurological disorders, i.e. namely, Guillain-Barré syndrome, acute stroke, myasthenia gravis or seizures. Characterizing the hyperactivity, kinetic studies showed that it was not evident until 6 days and became maximal in 8-10 day cultures; it occurred concomitantly with an increase in activated cells; and it was inhibited by anti-HLA-DR antibody, implicating the role of CD4+ T cells. Taken together, these results suggest that the hyperactivity was the result of in vitro stimulation. In further support of this view, hyperactivity was dependent on the adherence step used in the T cell isolation procedure. Although the T cell stimulus and the mechanism underlying the adherence effect is currently speculative, the hyperactivity appears to be the result of a feature common to the diseases in which it was found. The possible roles of inflammatory events in vivo and an autologous mixed lymphocyte response in vitro are discussed.
journal_name
J Neuroimmunoljournal_title
Journal of neuroimmunologyauthors
Kamin-Lewis RM,Karasanyi N,Koski CL,Lewis GKdoi
10.1016/0165-5728(94)90026-4subject
Has Abstractpub_date
1994-09-01 00:00:00pages
163-71issue
2eissn
0165-5728issn
1872-8421pii
0165-5728(94)90026-4journal_volume
53pub_type
杂志文章abstract：:We reviewed the records of 391 patients who had presented with a Clinically Isolated Syndrome and selected 205 who had performed a baseline spinal tap and MRI scan. We studied cerebrospinal fluid (CSF) and serum IgM oligoclonal bands (IgMOB) using agarose gel isoelectric focusing and analyzed the impact of baseline cl...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/j.jneuroim.2013.01.011
更新日期：2013-04-15 00:00:00
abstract：:Depending on the duration and severity, psychological tension and physical stress can enhance or suppress the immune system in both humans and animals. Although it has been established that chronic stress exerts a significant suppressive effect on immune function, the mechanisms by which affects immune responses remai...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/j.jneuroim.2017.11.005
更新日期：2018-01-15 00:00:00
abstract：:CD4+CD25+ regulatory T cells (Treg) transform the HIV-1 infected macrophage from a neurotoxic to a neuroprotective phenotype. This was demonstrated previously in a murine model of HIV-1 encephalitis induced by intracranial injection of HIV-1/vesicular stomatitis virus-infected bone marrow macrophages. In this report, ...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/j.jneuroim.2010.08.014
更新日期：2011-01-01 00:00:00
abstract：:Tissue cysts, the hallmark of chronic Toxoplasma gondii infection, are predominantly located in the brain making clearance of the parasite difficult. Currently available anti-T. gondii drugs are ineffective on cysts and fail to prevent reactivation of latent toxoplasmosis. We examined whether abrogation of inhibitory ...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/j.jneuroim.2018.03.013
更新日期：2018-06-15 00:00:00
abstract：:High mobility group box 1 (HMGB1) is a pro-inflammatory cytokine that emerges as a promising peripheral marker of inflammation. HMGB1 and C-reactive protein levels were assessed in plasma of control subjects and remitted patients with bipolar disorder (BD). HMGB1 levels were significantly higher in patients with BD as...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/j.jneuroim.2019.576993
更新日期：2019-09-15 00:00:00
abstract：:Some patients with peripheral neuropathy and gammopathy have IgM monoclonal antibodies that react with the myelin-associated glycoprotein (MAG), some 20-26 kDa glycoproteins present only in the peripheral nervous system (PNS), and some acidic glycolipids that are also PNS-specific. This communication describes an inve...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/0165-5728(86)90114-1
更新日期：1986-04-01 00:00:00
abstract：:Emergent or elective surgical procedures may be complicated by sepsis, resulting in critical illness that can lead to organ failure and death. The opioid drug, morphine is widely used to alleviate pain in post-surgical patients; however, it is well documented that chronic treatment of mice with morphine affects the pr...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/s0165-5728(98)00265-3
更新日期：1999-03-01 00:00:00
abstract：:In this study we have analysed the expression of mRNA encoding the m1-m5 mAChR subtypes in human blood mononuclear cells and subpopulations of lymphocytes using reverse transcriptase-polymerase chain reaction (RT-PCR) technique. Total RNA was extracted from human blood mononuclear cells. T cells, monocytes. EB virus t...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/0165-5728(96)00079-3
更新日期：1996-08-01 00:00:00
abstract：:The subunit composition of acetylcholine receptor-like protein(s) (AChR-LP) expressed by normal thymus was investigated. In skeletal muscle, the AChR exists in two forms, an embryonic form which contains the gamma-subunit and an adult form where the gamma-subunit is substituted by a different, homologous subunit calle...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/0165-5728(90)90150-l
更新日期：1990-09-01 00:00:00
abstract：:NFS/N mice infected with neurotropic Cas-Br-M murine leukemia virus (MuLV) at 21 days of age were resistant to neurologic disease and demonstrated MuLV-specific cytotoxic T lymphocyte (CTL) activity in spleen cells after in vitro stimulation. NFS/N mice infected with Cas-Br-M MuLV at 2 days of age failed to generate a...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/0165-5728(91)90081-h
更新日期：1991-01-01 00:00:00
abstract：:The two chemokines, monocyte chemoattractant protein (MCP)-1 and gamma-interferon inducible protein (IP)-10, are thought to be involved in the pathogenesis of multiple sclerosis (MS). We measured MCP-1 and IP-10 levels in serum and CSF samples from 38 acute and 25 stable MS patients and from 40 controls. The latter co...
journal_title：Journal of neuroimmunology
pub_type： 临床试验,杂志文章
doi：10.1016/s0165-5728(01)00261-2
更新日期：2001-04-02 00:00:00
abstract：:The inflammatory reaction in autoimmune polymyositis and rejection of transplanted myoblasts is characterized by mononuclear cell infiltration. In other settings monocytes are locally recruited by an IL-6-induced IL-8-to-MCP-1 switch. IL-6, upon binding to soluble gp80 (sIL-6R), can interact with membrane-bound ubiqui...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/j.jneuroim.2008.02.005
更新日期：2008-05-30 00:00:00
abstract：:Cardiopulmonary bypass (CPB) induces both cellular immunosuppression and an inflammatory response. In an effort to better characterize CPB-induced immune dysfunction, we examined the chemotaxic ability of human granulocytes and macrophages to D-Ala2-Met-enkephalin (DAME) and interleukin (IL)-1 alpha with computer-assi...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/0165-5728(93)90029-x
更新日期：1993-09-01 00:00:00
abstract：:NMO-IgG, a disease-specific autoantibody for neuromyelitis optica, recognizes aquaporin-4 (AQP4) and has been examined by indirect immunofluorescence assay. We developed an enzyme-linked immunosorbent assay (ELISA) to detect anti-AQP4 antibodies by establishing methods for expression in a baculovirus system and purifi...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/j.jneuroim.2008.03.009
更新日期：2008-05-30 00:00:00
abstract：:Plaque classification is proposed based on observation of 348 plaques from 52 post-mortem multiple sclerosis (MS) cases. Four plaque types, ranging from 'earliest lesion' to 'inactive', are described according to immunological activation and degree of demyelination, seen by expression of the Major Histocompatibility C...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/0165-5728(93)90251-s
更新日期：1993-07-01 00:00:00
abstract：:Rat thymocytes incubated in RPMI medium for 120-300 min release a soluble factor of molecular weight below 10,000 Da which inhibits the binding of 3H-labeled muscarinic antagonists in an uncompetitive manner, i.e. it reduces maximal specific binding (Bmax) without changing the affinity of the ligand (KD). This factor ...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/0165-5728(88)90119-1
更新日期：1988-03-01 00:00:00
abstract：:Macrophages are considered to be the predominant effector cells in multiple sclerosis (MS) and its animal model experimental autoimmune encephalomyelitis (EAE). Ultra small particles of iron oxide (USPIO) can be used to detect macrophage infiltrates in the CNS with magnetic resonance imaging (MRI). Here, we investigat...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/j.jneuroim.2007.11.008
更新日期：2008-03-01 00:00:00
abstract：:We investigated the effect of atacicept, a recombinant fusion protein blocking BLyS and APRIL and acting on B cells, on degeneration of retinal ganglion cells (RGCs) during experimental autoimmune encephalomyelitis (EAE). We used myelin oligodendrocyte glycoprotein in Brown Norway rats to induce a variant of EAE which...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/j.jneuroim.2014.01.005
更新日期：2014-03-15 00:00:00
abstract：:Autoantibodies against pituitary peptides were demonstrated in sera from multiple sclerosis (MS) patients. Ten patients with lupus erythematosus disseminatus (SLE) and 97 healthy blood donors served as controls. The sera were used as primary antibodies in the indirect immuno-enzyme cytochemical (IEC) method, with fixe...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/0165-5728(83)90007-3
更新日期：1983-10-01 00:00:00
abstract：:In the century and a half since multiple sclerosis (MS) was first recognized, the pathology of the condition has been defined with increasing detail. From the recognition and definition of MS as a clinical phenomenon, studies of the diseased brain tissue have progressed in a manner dependent on the science of the time...
journal_title：Journal of neuroimmunology
pub_type： 历史文章,杂志文章,评审
doi：10.1016/s0165-5728(99)00079-x
更新日期：1999-07-01 00:00:00
abstract：:Interleukin (IL)-10 is an anti-inflammatory cytokine that can down-regulate various aspects of the immune response. In this study we demonstrate that exposure to a psychophysiological stressor (swim stress) increases IL-10 production in female rats in response to an in vivo challenge with bacterial lipopolysaccharide ...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/j.jneuroim.2004.10.016
更新日期：2005-02-01 00:00:00
abstract：:The present study tested whether aging sensitizes hippocampal microglia to a pro-inflammatory challenge ex vivo. Hippocampal microglia from 3 and 24 mo old male F344 x BN F1 rats were exposed to LPS (0, 0.1, 1, 10 and 100 ng/ml) ex vivo. 2 h post-LPS challenge, gene expression of microglial activation markers and cyto...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/j.jneuroim.2010.05.022
更新日期：2010-09-14 00:00:00
abstract：BACKGROUND:Increased expression of monocyte chemoattractant protein type 1 (MCP-1) is associated with HIV CNS disease. This study evaluated the temporal relationships between MCP-1 expression and HIV replication in the CNS. METHODS:MCP-1 and HIV viral load (VL) were measured in serially obtained samples of plasma and ...
journal_title：Journal of neuroimmunology
pub_type： 临床试验,杂志文章
doi：10.1016/j.jneuroim.2005.07.012
更新日期：2005-12-01 00:00:00
abstract：:We investigated T cell epitopes of guinea pig myelin basic protein (MBP) that induce experimental autoimmune encephalomyelitis (EAE) in DA rats, using synthetic peptides that correspond to regions of the guinea pig MBP molecule that are homologous to rat MBP. Four peptides were encephalitogenic when tested in DA rats....
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/s0165-5728(97)00084-2
更新日期：1997-09-01 00:00:00
abstract：:IFN-gamma (IFN-gamma) has been shown to activate astrocytes to acquire immune functions. In this study the effect of IFN-gamma on murine astrocytes was investigated via microarray analysis. The activating effect of IFN-gamma on the astrocyte transcriptome showed predominance toward pathways involved in adaptive immuni...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/j.jneuroim.2006.02.015
更新日期：2006-06-01 00:00:00
abstract：:The microglia-mediated neuroinflammation plays an important role in the pathogenesis of Alzheimer's disease (AD). Advanced glycation end products (AGEs)/receptor for advanced glycation end products (RAGE) or Rho/Rho kinase (ROCK) are both involved in the development of non-specific inflammation. However, there are few...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/j.jneuroim.2017.02.010
更新日期：2017-04-15 00:00:00
abstract：:A rare case of neuromyelitis optica spectrum disorder, suspected to be bacterial meningomyelitis as the initial manifestation, is reported. The patient presented with initial symptoms of meningomyelitis and fever. Cerebrospinal fluid analysis revealed pleocytosis (1280×106/L [98% lymphocytes]) and glucose level of 1.8...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/j.jneuroim.2017.05.013
更新日期：2017-08-15 00:00:00
abstract：:This study reports on the immunocytochemical localization of tumor necrosis factor-alpha (TNF alpha) in immune-mediated demyelination and Wallerian degeneration of the rat peripheral nervous system (PNS) using teased nerve fiber preparations. In experimental autoimmune neuritis induced by active immunization (EAN) or ...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/0165-5728(93)90178-2
更新日期：1993-06-01 00:00:00
abstract：:The Lyme disease agent Borrelia garinii has been suggested to be neurotrop, preferentially affecting the nervous system. We compared the secretion of interferon-gamma in response to outer surface proteins from Borrelia garinii and Borrelia afzelii in 10 patients with neuroborreliosis. In cerebrospinal fluid, stimulati...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/s0165-5728(98)00136-2
更新日期：1998-08-14 00:00:00
abstract：:Alzheimer's disease (AD) brains contain large numbers of amyloid-beta peptide (Abeta) deposits associated with activated microglia, astrocytes and dystrophic neurites. Activated complement components and pro-inflammatory cytokines are also present, indicative of focal inflammation. However, neither Abeta, nor the chem...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/s0165-5728(00)00291-5
更新日期：2000-09-22 00:00:00
