当前位置： SCI文献检索 > JOURNAL OF NEUROIMMUNOLOGY期刊下所有文献 > Muscarinic receptor subtypes in subpopulations of human blood mononuclear cells as analyzed by RT-PCR technique.

Muscarinic receptor subtypes in subpopulations of human blood mononuclear cells as analyzed by RT-PCR technique.

Abstract：

:In this study we have analysed the expression of mRNA encoding the m1-m5 mAChR subtypes in human blood mononuclear cells and subpopulations of lymphocytes using reverse transcriptase-polymerase chain reaction (RT-PCR) technique. Total RNA was extracted from human blood mononuclear cells. T cells, monocytes. EB virus transformed B cells and from two leukemic cell lines and analysed by RT-PCR. Our results indicate that mRNAs for the m3, m4 and m5 muscarinic subtypes are expressed in mononuclear cells and purified T cells while m1 and m2 mRNAs were not detected in these cells. No m1-m5 subtype mRNA was detected in B cells and monocytes, indicating absence of muscarinic receptors in these cells. The expression of muscarinic subtypes in the leukemic T cell line. Peer, and the promyelocytic leukemic cell line HL-60 was different from peripheral mononuclear cells. Both m3 and m5 subtypes were expressed in Peer cells but not the m4 subtype, whereas the m4 and m5 subtypes detected in HL-60 cells.

journal_name

J Neuroimmunol

journal_title

Journal of neuroimmunology

authors

Hellström-Lindahl E,Nordberg A

doi

10.1016/0165-5728(96)00079-3

subject

Has Abstract

pub_date

1996-08-01 00:00:00

pages

139-44

issue

1-2

eissn

0165-5728

issn

1872-8421

pii

0165-5728(96)00079-3

journal_volume

68

pub_type

杂志文章

相关文献

JOURNAL OF NEUROIMMUNOLOGY文献大全
  • T lymphocyte subset abnormalities in peripheral blood from patients with the Guillain-Barré syndrome.

    abstract：:T lymphocytes are probably of pathogenic importance in many autoimmune diseases. Recently, deviations of circulating T-helper (CD4+) subpopulations have been noticed. Blood samples from 12 patients with Guillain-Barré syndrome (GBS) were studied with flow cytometry during their disease course to define circulating T c...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章

    doi：10.1016/0165-5728(94)90032-9

    authors： Dahle C,Vrethem M,Ernerudh J

    更新日期：1994-09-01 00:00:00

  • A role for reactive oxygen species in endotoxin-induced elevation of MOR expression in the nervous and immune systems.

    abstract：:This study examined the mechanism by which exposure to lipopolysaccharide (LPS) alters mu-opioid receptor (MOR) expression in immune and neuronal cells using an in vitro conditioned medium model system. We found that LPS stimulated the intracellular accumulation of reactive oxygen species (ROS) and MOR expression in m...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章

    doi：10.1016/j.jneuroim.2011.05.009

    authors： Langsdorf EF,Mao X,Chang SL

    更新日期：2011-07-01 00:00:00

  • Beta-adrenoceptor mediated surgery-induced production of pro-inflammatory cytokines in rat microglia cells.

    abstract：:Immunological changes initiated by major operative injury may result in inflammatory responses in both peripheral and central nervous system, which may lead to organ dysfunction. Recent studies indicate that beta-adrenergic receptors (beta-ARs) may mediate production of pro-inflammatory cytokines in the brain. In the ...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章

    doi：10.1016/j.jneuroim.2010.04.006

    authors： Wang J,Li J,Sheng X,Zhao H,Cao XD,Wang YQ,Wu GC

    更新日期：2010-06-01 00:00:00

  • B cell follicle-like structures in multiple sclerosis-with focus on the role of B cell activating factor.

    abstract：:B lymphocytes play an important role in the pathogenesis of multiple sclerosis (MS). Follicle-like structures (FLS) have recently been found in the subarachnoid space in the leptomeninges in some patients with secondary progressive MS (SPMS). They contain proliferating B lymphocytes, plasma cells, helper T lymphocytes...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章,评审

    doi：10.1016/j.jneuroim.2014.05.010

    authors： Haugen M,Frederiksen JL,Degn M

    更新日期：2014-08-15 00:00:00

  • Elevated B-cell activating factor BAFF, but not APRIL, correlates with CSF cerebellar autoantibodies in pediatric opsoclonus-myoclonus syndrome.

    abstract：:Childhood opsoclonus-myoclonus syndrome (OMS) occurs idiopathic or, in association with a neuroblastoma, as a paraneoplastic syndrome. Since autoantibodies were identified in some patients, an autoimmune pathogenesis has been suspected. While the newly discovered B-cell activating factors BAFF and APRIL are involved i...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章

    doi：10.1016/j.jneuroim.2009.03.006

    authors： Fühlhuber V,Bick S,Kirsten A,Hahn A,Gerriets T,Tschernatsch M,Kaps M,Preissner KT,Blaes F,Altenkämper S

    更新日期：2009-05-29 00:00:00

  • Occurrence and clinical relevance of an interleukin-4 gene polymorphism in patients with multiple sclerosis.

    abstract：:An epistatic gene interaction has been advocated to explain disease susceptibility in multiple sclerosis (MS). Cytokine genes are possible candidates due to the central role played by cytokines in the regulation of the immune-mediated pathogenetic process leading to central nervous system demyelination in these patien...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章

    doi：10.1016/s0165-5728(97)00058-1

    authors： Vandenbroeck K,Martino G,Marrosu M,Consiglio A,Zaffaroni M,Vaccargiu S,Franciotta D,Ruggeri M,Comi G,Grimaldi LM

    更新日期：1997-06-01 00:00:00

  • Cerebrospinal fluid oligoclonal IgM bands predict early conversion to clinically definite multiple sclerosis in patients with clinically isolated syndrome.

    abstract：:We reviewed the records of 391 patients who had presented with a Clinically Isolated Syndrome and selected 205 who had performed a baseline spinal tap and MRI scan. We studied cerebrospinal fluid (CSF) and serum IgM oligoclonal bands (IgMOB) using agarose gel isoelectric focusing and analyzed the impact of baseline cl...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章

    doi：10.1016/j.jneuroim.2013.01.011

    authors： Ferraro D,Simone AM,Bedin R,Galli V,Vitetta F,Federzoni L,D'Amico R,Merelli E,Nichelli PF,Sola P

    更新日期：2013-04-15 00:00:00

  • EPA protects against muscle damage in the mdx mouse model of Duchenne muscular dystrophy by promoting a shift from the M1 to M2 macrophage phenotype.

    abstract：:In dystrophic mdx mice and in Duchenne muscular dystrophy, inflammation contributes to myonecrosis. Previously, we demonstrated that eicosapentaenoic acid (EPA) decreased inflammation and necrosis in dystrophic muscle. In the present study, we examined the effects of EPA and the corticoid deflazacort (DFZ) as modulato...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章

    doi：10.1016/j.jneuroim.2013.09.007

    authors： Carvalho SC,Apolinário LM,Matheus SM,Santo Neto H,Marques MJ

    更新日期：2013-11-15 00:00:00

  • Serum CD95 of relapsing remitting multiple sclerosis patients protects from CD95-mediated apoptosis.

    abstract：:Failure of CD95-mediated apoptosis as a potential negative regulatory mechanism of T cell expansion may be involved in T cell-mediated autoimmune diseases such as multiple sclerosis (MS). Recently, soluble CD95 has been shown to be elevated in MS patients with active disease. Here, we report that the sera of MS patien...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章

    doi：10.1016/s0165-5728(98)00032-0

    authors： Zipp F,Otzelberger K,Dichgans J,Martin R,Weller M

    更新日期：1998-06-15 00:00:00

  • Blunted stress responses in delayed type hypersensitivity in mice lacking the neuronal isoform of nitric oxide synthase.

    abstract：:Nitric oxide (NO) is implicated in inflammation and hypothalamic-pituitary responses to immune stimuli; however, the specific role of NO from neurons during stress-induced immune responses remains unspecified. We measured antigen-specific delayed-type-hypersensitivity (DTH) responses in the skin of wild-type (WT) and ...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章

    doi：10.1016/s0165-5728(03)00175-9

    authors： Bilbo SD,Hotchkiss AK,Chiavegatto S,Nelson RJ

    更新日期：2003-07-01 00:00:00

  • Role of the Programmed Death-1 (PD-1) pathway in regulation of Theiler's murine encephalomyelitis virus-induced demyelinating disease.

    abstract：:Programmed death-1 (PD-1) belongs to the CD28 family of co-stimulatory and co-inhibitory molecules and regulates adaptive immunity. This molecule induces the development of regulatory T cells, T cell tolerance, or apoptosis. We examined the role of PD-1 pathway in Theiler's murine encephalomyelitis virus (TMEV)-induce...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章

    doi：10.1016/j.jneuroim.2014.06.018

    authors： Takizawa S,Kaneyama T,Tsugane S,Takeichi N,Yanagisawa S,Ichikawa M,Yagita H,Kim BS,Koh CS

    更新日期：2014-09-15 00:00:00

  • Thymic myoid cells express high levels of muscle genes.

    abstract：:To explore the possible contribution of thymic myoid cells in tolerance induction mechanisms, we quantified by real-time RT-PCR, the expression of 12 muscle genes (the five subunits of acetylcholine receptor, Musk, rapsyn, utrophin, ErbB2, ErbB3, troponin T, and MCK) in a human thymic myoid cell line (MITC), compared ...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章

    doi：10.1016/j.jneuroim.2003.11.013

    authors： Mesnard-Rouiller L,Bismuth J,Wakkach A,Poëa-Guyon S,Berrih-Aknin S

    更新日期：2004-03-01 00:00:00

  • Phenotypes and alloantigen-presenting activity of individual clones of microglia derived from the mouse brain.

    abstract：:To clarify the origin and function of the microglia residing in the central nervous system, we cloned brain cells from newborn and adult mice in soft agar containing the macrophage-specific growth factor, colony-stimulating factor-1 and expanded the cells from individual colonies in liquid culture medium. The results ...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章

    doi：10.1016/0165-5728(92)90071-r

    authors： Moore SC,McCormack JM,Armendariz E,Gatewood J,Walker WS

    更新日期：1992-12-01 00:00:00

  • Expression and function of inducible nitric oxide synthase in neurons.

    abstract：:Enzymatically derived nitric oxide (NO) has been implicated in numerous physiological and pathological processes in the brain. Whereas during development NO participates in developmental and maturation processes, excess NO production in the adult in response to inflammation, injury, or trauma participates in both cell...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章,评审

    doi：10.1016/s0165-5728(01)00246-6

    authors： Heneka MT,Feinstein DL

    更新日期：2001-03-01 00:00:00

  • MicroRNAs associated with the pathogenesis of multiple sclerosis.

    abstract：:Multiple sclerosis (MS) is not only an autoimmune disease in which autoreactive immune cells against myelin damage axons and nerves in the central nervous system, but also a neurodegenerative disease, in which progressive loss of structure and function of neurons occurs. The mechanisms of MS pathogenesis have not been...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章,评审

    doi：10.1016/j.jneuroim.2016.04.014

    authors： Huang Q,Xiao B,Ma X,Qu M,Li Y,Nagarkatti P,Nagarkatti M,Zhou J

    更新日期：2016-06-15 00:00:00

  • Local immune responses in the rat cerebral cortex after middle cerebral artery occlusion.

    abstract：:This study describes local immune responses in cerebral ischemia induced by permanent occlusion of the middle cerebral artery (MCAO) in the rat. The temporal and spatial pattern of leukocyte infiltration was characterized immunocytochemically using monoclonal antibodies against CD5, a pan T cell marker, against CD4 an...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章

    doi：10.1016/0165-5728(94)90010-8

    authors： Schroeter M,Jander S,Witte OW,Stoll G

    更新日期：1994-12-01 00:00:00

  • Low-dosage of rituximab in Chinese patients with neuromyelitis optica spectrum disorder.

    abstract：:Rituximab has been effectively used for treating neuromyelitis optica spectrum disorder (NMOSD) for several years. However those regimens exert a heavy burden on Chinese patients. The aim of our study was to investigate an effectiveness, economic alternatives of RTX. The enrolled patients received different immunosupp...

    journal_title：Journal of neuroimmunology

    pub_type： 临床试验,杂志文章

    doi：10.1016/j.jneuroim.2018.02.004

    authors： Lin J,Li X,Xue B,Tong Q,Chen Z,Zhu W,Li J,Xia J

    更新日期：2018-04-15 00:00:00

  • Developmental expression of cell type-specific markers in mouse cerebellar cells in vitro.

    abstract：:The expression of several cell type-specific markers was studied by indirect immunofluorescence in discontinuous BSA gradient fractionated and unfractionated mouse cerebellar cells cultured for 3 days in vitro from embryonic day 13 through postnatal day 9. Cell surface antigen NS-4 and tetanus toxin receptors are pres...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章

    doi：10.1016/0165-5728(81)90024-2

    authors： Schnitzer J,Schachner M

    更新日期：1981-12-01 00:00:00

  • A novel calpain inhibitor for the treatment of acute experimental autoimmune encephalomyelitis.

    abstract：:Aberrant activation of calpain plays a key role in the pathophysiology of several neurodegenerative disorders. Calpain is increasingly expressed in inflammatory cells in EAE and is significantly elevated in the white matter of patients with multiple sclerosis, thus calpain inhibition could be a target for therapeutic ...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章

    doi：10.1016/j.jneuroim.2006.08.005

    authors： Hassen GW,Feliberti J,Kesner L,Stracher A,Mokhtarian F

    更新日期：2006-11-01 00:00:00

  • Delta 9-tetrahydrocannabinol: a novel treatment for experimental autoimmune encephalomyelitis.

    abstract：:Since multiple sclerosis (MS) is believed to be an immune-mediated disease, it follows that its therapies should be directed towards modulating the immune system. Current MS treatments, which include the use of exogenous steroids that are immunosuppressive, do not meet therapeutic objectives. delta 9-Tetrahydrocannabi...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章

    doi：10.1016/0165-5728(89)90075-1

    authors： Lyman WD,Sonett JR,Brosnan CF,Elkin R,Bornstein MB

    更新日期：1989-06-01 00:00:00

  • Spatio-temporal differences in the profile of murine brain expression of proinflammatory cytokines and indoleamine 2,3-dioxygenase in response to peripheral lipopolysaccharide administration.

    abstract：:The mechanisms underlying in vivo activation of indoleamine 2,3-dioxygenase (IDO), a tryptophan-catabolizing enzyme that mediates in the brain the induction of depressive-like behavior by peripheral innate immune system stimulation are still poorly understood. By monitoring how cytokines parallel IDO mRNA expression i...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章

    doi：10.1016/j.jneuroim.2008.06.011

    authors： André C,O'Connor JC,Kelley KW,Lestage J,Dantzer R,Castanon N

    更新日期：2008-08-30 00:00:00

  • Recognition of neurokinin 1 receptor (NK1-R): an antibody to a peptide sequence from the third extracellular region binds to brain NK1-R.

    abstract：:Substance P (SP) can produce cytokine-like responses by astrocytes and mononuclear cells. In an effort to identify neurokinin-1-receptors (NK1-R), an antibody to NK1-R was generated by using a linear peptide sequence from the deduced third extracellular region (ECR) corresponding to the seven transmembrane rat brain N...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章

    doi：10.1016/s0165-5728(96)00033-1

    authors： van Ginkel FW,Pascual DW

    更新日期：1996-06-01 00:00:00

  • Adult thymus expresses an embryonic nicotinic acetylcholine receptor-like protein.

    abstract：:The subunit composition of acetylcholine receptor-like protein(s) (AChR-LP) expressed by normal thymus was investigated. In skeletal muscle, the AChR exists in two forms, an embryonic form which contains the gamma-subunit and an adult form where the gamma-subunit is substituted by a different, homologous subunit calle...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章

    doi：10.1016/0165-5728(90)90150-l

    authors： Nelson S,Conti-Tronconi BM

    更新日期：1990-09-01 00:00:00

  • Matrix metalloproteinase-12 is expressed in phagocytotic macrophages in active multiple sclerosis lesions.

    abstract：:Matrix metalloproteinases (MMPs) are proteases involved in extracellular matrix (ECM) remodeling, leukocyte infiltration into lesions and myelin degradation in the central nervous system (CNS) disease multiple sclerosis (MS). We have investigated whether MMP-12 (macrophage metalloelastase) is expressed in MS lesions a...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章

    doi：10.1016/s0165-5728(03)00036-5

    authors： Vos CM,van Haastert ES,de Groot CJ,van der Valk P,de Vries HE

    更新日期：2003-05-01 00:00:00

  • Cross-reactive anti-galactocerebroside antibodies and Mycoplasma pneumoniae infections in Guillain-Barré syndrome.

    abstract：:Anti-galactocerebroside (GalC) antibodies are reported to be present in GBS patients with preceding Mycoplasma pneumoniae (MP) infection. We investigated the presence of anti-GalC reactivity in serum of a large group of GBS patients using ELISA and compared this with healthy controls and individuals with an uncomplica...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章

    doi：10.1016/s0165-5728(02)00209-6

    authors： Ang CW,Tio-Gillen AP,Groen J,Herbrink P,Jacobs BC,Van Koningsveld R,Osterhaus AD,Van der Meché FG,van Doorn PA

    更新日期：2002-09-01 00:00:00

  • ELI-spot of Th-1 cytokine secreting PBMC's in multiple sclerosis: correlation with MRI lesions.

    abstract：:The Th1-like cytokines, interleukin 2 (IL-2), interferon gamma (IFN-gamma), and lymphotoxin alpha (LT-alpha) have been implicated in the immunopathogenesis of multiple sclerosis (MS) and experimental allergic encephalomyelitis (EAE), an animal model of immune mediated demyelination. These cytokines have been associate...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章

    doi：10.1016/s0165-5728(98)00008-3

    authors： Calabresi PA,Fields NS,Farnon EC,Frank JA,Bash CN,Kawanashi T,Maloni H,Jacobson S,McFarland HF

    更新日期：1998-05-15 00:00:00

  • Immunocytochemical characterisation of cell cultures grown from dissociated 1-2-day post-natal rat cerebral tissue. A developmental study.

    abstract：:A range of cell-specific markers have been employed with immunocytochemical methods to characterise and quantitate the cell types present in mixed brain cell cultures derived from dissociated 1-2-day post-natal rat cerebral hemispheres and grown in the presence of FCS. Protoplasmic astrocytes (GFAP+, A2B5-) were the m...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章

    doi：10.1016/s0165-5728(84)80002-8

    authors： Walker AG,Chapman J,Bruce CB,Rumsby MG

    更新日期：1984-11-01 00:00:00

  • An Epstein Barr virus-related cross reactive autoimmune response in multiple sclerosis in Norway.

    abstract：:In studies of patients in Norway with multiple sclerosis (MS), we have found cross reactive autoantibodies related to the Epstein Barr virus nuclear antigen-1 (EBNA-1). The MS patients had elevated IgG antibody to EBNA-1, as measured by reactivity with a synthetic glycine/alanine peptide, P62, which represents the gly...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章

    doi：10.1016/0165-5728(96)00069-0

    authors： Vaughan JH,Riise T,Rhodes GH,Nguyen MD,Barrett-Connor E,Nyland H

    更新日期：1996-09-01 00:00:00

  • Variable patterns of anti-GM(1) IgM-antibody populations defined by affinity and fine specificity in patients with motor syndromes: evidence for their random origin.

    abstract：:Elevated titers of serum antibodies against GM(1)-ganglioside are associated with a variety of autoimmune neuropathies. Although much evidence indicates that these autoantibodies play a primary role in the disease processes, the mechanism of their appearance is unclear. Low-affinity anti-GM(1) antibodies of the IgM is...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章

    doi：10.1016/s0165-5728(01)00355-1

    authors： Lopez PH,Lardone RD,Irazoqui FJ,Villa AM,Di Egidio M,Saizar RD,Sica RE,Nores GA

    更新日期：2001-09-03 00:00:00

  • Depressive state and chronic fatigue in multiple sclerosis and neuromyelitis optica.

    abstract：:Depression and chronic fatigue are frequently present in multiple sclerosis (MS); however, the prevalence rates have not been investigated in neuromyelitis optica (NMO). Thirty-nine consecutive NMO and 75 MS patients were compared using self-rating questionnaires for depressive states, daily activity, and fatigue, as ...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章

    doi：10.1016/j.jneuroim.2015.05.007

    authors： Akaishi T,Nakashima I,Misu T,Fujihara K,Aoki M

    更新日期：2015-06-15 00:00:00