Cell density and exogenous CNTF affect CNTF mRNA levels in glial cell cultures.

Abstract:

:Regulation of CNTF mRNA was investigated in primary cortical astrocytes cultured from newborn rats and in C6 glioma cells. Northern blot analysis indicated that semi-confluent astrocyte cell cultures. CNTF added to confluent astrocyte cultures down-regulated CNTF message in a dose-dependent fashion. In contrast, when CNTF was given to semi-confluent astrocyte cultures the level of CNTF mRNA was up-regulated. C6 glioma cells also showed a cell density-dependent expression of CNTF: cells from confluent cultures expressed detectable amounts of CNTF mRNA whereas those from semi-confluent cultures did not. Thus, CNTF mRNA expression by astroglial and glioma cells is regulated by cell contact and exogenous CNTF in vitro.

journal_name

Neuroreport

journal_title

Neuroreport

authors

Meyer V,Unsicker K

doi

10.1097/00001756-199410000-00026

subject

Has Abstract

pub_date

1994-10-03 00:00:00

pages

1946-8

issue

15

eissn

0959-4965

issn

1473-558X

journal_volume

5

pub_type

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