pH transients due to monosynaptic activation of GABAA receptors in rat hippocampal slices.

Abstract:

:Extracellular pH transients were evoked in rat hippocampal brain slices by activation of a monosynaptic inhibitory pathway following pharmacological blockade of glutaminergic transmission. Repetitive stimulation in stratum radiatum near the recording site in stratum pyramidale evoked an immediate alkaline shift which was potentiated by pentobarbital and blocked by picrotoxin but not by 2-hydroxy-saclofen. Benzolamide, a poorly permeant inhibitor of carbonic anhydrase (CA), and prontosil-dextran 5000, a macromolecular CA inhibitor, abolished the alkaline transients evoked by stimulation and by exogenous GABA. Thus an extracellular CA is involved in regulating interstitial pH in brain, and the stimulation-induced alkaline transients are caused by net influx of CO2 into CA1 neurons in response to efflux of bicarbonate across postsynaptic GABAA receptor channels.

journal_name

Neuroreport

journal_title

Neuroreport

authors

Kaila K,Paalasmaa P,Taira T,Voipio J

doi

10.1097/00001756-199201000-00028

subject

Has Abstract

pub_date

1992-01-01 00:00:00

pages

105-8

issue

1

eissn

0959-4965

issn

1473-558X

journal_volume

3

pub_type

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