Abstract：
:Regulation of tyrosine hydroxylase (TH) by second messenger pathway activators was examined in rat olfactory bulb cell cultures. The number of TH-immunoreactive neurons was increased 2-3-fold by 36 h treatments with forskolin (Fsk, 10(-6) M) or phorbol myristate acetate (PMA, 10(-7) M), but was not significantly increased by a depolarizing concentration of KCl (45 mM). In contrast, KCl increased media [Met5]enkephalin (ME) immunoreactivity 2-fold in these cultures, equivalent to stimulation with Fsk or PMA. The possibility was examined that ME or another opioid produced by the cultures selectively inhibited the TH response to KCl. Pretreatment with the opioid receptor antagonist naloxone (10(-6) M) greatly increased the number of TH-immunoreactive neurons observed in response to KCl treatment, but had no effect on basal or Fsk-stimulated TH immunostaining, nor on basal or stimulated ME release. The increase in TH-immunoreactivity observed with combined KCl plus naloxone treatment was prevented by pretreating the cultures with the calcium channel blocker nimodipine (10(-6) M), which had no effect on Fsk stimulation or basal TH immunostaining. These data suggest that endogenous opioids selectively inhibit KCl-stimulated Ca2+ entry and thus TH induction in olfactory bulb cell cultures. These cultures offer a simple model system for further study of TH regulation in dopaminergic neurons.
journal_name
Brain Resjournal_title
Brain researchauthors
McMillian MK,Mullis SB,Wu GC,Hudson PM,Pennypacker KR,Hong JSdoi
10.1016/s0006-8993(09)90015-4subject
Has Abstractpub_date
1994-09-26 00:00:00pages
105-11issue
1-2eissn
0006-8993issn
1872-6240pii
S0006-8993(09)90015-4journal_volume
658pub_type
杂志文章相关文献
BRAIN RESEARCH文献大全abstract：:Intracranial microdialysis was used to measure dopamine (DA) release in the ventrolateral neostriatum of freely moving rats before and after unilateral tactile stimulation was applied to the orofacial region. Several behavioral parameters which have been linked to changes in nigrostriatal DA transmission (scanning, or...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(91)90842-j
更新日期：1991-07-12 00:00:00
abstract：:The effects of intracerebral injections of brain-gut peptides in the preoptic region on the secretion of luteinizing hormone (LH) and prolactin (PRL) were examined in ovariectomized, estrogen-primed rats. Gastrin or cholecystokinin-octapeptide injection in the preoptic region induced a moderate increase of circadian r...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(87)90330-1
更新日期：1987-05-05 00:00:00
abstract：:We examined the effect of gangliosides on Schwann cell cultures isolated from neonatal rat sciatic nerves. Addition of gangliosides (GM1, GM3, and ganglioside mixture) at concentrations between 0.25 and 2 mg/ml significantly diminished both the baseline rate of proliferation of the Schwann cells and their response to ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(88)90442-8
更新日期：1988-12-06 00:00:00
abstract：:Melatonin is synthesized rhythmically under control of circadian oscillators by the retinas of non-mammalian vertebrates. Here we report that the retinas of some strains of laboratory mice exhibit robust circadian rhythms of melatonin synthesis which can be entrained by light in vitro. The rd mutation results in progr...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(97)01446-7
更新日期：1998-04-13 00:00:00
abstract：:Six cats were trained to eat while partially restrained and while thermal pulse stimuli (43-60 degrees C, 5 s duration) were delivered to the upper hindlimbs. Food and stimulus delivery were under programmed electronic control. The probability and latency of 3 natural, unlearned nocifensive behaviors were electronical...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(89)91036-6
更新日期：1989-10-30 00:00:00
abstract：:This EEG study (N=33) examined event-related potentials associated with conflict between activated responses in the Stroop task, in order to examine the conflict monitoring theory of cognitive control, which predicts the strength of exerted control to be proportional to the detected level of conflict. However, existin...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2016.08.041
更新日期：2016-11-01 00:00:00
abstract：:The effects of glucagon injections (25, 100 and 200 ng) into the paraventricular nucleus of the hypothalamus (PVN) were investigated in an open-circuit calorimeter. Wistar rats were tested, with no food available during the tests. High doses of glucagon (100 and 200 ng) produced small and short-lasting increases in en...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(93)90663-8
更新日期：1993-12-10 00:00:00
abstract：:The ganglion cells are the output neurons of the retina. There is, however, relatively little known about the neurotransmitters used by these cells. In the present study, ganglion cells identified with a ganglion cell-specific monoclonal antibody (AB5) are shown in separate double-label experiments to be gamma-aminobu...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(88)91498-9
更新日期：1988-01-26 00:00:00
abstract：:The distribution of carbonic anhydrase (CA) activity was determined histochemically using Hansson's cobalt phosphate method in cross-sections of peripheral nerves from rats. As the studied nerves contain either efferent, proprioceptive or exteroceptive myelinated fibres, our survey particularly focused on the question...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(89)90895-0
更新日期：1989-07-17 00:00:00
abstract：:To identify whether altered expressions of voltage gated Ca(2+) channel (VGCC) are linked to inhibitory transmission abnormalities in the gerbil hippocampus, we investigated the effects of GABA receptor or somatostatin receptor (SST) antagonists/agonists on VGCC immunoreactivity in vivo. VGCC immunoreactivities in the...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2006.07.076
更新日期：2006-10-18 00:00:00
abstract：:Cingulate cortex (CC) unit responses to hippocampal volleys and afterdischarges were studied in 4 awake unsedated squirrel monkeys using extracellular microelectrodes. Stimuli were delivered via bipolar electrodes chronically implanted bilaterally in anterior and posterior hippocampus. A total of 412 units in 21 track...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(83)91253-2
更新日期：1983-01-24 00:00:00
abstract：:It has been demonstrated previously that kappa opioid receptor agonists, such as dynorphin, inhibit oxytocin secretion in the rat. To determine whether kappa agonists act directly on oxytocin-containing magnocellular neurons to inhibit hormone secretion, we utilized immunofluorescence to examine the cellular localizat...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(01)02154-0
更新日期：2001-04-13 00:00:00
abstract：:The distribution of radioactivity after injection of [3H]testosterone was studied in the forebrain of adult mice by thaw-mount autoradiography. Nuclear labeling was high in neurons in the dorsal part of the medial nucleus of the amygdala and in the dorsocaudal part of the bed nucleus of the stria terminalis. Low nucle...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(86)90630-x
更新日期：1986-11-12 00:00:00
abstract：:Antisense oligodeoxynucleotide (AS ODN) probes directed against the alpha-subunit of different G-proteins have been used to differentiate feeding responses in rats elicited by different opioid agonists, including morphine, beta-endorphin and dynorphin. Furthermore, antisense probes directed against G(o)alpha, but not ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(02)03361-9
更新日期：2002-11-15 00:00:00
abstract：:Recent work in our laboratory has demonstrated that spontaneous or evoked climbing fiber inputs are associated with an increased responsiveness of Purkinje cells to mossy fiber inputs activated by natural peripheral stimuli. These experiments were designed to test the hypothesis that this increased responsiveness occu...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(84)91027-8
更新日期：1984-08-20 00:00:00
abstract：:The malformative cortical lesions in the cerebral cortex that are characteristic of focal cortical dysplasia type IIb (FCDIIb) and tuberous sclerosis complex (TSC) are well-recognized causes of chronic intractable epilepsy in children. Increasing evidence suggests that extracellular matrix molecules play important rol...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2012.03.009
更新日期：2012-05-09 00:00:00
abstract：:Distribution of somatostatin (SS) immunoreactivity was observed in the preoptic-anterior hypothalamus (PO-AH) of the female rat brain. Six specific rabbit antibodies A (SS-14), B (SS-28), C (SS-28 complete), D (SS 14 and SS-28 both), E (SS preprohormone) and F (GHRH) were used for immunostaining using Avidin-Biotin Co...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(00)03164-4
更新日期：2001-05-11 00:00:00
abstract：:Neuronal expression of c-fos product in the cat rostral ventrolateral medulla was studied by immunohistochemistry. Spinally projecting neurons of the subretrofacial (SRF) nucleus were pre-labeled by retrograde transport of fluorescent latex microspheres from the lumbar cord. In 3 animals which were bled by approximate...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(92)90099-u
更新日期：1992-03-20 00:00:00
abstract：:Two-dimensional electrophoresis of proteins has allowed high resolution analysis of the protein species rapidly transported in the frog sciatic nerve. The 7th, 8th and 9th dorsal root ganglia were selectively labeled with [3H]leucine or [35S]methionine in one compartment of a lucite chamber. Transport of TCA-precipita...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(78)90155-5
更新日期：1978-04-14 00:00:00
abstract：:The effects of diazepam, nitrazepam, clonazepam, and Ro5-4864 on transient (type I) and long-lasting (type II) calcium channels associated with low-affinity benzodiazepine receptors were investigated using the whole-cell patch-clamp technique. Clonazepam (100 microM), a specific agonist for the central-type benzodiaze...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(93)90991-u
更新日期：1993-03-26 00:00:00
abstract：:There have been few reports concerning facilitation and depression in sympathetic ganglia9,17,40. In the present investigation, pairs of excitatory postsynaptic potentials (EPSPs) were recorded intracellularly from bullfrog paravertebral sympathetic ganglia for an analysis of the site and mechanism responsible for the...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(76)90840-4
更新日期：1976-12-10 00:00:00
abstract：:Pouch young opossums aged from 5 to 34 days underwent unilateral eye enucleation and the projection from the remaining eye to the superior colliculus (SC) was explored at maturity by anatomical and physiological methods. The results were compared to data from normal or adult enucleated animals. Analysis of the experim...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0165-3806(86)90236-1
更新日期：1986-06-01 00:00:00
abstract：:This study evaluates the neuroprotective effects of geranylgeranylacetone (GGA), which is known as an antiulcer agent and more recently as a heat-shock and other neuroprotective protein inducer, on secondary degeneration after spinal cord injury in rats. Crush injuries were produced at the T8 level using an extradural...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2005.06.055
更新日期：2005-08-16 00:00:00
abstract：:An initial decrement followed by a significant recovery of choline acetyltransferase and acetylcholinesterase activities in the ipsilateral hippocampus was observed in rats with unilateral, partial transection of the fimbria. Regeneration of hippocampal cholinergic terminals could account for the parallel return towar...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(83)90921-6
更新日期：1983-10-24 00:00:00
abstract：:Whole-cell patch-clamp recordings were undertaken to characterize and compare the membrane properties of deep dorsal horn neurons in transverse slices of rat lumbar spinal cord in two age groups, postnatal days (P) 3-6 and 9-16. In both age groups, significant correlations were observed between membrane time constant ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(97)00578-7
更新日期：1997-09-05 00:00:00
abstract：:Our research group recently presented the distribution of thyrotropin-releasing hormone (TRH) in the chicken brain. In this study we measured somatostatin (SRIH) concentrations in different brain parts and nuclei. The distribution of SRIH and TRH in peripheral tissues was also studied. Although the highest SRIH conten...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(00)02550-6
更新日期：2000-08-11 00:00:00
abstract：:Perfusion of hippocampal slices with normoxic medium containing no added sodium resulted in a rapid loss of the CA1 population spike, with only 35 +/- 10% (S.E.M.) (P < 0.001) recovery after a 15 min exposure. This injury was prevented by the non-competitive N-methyl-D-aspartate (NMDA) antagonist MK-801 (93 +/- 4% rec...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(92)91464-p
更新日期：1992-11-06 00:00:00
abstract：:A major mesolimbic projection towards the hippocampal formation (HF) has been extensively described, but no clear evidence of its dopaminergic content has been demonstrated. In order to evaluate the percentage of dopaminergic (DA) cells of ventral tegmental area (VTA-A10) and adjacent substantia nigra (SN-A9) projecti...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(94)90512-6
更新日期：1994-12-30 00:00:00
abstract：:Using the hippocampal slice preparation, extracellular recordings of CA3 field excitatory postsynaptic potentials (fEPSPs) were performed to assess the effects of adenosine on the induction of mossy fiber long-term potentiation (LTP). When present during tetanization, adenosine (50 microM) significantly suppressed mos...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(91)91061-5
更新日期：1991-03-08 00:00:00
abstract：:Catecholamine concentrations were determined at central and peripheral level in high blood pressure (HBP), low blood pressure (LBP) and random bred (RB) mice. In HBP mice compared to LBP, the noradrenaline concentration was lower in the locus coeruleus, medullary A1-C1 and A2-C2 areas, thoracic spinal cord, posteroven...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(85)90784-x
更新日期：1985-08-05 00:00:00
