Abstract：
:The effects of diazepam, nitrazepam, clonazepam, and Ro5-4864 on transient (type I) and long-lasting (type II) calcium channels associated with low-affinity benzodiazepine receptors were investigated using the whole-cell patch-clamp technique. Clonazepam (100 microM), a specific agonist for the central-type benzodiazepine receptor, reduced transient currents through the type I calcium channel by 40% without affecting long-lasting currents through the type II calcium channel. Diazepam and nitrazepam (100 microM), non-specific agonists for both the central- and peripheral-type benzodiazepine receptors, reduced both transient and long-lasting currents equally by 25-30%. A similar non-selective inhibition was observed by Ro5-4864 (1-10 microM), a specific agonist for the peripheral-type benzodiazepine receptor. It is concluded that the two calcium channel types are regulated differentially by two different kinds of benzodiazepines; central-type for type I channel and peripheral-type for both type I and type II channels.
journal_name
Brain Resjournal_title
Brain researchauthors
Watabe S,Yoshii M,Ogata N,Tsunoo A,Narahashi Tdoi
10.1016/0006-8993(93)90991-usubject
Has Abstractpub_date
1993-03-26 00:00:00pages
244-50issue
2eissn
0006-8993issn
1872-6240pii
0006-8993(93)90991-Ujournal_volume
606pub_type
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