Abstract:
:Studies were undertaken to characterize the binding of [3H]estradiol ([3H]E2) to blood plasma and to brain cytosols collected from perinatal ferrets of both sexes. A dialysis experiment showed that the binding capacity of plasma for [3H]E2 was low in neonatal ferrets. Saturable, high-affinity binding of [3H]E2 to cytosols prepared from ferret hypothalamus + preoptic area (H + POA), basal temporal lobe, and midbrain + brainstem (sexes pooled) was demonstrated 5 days prior to the date of expected parturition (42-day gestation), as well as on the day of birth and on postnatal days 5 and 10. The binding of E2 to cytosols prepared from H + POA and temporal lobe of newborn ferrets met established criteria for estrogen receptors: (a) it was saturable; (b) it had a high affinity (Kd approximately 10(-10) M); (c) it was apparently macromolecular since it migrated in front of a 4.7 S dansyl-bovine serum albumin marker on sucrose gradients of low ionic strength and eluted in the void volume of Sephadex LH-20 columns; (d) it was apparently proteinaceous since binding was destroyed by preincubation with pronase; and (e) it was steroid-specific. Previous research suggests that perinatal exposure to E2 causes neither behavioral defeminization nor masculinization in the developing ferret. The present findings raise the question of whether estrogens, acting via neural receptors, normally affect brain development in ferrets of either sex.
journal_name
Brain Resjournal_title
Brain researchauthors
Holbrook PG,Baum MJdoi
10.1016/0165-3806(83)90075-5subject
Has Abstractpub_date
1983-03-01 00:00:00pages
1-8issue
1eissn
0006-8993issn
1872-6240journal_volume
283pub_type
杂志文章相关文献
BRAIN RESEARCH文献大全abstract::The cellular and intracellular localization of the epsilon-subspecies of protein kinase C (PKC) in the rat brain was demonstrated by immunocytochemistry using specific antibodies against epsilon-PKC. The epsilon-PKC-specific immunoreactivity was most abundant in the hippocampal formation, olfactory tubercle and Callej...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(93)91512-q
更新日期:1993-04-02 00:00:00
abstract::Recently developed antisera selective for angiotensin Type 2 (AT2) receptors were used to localize AT2 receptors in rat brain by immunohistochemistry. While the results from these experiments were largely consistent with previous autoradiographic and radioligand binding analyses of AT2 receptor populations in brain, t...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(94)90794-3
更新日期:1994-10-31 00:00:00
abstract::The astrocyte-derived protein S100B stimulates production of inducible nitric oxide synthase and nitric oxide (NO) in astrocytes [Hu et al., 1996, J. Biol. Chem. 271:2543], but its effect on microglia is not known. In addition, S100B's ability to modulate the activity of other glial activating agents has not been defi...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(99)02251-9
更新日期:2000-01-17 00:00:00
abstract::Retrogradely labeled neurons are observed in the central nervous system of the toad after peripheral application of peroxidase to the anterior and posterior stumps of the VIIIth nerve. These efferent vestibular neurons are localized in the brainstem only ipsilaterally to the treated nerve; they are restricted within a...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(85)91183-7
更新日期:1985-09-30 00:00:00
abstract::The amide-type local anesthetic (LA) lidocaine activates transient receptor potential (TRP) ankyrin-1 (TRPA1) channels to facilitate spontaneous l-glutamate release onto spinal substantia gelatinosa (SG) neurons, which play a crucial role in regulating nociceptive transmission. In contrast, the ester-type LA procaine ...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2016.12.021
更新日期:2017-02-15 00:00:00
abstract::Power spectral analysis of the electroencephalogram (EEG) is a non-invasive method to examine infant brain maturation. Preterm fetal growth restricted (p-FGR) neonates display an altered EEG power spectrum compared to appropriate-for-gestational-age (AGA) peers, suggesting delayed brain maturation. Longitudinal studie...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2017.10.010
更新日期:2018-01-01 00:00:00
abstract::In extracellular recordings from chloral hydrate anesthetized rats the 5-HT3 antagonist, BRL 46470A, failed to prevent or reverse the increase in dopamine cell firing rate produced by systemic or iontophoretically applied morphine. A second 5-HT3 antagonist, tropesitron, was similarly found to be ineffective in antago...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(94)90665-3
更新日期:1994-02-28 00:00:00
abstract::Perinatal exposure to environmental tobacco smoke (ETS) has adverse effects on neurobehavioral development. In the current study, rats were exposed to ETS during gestation, during the early neonatal period, or both. Brains and hearts were examined for alterations in adenylyl cyclase (AC) activity and for changes in be...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(01)02145-x
更新日期:2001-04-13 00:00:00
abstract::The [14C]2-deoxy-D-glucose technique was used to test the effects of central muscarinic stimulation on local cerebral glucose utilization (LCGU) in rats. Systemic administration of the muscarinic agonist oxotremorine (OXO, 0.7 mg/kg, ip) increased LCGU in brain regions involved in motor function: the sensorimotor cort...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(81)91100-8
更新日期:1981-12-07 00:00:00
abstract::Previous studies in our laboratory using rat brain tissue have shown that neuropeptide Y (NPY) can enhance NMDA- and potassium-stimulated dopamine release from various brain regions and that this enhancement is reversed by sigma (sigma) receptor antagonists. In the current study, we sought to determine whether SH-SY5Y...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(00)02722-0
更新日期:2000-09-22 00:00:00
abstract:BACKGROUND:Intermittent hypoxia during sleep (IH), as occurs in sleep disordered breathing (SDB), induces spatial learning deficits associated with regulation of transcription factors associated with learning and memory in the hippocampal CA1 region in rats. high fat refined carbohydrate diet (HF/RC) can induce similar...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2006.03.046
更新日期:2006-05-23 00:00:00
abstract::We have examined the saturation features (Kd and Bmax) of [3H]naloxone binding in rat brain regions in cytoarchitectonically intact tissues of naive, morphine dependent and precipitated withdrawal states using quantitative film autoradiography. Sixty-one saturation experiments in 13 regions of naive rat brains yielded...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(85)91237-5
更新日期:1985-12-23 00:00:00
abstract::Nitric oxide (NO) is a putative neuronal messenger in the olfactory epithelium. We analysed the electrical responses of rat olfactory receptor neurones to NO stimulation in the whole-cell patch clamp mode and found that NO activates the cyclic nucleotide-gated conductance, independent from cyclic GMP. As NO may also o...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(03)02990-1
更新日期:2003-08-01 00:00:00
abstract::Extracellular recordings from the supraoptic nucleus of the rat established that vasopressinergic neurosecretory cells were excited by stimulation of cervical but not abdominal vagal afferents. This response was absent or significantly attenuated after microinjection of gamma-aminobutyric acid into a region of the cau...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(92)91131-w
更新日期:1992-10-30 00:00:00
abstract::The so-called on- and off-cells of the rostral ventromedial medulla (RVM) send their axons to the spinal dorsal horn. Activation of on-cells precedes and coincides with a facilitation, and activation of off-cells coincides with an inhibition, of withdrawal reflexes elicited by noxious agents. Considerable evidence sup...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2018.02.025
更新日期:2018-05-01 00:00:00
abstract::Previous studies have suggested that substance P (SP) plays a critical role in the development of brain oedema and functional deficits following traumatic brain injury and that SP receptor antagonism may improve outcome. No studies have described such a role in ischemic stroke. The present study characterized the effe...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2011.03.066
更新日期:2011-06-01 00:00:00
abstract::Ventilated, chloralose anesthetized cats were subjected to 3.2 atmosphere fluid percussion injury and compared with sham operated controls. In both groups, global cerebral blood flow (CBF), cerebral metabolic rate of oxygen utilization (CMRO2), cerebral metabolic rate of glucose utilization (CMRG1), and 31P magnetic r...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(92)91205-s
更新日期:1992-07-10 00:00:00
abstract::Vascular basement membrane (BM) stabilizes brain vessels and inhibits endothelial cell cycle. Cerebral ischemia causes BM breakdown with the loss of structural BM components including collagens and laminins. In this study, the expression changes of the BM proteoglycan agrin, and the non-structural BM constituent SPARC...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2009.02.062
更新日期:2009-05-07 00:00:00
abstract::Nerve-evoked muscle depolarisation plays an important role in the downregulation of extrasynaptic AChRs which accompanies the increase in synaptic AChR expression at the neuromuscular junction during embryonic development. However, additional mechanisms may be involved in the AChR downregulation. This study provides e...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(98)01313-4
更新日期:1999-02-13 00:00:00
abstract::Responses of subthalamic neurons (STN) to the iontophoretic application of glutamate (Glu), acetylcholine (ACh) and GABA were studied in rats 2 or 3 weeks following bilateral decortication, and were compared to those obtained in normal animals. All the cells recorded in lesioned rats showed a decrease in their spontan...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(87)90077-1
更新日期:1987-02-17 00:00:00
abstract::Extracellular microelectrode recordings were made from a total of 868 neurons in the medullas of cats in regions known to contain high densities of respiratory-related neurons (solitary tract complex, nucleus ambiguus/retroambigualis, lateral tegmental field). Both the discharge patterns and the locations of units wer...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(84)90979-x
更新日期:1984-03-19 00:00:00
abstract::Extracellular and intracellular recordings have been made from non-pyramidal neurones in the rat hippocampus in vivo and in vitro. These cells were situated in the stratum pyramidale but were orthodromically activated with a lower threshold than pyramidal neurones in response to stimulation of the Schaffer collateral/...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(84)91235-6
更新日期:1984-02-20 00:00:00
abstract::The effect of intracerebroventricular administration of low-antinociceptive doses of selective mu- (DAMGO) or delta- (DPDPE) opioid agonists on the dose-dependent antinociceptive effects produced by intrathecal administration of sequentially increasing doses of selective mu-, delta-, or kappa-(U50,488H) opioid agonist...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(92)91449-o
更新日期:1992-11-06 00:00:00
abstract::In adult cats, we have investigated how nerve cross-union between slow and fast muscle affects twitch contraction and half-relaxation times in the presence and absence of motor disuse, produced by complete decerebellation. Cerebellectomy abolished the slowing of cross-innervated fast muscle and diminished the amount o...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(82)90480-2
更新日期:1982-02-18 00:00:00
abstract::Activation of kappa-opioid receptors (KOR) in the medial prefrontal cortex (mPFC) modulates excitatory transmission, which may involve interactions with N-methyl-D-aspartate (NMDA) glutamate receptors. We investigated possible anatomical correlates of this modulation by using dual labeling electron microscopy to exami...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(02)02894-9
更新日期:2002-08-16 00:00:00
abstract::'Feedback' signals from mammalian extrastriate visual cortices are reported to exert primarily an excitatory influence on the classical receptive field (CRF) of neurons in the primary visual cortex (V1). However, given the much larger CRFs of neurons in extrastriate visual cortices it is not yet understood how feedbac...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2010.02.069
更新日期:2010-04-30 00:00:00
abstract::Rats exposed to inescapable tailshock exhibit deficits in learning a simple shuttlebox escape task 24 h later. This syndrome has been termed 'behavioral depression' or 'learned helplessness', and is a model of stress-induced depression. In the present study a significant (25%) decrease in GABA receptor-mediated chlori...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(89)90938-4
更新日期:1989-05-15 00:00:00
abstract::c-Fos protein is a nuclear protein coded by c-fos proto-oncogene subsequent to synaptic activation of the neurons. We used immunohistochemical methods to visualize the expression of c-Fos protein in myenteric neurons of the guinea pig distal colon and examined the effects of the extrinsic autonomic inputs on the enter...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(02)02943-8
更新日期:2002-09-06 00:00:00
abstract::The technique of intracellular recording was used to assess the effect of SR 31742A, a selective sigma receptor ligand, on N-methyl-d-aspartate (NMDA) and (+/-)-alpha-amino-3-hydroxy-5-methylisoxazole-4-propionic acid (AMPA) receptor-mediated responses in pyramidal cells of the rat medial prefrontal cortex in vitro br...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(98)00797-5
更新日期:1998-10-05 00:00:00
abstract::To determine the respiratory effects of inhibiting carbonic anhydrase in brain tissue, we infused acetazolamide (ACZ) into the cisterna magna in anesthetized rats. Three concentrations, 4.5, 45 and 450 microM in artificial cerebrospinal fluid, were infused continuously at 2 microliter/min for 20 min and the responses ...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(90)90056-h
更新日期:1990-06-11 00:00:00