Abstract：
:We have examined the saturation features (Kd and Bmax) of [3H]naloxone binding in rat brain regions in cytoarchitectonically intact tissues of naive, morphine dependent and precipitated withdrawal states using quantitative film autoradiography. Sixty-one saturation experiments in 13 regions of naive rat brains yielded monophasic Eadie-Hofstee plots with a mean (+/- S.D.) Kd of 1.87 +/- 0.87 nM and a mean Bmax (+/- S.D.) of 101 +/- 66 fmol/mg. The 61 Kd values in naive rats described a normal distribution of regional binding affinities that may reflect the biological variation of a single high affinity binding site. Similar studies in the morphine dependent and precipitated withdrawal states showed no apparent changes in either the Kd or Bmax of regional [3H]naloxone binding. The possibility that in vitro preincubation of tissue sections masked potential alterations in [3H]naloxone binding was examined in competition studies of both morphine and naloxone for 2.0 nM [3H]naloxone binding to striatal sections. Preincubation had no effect on the ability of either opiate agonist or antagonist to compete for [3H]naloxone binding in the naive, morphine dependent or precipitated withdrawal states. The regional data comparing Kd and Bmax for [3H]naloxone binding in naive, morphine dependent and precipitated withdrawal states eliminated the possibility that previous efforts to demonstrate alterations in opiate binding were confounded by homogenization and/or limitations of gross dissection. Our data suggest that either potential changes in antagonist binding in dependent and withdrawal states can only be demonstrated under strict in vivo conditions or that post-binding transduction mechanisms are the sites of adaptive changes in naloxone sensitivity in the states of opiate dependence and withdrawal.
journal_name
Brain Resjournal_title
Brain researchauthors
Geary WA 2nd,Wooten GFdoi
10.1016/0006-8993(85)91237-5subject
Has Abstractpub_date
1985-12-23 00:00:00pages
214-23issue
1-2eissn
0006-8993issn
1872-6240pii
0006-8993(85)91237-5journal_volume
360pub_type
杂志文章相关文献
BRAIN RESEARCH文献大全abstract：:The present study was designed to investigate the distribution of serotonin 5-HT1A receptor protein (5-HT1A-immunoreactivity) and its localization within cortical pyramidal neurons of the rat cingulate cortex. This experimental direction was inspired by recent data showing the role of 5-HT1A receptors in the pathology...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(03)03352-3
更新日期：2003-10-31 00:00:00
abstract：:Chick neural cultures were used to study effects of insulin, thyrotropin releasing hormone, growth hormone and glucagon on myelin lipid synthesis in vitro. The incorporation of [3H]galactose into myelin associated lipids such as cerebroside and sulfatide was used as an index for various hormonal effects on myelination...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(86)90831-0
更新日期：1986-04-16 00:00:00
abstract：:The present investigation is aimed at studying the influence of the striate cortex (area 17 or V1) upon responses of area 18a. Electrical activity was recorded from neurons in area 18a through glass micropipettes. At the same time lidocaine hydrochloride was injected in the same retinotopic register of area 17. Forty ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(90)91371-m
更新日期：1990-03-05 00:00:00
abstract：:Developmental differences (9- to 15-year-olds) in effective connectivity in left hemisphere regions were examined using dynamic causal modeling (DCM) of functional magnetic resonance imaging (fMRI) data. Children completed spelling tasks in the visual and auditory modalities in which they were asked to determine if tw...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2007.10.080
更新日期：2008-01-16 00:00:00
abstract：:Effects of alpha-adrenoceptor agents on electrophysiologically and immunohistochemically identified supraoptic nucleus (SON) vasopressin (VP) units were investigated by intracarotid infusion. Clonidine, an alpha 2-adrenoceptor agonist always excited SON units and alpha 2-adrenoceptor antagonists consistently inhibited...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(86)91526-x
更新日期：1986-12-10 00:00:00
abstract：:alpha-Bungarotoxin (alpha-BuTX) binds in a saturable and practically irreversible fashion to membrane-associated receptors in the ciliary ganglion of the adult chick. The binding of toxin to receptors is competitively inhibited by nicotinic cholinergic ligands, and for these properties the receptors are regarded as ac...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(78)91130-7
更新日期：1978-09-15 00:00:00
abstract：:Intra- and interhemispheric connections between the anterior ectosylvian visual area (AEV) and other visual cortical areas including the lateral suprasylvian (LSS) were examined in the cat using the retrograde double-label fluorescence technique. The areal and laminar distributions of labeled neurons were mapped follo...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(85)90189-1
更新日期：1985-11-18 00:00:00
abstract：:Bcl-2 has been hypothesized to regulate many cellular functions in addition to its well-characterized role in the prevention of programmed cell death. To understand the role of Bcl-2 in regulating cell morphology and to explore the mechanism of this effect, we examined the effects of Bcl-2 overexpression on the morpho...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2006.07.017
更新日期：2006-09-27 00:00:00
abstract：:The bag cell neurons of Aplysia are capable of generating an afterdischarge, which, in vivo, triggers egg-laying behavior. Pharmacologic elevation of cyclic AMP levels in isolated bag cell neurons has been shown to initiate repetitive firming similar to that seen during an afterdischarge, and to decrease outward curre...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(90)91733-w
更新日期：1990-11-05 00:00:00
abstract：:In this study we used two lines of transgenic mice overexpressing tumor necrosis factor alpha (TNF-alpha) in the central nervous system (CNS), one characterized by reactive gliosis, inflammatory demyelination and neurological deficits (Tg6074) the other showing no neurological or phenotypical alterations (TgK3) to inv...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(99)01748-5
更新日期：1999-09-04 00:00:00
abstract：:The objective of this study was to further investigate neurochemicals which might modulate congenital differences in sensitivity to the acute and chronic effects of ethanol. Catecholamine levels were measured in the cortex, hippocampus, midbrain and cerebellum of long-sleep (LS) and short-sleep (SS) mice. These measur...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(91)91513-z
更新日期：1991-11-01 00:00:00
abstract：:In rats with unilateral 6-hydroxydopamine lesions of the substantia nigra, a specific D1 dopamine receptor agonist, SKF 38393A, at a dose that does not itself produce turning, significantly increased the contralateral rotation observed following a low dose of the specific D2 agonist LY 171555. Doses of SKF 38393A or t...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(86)91178-9
更新日期：1986-10-08 00:00:00
abstract：UNLABELLED:The hypothesis that the lower midbrain, specifically in and around the retrorubral field (RRF) and/or rubrospinal tract (rs), contains a tonic inhibitory mechanism on non-shivering thermogenesis (NST) was examined in urethane-anesthetized rats. It has been proposed that removal of the tonic inhibitory mechan...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(99)01532-2
更新日期：1999-07-03 00:00:00
abstract：:In this study we investigated the functional connectivity in 23 Mild TBI (mTBI) patients with and without memory complaints using resting state fMRI in the sub-acute stage of injury as well as a group of control participants. Results indicate that mTBI patients with memory complaints performed significantly worse than...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2013.08.034
更新日期：2013-11-06 00:00:00
abstract：:Following acute intracerebroventricular injections of 1 ng of apamin and chronic apamin infusion (0.4 ng/microl, 0.5 microl/h, 14 days), the rat brains exhibited bilateral damage only in the cerebellum. The argyrophilic cells were Purkinje cells in copula pyramis, flocculus, paraflocculus, and paramedian lobules. Thes...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(97)01165-7
更新日期：1997-12-19 00:00:00
abstract：:Glucose inhibits ∼60% of lateral hypothalamic (LH) orexin neurons. Fasting increases the activation of LH orexin glucose-inhibited (GI) neurons in low glucose. Increases in spontaneous glutamate excitatory postsynaptic currents (sEPSCs) onto putative VTA DA neurons in low glucose are orexin dependent (Sheng et al., 20...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2018.05.025
更新日期：2020-03-15 00:00:00
abstract：:The present study utilized the [14C]2-deoxyglucose (2-DG) cell labeling procedure to characterize a functional pathway from the prefrontal cortex (Pfc) and mediodorsal thalamic nucleus (MD) to the hypothalamus. Rats were injected with 2-DG prior to a 45 min experimental paradigm consisting of alternating 30 s on-off p...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(84)90151-3
更新日期：1984-09-24 00:00:00
abstract：:From 4 or 5 postnatal days, rat pups were either reared by their mothers (MR) or artificially reared (AR) without their mothers by infusion of 'milk' through a gastric cannula. In AR, but not MR, pups the left eye was more likely to open first than the right eye (P less than 0.001). The left-biassed eye-opening of AR ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0165-3806(86)90073-8
更新日期：1986-07-01 00:00:00
abstract：:Experiments were performed which demonstrate that low power, pulsed microwave exposure induces a significant increase in local cerebral blood flow within the brain of the conscious rat. Previous studies purporting to examine changes in brain vascular permeability induced by microwave radiation must be re-evaluated in ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(81)90668-5
更新日期：1981-01-05 00:00:00
abstract：:In an autopsy study on 29 male Aborigines and 57 male Caucasians, macroscopic and microscopic parameters of postnatal cerebellar development were determined and described by mathematic growth functions. For the whole cerebellum and its cortical layers volume measurements revealed delayed maturation for Aborigines comp...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(89)90782-8
更新日期：1989-10-16 00:00:00
abstract：:Endogenous opioid systems (i.e. opioids and opioid receptors) play a role in regulating neural development. Using the cerebellar cortex of 6-day-old rats, the most potent opioid peptides involved with cell proliferation were assessed. In both the external germinal (granule) layer (EGL), a germinative matrix giving ris...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(91)91585-o
更新日期：1991-03-01 00:00:00
abstract：:Mild acute hypertension was induced in rats pharmacologically and non-pharmacologically with metaraminol, angiotensin II or bilateral vagotomy. The extraction fraction of water (Ew) was measured in both hypertensive and normotensive animals. It was demonstrated that cerebral blood flow did not change during hypertensi...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(84)91069-2
更新日期：1984-08-13 00:00:00
abstract：:Neurons with intrinsic pacemaker activity and presumed sympathoexcitatory function were recorded in rat tissue slices within the confines of the rostroventrolateral reticular nucleus (RVL). These cells were excited in dose-dependent fashion by arginine vasopressin (AVP, 10(8)-10(6) M) but not by oxytocin (up to 10(7) ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(89)90909-8
更新日期：1989-07-17 00:00:00
abstract：:Pregnenolone sulfate (PS) is an endogenous neurosteroid known to antagonize GABA(A) receptor-mediated inhibitory responses and potentiate NMDA receptor-mediated excitatory responses in vitro. To assess the actions of the steroid as a modulator of seizure susceptibility in vivo, PS (30-300 nmol) was administered intrac...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(99)01287-1
更新日期：1999-06-12 00:00:00
abstract：:Histone proteins from purified nuclei of neonatal and adult mouse retinas were analyzed and compared utilizing SDS-polyacrylamide gel electrophoresis. Identical procedures were applied to examine the histones extracted from the brains of the same animals. In the newborn and mature retina and brain, 8 histone fractions...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0165-3806(87)90150-7
更新日期：1987-06-01 00:00:00
abstract：:The addition of L-glutamic acid (Glu) alone, both Glu and glycine (Gly) or Glu/Gly/spermidine (SPD) was effective in potentiating [3H]5-methyl-10,11-dihydro-5H-dibenzo[a,d]cyclohepten-5,10- imine (MK-801) binding before equilibrium to an ion channel associated with the N-methyl-D-aspartate (NMDA) receptor complex in b...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(94)91029-4
更新日期：1994-02-14 00:00:00
abstract：:Clozapine therapy has been associated with a high degree of urinary disturbances. The purpose of this study is to examine the effect of clozapine on urodynamic parameters and on the activity of the external urethral sphincter in anesthetized rats. Single cystometrograms (CMG) were performed on urethane-anesthetized fe...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(01)02352-6
更新日期：2001-05-18 00:00:00
abstract：:Postsynaptic potentials were evoked in neostriatal neurons by stimulation of the ipsilateral and contralateral medial agranular frontal cortical field (AGm) in the rat. This cortical region is known to project bilaterally to the dorsal lateral head of the caudate-putamen of rats. Ipsilateral stimulation of AGm should ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(86)91593-3
更新日期：1986-03-05 00:00:00
abstract：:The present study examined the influences of dopamine (DA) receptor stimulation on enkephalin (Met5-enkephalin; ME) and tachykinin (substance P; SP) systems of basal ganglia of Sprague-Dawley rats, lesioned as neonates with 6-hydroxydopamine (6-OHDA). It has been proposed that the neonatal 6-OHDA-lesioned rat could se...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(89)90305-3
更新日期：1989-10-23 00:00:00
abstract：:Hindpaw injections of carrageenan (CARRA) decreased paw withdrawal latencies to radiant heat and increased hindpaw edema. At the peak of hyperalgesia, the content of immunoreactive guanosine 3',5'-cyclic monophosphate (i-cGMP) was increased, while immunoreactive adenosine 3',5'-cyclic monophosphate (i-cAMP) was decrea...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(94)90066-3
更新日期：1994-05-16 00:00:00
