Abstract：
:The protein inhibitor of nitric oxide synthase (PIN) was independently identified as an inhibitor of nitric oxide (NO) produced by neuronal nitric oxide synthase (nNOS), and as a member of the cellular dynein light chain family, dynein light chain 8 (LC8), responsible for intracellular protein trafficking. Mast cells (MC) are involved in several homeostatic and pathological processes and can be regulated by NO. This study describes the expression of PIN/LC8 in the human MC line HMC-1. We also studied if PIN/LC8 binds nNOS, and what role this might have in leukotriene (LT) production. We found that PIN/LC8 mRNA and protein was expressed in HMC-1. Using a GST-PIN construct, we showed PIN binds to nNOS, but not endothelial (e)NOS in HMC-1; in our studies HMC-1 did not express inducible (i)NOS. Intracellular delivery of anti-PIN/LC8 antibody enhanced ionophore (A23187)-induced LT production through an unknown mechanism. Thus we established for the first time expression of PIN/LC8 in human MC, its ability to bind nNOS, and the effect that blocking it has on LT production in a human MC lines.
journal_name
Life Scijournal_title
Life sciencesauthors
McCauley SD,Gilchrist M,Befus ADdoi
10.1016/j.lfs.2006.11.025subject
Has Abstractpub_date
2007-02-13 00:00:00pages
959-64issue
10eissn
0024-3205issn
1879-0631pii
S0024-3205(06)00892-7journal_volume
80pub_type
杂志文章相关文献
LIFE SCIENCES文献大全abstract：:Cumulative damage in cells from aged people could lead to a greater fragility against acute oxidative stress. The effects of acute oxidative stress on cell viability, cAMP and cGMP concentrations, and protein synthesis rates were studied in lymphocytes from 25 young and 26 elderly subjects. Lymphocytes were exposed to...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(98)00231-8
更新日期：1998-01-01 00:00:00
abstract：:This study examines the influence of ovariectomy and administration of a pharmacologic dose of estradiol on amylase release from isolated-dispersed rat pancreatic acini and cholecystokinin receptors on rat acinar cell membranes. Rats were sham ovariectomized (intact) or ovariectomized (Ovx) and 21 day timed release pe...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(91)90281-f
更新日期：1991-01-01 00:00:00
abstract：:As the relationship between emotional behavior and electrocardiographic (ECG) change induced by hypothalamic stimulation is poorly understood, eighty-four points in various areas within the hypothalamus in conscious cats were stimulated electrically through chronically implanted electrodes, the objective being to clar...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(85)90494-1
更新日期：1985-03-18 00:00:00
abstract：:Calcium spirulan (Ca-SP) is a novel sulfated polysaccharide isolated from a blue-green alga Spirulina platensis. Ca-SP inhibits thrombin by activation of heparin cofactor II. Therefore, it could serve as an origin of anti-atherogenic medicines. Since maintenance of vascular endothelial cell monolayers is important for...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(01)01555-7
更新日期：2002-03-08 00:00:00
abstract：:Apigenin is one type of bioflavonoid widely found in citrus fruits, which possesses a variety of pharmacological actions on the central nervous system. A previous study showed that acute intraperitoneal administration of apigenin had antidepressant-like effects in the forced swimming test (FST) in ddY mice. To better ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2008.01.007
更新日期：2008-03-26 00:00:00
abstract：:Inflammation, inflammatory mediators, cyclooxygenase (COX)-2, and inducible nitric oxide (iNOS) are all influenced by age-related oxidative status. To investigate the effect of dietary fish oil (FO) and calorie restriction (CR) on oxidative stress-related inflammatory status with age, (NZB/NZW) F1 (B/W) mice were fed ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2005.10.034
更新日期：2006-04-18 00:00:00
abstract：:The mechanism of fibrate-induced myopathy was investigated in this report. When clofibrate (30 to 300 microM) was applied to L6 rat skeletal myoblasts, dose-dependently apoptosis was observed within 24 h. In the apoptotic myoblasts, a caspase-12 cleavage was observed at 2 h and with following caspases-9 and -3-related...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2005.08.003
更新日期：2006-03-13 00:00:00
abstract：:Inositol hexaphosphate (InsP6 or IP6) is ubiquitous. At 10 microM to 1 mM concentrations, IP6 and its lower phosphorylated forms (IP(1-5)) as well as inositol (Ins) are contained in most mammalian cells, wherein they are important in regulating vital cellular functions such as signal transduction, cell proliferation a...
journal_title：Life sciences
pub_type： 杂志文章,评审
doi：10.1016/s0024-3205(97)00092-1
更新日期：1997-01-01 00:00:00
abstract：AIMS:Acetylcholine (ACh) has been shown to increase ion and water excretion in the kidneys, resulting in hypotension. However, no evidence of renal parasympathetic innervation has been shown, and the source of ACh acting on nephrons is still unknown. The aim of the present study was to identify ACh-producing cells in t...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2011.07.011
更新日期：2011-09-12 00:00:00
abstract：:In some cell systems muscarinic receptor stimulation can induce proliferation or transformation. This phenomenon is subtype-specific (only m1 and m3 receptors are effective) and cell type dependent. In 1321N1 astrocytoma cells activation of m3 receptors stimulates phospholipase C, but does not induce DNA synthesis. In...
journal_title：Life sciences
pub_type： 杂志文章,评审
doi：
更新日期：1997-01-01 00:00:00
abstract：:We assessed the anxiolytic effect of Kami-Shoyo-San (Jia-wei-xiao-yao-san; TJ-24), one of a traditional Chinese herbal medicine used for the treatment of menopausal anxiety, by the social interaction (SI) test in male mice. Acute administration of TJ-24 (25-100 mg/kg, p.o.), as well as the gamma-amino-butyric acidA/be...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(01)01290-5
更新日期：2001-09-21 00:00:00
abstract：:The immunosuppressive effects of microcolin A, a lipopeptide extracted from the marine blue green alga Lyngbya majuscula were investigated. Microcolin A suppressed concanavalin A (IC50 = 5.8 nM), phytohemagglutinin (IC50 = 12.5 nM) and lipopolysaccharide (IC50 = 8.0 nM) induced proliferation of murine splenocytes. Mix...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(96)00645-5
更新日期：1997-01-01 00:00:00
abstract：AIMS:The present study aimed to verify changes in cerebellar and plasmatic expression of miRNAs after the chronic consumption of ethanol and caffeine in the UChB rat, an experimental model for alcoholism. MATERIAL AND METHODS:Male rats at 5 months of age, were divided into the following groups (n = 10/group): 1. Ethan...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2019.05.016
更新日期：2019-07-15 00:00:00
abstract：:Defects in the mitochondrial genome have been associated with Parkinson's and Alzheimer's disease, and apoptosis can be triggered by the presence of energetically compromised mitochondria. Thus, in this study we have examined whether the divalent cations Cu2+ and Mn2+ could influence mitochondrial function in vitro. M...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(01)00948-1
更新日期：2001-02-23 00:00:00
abstract：:The effects of chronic treatment with piracetam (100 mg/Kg) plus choline (100 mg/Kg) on rabbits' hippocampal electrical activity and behavior have been studied. Animals were exposed to a neutral environment, in the absence of any external stimulus, and then to an object and a stuffed sparrow-hawk. Results show a drug ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(84)90189-9
更新日期：1984-09-10 00:00:00
abstract：BACKGROUND:Cardiovascular disease (CVD) is the leading cause of mortality worldwide. Cardiac fibrosis is the scarring process occurs commonly with CVDs impairing the function and structure of heart. Herein, we investigated the role of circPAN3 in the pathogenesis of cardiac fibrosis. METHODS:A rat myocardial infarctio...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2020.118015
更新日期：2020-09-15 00:00:00
abstract：AIMS:Endothelial dysfunction is a key triggering event in the development of cardiovascular diseases and the current study explored this phenomenon in the context of inflammation, apoptosis, reactive oxygen species (ROS) and the phosphatidylinositol 3-kinase (PI3K)/Akt signaling pathway during chronic diabetes. MAIN M...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2010.03.019
更新日期：2010-06-05 00:00:00
abstract：:Identifying factors that modulate cocaine self-administration is fundamental to the development of effective strategies to treat and prevent cocaine abuse. In the present study, the influence of an alternative reinforcer on the probability of cocaine use was examined in four adult humans under controlled laboratory co...
journal_title：Life sciences
pub_type： 临床试验,杂志文章
doi：10.1016/0024-3205(94)00878-7
更新日期：1994-01-01 00:00:00
abstract：:Ornithine decarboxylase (ODC) activity increases during many growth responses. Some cells and tissues in culture also exhibit elevated enzyme activity with decreasing osmolality of the culture medium. We have found that this also occurs with uterine tissue from ovariectomized rats. Organ culture incubation under hypot...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(84)90551-4
更新日期：1984-03-26 00:00:00
abstract：:Sphinganine (SP) pre-treatment potentiated the retinoic acid (RA)-induced (4-96h exposures) differentiation and increase of alkaline phosphatase (ALP) activity. A higher percentage of SP pre-treated cells in RA exposures resembled mature myelocytes or granulocytes; greater increase in ALP activity was observed. In cel...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(93)90688-y
更新日期：1993-01-01 00:00:00
abstract：AIM:Muscle atrophy is a common symptom after nerve denervation. Myostatin propeptide, a precursor of myostatin, has been documented to improve muscle growth. However, the mechanism underlying the muscle atrophy attenuation effects of myostatin propeptide in muscles and the changes in gene expression are not well establ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2015.12.056
更新日期：2016-02-01 00:00:00
abstract：:Nuclear factor erythroid 2-related factor 2 (Nrf-2) binds to a specific polyamine responsive element (PRE) in the promoter region of the spermidine-spermine acetyltransferase (SSAT) gene, a key component of the polyamine catabolic pathway. Regulation of SSAT gene transcription requires the additional interaction of Nr...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2006.03.042
更新日期：2006-08-22 00:00:00
abstract：:Experiments were undertaken to examine the characteristics of the adrenergic receptor-coupled cAMP system in rat brain slices. It was found that the potentiation of isoproterenol-stimulated cAMP accumulation by 6-fluoronorepinephrine, an alpha-adrenergic agonist, is largely dependent upon the degree of beta-receptor o...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(85)90129-8
更新日期：1985-09-30 00:00:00
abstract：:This study assessed the effects of acute intravenous L-tryptophan (neutral amino acid precursor for serotonin) administration on cocaine-induced dopaminergic responses. Male Sprague-Dawley rats were surgically implanted with guide cannulas in the nucleus accumbens 5 days prior to the study and with vascular catheters ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(01)01276-0
更新日期：2001-09-07 00:00:00
abstract：AIMS:Oxidative stress and inflammatory response are major factors causing several tissue injuries in intestinal ischemia and reperfusion (I/R). Agmatine has been reported to attenuate I/R injury of various organs. The present study aims to analyze the possible protective effects of agmatine on intestinal I/R injury in ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2017.08.032
更新日期：2017-11-15 00:00:00
abstract：:In primary cultured bovine adrenal chromaffin cells (BACC), pituitary adenylate cyclase activating polypeptide 1-38 (PACAP) produced a dose related increase in tyrosine hydroxylase (TH) Vmax when measured 48 hours after the beginning of the treatment; a significant increase was observed with 0.5 nM and the maximal ind...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(94)00614-8
更新日期：1994-01-01 00:00:00
abstract：:The flavonoid quercetin exhibited a biphasic effect on calcium and phospholipid-dependent protein kinase (protein kinase C) activity from rat brain and pig thyroid. At a low concentration (10(-7) M) quercetin stimulated the enzyme activity whereas at higher concentrations quercetin was inhibitory. By contrast the synt...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(89)90450-5
更新日期：1989-01-01 00:00:00
abstract：:Several neuronal cell lines were screened for endogenous expression of neuronal nitric oxide synthase (nNOS) protein using Western blot analysis. Detectable levels of the nNOS protein were evident in the SK-N-SH, SH-SY5Y, and N1E-115 neuroblastoma cell lines, as well as the NG108-15 neuroblastoma x glioma hybrid. Only...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(02)01732-0
更新日期：2002-07-05 00:00:00
abstract：:Systemic administration of the selective D1 antagonist, SCH 23390, caused significant motor changes in healthy African green monkeys. The effects included the parkinsonian signs of motor freezing, incoordination, bradykinesia, poverty of movement, tremor and depressed blink rate. SCH 23390 administered to MPTP-treated...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(91)90299-q
更新日期：1991-01-01 00:00:00
abstract：:In recent years, natural products have increasingly attracted more attention because of their potential anticancer activity and low intrinsic toxicity. Hispidulin is a natural flavonoid with a wide range of biological activities, including anti-inflammatory, antifungal, antiplatelet, anticonvulsant, anti-osteoporotic,...
journal_title：Life sciences
pub_type： 杂志文章,评审
doi：10.1016/j.lfs.2020.118395
更新日期：2020-10-15 00:00:00
