Abstract:
:In primary cultured bovine adrenal chromaffin cells (BACC), pituitary adenylate cyclase activating polypeptide 1-38 (PACAP) produced a dose related increase in tyrosine hydroxylase (TH) Vmax when measured 48 hours after the beginning of the treatment; a significant increase was observed with 0.5 nM and the maximal induction of close to 2.5-fold was found with 0.1 microM PACAP. The potency of PACAP was nearly 3 orders of magnitude greater than forskolin and VIP in inducing TH activity. These effects were preceded by an increase in TH mRNA levels, that started 2 hours after treatment and peaked 12 hours later. The presence of the phosphodiesterase inhibitor HL 725 further increased the stimulation of TH activity by PACAP, indicating that this activation was mediated via a cascade of events initiated by cAMP. Nicotine (1 microM) failed to increase TH activity significantly, however, when added in association with PACAP, a statistically significant increase of TH was elicited with peptide concentrations 5 times lower (0.1 nM) than the threshold dose of the peptide. The stimulation of nicotinic receptors facilitates the TH induction elicited by PACAP.
journal_name
Life Scijournal_title
Life sciencesauthors
Rius RA,Guidotti A,Costa Edoi
10.1016/0024-3205(94)00614-8subject
Has Abstractpub_date
1994-01-01 00:00:00pages
1735-43issue
22eissn
0024-3205issn
1879-0631pii
0024-3205(94)00614-8journal_volume
54pub_type
杂志文章相关文献
LIFE SCIENCES文献大全abstract::Effects of amphetamine on concentrations of dopamine, norepinephrine and serotonin in several monoamine-containing cell body and terminal regions were examined left and right separately in rats. Results suggest that amphetamine reduced the L-R asymmetry of most of these measures, and this effect is more significant in...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(87)90334-1
更新日期:1987-04-06 00:00:00
abstract::We investigated whether calcium channel antagonists would alter the induction of nitric oxide (NO) synthesis by bacterial lipopolysaccharide (LPS) alone or in combination with interferon-gamma (IFN gamma) in cultured J774 macrophages, rat vascular smooth muscle cells, rat renal mesangial cells, and rat cardiac myocyte...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(95)02163-d
更新日期:1995-01-01 00:00:00
abstract::Karyopherin α2 (KPNA2, also known as importinα-1), a member of the nuclear transporter family, is involved in the nucleocytoplasmic transport pathway of a variety of tumor-associated proteins. Recent studies have found that KPNA2 is overexpressed in various cancers, which is associated with poor prognosis. In addition...
journal_title:Life sciences
pub_type: 杂志文章,评审
doi:10.1016/j.lfs.2019.117140
更新日期:2020-01-15 00:00:00
abstract:AIMS:With the purpose of exploring combinatorial options that could enhance the bactericide efficacy of linezolid against Gram-negative bacteria, we assessed the extent of combination of nano-silver and linezolid. MAIN METHODS:In this study, we selected Escherichia coli MTCC 443 as a model to study the combinatorial e...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2019.05.072
更新日期:2019-08-01 00:00:00
abstract::The decrease of m-AChR density observed in neurodegenerative disorders has generated considerable interest in non-invasive mapping of muscarinic acetylcholine receptors (m-AChR) in the central nervous system. The aim of our study was to evaluate the selectivity of 4-bromodexetimide for the M1, M2, M3 and M4 m-AChR sub...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(96)00212-3
更新日期:1996-01-01 00:00:00
abstract::Despite the fact that numerous studies have been published regarding the possible presence in plasma of an endogenous Na-K pump inhibitor with a digitalis-like structure in essential hypertension, very little is known about this factor in heart disease in general, and in situations characterized by low cardiac output....
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(90)90295-3
更新日期:1990-01-01 00:00:00
abstract:AIMS AND METHODS:We previously reported that angiotensin II (AngII) restores the vascular reactivity diminished by hemorrhagic shock. In this study, we investigated whether the beneficial effects of AngII are related to regulation of gap junctions (GJs) and connexin43 (Cx43), and the implication of MAPK signaling and m...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2018.11.019
更新日期:2019-01-01 00:00:00
abstract:AIM:Myokines are associated with regulation of bone and muscle mass. However, limited information is available regarding the impact of myokines on glucocorticoid (GC) mediated adverse effects on the musculoskeletal system. This study investigates the role of myokine fibroblast growth factor-2 (FGF-2) in regulating GC-i...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2019.05.022
更新日期:2019-07-15 00:00:00
abstract::We used a sensitive and specific radioimmunoassay for acetylcholine (ACh), and detected significant amounts of ACh in the blood of various mammals, including humans. About 60% of human blood ACh was localized in mononuclear leukocytes. Human leukemic T-cell lines, used as T-lymphocyte models, contained both ACh and ch...
journal_title:Life sciences
pub_type: 杂志文章,评审
doi:10.1016/s0024-3205(98)00131-3
更新日期:1998-01-01 00:00:00
abstract::Vascular inflammation induced by the proinflammatory cytokine/NF-kappaB pathway is one of the key mechanisms in the development of atherosclerosis. Peroxisome proliferators-activated receptor-gamma (PPARgamma) plays an important role in the prevention of arterial inflammation and formation of atherogenesis. Herein we ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2006.08.030
更新日期:2006-12-14 00:00:00
abstract::In saturation binding experiments, (+)pentazocine, (+)3-(3-hydroxyphenyl)-N-propylpiperidine (3-PPP), haloperidol and rimcazole did not inhibit the binding of [3H]DTG in a purely competitive fashion. Although Scatchard analysis indicated that [3H]DTG bound to a single site, the inhibition curves of some, but not all, ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(92)90255-n
更新日期:1992-01-01 00:00:00
abstract::Maternal malnutrition is known to impair fetal growth and predispose to the development of hypertension and type 2 diabetes. Recently, studies have demonstrated that intrauterine malnutrition is followed later in male offspring by oxidative stress characterized by increased superoxide generation due to activation of N...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2006.10.028
更新日期:2007-01-30 00:00:00
abstract::The thyrotropin receptor is proposed to contain both a glycoprotein and a ganglioside component. Monoclonal antibodies have been developed against soluble thyroid TSH receptor preparations and using Graves' lymphocytes. These show that initial recognition of thyrotropin requires the glycoprotein component, but that mo...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(83)90170-4
更新日期:1983-01-03 00:00:00
abstract::Aim Determine changes in the expressions of the ion channel-TRPV1-and neuropeptides-NKA, NKB, calcitonin gene-related peptide (CGRP), and SP-in 14-, 21-, and 42-day-old rats after inhaling 1.5% and 2.6% sevoflurane. MAIN METHODS:A small in-house inhalation anesthesia chamber was designed to allow 14-, 21-, and 42-day...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2020.117472
更新日期:2020-05-15 00:00:00
abstract::Effects of hexamethonium (C6) on the excitability of sympathetic ganglion cells were examined by means of intracellular recording. When DC currents were injected, high concentrations of C6 significantly augmented the repetitive firing of the cells without any change in threshold voltage for initiation of the spike. Th...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(85)90324-8
更新日期:1985-06-17 00:00:00
abstract::We investigated the effect of social isolation stress on the formation of experimental liver metastasis resulted from intraportal vein (i.p.v.) injection of colon 26-L5 carcinoma cells in male Balb/c mice, and elucidated some of the underlying mechanism involving the effects of this stress on cellular immunity. Increa...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(00)00506-3
更新日期:2000-03-31 00:00:00
abstract::Behavioral Hypersensitivity (BH) to dopamine agonists occurs following chronic treatment with most neuroleptics including haloperidol. In the present study we observed that the concurrent administration of thioridazine and haloperidol prevented the development of BH. In contrast, another neuroleptic, fluphenazine, coa...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(86)90120-7
更新日期:1986-05-12 00:00:00
abstract:AIMS:Oxidative stress is involved in cholestasis-induced hepatic damage. Therefore, antioxidant therapy is a recommended therapeutic strategy. Studies have illustrated that chromium can enhance antioxidative capacity leading to a resolution of oxidative stress. The aim of this study was to assess whether chromium has p...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2009.02.003
更新日期:2009-04-24 00:00:00
abstract::Microvascular changes in the brain are significant causes of cerebral edema and ischemia injury. A number of studies suggest that angiotensin (Ang) II may be involved in the initiation and regulation of processes occurring in brain ischemia. We recently reported that Ang II injures brain microvascular endothelial cell...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2005.06.049
更新日期:2006-02-16 00:00:00
abstract::The effects of nineteen AHPA* derivatives were examined on morphine analgesia by tail-flick test in rats and on enkephalinase inhibition which was based on the formation of tyrosyl-glycyl-glycine from met-enkephalin. The correlation between the enhancement of morphine analgesia in vivo and enkephalinase inhibition in ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(82)90308-3
更新日期:1982-05-17 00:00:00
abstract::The Ca2+ ionophore A23187 (0.2-5 microM) stimulates the phosphorylation of the substrates of protein kinase C (40,000 dalton protein) and myosin light chain kinase (20,000 dalton protein) in the presence or absence of cyclooxygenase inhibitors. In the presence of cyclooxygenase inhibitors or millimolar Ca2+ there is n...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(86)90024-x
更新日期:1986-08-25 00:00:00
abstract::Fenfluramine, a drug that induces increased synaptic serotonin, was used to train Fawn-Hooded rats in a drug discrimination paradigm. This strain of rats is thought to possess a genetic serotonin storage abnormality. The intent of the study was to see if the Fawn-Hooded rat was similar or dissimilar to the more freque...
journal_title:Life sciences
pub_type: 杂志文章
doi:
更新日期:1997-01-01 00:00:00
abstract::To assess the effect of pharmacological dose of melatonin on bone metabolism in ovariectomized rats, urinary deoxypyridinoline (a marker of bone resorption) and calcium excretion, circulating levels of calcium, phosphorus and bone alkaline phosphatase activity (a marker of bone formation), and bone mineral density (BM...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(01)01431-x
更新日期:2001-12-21 00:00:00
abstract:AIMS:This study aims to develop and evaluate oleuropein loaded surface functionalized folate-targeted - PEG liposomes for the effective management of prostate cancer in an animal model. MATERIALS AND METHODS:Film hydration-cum-extrusion technique was used to produce liposomes. Particle size, entrapment efficiency, dru...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2019.01.053
更新日期:2019-03-01 00:00:00
abstract::SDZ PGU 693 acts as a hypoglycemic agent by stimulating glucose utilisation in insulin-sensitive peripheral tissues, such as skeletal muscle and fat. In a 28 day toxicity study the compound was found to induce hepatocellular hypertrophy in Wistar rats treated with 300 mg/kg/day. To gain insights into the pathomechanis...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(98)00509-8
更新日期:1998-01-01 00:00:00
abstract::The retina contains three catecholamine neurotransmitters: dopamine (DA); norepinephrine (NE); and epinephrine (EPI). DA and EPI appear to be associated with separate amacrine neurons that directly participate in the visual process. NE, in contrast, appears to be associated primarily with the sympathetic nerves that i...
journal_title:Life sciences
pub_type: 杂志文章,评审
doi:10.1016/0024-3205(84)90184-x
更新日期:1984-09-10 00:00:00
abstract:AIMS:Although dexamethasone (Dex) is used widely as an anti-inflammatory and immunosuppressive drug, Dex appears to have severe side-effects, including osteoporosis. This study determined the effects of Dex on duodenal and renal expressions of the calcium-processing genes transient receptor potential cation channel, su...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2009.05.013
更新日期:2009-07-17 00:00:00
abstract:AIMS:Tetramethylpyrazine (TMP), one of the active ingredients isolated from a Chinese herbal prescription, possesses protective effects against oxidative stress caused by high glucose in endothelial cells. In this study, the role of TMP in preventing muscle cells from palmitate-induced oxidative damage was investigated...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2011.02.025
更新日期:2011-04-25 00:00:00
abstract:AIMS:Solid lipid nanoparticles (SLNs) conjugated with surface 83-14 monoclonal antibody (8314MAb) and anti-epithelial growth factor receptor (AEGFR) were synthesized and applied to permeate the blood-brain barrier (BBB) and inhibit the growth of glioblastoma multiforme (GBM). MAIN METHODS:8314MAb and AEGFR were crossl...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2016.01.025
更新日期:2016-02-01 00:00:00
abstract::Autophagy is a metabolic process that maintains the homeostasis of the intracellular environment by degrading damaged proteins and organelles in lysosomes. Recently, independent studies have indicated that some hepatitis viruses develop abilities to hijack autophagy for their replication, such as hepatitis B (HBV) and...
journal_title:Life sciences
pub_type: 杂志文章,评审
doi:10.1016/j.lfs.2019.117206
更新日期:2020-02-01 00:00:00