当前位置： SCI文献检索 > HUMAN PATHOLOGY期刊下所有文献 > Molecular analysis of primary gastric cancer, corresponding xenografts, and 2 novel gastric carcinoma cell lines reveals novel alterations in gastric carcinogenesis.

Molecular analysis of primary gastric cancer, corresponding xenografts, and 2 novel gastric carcinoma cell lines reveals novel alterations in gastric carcinogenesis.

Abstract：

:We report the molecular characterization of 8 primary gastric carcinomas, corresponding xenografts, and 2 novel gastric carcinoma cell lines. We compared the tumors and cell lines, with respect to histology, immunohistochemistry, copy number, and hypermethylation of up to 38 genes using methylation-specific multiplex ligation-dependent probe amplification, and TP53 and CDH1 mutation analysis where relevant. The primary tumors and xenografts were histologically comparable and shared expression of 11 of 14 immunohistochemical markers (E-cadherin, beta-catenin, COX-2, p53, p16, TFF1, cyclin E, MLH1, SMAD4, p27, KLK3, CASR, CHFR, and DAPK1). Gains of CASR, DAPK1, and KLK3--not yet described in gastric cancer--were present in the primary tumors, xenografts, and cell lines. The most prominent losses occurred at CDKN2A (p16), CDKN2B (p15), CDKN1B (p27/KIP1), and ATM. Except for ATM, these losses were found only in the cell line or xenograft, suggesting an association with tumor progression. However, examination of p16 and p27 in 174 gastric cancers using tissue microarrays revealed no significant correlation with tumor stage or lymph node status. Further losses and hypermethylation were detected for MLH1, CHFR, RASSF1, and ESR, and were also seen in primary tumors. Loss of CHFR expression correlated significantly with the diffuse phenotype. Interestingly, we found the highest rate of methylation in primary tumors which gave rise to cell lines. In addition, both cell lines harbored mutations in CDH1, encoding E-cadherin. Xenografts and gastric cancer cell lines remain an invaluable research tool in the uncovering of the multistep progression of cancer. The frequent gains, losses, and hypermethylation reported in this study indicate that the involved genes or chromosomal regions may be relevant to gastric carcinogenesis.

journal_name

Hum Pathol

journal_title

Human pathology

authors

Milne AN,Sitarz R,Carvalho R,Polak MM,Ligtenberg M,Pauwels P,Offerhaus GJ,Weterman MA

doi

10.1016/j.humpath.2006.12.010

subject

Has Abstract

pub_date

2007-06-01 00:00:00

pages

903-13

issue

6

eissn

0046-8177

issn

1532-8392

pii

S0046-8177(06)00734-9

journal_volume

38

pub_type

杂志文章

相关文献

HUMAN PATHOLOGY文献大全
  • The molecular biology of esophageal and gastric cancer and their precursors: oncogenes, tumor suppressor genes, and growth factors.

    abstract：:The evolution of sequential histological changes from normal cells through invasive cancer affords the cancer biologist the opportunity to identify separate molecular steps involved in cancer progression. As one studies the development of human carcinoma, it becomes apparent that multiple genetic alterations affecting...

    journal_title：Human pathology

    pub_type： 杂志文章,评审

    doi：10.1016/0046-8177(94)90056-6

    authors： Stemmermann G,Heffelfinger SC,Noffsinger A,Hui YZ,Miller MA,Fenoglio-Preiser CM

    更新日期：1994-10-01 00:00:00

  • Applications of electron microscopy to diagnostic pulmonary pathology.

    abstract：:Viruses and other possible causative agents should be sought light and electron microscopically in all cases of ill-defined diseases including "sarcoid." Ideally, tissue should be prepared for electron microscopic examination as soon as a specimen is obtained; however, when this has not been done, tissue preserved in ...

    journal_title：Human pathology

    pub_type： 杂志文章,评审

    doi：10.1016/s0046-8177(83)80164-6

    authors： Wang NS

    更新日期：1983-10-01 00:00:00

  • Pathology trainee manpower: APC program directors' questionnaire, 1989 results.

    abstract：:The shortage of pathologists is being created by a combination of three factors: inadequate trainee recruitment, high trainee attrition, and accelerating practitioner attrition. The current report provides comprehensive data on the first two factors. The third factor is, by every estimate, likely to become worse over ...

    journal_title：Human pathology

    pub_type： 杂志文章

    doi：10.1016/0046-8177(91)90258-q

    authors： Vance RP,Prichard RW,Smith RD

    更新日期：1991-11-01 00:00:00

  • Testicular atrophy in AIDS: a study of 57 autopsy cases.

    abstract：:The pertinent clinical data and histologic features of the testes in 57 autopsied acquired immunodeficiency syndrome (AIDS) patients were analyzed and compared with those of 55 age-matched control patients without AIDS. The testes of the AIDS patients showed a significantly lower degree of spermatogenesis (determined ...

    journal_title：Human pathology

    pub_type： 杂志文章

    doi：10.1016/0046-8177(89)90125-1

    authors： De Paepe ME,Waxman M

    更新日期：1989-03-01 00:00:00

  • Expression and prognostic significance of matrix metalloproteinases and their tissue inhibitors in primary neuroendocrine carcinoma of the skin.

    abstract：:Matrix metalloproteinases (MMPs) and their tissue inhibitors (TIMPs) have been implicated in the development and progression of many tumors, but data for primary neuroendocrine carcinoma (PNC) of the skin are lacking. The aim of the study was to assess the expression of MMPs and TIMPs in PNC and to evaluate their prog...

    journal_title：Human pathology

    pub_type： 杂志文章

    doi：10.1053/hupa.2003.51

    authors： Massi D,Franchi A,Ketabchi S,Paglierani M,Pimpinelli N,Santucci M

    更新日期：2003-01-01 00:00:00

  • Use of remote video microscopy (telepathology) as an adjunct to neurosurgical frozen section consultation.

    abstract：:We investigated the use of remote video microscopy (telepathology) to assist in the diagnosis of 52 neurosurgical frozen section cases. The TelMed system (Discovery Medical Systems, Overland Park, KS), in which the referring pathologist selects appropriate fields for transmission to the consultant, was used for the st...

    journal_title：Human pathology

    pub_type： 杂志文章

    doi：10.1016/0046-8177(93)90142-4

    authors： Becker RL Jr,Specht CS,Jones R,Rueda-Pedraza ME,O'Leary TJ

    更新日期：1993-08-01 00:00:00

  • Mixed epithelial and stromal tumor of the middle ear: The first case report.

    abstract：:We report a tumor arising in the middle ear of a 65-year-old female patient that was composed of an ovarian-type stroma (OS) and an epithelial component. The tumor consisted of irregular, polypoid masses containing multiple variably sized cystic spaces, which were invariably surrounded by the OS. The cystic spaces wer...

    journal_title：Human pathology

    pub_type： 杂志文章

    doi：10.1016/j.humpath.2016.09.020

    authors： Michal M,Skálová A,Kazakov DV,Pecková K,Heidenreich F,Grossmann P,Michal M

    更新日期：2017-03-01 00:00:00

  • Isolated xanthomatosis of the small bowel.

    abstract：:A case of xanthomatosis of the small bowel is described. Beginning with the proximal jejunum, an 85-cm segment of small bowel was distorted by regularly spaced nodular accumulations of lipid-laden macrophages that expanded the submucosa and muscularis and extended to the serosal surface. The conditions often associate...

    journal_title：Human pathology

    pub_type： 杂志文章

    doi：10.1016/s0046-8177(85)80237-9

    authors： Coletta U,Sturgill BC

    更新日期：1985-04-01 00:00:00

  • Profiles of chemokine receptors in melanocytic lesions: de novo expression of CXCR6 in melanoma.

    abstract：:Selective expression of certain chemokine receptors by melanoma cells and the presence of their ligands in tissues might govern organ site-specific metastasis. Because the expression profile of chemokine receptors in tissues of melanocytic origin is unknown, we performed a comprehensive study on melanocytic tissue sam...

    journal_title：Human pathology

    pub_type： 杂志文章

    doi：10.1016/j.humpath.2006.11.013

    authors： Seidl H,Richtig E,Tilz H,Stefan M,Schmidbauer U,Asslaber M,Zatloukal K,Herlyn M,Schaider H

    更新日期：2007-05-01 00:00:00

  • The utility of IgM, CD21, HGAL and LMO2 in the diagnosis of pediatric follicular lymphoma.

    abstract：:Pediatric follicular lymphoma (pFL) is a rare neoplasm with features differing from follicular lymphoma arising in adults. Here, we describe a rare case of pFL that showed morphologic features partially overlapping with progressive transformation of germinal centers and reactive follicular hyperplasia. As typical of p...

    journal_title：Human pathology

    pub_type： 杂志文章

    doi：10.1016/j.humpath.2014.12.016

    authors： Karnik T,Ozawa MG,Lefterova M,Luna-Fineman S,Alvarez E,Link M,Zehnder JL,Arber DA,Ohgami RS

    更新日期：2015-04-01 00:00:00

  • Expression of Bfl-1 in normal and tumor tissues: Bfl-1 overexpression in cancer is attributable to its preferential expression in infiltrating inflammatory cells.

    abstract：:Bfl-1, a member of the Bcl-2 gene family, blocks p53-mediated apoptosis and has oncogenic transforming activity. In normal tissues, the transcript of Bfl-1 is expressed abundantly in bone marrow and at a low level in several other tissues. In previous experiments, elevated expression of Bfl-1 was observed by Northern ...

    journal_title：Human pathology

    pub_type： 杂志文章

    doi：10.1016/s0046-8177(98)90282-9

    authors： Jung-Ha H,Kim D,Lee SB,Hong SI,Park SY,Huh J,Kim CW,Kim SS,Lee Y,Choi SS,Shin HS

    更新日期：1998-07-01 00:00:00

  • Silicone lymphadenopathy: a complication of silicone elastomer finger joint prostheses.

    abstract：:Two cases of lymphadenopathy resulting from a lymph node foreign body reaction to silicone are presented. In each case lymph node biopsy was performed because of the clinical suspicion of neoplastic disease. Histologic study in both cases revealed no evidence of malignant disease, but rather demonstrated multinucleate...

    journal_title：Human pathology

    pub_type： 杂志文章

    doi：10.1016/s0046-8177(80)80005-0

    authors： Kircher T

    更新日期：1980-05-01 00:00:00

  • Expression of epidermal growth factor receptor, but not K-RAS mutations, is present in congenital cystic airway malformation/congenital pulmonary airway malformation.

    abstract：:Congenital cystic airway malformation/congenital pulmonary airway malformation (CCAM/CPAM) of the lung is a rare but well-described malformative lesion of pulmonary parenchyma characterized by the abnormal maturation of airways along with an increase in terminal respiratory structures, resulting in cysts of variable s...

    journal_title：Human pathology

    pub_type： 杂志文章

    doi：10.1016/j.humpath.2007.04.009

    authors： Guo H,Cajaiba MM,Borys D,Gutierrez MC,Yee H,Drut RM,Drut R,Askin F,Reyes-Múgica M,Greco MA

    更新日期：2007-12-01 00:00:00

  • Thyroid gland papillary carcinomas with "micropapillary pattern," a recently recognized poor prognostic finding: clinicopathologic and survival analysis of 7 cases.

    abstract：:Micropapillary carcinoma is a histologic pattern, rather than an independent entity, that has an aggressive clinical behavior regardless of location. Histologically, it is characterized by papillary cell groups in clear spaces. The micropapillary pattern in the thyroid gland has not been studied until recently but und...

    journal_title：Human pathology

    pub_type： 杂志文章

    doi：10.1016/j.humpath.2011.10.027

    authors： Lino-Silva LS,Domínguez-Malagón HR,Caro-Sánchez CH,Salcedo-Hernández RA

    更新日期：2012-10-01 00:00:00

  • Integrated multimodal genetic testing of Ewing sarcoma--a single-institution experience.

    abstract：:Ewing sarcoma (ES) is an aggressive malignant small round cell tumor that arises in bone or soft tissue of adolescents and young adults. A characteristic molecular finding in ES is EWSR1 gene fusion with ETS (erythroblast transformation-specific) family genes including FLI1 (~90%) and ERG (>5%). Here we report our exp...

    journal_title：Human pathology

    pub_type： 杂志文章

    doi：10.1016/j.humpath.2013.03.003

    authors： Warren M,Weindel M,Ringrose J,Venable C,Reyes A,Terashima K,Rao P,Chintagumpala M,Hicks MJ,Lopez-Terrada D,Lu XY

    更新日期：2013-10-01 00:00:00

  • Mineral oil (follicular) lipidosis: II. Histologic studies of spleen, liver, lymph nodes, and bone marrow.

    abstract：:Tissues obtained from 600 routine autopsies were studied. Mineral oil lipidosis was present in the spleen (76 per cent), liver (45 per cent), bone marrow (26 per cent), and lymph nodes; more than 50 per cent of the lymph nodes from the mesentery, porta hepatis, and mediastinum were affected. Mineral oil and its metabo...

    journal_title：Human pathology

    pub_type： 杂志文章

    doi：10.1016/s0046-8177(84)80163-x

    authors： Cruickshank B,Thomas MJ

    更新日期：1984-08-01 00:00:00

  • Angiotropic large-cell lymphoma presenting as pulmonary small vessel occlusive disease.

    abstract：:Angiotropic lymphoma (AL) is an unusual variant of extranodal lymphoma, characterized by massive proliferation of neoplastic lymphoid cells almost exclusively within blood vessels. Whereas the lymphoid origin of this disease is widely accepted it still remains unclear whether AL is a distinct entity that originates in...

    journal_title：Human pathology

    pub_type： 杂志文章

    doi：10.1053/hupa.2000.7636

    authors： Evert M,Lehringer-Polzin M,Möbius W,Pfeifer U

    更新日期：2000-07-01 00:00:00

  • Basal cell hyperplasia, adenoid basal cell tumor, and adenoid cystic carcinoma of the prostate gland: an immunohistochemical study.

    abstract：:Basal cell hyperplasia (BCH) is an uncommon proliferative lesion of the prostate gland. We studied ten cases of BCH, one case of an unusual adenoid basal cell tumor (ABT), and one case of a prostatic adenoid cystic carcinoma (ACC), using a panel of antibodies to define the histogenesis of these lesions. Monoclonal ant...

    journal_title：Human pathology

    pub_type： 杂志文章

    doi：10.1016/s0046-8177(88)80235-1

    authors： Grignon DJ,Ro JY,Ordoñez NG,Ayala AG,Cleary KR

    更新日期：1988-12-01 00:00:00

  • Diagnosis of chronic endometritis in biopsies with stromal breakdown.

    abstract：:Plasma cells are the hallmark of chronic endometritis but are not specific for upper tract infection. Plasma cells have also been noted in hormonally mediated endometrial disorders in association with gland architectural changes ("disordered proliferative" and "anovulatory" patterns), and stromal breakdown. We reviewe...

    journal_title：Human pathology

    pub_type： 杂志文章

    doi：10.1016/j.humpath.2006.09.002

    authors： Gilmore H,Fleischhacker D,Hecht JL

    更新日期：2007-04-01 00:00:00

  • Veno-occlusive disease of the liver associated with oral contraceptives: case report and review of literature.

    abstract：:This report concerns a young woman who, after taking a contraceptive preparation orally for three years, developed the Budd-Chiari syndrome as the result of a widespread chronic obliterative process involving the intrahepatic efferent venous system. Her prolonged course, which failed to respond to an end-to-side porta...

    journal_title：Human pathology

    pub_type： 杂志文章

    doi：10.1016/s0046-8177(76)80082-2

    authors： Alpert LI

    更新日期：1976-11-01 00:00:00

  • Epidermal growth factor receptor in glioblastomas: correlation between gene copy number and protein expression.

    abstract：:Epidermal growth factor receptor is a transmembrane receptor involved in oncogenesis, including the development of glioblastoma. We studied the prognostic significance of epidermal growth factor receptor amplification as determined by fluorescence in situ hybridization, quantitative polymerase chain reaction, and prot...

    journal_title：Human pathology

    pub_type： 杂志文章

    doi：10.1016/j.humpath.2009.09.020

    authors： Coulibaly B,Nanni I,Quilichini B,Gaudart J,Metellus P,Fina F,Boucard C,Chinot O,Ouafik L,Figarella-Branger D

    更新日期：2010-06-01 00:00:00

  • Computerized microscope morphometry of umbilical vessels from pregnancies with intrauterine growth retardation and abnormal umbilical artery Doppler.

    abstract：:Computerized microscope morphometry was used to study cross sections from the vessels of the umbilical cord in placentas of patients with intrauterine growth retardation (IUGR) that displayed either normal or abnormal umbilical arteries (UA) Doppler flow velocity waveforms (FVW). Cords from 63 eutrophic fetuses with n...

    journal_title：Human pathology

    pub_type： 杂志文章

    doi：10.1016/s0046-8177(97)90251-3

    authors： Bruch JF,Sibony O,Benali K,Challier JC,Blot P,Nessmann C

    更新日期：1997-10-01 00:00:00

  • Primary (granulomatous) angiitis of the central nervous system: a clinicopathologic analysis of 15 new cases and a review of the literature.

    abstract：:The clinical and pathologic features of 15 new cases of the uncommon primary or granulomatous angiitis of the central nervous system (PACNS) are described. To date, only 108 such cases have been reported in the English literature. Clinically, most PACNS patients have been young or middle-aged (mean age, 45 years; rang...

    journal_title：Human pathology

    pub_type： 杂志文章,评审

    doi：10.1016/0046-8177(92)90238-x

    authors： Lie JT

    更新日期：1992-02-01 00:00:00

  • Histone 3.3 mutations in giant cell tumor and giant cell-rich sarcomas of bone.

    abstract：:Mutually exclusive histone 3.3 gene mutations have been recognized in chondroblastoma and giant cell tumor of bone (GCTB), which may be useful for differential diagnostic purposes in morphologically ambiguous cases. Although more than 90% of GCTBs present histone 3.3 variants exclusively in the H3F3A gene, chondroblas...

    journal_title：Human pathology

    pub_type： 杂志文章

    doi：10.1016/j.humpath.2017.08.033

    authors： Righi A,Mancini I,Gambarotti M,Picci P,Gamberi G,Marraccini C,Dei Tos AP,Simi L,Pinzani P,Franchi A

    更新日期：2017-10-01 00:00:00

  • Phospho-STAT5 expression pattern with the MPL W515L mutation is similar to that seen in chronic myeloproliferative disorders with JAK2 V617F.

    abstract：:Abnormal nuclear megakaryocytic staining for phospho-STAT5 (pSTAT5) correlates with JAK2 V617F mutational status in non-chronic myelogenous leukemia chronic myeloproliferative disorders. However, a proportion of wild-type JAK2 non-chronic myelogenous leukemia chronic myeloproliferative disorders cases also demonstrate...

    journal_title：Human pathology

    pub_type： 杂志文章

    doi：10.1016/j.humpath.2007.10.034

    authors： Gibson SE,Schade AE,Szpurka H,Bak B,Maciejewski JP,Hsi ED

    更新日期：2008-07-01 00:00:00

  • TGF beta 1 and TGF beta 2 are potential growth regulators for medulloblastomas, primitive neuroectodermal tumors, and ependymomas: evidence in support of an autocrine hypothesis.

    abstract：:Our previous investigations of transforming growth factor types beta 1 and beta 2 (TGF beta s) showed negative or positive autocrine growth regulation of gliomas in vitro. Near-diploid gliomas were inhibited by the TGF beta s, whereas a stimulatory response correlated with progressive anaplasia and karyotypic divergen...

    journal_title：Human pathology

    pub_type： 杂志文章

    doi：10.1016/0046-8177(94)90118-x

    authors： Jennings MT,Kaariainen IT,Gold L,Maciunas RJ,Commers PA

    更新日期：1994-05-01 00:00:00

  • Microtubular aggregates within rough endoplasmic reticulum: an unusual ultrastructural feature of extraskeletal myxoid chondrosarcoma.

    abstract：:Two extraskeletal myxoid chondrosarcomas were examined by electron microscopy. In addition to the well-described ultrastructural features indicative of chondroblastic origin, both of these chondrosarcomas had unusual parallel microtubules packed within rough endoplasmic reticulum. This paper reviews the differential d...

    journal_title：Human pathology

    pub_type： 杂志文章

    doi：10.1016/s0046-8177(86)80036-3

    authors： DeBlois G,Wang S,Kay S

    更新日期：1986-05-01 00:00:00

  • Significance of topoisomerase IIIβ expression in breast ductal carcinomas: strong associations with disease-specific survival and metastasis.

    abstract：:Topoisomerases are ubiquitous nuclear enzymes that regulate DNA structure in eukaryotic cells. The role of topoisomerase IIIβ, the newest member of the topoisomerase family, in the clinical outcome of breast cancer is still poorly understood. This study aims to investigate the immunoexpression of topoisomerase IIIβ in...

    journal_title：Human pathology

    pub_type： 杂志文章

    doi：10.1016/j.humpath.2010.01.027

    authors： Oliveira-Costa JP,Zanetti J,Oliveira LR,Soares FA,Ramalho LZ,Silva Ramalho F,Garcia SB,Ribeiro-Silva A

    更新日期：2010-11-01 00:00:00

  • Using p16 immunohistochemistry to classify morphologic cervical intraepithelial neoplasia 2: correlation of ambiguous staining patterns with HPV subtypes and clinical outcome.

    abstract：:p16INK4a immunohistochemistry (IHC) is widely used to facilitate the diagnosis of human papillomavirus (HPV)-associated cervical precancerous lesions. Although most p16 results are distinctly positive or negative, certain ones are ambiguous: they meet some but not all requirements for the "block-positive" pattern. It ...

    journal_title：Human pathology

    pub_type： 杂志文章

    doi：10.1016/j.humpath.2017.06.014

    authors： Liu Y,Alqatari M,Sultan K,Ye F,Gao D,Sigel K,Zhang D,Kalir T

    更新日期：2017-08-01 00:00:00

  • The value of monoclonal antibody B72.3 for the diagnosis of breast carcinoma: experience with the first commercially available source.

    abstract：:One hundred cases of invasive breast carcinoma were studied using the commercially available monoclonal antibody Anti-Human Tumor-Associated Glycoprotein-72 (MAb B72.3, Biomedical Technologies Inc, Stoughton, MA) prediluted at 8.5 micrograms/mL. Forty-three cases displayed positive reactivity with this antibody. Inten...

    journal_title：Human pathology

    pub_type： 杂志文章

    doi：10.1016/0046-8177(91)90238-k

    authors： Prey MU,Bedrossian CW,Masood S

    更新日期：1991-06-01 00:00:00