Abstract:
:Epidermal growth factor receptor is a transmembrane receptor involved in oncogenesis, including the development of glioblastoma. We studied the prognostic significance of epidermal growth factor receptor amplification as determined by fluorescence in situ hybridization, quantitative polymerase chain reaction, and protein expression by immunohistochemistry. Ninety-nine patients exhibiting glioblastoma were included. Immunohistochemistry was performed on microarray blocks with clone 25, which recognizes both epidermal growth factor receptor wild type and vIII, and scored using a semiquantitative approach. Quantitative polymerase chain reaction and fluorescence in situ hybridization techniques were performed on frozen section: 29.3% of cases had a high epidermal growth factor receptor immunohistochemistry score (score >/=200); and of these cases, 96.5% had gene amplification by fluorescence in situ hybridization and quantitative polymerase chain reaction. Conversely, of cases with a low immunohistochemistry score, 72.9% had normal karyotype or polysomy 7 by fluorescence in situ hybridization technique; but around 25% had gene amplification by fluorescence in situ hybridization and quantitative polymerase chain reaction. In the case of protein overexpression, quantitative polymerase chain reaction and fluorescence in situ hybridization could be avoided in first intention because their positive predictive value for amplification is 97%. In multivariate analysis, there was a trend toward an association between shorter overall survival time and epidermal growth factor receptor amplification as determined by fluorescence in situ hybridization analysis. However, cases with an immunohistochemistry score less 200 require further testing by fluorescence in situ hybridization or quantitative polymerase chain reaction.
journal_name
Hum Patholjournal_title
Human pathologyauthors
Coulibaly B,Nanni I,Quilichini B,Gaudart J,Metellus P,Fina F,Boucard C,Chinot O,Ouafik L,Figarella-Branger Ddoi
10.1016/j.humpath.2009.09.020subject
Has Abstractpub_date
2010-06-01 00:00:00pages
815-23issue
6eissn
0046-8177issn
1532-8392pii
S0046-8177(09)00462-6journal_volume
41pub_type
杂志文章相关文献
HUMAN PATHOLOGY文献大全abstract::Pancreatic cancer (PaCA) is a deadly disease with few systemic therapeutic options. The head of the pancreas is the most innervated part and most common location of cancer. However, little is known about the contribution of the nerve-cancer interaction to facilitate pancreatic progression. To quantify PaCA axonogenesi...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/j.humpath.2016.02.001
更新日期:2016-06-01 00:00:00
abstract::The diagnosis of placental site trophoblastic lesions, particularly the distinction of a placental site trophoblastic tumor from an exaggerated placental site, can be difficult. Mel-CAM (also known as CD146 and MUC18) is a recently recognized cell adhesion molecule belonging to the immunoglobin gene superfamily that s...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/s0046-8177(98)90386-0
更新日期:1998-01-01 00:00:00
abstract::Previous immunohistochemical (IHC) studies showed controversial data about the prognostic value of tumor-infiltrating lymphocytes (TILs) in follicular lymphoma (FL). To clarify this issue, a large series of FL samples from rituximab-treated patients enrolled in the randomized PRIMA trial was examined. IHC was quantifi...
journal_title:Human pathology
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1016/j.humpath.2017.03.023
更新日期:2017-06-01 00:00:00
abstract::Extramammary Paget disease (EMPD) has been known to frequently express androgen receptor (AR). Therefore, androgens could play roles in the biological behavior of Paget cells. 5α-Reductase (5α-red) types 1 and 2 and 17β-hydroxysteroid dehydrogenase type 5 (17β-HSD5) are pivotal in situ regulators of androgen productio...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/j.humpath.2015.07.007
更新日期:2015-11-01 00:00:00
abstract::δ-Like ligand 4 (DLL4), a ligand for the Notch family of receptors, forecasts the prognosis of several human malignancies. However, the expression and role of DLL4 in breast cancer remain largely unknown. In the present study, we first evaluated whether the overexpression of DLL4 could be used as an indicator of axill...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/j.humpath.2014.04.025
更新日期:2014-11-01 00:00:00
abstract::Four cases of radiation cystitis that caused diagnostic difficulty because of an epithelial proliferation with architectural complexity and reactive cytologic atypia are described. The patients, 2 male, 2 female, were from 43 to 77 years of age. Two presented with hematuria. Cystoscopy disclosed abnormalities in 3 pat...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1053/hupa.2000.7894
更新日期:2000-06-01 00:00:00
abstract::Astrocytoma grade IV (glioblastoma multiforme) is the most common and most malignant tumor of the central nervous system and is currently noncurable. Here, we have examined a population-based cohort of 47 patients with grade IV astrocytoma, who underwent tumor surgery at Sahlgrenska University Hospital in Sweden and w...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/j.humpath.2013.03.013
更新日期:2013-10-01 00:00:00
abstract::The evolution of sequential histological changes from normal cells through invasive cancer affords the cancer biologist the opportunity to identify separate molecular steps involved in cancer progression. As one studies the development of human carcinoma, it becomes apparent that multiple genetic alterations affecting...
journal_title:Human pathology
pub_type: 杂志文章,评审
doi:10.1016/0046-8177(94)90056-6
更新日期:1994-10-01 00:00:00
abstract::Basaloid carcinoma (BC) and large-cell neuroendocrine carcinoma (LCNEC) are 2 recently recognized variants of large-cell lung carcinomas that may overlap in their morphology, and are discriminated by expression of neuroendocrine markers in LCNEC. Because thyroid transcription factor 1 (TTF-1) is expressed in lung aden...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1053/hupa.2001.27110
更新日期:2001-09-01 00:00:00
abstract::Pseudomyogenic hemangioendothelioma (PHE) is an uncommon neoplasm with propensity for local recurrence. The tumor mimics epithelioid hemangioendothelioma and epithelioid sarcoma, representing a possible diagnostic pitfall. We investigated the clinicopathological, immunohistochemical, and fluorescence in situ hybridiza...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/j.humpath.2017.10.023
更新日期:2018-01-01 00:00:00
abstract::Automatic quantification of cardinal histologic features of nonalcoholic fatty liver disease (NAFLD) may reduce human variability and allow continuous rather than semiquantitative assessment of injury. We recently developed an automated classifier that can detect and quantify macrosteatosis with greater than or equal ...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/j.humpath.2015.01.019
更新日期:2015-05-01 00:00:00
abstract::A distinctive ultrastructural finding, observed in a case of acute lymphoblastic leukemia (ALL) and previously unreported on the surface of bone marrow cells, is described. The alteration is referred to as distinctive cytoplasmic surface activity (DCSA). For further delineation, study of 130 consecutively submitted bo...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/s0046-8177(87)80166-1
更新日期:1987-04-01 00:00:00
abstract::The expression/activation of epidermal growth factor receptor (EGFR) and the correlation with the phosphorylation status of downstream modulator proteins, Akt, mammalian target of rapamycin (mTOR), p70S6-kinase (S6K), ribosomal protein S6 (rS6), and eukaryotic initiation factor 4E-binding protein 1 (4E-BP1), were anal...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/j.humpath.2010.05.025
更新日期:2011-02-01 00:00:00
abstract::Phyllodes tumors are rare but clinically important fibroepithelial tumors of the breast. Both epithelial and stromal components actively interact with each other to participate in phyllodes tumor development. Accumulated evidence suggests that the Wnt signaling pathway is important in this stromal-epithelial interacti...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/j.humpath.2012.03.028
更新日期:2012-12-01 00:00:00
abstract::Hepatitis C virus (HCV) infection may be complicated by non-Hodgkin's lymphoma through yet unknown pathogenetic mechanisms. We describe the case of a patient with HCV-related cirrhosis who developed a primary effusion lymphoma (PEL) of Burkitt's type confined to the peritoneal cavity, in the absence of immunodeficienc...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/s0046-8177(97)90287-2
更新日期:1997-01-01 00:00:00
abstract::In this series of 3,432 cases of malignant melanoma of the choroid and ciliary body, mortality from metastasis 15 years after enucleation was 46 per cent. This mortality was at least ten times greater than has been observed with tumors of the iris, probably owing to the greater size and more malignant cytology of chor...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/s0046-8177(82)80116-0
更新日期:1982-02-01 00:00:00
abstract::Several techniques for diagnosing adenocarcinoma of the lung are commonly available, but the frequency of their use and diagnostic sensitivity may vary. Twenty cases of primary lung adenocarcinoma obtained at surgery were studied by the following four routine techniques: light microscopy (LM) using hematoxylin-eosin (...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/s0046-8177(88)80005-4
更新日期:1988-08-01 00:00:00
abstract::A study of so-called gelatinous transformation of the bone marrow in 14 patients is presented. The condition is characterized by the presence of extracellular "gelatinous" material, fat atrophy, and associated focal marrow hypoplasia and is often mistaken as representing edema, necrosis, or amyloid. Histochemical stud...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/s0046-8177(78)80051-3
更新日期:1978-11-01 00:00:00
abstract::The aim of the study was to evaluate the impact of the updated 2018 American Society of Clinical Oncology (ASCO)/College of American Pathologists (CAP) guideline on human epidermal growth factor receptor 2 (HER2) interpretation in breast cancer compared with that of the previous guidelines and also the significance of...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/j.humpath.2020.01.003
更新日期:2020-04-01 00:00:00
abstract::Peritoneal involvement in colorectal cancer (CRC) has prognostic significance and is an important parameter in pathologic tumor staging. Restaging of tumors based on peritoneal elastic lamina invasion (ELI) has prognostic significance in CRCs classified as pathologic stage 3 tumors without regional lymph node metastas...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/j.humpath.2018.10.019
更新日期:2019-03-01 00:00:00
abstract::Fifty-three surgically resected small cell carcinomas of the lung were studied morphometrically, electron microscopically, immunohistochemically, and in terms of possible site of origin. Four subtypes of small cell carcinomas were identified: oat cell carcinoma (OAT), small cell carcinoma of the intermediate cell type...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/s0046-8177(86)80133-2
更新日期:1986-06-01 00:00:00
abstract::The very late antigens (VLAs) are alpha beta-heterodimeric transmembrane proteins that include surface cell receptors for laminin (VLA-6) and collagen (VLA-2), which mediate cell-matrix and cell-cell adhesion. We investigated the distribution of VLA-6 (alpha 6, beta 1) and VLA-2 (alpha 2, beta 1) proteins in normal, h...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/0046-8177(93)90033-d
更新日期:1993-03-01 00:00:00
abstract::In two patients with malignant germ cell tumors angiosarcoma developed through two apparently different mechanisms. In one case the angiosarcoma probably developed as a complication of therapeutic radiation, since radiation changes were demonstrated in tissue adjacent to the neoplasm and since the angiosarcoma was not...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/s0046-8177(85)80013-7
更新日期:1985-03-01 00:00:00
abstract::A renal biopsy of a 57-year-old man with nephrotic syndrome revealed marked glomerular deposition of Congo red-positive, bire-fringent material characteristic for amyloid. Ultrastructurally, however, this material is composed of nonbranching, haphazardly arranged fibrils of approximately three times the thickness of t...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/0046-8177(90)90059-e
更新日期:1990-08-01 00:00:00
abstract::Recently, a polymorphism in the hMSH2 DNA mismatch repair gene has been associated with the development of dysplasia in ulcerative colitis (UC) patients. This polymorphism is of interest because DNA mismatch repair defects result in alterations in microsatellite stability. The current study was designed to determine w...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/s0046-8177(99)90293-9
更新日期:1999-01-01 00:00:00
abstract::We report two cases of allergic bronchopulmonary fungal disease (ABPFD) caused by Curvularia sp and associated with allergic fungal sinusitis (AFS). Curvularia lunata was cultured in one case and Curvularia senegalensis was cultured in the other. Based on these cases and a review of the literature, we discuss unusual ...
journal_title:Human pathology
pub_type: 杂志文章,评审
doi:10.1016/0046-8177(91)90106-y
更新日期:1991-12-01 00:00:00
abstract::Cholangiocarcinoma of intrahepatic and extrahepatic bile ducts has a multistep carcinogenesis. Two premalignant lesions have been suggested for invasive cholangiocarcinoma: biliary intraepithelial neoplasia and intraductal papillary neoplasm of the bile duct. How the carcinogenetic process differs between biliary intr...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/j.humpath.2007.11.018
更新日期:2008-08-01 00:00:00
abstract::Of the approximately 635 residents in each year of pathology training, about 165 are already meeting the Pathology Board's proposed requirement for a fifth year of resident training. The cost of a fifth year of training for the other 470 residents is estimated to be approximately $20 million. Options for avoiding this...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/s0046-8177(84)80159-8
更新日期:1984-08-01 00:00:00
abstract::Most human papillomavirus-associated squamous intraepithelial lesions of the uterine cervix are flat; some have papillary architecture that shows a spectrum of differentiation from low-grade squamous intraepithelial lesions to high-grade squamous intraepithelial lesions. For this subset of lesions, there are few data ...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/j.humpath.2009.05.015
更新日期:2010-03-01 00:00:00
abstract::Twenty-six women with multiple human papillomavirus (HPV)-related lesions of the lower genital tract were investigated by immunohistochemistry for the internal genus-specific capsid antigen of HPV and by DNA-DNA in situ hybridization with 35S-radiolabeled probes for sequences of HPV-6/11, HPV-16, and HPV-18. The vulva...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/0046-8177(89)90175-5
更新日期:1989-02-01 00:00:00