Abstract：
:Pancreatic cancer (PaCA) is a deadly disease with few systemic therapeutic options. The head of the pancreas is the most innervated part and most common location of cancer. However, little is known about the contribution of the nerve-cancer interaction to facilitate pancreatic progression. To quantify PaCA axonogenesis, we used a 3-dimensional in vitro neurogenesis model. In addition, neurogenesis in human PaCA was analyzed using PGP9.5 immunohistochemistry, deconvolution imaging, and image segmentation and analysis. There was a significant increase of the total area of neurites in the in vitro coculture with dorsal root ganglia group than control. The nerve density in PaCA tissue was significantly higher than normal pancreatic tissue. To study the functional role of nerves in PaCA, male athymic nude (Nu-Nu) mice were divided into 3 groups: (A) animals were coinjected with MIA PaCa-2 cells and 20U/kg weight units of Botulinum toxin (Botox) (n=10); (B) first injected with Botox and 6weeks later MIA PaCa-2 cancer cells (n=4); and (C) control animals were injected with equivalent amounts of saline fluid (n=9). Animals were sacrificed 6weeks later. Tumor size and apoptotic count (terminal deoxynucleotidyl transferase dUTP nick-end labeling) were measured. Tumor size was decreased and apoptotic rate increased in Botox-treated PaCA. Our data indicate that neural microenvironment may play an important role in the progression of PaCA. It may lead to novel nerve-targeted coadjuvant therapies for PaCA.
journal_name
Hum Patholjournal_title
Human pathologyauthors
He D,Manzoni A,Florentin D,Fisher W,Ding Y,Lee M,Ayala Gdoi
10.1016/j.humpath.2016.02.001subject
Has Abstractpub_date
2016-06-01 00:00:00pages
182-9eissn
0046-8177issn
1532-8392pii
S0046-8177(16)00063-0journal_volume
52pub_type
杂志文章相关文献
HUMAN PATHOLOGY文献大全abstract：:Chemokine receptor, CXCR3, has been increasingly reported to be involved in tumorigenesis and tumor progression, but limited data are available regarding the expression of CXCR3 in gastric cancer (GC). In the present study, the expressions of CXCR3 and its variants were detected in 96 GC and corresponding nontumor gas...
journal_title：Human pathology
pub_type： 杂志文章
doi：10.1016/j.humpath.2015.08.004
更新日期：2015-12-01 00:00:00
abstract：:The presence of thyroglobulin (TG) was investigated by immunohistochemistry with one polyclonal and three distinct monoclonal anti-TG antibodies in 19 primary and 15 metastatic medullary thyroid carcinoma (MTC) cases. In two primary tumors possessing a mixed trabecular and follicular growth pattern, a significant numb...
journal_title：Human pathology
pub_type： 杂志文章
doi：10.1016/0046-8177(93)90035-f
更新日期：1993-03-01 00:00:00
abstract：:Pseudovascular adenoid squamous cell carcinoma (PASCC) is a variant epithelial neoplasm with the ability to simulate the growth pattern of angiosarcoma. It has been documented in the breast, skin, and, recently, lung. We describe three additional examples of pulmonary PASCC occurring in two men and one woman. The pati...
journal_title：Human pathology
pub_type： 杂志文章
doi：10.1016/0046-8177(94)90145-7
更新日期：1994-04-01 00:00:00
abstract：:The types of collagen present in a case of elastofibroma dorsi were determined using type specific, characterized collagen antibodies. The presence of type II collagen (normally present only in articular cartilage and in selected ocular structures) is discussed with regard to the pathogenesis of this lesion, and the u...
journal_title：Human pathology
pub_type： 杂志文章
doi：10.1016/s0046-8177(81)80107-4
更新日期：1981-02-01 00:00:00
abstract：:Mesenchymal chondrosarcoma, a rare malignant round cell and hyaline cartilage tumor, is most commonly intraosseous but can occur in extraskeletal sites. We intensively observed the morphology and applied Sox9 (master regulator of chondrogenesis), beta-catenin (involved in bone formation, thought to inhibit chondrogene...
journal_title：Human pathology
pub_type： 杂志文章
doi：10.1016/j.humpath.2009.11.006
更新日期：2010-05-01 00:00:00
abstract：:Kaposi's sarcoma (KS) is a pleomorphic spindle cell lesion whose incidence has markedly increased among patients infected with the human immunodeficiency virus (HIV), especially among those whose primary risk factor is homo/bisexual transmission. The question as to whether KS is even a true neoplasm still remains larg...
journal_title：Human pathology
pub_type： 杂志文章
doi：10.1016/0046-8177(92)90112-g
更新日期：1992-03-01 00:00:00
abstract：:Decreases in true expenses in the hospital clinical laboratory consequent to reduced utilization were estimated from four models. Each category of direct expense was adjusted according to projections and confirmed to the extent possible by actual experience. No projections of reductions in indirect expenses were made....
journal_title：Human pathology
pub_type： 杂志文章
doi：10.1016/s0046-8177(84)80002-7
更新日期：1984-06-01 00:00:00
abstract：:Diffuse gastric cancer, characterized by poorly cohesive, diffusely infiltrating cells with no or little gland formation, is known to show several morphologic variants, but their prognostic value, if any, is poorly documented. In this article, 119 cases of invasive (T1b to T4) diffuse gastric cancer, which had undergo...
journal_title：Human pathology
pub_type： 杂志文章
doi：10.1016/j.humpath.2009.04.004
更新日期：2009-11-01 00:00:00
abstract：:The introduction of next-generation sequencing has broadened the genetic landscape of myeloproliferative neoplasms (MPNs) beyond JAK2, MPL, and CALR. However, the biological role and clinical impact of most other mutations are not well defined. We interrogated 101 genes in 143 BCR-ABL1-negative MPNs in chronic phase f...
journal_title：Human pathology
pub_type： 杂志文章
doi：10.1016/j.humpath.2018.11.022
更新日期：2019-04-01 00:00:00
abstract：:Among 236 aortic valves surgically excised at the Mayo Clinic in 1990 (mean patient age, 66 years; age range, 10 to 92 years), 154 (65%) were stenotic, 58 (25%) were insufficient, and 24 (10%) were both stenotic and insufficient. Pure stenosis was related to calcification, and causes included degenerative (51%), bicus...
journal_title：Human pathology
pub_type： 杂志文章
doi：10.1016/0046-8177(93)90267-k
更新日期：1993-12-01 00:00:00
abstract：:Acinar cell carcinoma (ACC) is a rare tumor that differentiates toward pancreatic acinar cells and shows evidence of pancreatic enzyme production. Mixed acinar-neuroendocrine carcinoma (MANC) is defined as having more than 30% of both acinar and neuroendocrine cell types as per immunohistochemistry analysis. Trypsin i...
journal_title：Human pathology
pub_type： 杂志文章
doi：10.1016/j.humpath.2020.07.019
更新日期：2020-09-01 00:00:00
abstract：:Microsatellite instability (MSI) defines a specific type of genetic instability. Although consensus diagnostic criteria for MSI definition in colorectal cancer have been established, their utility in other tumor types remain to be proven. Previously we developed a mathematical model for MSI definition in colorectal ca...
journal_title：Human pathology
pub_type： 杂志文章
doi：10.1016/j.humpath.2003.10.021
更新日期：2004-03-01 00:00:00
abstract：:Phyllodes tumors are rare but clinically important fibroepithelial tumors of the breast. Both epithelial and stromal components actively interact with each other to participate in phyllodes tumor development. Accumulated evidence suggests that the Wnt signaling pathway is important in this stromal-epithelial interacti...
journal_title：Human pathology
pub_type： 杂志文章
doi：10.1016/j.humpath.2012.03.028
更新日期：2012-12-01 00:00:00
abstract：:The control of colorectal cancer is currently dependent on early detection, and its prevention requires the recognition and treatment of its precursor lesions. The adenoma has been established as the precursor of colorectal carcinoma in the general population. Among patients with inflammatory bowel disease (IBD), dysp...
journal_title：Human pathology
pub_type： 杂志文章,评审
doi：10.1016/0046-8177(94)90032-9
更新日期：1994-11-01 00:00:00
abstract：:The cerebrum, cerebellum, and choroid plexuses from 16 patients with systemic amyloidosis, and the pituitary glands from 14 of these patients, were investigated histologically and immunohistochemically. Cerebrovascular amyloid (CVA) was found in the leptomeninges and cortices of six patients with systemic amyloidosis,...
journal_title：Human pathology
pub_type： 杂志文章
doi：10.1016/0046-8177(89)90102-0
更新日期：1989-09-01 00:00:00
abstract：:Mutually exclusive histone 3.3 gene mutations have been recognized in chondroblastoma and giant cell tumor of bone (GCTB), which may be useful for differential diagnostic purposes in morphologically ambiguous cases. Although more than 90% of GCTBs present histone 3.3 variants exclusively in the H3F3A gene, chondroblas...
journal_title：Human pathology
pub_type： 杂志文章
doi：10.1016/j.humpath.2017.08.033
更新日期：2017-10-01 00:00:00
abstract：:To increase awareness of the possible coexistence of an ovarian and breast carcinoma in a BRCA1 mutation carrier, we describe a patient with primary ovarian cancer who developed distant metastases after 3 years and again after 6 years. Surprisingly, at that time an occult breast carcinoma was identified to be the orig...
journal_title：Human pathology
pub_type： 杂志文章
doi：10.1016/j.humpath.2003.11.008
更新日期：2004-05-01 00:00:00
abstract：:Histone methyltransferase (HMT), which catalyzes a histone methylation, is frequently altered in cancers at mutation and expression levels. The aims of this study were to explore whether SETD1B, SETDB2, and SETD2, SET domain-containing HMT genes, are mutated and expressionally altered in gastric (GC) and colorectal ca...
journal_title：Human pathology
pub_type： 杂志文章
doi：10.1016/j.humpath.2014.04.013
更新日期：2014-08-01 00:00:00
abstract：:Alterations of the epidermal growth factor receptor (EGFR) can be observed in a significant subset of esophageal adenocarcinomas (EACs), and targeted therapy against EGFR may become an interesting approach for the treatment of these tumors. Mutations of KRAS, NRAS, BRAF, and phosphatidylinositol-3-kinase catalytic sub...
journal_title：Human pathology
pub_type： 杂志文章
doi：10.1016/j.humpath.2012.08.005
更新日期：2013-05-01 00:00:00
abstract：:Giant cell tumor of tendon sheath (GCTS) and pigmented villonodular synovitis (PVNS) are common synovial "tumors." Their immunohistochemical profile, however, has not been well characterized, and uncertainty exists regarding their histogenesis and relationship to fibroma of tendon sheath. In an effort to clarify these...
journal_title：Human pathology
pub_type： 杂志文章
doi：10.1016/0046-8177(95)90226-0
更新日期：1995-07-01 00:00:00
abstract：:Multifocal/multicentric breast cancers are common. However, investigations of biomarkers such as estrogen receptor, progesterone receptor, and human epidermal growth factor receptor 2 in individual tumor foci of such cancers are rare. This study was designed to evaluate the status of the hormone receptors, human epide...
journal_title：Human pathology
pub_type： 杂志文章
doi：10.1016/j.humpath.2010.08.026
更新日期：2012-01-01 00:00:00
abstract：:Esophageal cancer is one of the most fatal cancers worldwide and is characterized by great variation in rates among different populations. Linxian, a county in Henan Province, located in north-central China, has one of the highest rates of esophageal squamous cell carcinoma in the world. Most squamous cell carcinomas ...
journal_title：Human pathology
pub_type： 杂志文章
doi：10.1016/s0046-8177(98)90260-x
更新日期：1998-11-01 00:00:00
abstract：:In 1821 Napoleon died in exile on the Island of St. Helena. Although the autopsy had suggested stomach cancer as the cause of death, in 1961 an elevated arsenic concentration was found in Napoleon's hair. This finding elicited numerous theories of conspiracy, treachery, and poisoning. Most recent reports even suggeste...
journal_title：Human pathology
pub_type： 传,历史文章,杂志文章
doi：10.1016/j.humpath.2005.02.001
更新日期：2005-04-01 00:00:00
abstract：:The vascular endothelial growth factor (VEGF) family and its receptors have multifunctional activities besides angiogenesis, and some of these molecules are induced by hypoxia/ischemia. They are known to be expressed in human placenta, but little is known about their involvement in pathologic conditions. We have inves...
journal_title：Human pathology
pub_type： 杂志文章
doi：10.1053/hupa.2002.129420
更新日期：2002-11-01 00:00:00
abstract：:Gene expression studies have identified a basal phenotype of breast cancer; these are hormone receptor and HER2-negative cancers with poor prognosis. High levels of cyclin E and Skp2, and low levels of p27 have previously been individually associated with both basal-like breast cancer and a poor outcome after diagnosi...
journal_title：Human pathology
pub_type： 杂志文章
doi：10.1016/j.humpath.2008.03.004
更新日期：2008-10-01 00:00:00
abstract：:Four different genetic abnormalities may occur in endometrioid adenocarcinomas of the endometrium (mircosatellite instability and mutations in the PTEN, k-RAS and beta-catenin genes), whereas nonendometrioid carcinomas of the endometrium often have p53 mutations and loss of heterozygosity on several chromosomes. Occas...
journal_title：Human pathology
pub_type： 杂志文章,评审
doi：10.1053/hupa.2001.25929
更新日期：2001-06-01 00:00:00
abstract：:Pleomorphic adenomas of the breast (PAB) are uncommon tumors. We studied the clinicopathologic features of 10 cases of PAB, seven of which were assessed immunohistochemically. Nine patients were women, with a median age at diagnosis of 65 years. Eight patients presented with a palpable mass; two had a nipple discharge...
journal_title：Human pathology
pub_type： 杂志文章,评审
doi：10.1016/0046-8177(91)90102-u
更新日期：1991-12-01 00:00:00
abstract：:In two patients with malignant germ cell tumors angiosarcoma developed through two apparently different mechanisms. In one case the angiosarcoma probably developed as a complication of therapeutic radiation, since radiation changes were demonstrated in tissue adjacent to the neoplasm and since the angiosarcoma was not...
journal_title：Human pathology
pub_type： 杂志文章
doi：10.1016/s0046-8177(85)80013-7
更新日期：1985-03-01 00:00:00
abstract：:The family of the poly(adenosine diphosphate-ribose) polymerase (PARP) proteins is directly involved in genomic stability, DNA repair, and apoptosis by DNA damage. In this study, we evaluated the role of PARP-1 in melanoma and its prognostic importance. We studied by immunohistochemistry and Western blot analysis PARP...
journal_title：Human pathology
pub_type： 杂志文章
doi：10.1016/j.humpath.2005.04.017
更新日期：2005-07-01 00:00:00
abstract：:Ewing sarcoma (ES) is an aggressive malignant small round cell tumor that arises in bone or soft tissue of adolescents and young adults. A characteristic molecular finding in ES is EWSR1 gene fusion with ETS (erythroblast transformation-specific) family genes including FLI1 (~90%) and ERG (>5%). Here we report our exp...
journal_title：Human pathology
pub_type： 杂志文章
doi：10.1016/j.humpath.2013.03.003
更新日期：2013-10-01 00:00:00
