Relaxation effects of ferucarbotran-labeled mesenchymal stem cells at 1.5T and 3T: discrimination of viable from lysed cells.

Abstract:

:Human mesenchymal stem cells (hMSCs) were labeled with Ferucarbotran by simple incubation and cultured for up to 14 d. Iron content was determined by spectrometry and the intracellular localization of the contrast agent uptake was studied by electron and confocal microscopy. At various time points after labeling, ranging from 1 to 14 d, samples with viable or lysed labeled hMSCs, as well as nonlabeled controls, underwent MRI. Spin-echo (SE) and gradient-echo (GE) sequences with multiple TRs and TEs were used at 1.5T and 3T on a clinical scanner. Spectrometry showed an initial iron oxide uptake of 7.08 pg per cell. Microscopy studies revealed lysosomal compartmentalization. Contrast agent effects of hMSCs were persistent for up to 14 d after labeling. A marked difference in the T(2) effect of compartmentalized iron oxides compared to free iron oxides was found on T(2)-weighted sequences, but not on T(2)*-weighted sequences. The observed differences may be explained by the loss of compartmentalization of iron oxide particles, the uniformity of distribution, and the subsequent increase in dephasing of protons on SE images. These results show that viable cells with compartmentalized iron oxides may-in principle-be distinguished from lysed cells or released iron oxides.

journal_name

Magn Reson Med

authors

Henning TD,Wendland MF,Golovko D,Sutton EJ,Sennino B,Malek F,Bauer JS,McDonald DM,Daldrup-Link H

doi

10.1002/mrm.22011

subject

Has Abstract

pub_date

2009-08-01 00:00:00

pages

325-32

issue

2

eissn

0740-3194

issn

1522-2594

journal_volume

62

pub_type

杂志文章
  • Piezoelectric-controlled tuning capacitor for surface coils.

    abstract::The design of a piezoelectric-controlled trimmer capacitor and its use as a tuning element of an MRI surface coil at 63.8 MHz are described. It is found that image quality does not depend strongly on match of the coil to the transmission line but that tuning can be a significant factor. The work is intended to lead ev...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.1910120106

    authors: Hyde JS,Rilling RJ,Jesmanowicz A,Kneeland JB

    更新日期:1989-10-01 00:00:00

  • Low-power NMR volume selection by slicing z magnetization.

    abstract::A novel method for reducing the rf power requirements of selective pulses employed for z magnetization slice inversion in localized NMR spectroscopy is presented. Following slice preparation by the use of frequency-incremented sinc pulses, the slice gradient is reduced, which narrows the frequency width of the slice. ...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.1910050610

    authors: Brereton IM,Brooks WM,Marshman MF,Field J,Doddrell DM

    更新日期:1987-12-01 00:00:00

  • A continuum of T 2 * components: Flexible fast fraction mapping in sodium MRI.

    abstract:PURPOSE:Parameter mapping in sodium MRI data is challenging due to inherently low SNR and spatial resolution, prompting the need to employ robust models and estimation techniques. This work aims to develop a continuum model of sodium T 2 ...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.27659

    authors: Syeda W,Blunck Y,Kolbe S,Cleary JO,Johnston LA

    更新日期:2019-06-01 00:00:00

  • Dynamic 13C NMR analysis of pyruvate and lactate oxidation in the in vivo canine myocardium: evidence of reduced utilization with increased work.

    abstract::In this work, substrate selection was monitored in the left ventricle of the canine myocardium by following pyruvate and lactate oxidation under in vivo conditions at basal and elevated workloads. These studies were conducted in the open chest model using dynamic 13C NMR techniques in the presence and absence of dichl...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.1910380608

    authors: Rath DP,Zhu H,Tong X,Jiang Z,Hamlin RL,Robitaille PM

    更新日期:1997-12-01 00:00:00

  • Potential degradation in image quality due to selective averaging of phase-encoding lines in Fourier transform MRI.

    abstract::The properties of the image noise for selective averaging of phase-encoding lines have been investigated. For a given acquisition time, selective averaging results in a signal-to-noise ratio penalty compared to averaging all lines equally. In addition, selective averaging yields a non uniform distribution of noise cha...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.1910190115

    authors: Mugler JP 3rd

    更新日期:1991-05-01 00:00:00

  • Differentiation of human tumors from nonmalignant tissue by natural-abundance 13C NMR spectroscopy.

    abstract::High-quality, high-resolution, proton-decoupled natural-abundance 13C NMR spectra have been obtained in vitro at 100.6 MHz from unprocessed human pathology specimens of tumors and adjacent nonneoplastic control tissues from lung, colon, and prostate. In these preliminary studies, specific molecular parameters were ide...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.1910070403

    authors: Halliday KR,Fenoglio-Preiser C,Sillerud LO

    更新日期:1988-08-01 00:00:00

  • The sensitivity of low flip angle RARE imaging.

    abstract::It is demonstrated that the stability of the Carr-Purcell-Meiboom-Gill (CPMG) sequence reflects the existence of a steady state solution to the Bloch equations in the absence of T2 and T1 decay. The steady state theory is then used to evaluate the performance of low flip angle RARE imaging sequences with both constant...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.1910370206

    authors: Alsop DC

    更新日期:1997-02-01 00:00:00

  • Altered white matter diffusion anisotropy in normal and preterm infants at term-equivalent age.

    abstract::To investigate white matter (WM) development, voxelwise analyses of diffusion tensor MRI (DTMRI) data, acquired from 12 very preterm and 11 preterm infants with gestational ages (GA) ranging from 25 to 29 and 29 to 32 weeks, respectively, and 10 newborn normal term infants were performed. T(2) relaxation measures were...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.21689

    authors: Rose SE,Hatzigeorgiou X,Strudwick MW,Durbridge G,Davies PS,Colditz PB

    更新日期:2008-10-01 00:00:00

  • Dynamic 31 P spectroscopic imaging of skeletal muscles combining flyback echo-planar spectroscopic imaging and compressed sensing.

    abstract:PURPOSE:Dynamic phosphorus MR spectroscopic imaging (31 P-MRSI) experiments require temporal resolution on the order of seconds to concurrently assess different muscle groups. A highly accelerated pulse sequence combining flyback echo-planar spectroscopic imaging (EPSI) and compressed sensing was developed and tested i...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.27682

    authors: Santos-Díaz A,Harasym D,Noseworthy MD

    更新日期:2019-06-01 00:00:00

  • High-resolution slice-selective Fourier velocity encoding in congenital heart disease using spiral SENSE with velocity unwrap.

    abstract::Quantification of peak velocity is important in the assessment of stenotic flow jets in patients with congenital heart disease. Phase-contrast magnetic resonance underestimates peak velocities. Hence, clinically Doppler ultrasound is used as the reference standard for assessing stenoses. It is possible to accurately m...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.24293

    authors: Steeden JA,Jones A,Pandya B,Atkinson D,Taylor AM,Muthurangu V

    更新日期:2012-06-01 00:00:00

  • Atraumatic quantitation of cerebral perfusion in cats by 19F magnetic resonance imaging.

    abstract::We have noninvasively produced low-resolution, quantitative nuclear magnetic resonance images of cerebral blood flow in 2-ml voxels in eight cats. Typical signal-to-noise of 4 to 1 was obtained in cerebral voxels in 16.5-s epochs. Mean flow during normocapnia (paCO2 = 39 +/- 4 mm Hg) and hypercapnia (paCO2 = 62 +/- 4 ...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.1910280105

    authors: Branch CA,Ewing JR,Helpern JA,Ordidge RJ,Butt S,Welch KM

    更新日期:1992-11-01 00:00:00

  • Phase constrained encoding (PACE): a technique for MRI in large static field inhomogeneities.

    abstract::In spin echo imaging, magnetization is assigned to a location defined by its frequency of rotation. In the presence of a static magnetic field inhomogeneity, however, this location does not correspond to the true location of the magnetization. This paper describes a magnetic resonance imaging technique called phase co...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.1910330407

    authors: Kim JK,Plewes DB,Henkelman RM

    更新日期:1995-04-01 00:00:00

  • Observing curved flow using RUFIS.

    abstract::The rotating ultra-fast imaging sequence (RUFIS) is used to image spins flowing through the curved portion of a 180 degrees U-tube with a circular cross section and a ratio of inner diameter to curvature diameter of 0.41. A velocity-encoding preparation sequence is used with RUFIS to measure quantitatively the axial v...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/(sici)1522-2594(199906)41:6<1258::aid-mrm2

    authors: Gach HM,Lowe IJ

    更新日期:1999-06-01 00:00:00

  • Real-time MR elastography for viscoelasticity quantification in skeletal muscle during dynamic exercises.

    abstract:PURPOSE:To develop and test real-time MR elastography for viscoelastic parameter quantification in skeletal muscle during dynamic exercises. METHODS:In 15 healthy participants, 6 groups of lower-leg muscles (tibialis anterior, tibialis posterior, peroneus, extensor digitorum longus, soleus, gastrocnemius) were investi...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.28095

    authors: Schrank F,Warmuth C,Görner S,Meyer T,Tzschätzsch H,Guo J,Uca YO,Elgeti T,Braun J,Sack I

    更新日期:2020-07-01 00:00:00

  • Dextran-magnetite: a contrast agent for sodium-23 MRI.

    abstract::Dextran-magnetite, a superparamagnetic compound, is a powerful relaxation reagent for sodium. Administered intravenously, it is confined mainly to the vasculature and eliminates the signal from plasma sodium, a significant component of the tissue sodium signal. Applications of dextran-magnetite for in vivo sodium imag...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.1910080406

    authors: Summers RM,Joseph PM,Renshaw PF,Kundel HL

    更新日期:1988-12-01 00:00:00

  • A 1H-NMR method for determining temperature in cell culture perfusion systems.

    abstract::This report describes a noninvasive 1H-NMR method for measuring absolute temperatures (+/- 0.2 degrees C) in biological samples and, in particular, in cell culture perfusion systems, utilizing the linear temperature dependence of the water chemical shift relative to the temperature-independent shift of one of the comp...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.1910290120

    authors: Lutz NW,Kuesel AC,Hull WE

    更新日期:1993-01-01 00:00:00

  • Extended graphical model for analysis of dynamic contrast-enhanced MRI.

    abstract::Kinetic analysis with mathematical models has become increasingly important to quantify physiological parameters in computed tomography (CT), positron emission tomography (PET), and dynamic contrast-enhanced MRI (DCE-MRI). The modified Kety/Tofts model and the graphical (Patlak) model have been widely applied to DCE-M...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.22819

    authors: Chen H,Li F,Zhao X,Yuan C,Rutt B,Kerwin WS

    更新日期:2011-09-01 00:00:00

  • Multifrequency reconstruction for frequency-modulated bSSFP.

    abstract:PURPOSE:Banding artifacts in images acquired by balanced steady-state free precession (bSSFP) remain a challenge in MRI as they considerably reduce image quality, and diagnostic value deteriorates accordingly. As the steady-state tolerates small shifts in frequency, it is possible to acquire frequency-modulated bSSFP. ...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.26630

    authors: Slawig A,Wech T,Ratz V,Tran-Gia J,Neubauer H,Bley T,Köstler H

    更新日期:2017-12-01 00:00:00

  • Signal-to-noise ratio and spectral linewidth improvements between 1.5 and 7 Tesla in proton echo-planar spectroscopic imaging.

    abstract::This study characterizes gains in sensitivity and spectral resolution of proton echo-planar spectroscopic imaging (PEPSI) with increasing magnetic field strength (B(0)). Signal-to-noise ratio (SNR) per unit volume and unit time, and intrinsic linewidth (LW) of N-acetyl-aspartate (NAA), creatine (Cr), and choline (Cho)...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.21067

    authors: Otazo R,Mueller B,Ugurbil K,Wald L,Posse S

    更新日期:2006-12-01 00:00:00

  • fMRI of auditory stimulation with intermolecular double-quantum coherences (iDQCs) at 1.5T.

    abstract::An intermolecular double-quantum coherence (iDQC) imaging technique was used to study auditory activation in the human brain at 1.5T with a dual temporal lobe surface phased array coil and a quadrature head coil. Preliminary results demonstrate that it is feasible to obtain auditory activation maps using iDQC imaging ...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/1522-2594(200103)45:3<356::aid-mrm1046>3.0

    authors: Zhong J,Kwok E,Chen Z

    更新日期:2001-03-01 00:00:00

  • Chemically induced analgesic nephropathy in the rat monitored by proton-electron double-resonance imaging (PEDRI).

    abstract::Proton-electron double-resonance imaging (PEDRI) was used to assess renal function by monitoring the flow of the exogenous nitroxide free radical proxyl carboxylic acid (PCA) through normal and injured kidneys in the living rat. Kidney damage was induced by treatment with 2-bromoethylamine (BEA), which provides a well...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.1910400214

    authors: Seimenis I,Foster MA,Lurie DJ,Hutchison JM,Whiting PH,Payne S

    更新日期:1998-08-01 00:00:00

  • Accelerated three-dimensional multispectral MRI with robust principal component analysis for separation of on- and off-resonance signals.

    abstract:PURPOSE:To enable highly accelerated distortion-free MRI near metal by separating on- and off-resonance to exploit the redundancy of slice-phase encoding for the dominant on-resonance component. METHODS:Multispectral MRI techniques resolve off-resonance distortions by a combination of limited excitation bins and addit...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.26819

    authors: Levine E,Stevens K,Beaulieu C,Hargreaves B

    更新日期:2018-03-01 00:00:00

  • Evidence for enhanced functional activity of cervical cord in relapsing multiple sclerosis.

    abstract::Functional MRI (fMRI) was used to assess proprioceptive-associated cervical cord activity in 24 relapsing multiple sclerosis (MS) patients and 10 controls. Cord and brain conventional and diffusion tensor (DT) MRI were also acquired. fMRI was performed using a block design during a proprioceptive stimulation consistin...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.21595

    authors: Agosta F,Valsasina P,Rocca MA,Caputo D,Sala S,Judica E,Stroman PW,Filippi M

    更新日期:2008-05-01 00:00:00

  • Motion corrected water/fat whole-heart coronary MR angiography with 100% respiratory efficiency.

    abstract:PURPOSE:To develop a framework for respiratory motion-corrected 3D whole-heart water/fat coronary MR angiography (CMRA) at 3T with reduced and predictable scan time. METHODS:A 3D dual-echo acquisition and respiratory motion-corrected reconstruction framework for water/fat CMRA imaging was developed. The acquisition se...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.27732

    authors: Munoz C,Cruz G,Neji R,Botnar RM,Prieto C

    更新日期:2019-08-01 00:00:00

  • Pulmonary perfusion: qualitative assessment with dynamic contrast-enhanced MRI using ultra-short TE and inversion recovery turbo FLASH.

    abstract::The accurate assessment of pulmonary perfusion is especially important in the evaluation of patients with suspected pulmonary embolism, a common and potentially lethal disorder that can be treated by aggressive anticoagulation. In this study, we demonstrate for the first time the use of MR to image pulmonary perfusion...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.1910360402

    authors: Hatabu H,Gaa J,Kim D,Li W,Prasad PV,Edelman RR

    更新日期:1996-10-01 00:00:00

  • Detection and volume determination of colonic tumors in Min mice by magnetic resonance micro-imaging.

    abstract::We applied MRI to the in vivo detection of spontaneous colorectal tumors in a unique mouse model, the Fox Chase Cancer Center (FCCC) ApcMIN mouse. Unlike other Min (multiple intestinal neoplasia) strains, FCCC ApcMIN animals develop an appreciable number of tumors in the large intestine, which makes them an appropriat...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.20175

    authors: Hensley HH,Chang WC,Clapper ML

    更新日期:2004-09-01 00:00:00

  • Whole-heart chemical shift encoded water-fat MRI.

    abstract:PURPOSE:To develop and evaluate a free-breathing chemical-shift-encoded (CSE) spoiled gradient-recalled echo (SPGR) technique for whole-heart water-fat imaging at 3 Tesla (T). METHODS:We developed a three-dimensional (3D) multi-echo SPGR pulse sequence with electrocardiographic gating and navigator echoes and evaluate...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.24982

    authors: Taviani V,Hernando D,Francois CJ,Shimakawa A,Vigen KK,Nagle SK,Schiebler ML,Grist TM,Reeder SB

    更新日期:2014-09-01 00:00:00

  • Commissioning of MRI-only based treatment planning procedure for external beam radiotherapy of prostate.

    abstract::In radiotherapy, target tissues are defined best on MR images due to their superior soft tissue contrast. Computed tomography imaging is geometrically accurate and it is needed for dose calculation and generation of reference images for treatment localization. Co-registration errors between MR and computed tomography ...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章,随机对照试验

    doi:10.1002/mrm.24459

    authors: Kapanen M,Collan J,Beule A,Seppälä T,Saarilahti K,Tenhunen M

    更新日期:2013-07-01 00:00:00

  • Rapid T1 mapping of mouse myocardium with saturation recovery Look-Locker method.

    abstract::Dynamic contrast-enhanced MRI using gadolinium or manganese provides unique characterization of myocardium and its pathology. In this study, an electrocardiography (ECG) triggered saturation recovery Look-Locker method was developed and validated for fast cardiac T(1) mapping in small animal models. By sampling the in...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.22544

    authors: Li W,Griswold M,Yu X

    更新日期:2010-11-01 00:00:00

  • Sources of functional apparent diffusion coefficient changes investigated by diffusion-weighted spin-echo fMRI.

    abstract::The mechanism behind previously observed changes in the apparent diffusion coefficient (ADC) during brain activation is not well understood. Therefore, we investigated the signal source and spatial specificity of functional magnetic resonance imaging (fMRI) ADC changes systematically in the visual cortex of cats using...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.21074

    authors: Jin T,Zhao F,Kim SG

    更新日期:2006-12-01 00:00:00