A 1H-NMR method for determining temperature in cell culture perfusion systems.

Abstract:

:This report describes a noninvasive 1H-NMR method for measuring absolute temperatures (+/- 0.2 degrees C) in biological samples and, in particular, in cell culture perfusion systems, utilizing the linear temperature dependence of the water chemical shift relative to the temperature-independent shift of one of the components of the biological medium, e.g., pyruvate, acetate or lactate. The effects of flow on temperature can be monitored and appropriate adjustment of the temperature controller can be made.

journal_name

Magn Reson Med

authors

Lutz NW,Kuesel AC,Hull WE

doi

10.1002/mrm.1910290120

subject

Has Abstract

pub_date

1993-01-01 00:00:00

pages

113-8

issue

1

eissn

0740-3194

issn

1522-2594

journal_volume

29

pub_type

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