Abstract：
:We have identified a novel octamer binding factor (Oct-3) in P19 embryonal carcinoma cells. Oct-3, which recognizes the typical octamer motif (ATTTGCAT) as well as the AT-rich sequence TTAAAATTCA, is present in P19 stem cells but disappears when the cells are induced to differentiate by retinoic acid (RA). Cloned cDNA corresponding to Oct-3 encodes a protein of 377 amino acids. Oct-3 has a conserved POU domain, but the remaining part is distinct from other POU domain-containing proteins such as Oct-1 and Oct-2. mRNA of 1.5 kb coding for Oct-3 is abundant in P19 stem cells but is dramatically repressed during RA-induced differentiation. Repression of the 1.5 kb mRNA is rapid and specific to RA. In mouse, oct-3 mRNA is undetectable in all the adult organs examined. The N-terminal proline-rich region of Oct-3, when fused to the DNA binding domain of c-Jun, functions as a transcriptional activating domain. We suggest that Oct-3 is a novel octamer binding transcription factor that is developmentally regulated during mouse embryogenesis.
journal_name
Celljournal_title
Cellauthors
Okamoto K,Okazawa H,Okuda A,Sakai M,Muramatsu M,Hamada Hdoi
10.1016/0092-8674(90)90597-8subject
Has Abstractpub_date
1990-02-09 00:00:00pages
461-72issue
3eissn
0092-8674issn
1097-4172pii
0092-8674(90)90597-8journal_volume
60pub_type
杂志文章相关文献
CELL文献大全abstract：:We have reconstituted the fusion and assembly of vesiculated Golgi membranes (VGMs) into functionally active stacks of cisternae. A kinetic analysis of this assembly process revealed that highly dispersed VGMs of 60-90 nm diameter first fuse to form larger vesicles of 200-300 nm diameter that are clustered together. T...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(95)90269-4
更新日期：1995-09-22 00:00:00
abstract：:Mutations at the X-linked disconnected locus of D. melanogaster lead to the failure of adult photoreceptor axons to innervate their target cells in the developing optic lobes of the third instar larva, resulting in flies that have rudimentary optic ganglia. The cascade of epigenetic events leading to the adult disconn...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(87)90180-2
更新日期：1987-09-25 00:00:00
abstract：:As axons grow past intermediate targets, they change their responsiveness to guidance cues. Local upregulation of receptor expression is involved, but the mechanisms for this are not clear. Here protein synthesis is traced within individual axons by introducing RNAs encoding visualizable reporters. Individual severed ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(02)00813-9
更新日期：2002-07-26 00:00:00
abstract：:The auditory inner hair cell (IHC) ribbon synapse operates with an exceptional temporal precision and maintains a high level of neurotransmitter release. However, the molecular mechanisms underlying IHC synaptic exocytosis are largely unknown. We studied otoferlin, a predicted C2-domain transmembrane protein, which is...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2006.08.040
更新日期：2006-10-20 00:00:00
abstract：:Epigenetics has recently evolved from a collection of diverse phenomena to a defined and far-reaching field of study. In this Essay, we examine the epistemology of epigenetics, provide a brief overview of underlying molecular mechanisms, and suggest future challenges for the field. ...
journal_title：Cell
pub_type： 杂志文章,评审
doi：10.1016/j.cell.2007.02.006
更新日期：2007-02-23 00:00:00
abstract：:The commitment complex is the first defined step in the yeast (S. cerevisiae) splicing pathway. It contains U1 snRNP as well as Mud2p, which resembles human U2AF65. In a genetic screen, we identified the yeast gene MSL-5, which is a novel commitment complex component. Genetic and biochemical criteria indicate a direct...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(00)80221-4
更新日期：1997-05-02 00:00:00
abstract：:The mechanisms by which immune checkpoint blockade modulates tumor evolution during therapy are unclear. We assessed genomic changes in tumors from 68 patients with advanced melanoma, who progressed on ipilimumab or were ipilimumab-naive, before and after nivolumab initiation (CA209-038 study). Tumors were analyzed by...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2017.09.028
更新日期：2017-11-02 00:00:00
abstract：:The susceptibility to repair of particular base mismatches by the hex system of Streptococcus pneumoniae was examined by comparison of the nucleotide sequence of the wild-type and eight mutant alleles of the malM gene. A detailed restriction map was constructed for pLS70, and the nucleotide sequence was determined for...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(82)90126-x
更新日期：1982-12-01 00:00:00
abstract：:We have identified a gene, SRV2, mutations of which alleviate stress sensitivity in strains carrying an activated RAS gene. Epistasis analysis suggests that the gene affects accumulation of cAMP in the cell. Direct assays of cAMP accumulation indicate that mutations of the gene diminish the rate of in vivo production ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(90)90813-t
更新日期：1990-04-20 00:00:00
abstract：:Adult skeletal muscle generates force in a controlled and directed manner through the contraction of highly specialized, postmitotic, multinucleated myofibers. Life-long muscle function relies on maintenance and regeneration of myofibers through a highly regulated process beginning with activation of normally quiescen...
journal_title：Cell
pub_type： 杂志文章,评审
doi：10.1016/j.cell.2005.08.021
更新日期：2005-09-09 00:00:00
abstract：:A single mouse blastomere from an embryo until the 8-cell stage can generate an entire blastocyst. Whether laboratory-cultured cells retain a similar generative capacity remains unknown. Starting from a single stem cell type, extended pluripotent stem (EPS) cells, we established a 3D differentiation system that enable...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2019.09.029
更新日期：2019-10-17 00:00:00
abstract：:In female mammals a "random choice" mechanism decides which of the two X chromosomes will be inactivated. It has been postulated that Xist is crucial for heterochromatinization and thus functions downstream of the choice mechanism. Here we report that females heterozygous for an internal deletion in the Xist gene, whi...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(00)81133-2
更新日期：1998-03-06 00:00:00
abstract：:By chromosome walking in the major histocompatibility complex (MHC) of the BALB/c mouse, we have linked the K region to the I region at the molecular level. Forty-nine overlapping cosmid clones define a stretch of about 600 kb of DNA containing 2 class I and 7 class II genes. Eleven independent recombination events we...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(86)90012-7
更新日期：1986-03-28 00:00:00
abstract：:Amid growing recognition that a successful scientific career requires skills beyond scientific acumen, institutions are racing to provide management training for newly minted principal investigators. ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2008.03.008
更新日期：2008-03-21 00:00:00
abstract：:We have shown previously that the putative oncogene pim-1 is frequently activated by provirus insertion in murine leukemia virus-induced T cell lymphomas. Here we describe the structure of the pim-1 gene as determined by sequencing genomic and cDNA clones. The gene has an open reading frame, encoding a protein of 313 ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(86)90886-x
更新日期：1986-08-15 00:00:00
abstract：:The CD4 lymphocyte surface glycoprotein and the lck tyrosine protein kinase p56lck are found as a complex in T lymphocytes. We have defined the domains in both proteins that are responsible for this interaction by coexpressing hybrid and deleted forms of the two proteins in HeLa cells. We have found that the unique 32...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(89)90008-1
更新日期：1989-11-17 00:00:00
abstract：:Plants rely heavily on environmental cues to control the timing of developmental transitions. We are beginning to better understand what determines the timing of two of these transitions, the switch from juvenile to adult vegetative development and the transition to flowering. In this review, we discuss how RNA silenc...
journal_title：Cell
pub_type： 杂志文章,评审
doi：10.1016/j.cell.2006.05.005
更新日期：2006-05-19 00:00:00
abstract：:Ocean microbial communities strongly influence the biogeochemistry, food webs, and climate of our planet. Despite recent advances in understanding their taxonomic and genomic compositions, little is known about how their transcriptomes vary globally. Here, we present a dataset of 187 metatranscriptomes and 370 metagen...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2019.10.014
更新日期：2019-11-14 00:00:00
abstract：:The tyrT locus of E. coli contains a 208 bp spacer region that separates two copies of sequence encoding tRNATyr1. The spacer includes a 120 bp sequence that is homologous to a sequence that is repeated three times in the distal portion of the tyrT locus. The tyrT locus possesses a graded set of transcription terminat...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(81)90199-9
更新日期：1981-11-01 00:00:00
abstract：:It has been puzzling that mammalian telomeric proteins, including TRF1, TRF2, tankyrase, and TIN2 have no recognized orthologs in budding yeast. Here, we describe a human protein, hRap1, that is an ortholog of the yeast telomeric protein, scRap1p. hRap1 has three conserved sequence motifs in common with scRap1, is loc...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(00)80858-2
更新日期：2000-05-26 00:00:00
abstract：:We present genetic evidence that integrins regulate epithelial-mesenchymal interactions during organogenesis. Mice with a mutation in the alpha8 gene do not express the integrin alpha8 beta1 and exhibit profound deficits in kidney morphogenesis. In wild-type animals, inductive interactions between the ureteric epithel...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(00)81903-0
更新日期：1997-03-07 00:00:00
abstract：:Toxins have evolved to target regions of membrane ion channels that underlie ligand binding, gating, or ion permeation, and have thus served as invaluable tools for probing channel structure and function. Here, we describe a peptide toxin from the Earth Tiger tarantula that selectively and irreversibly activates the c...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2010.03.052
更新日期：2010-05-28 00:00:00
abstract：:The inhibitor of apoptosis (IAP) proteins suppress cell death by inhibiting the catalytic activity of caspases. Here we present the crystal structure of caspase-7 in complex with a potent inhibitory fragment from XIAP at 2.45 A resolution. An 18-residue XIAP peptide binds the catalytic groove of caspase-7, making exte...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(01)00272-0
更新日期：2001-03-09 00:00:00
abstract：:Hybrid plasmid molecules containing tandemly repeated Drosophila satellite DNA were constructed using a modification of the (dA)-(dT) homopolymer procedure of Lobban and Kaiser (1973). Recombinant plasmids recovered after transformation of recA bacteria contained 10% of the amount of satellite DNA present in the trans...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(77)90038-1
更新日期：1977-03-01 00:00:00
abstract：:Enteric-associated neurons (EANs) are closely associated with immune cells and continuously monitor and modulate homeostatic intestinal functions, including motility and nutrient sensing. Bidirectional interactions between neuronal and immune cells are altered during disease processes such as neurodegeneration or irri...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2019.12.002
更新日期：2020-01-09 00:00:00
abstract：:Loss of cell polarity proteins such as Scribble induces neoplasia in Drosophila by promoting uncontrolled proliferation. In mammals, the role that polarity proteins play during tumorigenesis is not well understood. Here, we demonstrate that depletion of Scribble in mammary epithelia disrupts cell polarity, blocks thre...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2008.09.045
更新日期：2008-11-28 00:00:00
abstract：:Negative feedback is a widespread feature of signaling pathways. In an unexpected twist described in this issue, He and colleagues identify a membrane-tethered metalloprotease named Tiki that inhibits Wnt signaling by removing an essential eight-residue fragment from Wnt itself. ...
journal_title：Cell
pub_type： 评论,杂志文章
doi：10.1016/j.cell.2012.06.007
更新日期：2012-06-22 00:00:00
abstract：:During development, the precise relationships between transcription and chromatin modifications often remain unclear. We use the X chromosome inactivation (XCI) paradigm to explore the implication of chromatin changes in gene silencing. Using female mouse embryonic stem cells, we initiate XCI by inducing Xist and then...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2018.11.041
更新日期：2019-01-10 00:00:00
abstract：:The Zika epidemic in the Americas has challenged surveillance and control. As the epidemic appears to be waning, it is unclear whether transmission is still ongoing, which is exacerbated by discrepancies in reporting. To uncover locations with lingering outbreaks, we investigated travel-associated Zika cases to identi...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2019.07.018
更新日期：2019-08-22 00:00:00
abstract：:The extracellular space (ECS) of the brain has an extremely complex spatial organization, which has defied conventional light microscopy. Consequently, despite a marked interest in the physiological roles of brain ECS, its structure and dynamics remain largely inaccessible for experimenters. We combined 3D-STED micros...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2018.02.007
更新日期：2018-02-22 00:00:00
