The essentiality of alpha-2-macroglobulin in human salivary innate immunity against new H1N1 swine origin influenza A virus.

abstract：:Induced sputum is a readily accessible biological fluid whose composition may alter as a consequence of disease. To date, however, the proteins that routinely populate this biofluid are largely unknown, in part due to the technical difficulties in processing such mucin-rich samples. To provide a catalogue of sputum pr...

journal_title：Proteomics

authors： Nicholas B,Skipp P,Mould R,Rennard S,Davies DE,O'Connor CD,Djukanović R

更新日期：2006-08-01 00:00:00

abstract：:Toxoplasma gondii infects a wide range of hosts worldwide, including humans and domesticated animals causing toxoplasmosis disease. Recently, exosomes, small extracellular vesicles (EV) that contain nucleic acids, proteins, and lipids derived from their original cells were linked with disease protection. The effect of...

journal_title：Proteomics

authors： Wowk PF,Zardo ML,Miot HT,Goldenberg S,Carvalho PC,Mörking PA

更新日期：2017-08-01 00:00:00

abstract：:We here present a user-friendly and extremely lightweight tool that can serve as a stand-alone front-end for the Open MS Search Algorithm (OMSSA) search engine, or that can directly be used as part of an informatics processing pipeline for MS driven proteomics. The OMSSA graphical user interface (OMSSAGUI) tool is wri...

journal_title：Proteomics

authors： Tharakan R,Martens L,Van Eyk JE,Graham DR

更新日期：2008-06-01 00:00:00

abstract：:Mammalian cells secrete two types of extracellular vesicles either constitutively or in a regulated manner: exosomes (50-100 nm in diameter) released from the intracellular compartment and ectosomes (also called microvesicles, 100-1000 nm in diameter) shed directly from the plasma membrane. Extracellular vesicles are ...

journal_title：Proteomics

authors： Choi DS,Kim DK,Kim YK,Gho YS

更新日期：2013-05-01 00:00:00

abstract：:The Human Kidney and Urine Proteome Project (HKUPP) was initiated in 2005 to promote proteomics research in the nephrology field, to better understand kidney functions as well as pathogenic mechanisms of kidney diseases, and to define novel biomarkers and therapeutic targets. This project was first approved in 2005 by...

journal_title：Proteomics

authors： Yamamoto T,Langham RG,Ronco P,Knepper MA,Thongboonkerd V

更新日期：2008-06-01 00:00:00

abstract：:Most cancers consist of heterogeneous populations of cells with substantial differences in tumorigenicity. Cells that possess self-renewal and tumor-initiating properties are often called cancer stem cells (CSCs). Since CSCs underlie tumor recurrence and metastasis and are resistant to current anti-cancer therapies, n...

journal_title：Proteomics

authors： Sawanobori A,Moriwaki K,Takamatsu S,Kamada Y,Miyoshi E

更新日期：2016-12-01 00:00:00

abstract：:Warm ischemia reperfusion (IR) injury of the liver is associated with changes in the expression and/or post-translational modification of numerous proteins. Only a few of these have been identified. We used 2-D DIGE to identify cytosolic proteins altered in the early stage of IR in an established rat model of segmenta...

journal_title：Proteomics

authors： Wilson CH,Zeile S,Chataway T,Nieuwenhuijs VB,Padbury RT,Barritt GJ

更新日期：2011-11-01 00:00:00

abstract：:Tumor development and progression consist of a series of complex processes involving multiple changes in gene expression (Paolo et al. Physiol. Rev., 1993, 73, 161-195; Lance et al. Cell., 1991, 64, 327-336). Tumor cells acquire an invasive and metastatic phenotype that is the main cause of death for cancer patients. ...

journal_title：Proteomics

authors： Hayashi E,Kuramitsu Y,Okada F,Fujimoto M,Zhang X,Kobayashi M,Iizuka N,Ueyama Y,Nakamura K

更新日期：2005-03-01 00:00:00

abstract：:Of all protein PTMs, glycosylation is by far the most common, and is a target for proteomic research. Glycosylation plays key roles in controlling various cellular processes and the modifications of the glycan structures in diseases highlight the clinical importance of this PTM. Glycosylation analysis remains a diffic...

journal_title：Proteomics

authors： Morelle W,Canis K,Chirat F,Faid V,Michalski JC

更新日期：2006-07-01 00:00:00

abstract：:Eukaryotic lipid rafts are membrane microdomains that have significant amounts of cholesterol and a selective set of proteins that have been associated with multiple biological functions. The Lyme disease agent, Borrelia burgdorferi, is one of an increasing number of bacterial pathogens that incorporates cholesterol o...

journal_title：Proteomics

authors： Toledo A,Pérez A,Coleman JL,Benach JL

更新日期：2015-11-01 00:00:00

abstract：:Staphylococcus aureus, a bacterial, food-borne pathogen of humans, can contaminate raw fruits and vegetables. While physical and chemical methods are available to control S. aureus, scientists are searching for inhibitory phytochemicals from plants. One promising compound from pomegranate is punicalagin, a natural ant...

journal_title：Proteomics

authors： Cooper B,Islam N,Xu Y,Beard HS,Garrett WM,Gu G,Nou X

更新日期：2018-05-01 00:00:00

abstract：:This paper reports on the findings of the Biomedical Research Institute, as one of the participants in the pilot study of the HUPO Brain Proteome Project. A biopsy and autopsy study sample derived from human brain was distributed among the participants for proteomic analysis. In our laboratory, attention was focused o...

journal_title：Proteomics

authors： Dumont D,Noben JP,Verhaert P,Stinissen P,Robben J

更新日期：2006-09-01 00:00:00

abstract：:Since the identification of approximately 25,000 proteins from the draft human genome assembly in 2001, estimates of the total have oscillated between 30,000 and 70,000. The recently announced genome closure has not generated a consensus gene count despite this being a key parameter for many areas of biology including...

journal_title：Proteomics

authors： Southan C

更新日期：2004-06-01 00:00:00

abstract：:Recently, we introduced a novel proteomics method employing a metalloendopeptidase with Lys-N specificity to produce proteolytic peptides. Fragmentation spectra generated by electron transfer dissociation, for a large proportion of the Lys-N proteolytic peptides, were found to be dominated by extensive series of c-typ...

journal_title：Proteomics

authors： van Breukelen B,Georgiou A,Drugan MM,Taouatas N,Mohammed S,Heck AJ

更新日期：2010-03-01 00:00:00

abstract：:Over the past decade, considerable progress has been made with respect to the analytical methods for analysis of glycans from biological sources. Regardless of the specific methods that are used, glycan analysis includes isolation, identification, and quantitation. Derivatization is indispensable to increase their ide...

journal_title：Proteomics

authors： Yang S,Rubin A,Eshghi ST,Zhang H

更新日期：2016-01-01 00:00:00

abstract：:Proteases are critical in many physiological processes and the human genome encodes for 566 predicted proteolytic enzymes. Therefore, there is great interest in identifying and characterizing physiologic protease-substrate relationships. The coagulation cascade is a well-described network of serine proteases. However,...

journal_title：Proteomics

authors： Niessen S,Hoover H,Gale AJ

更新日期：2011-06-01 00:00:00

abstract：:We describe the first phosphoproteome of the model filamentous fungus Aspergillus nidulans. Phosphopeptides were enriched using titanium dioxide, separated using a convenient ultra-long reverse phase gradient, and identified using a "high-high" strategy (high mass accuracy on the parent and fragment ions) with higher-...

journal_title：Proteomics

authors： Ramsubramaniam N,Harris SD,Marten MR

更新日期：2014-11-01 00:00:00

abstract：:In this study, we generated 40 somatic cell cloned (scNT) piglets. Of these, five piglets were stillborn, 22 scNT piglets died suddenly within the first week of life, and 1 piglet died after 40 days. Twelve scNT piglets are still healthy. The birth weights of compromised scNT piglets in comparison with those of normal...

journal_title：Proteomics

authors： Park MR,Cho SK,Lee SY,Choi YJ,Park JY,Kwon DN,Son WJ,Paik SS,Kim T,Han YM,Kim JH

更新日期：2005-05-01 00:00:00

abstract：:A proteome wide analysis was performed in Escherichia coli to identify the impact on protein N-termini of actinonin, an antibiotic specifically inhibiting peptide deformylase (PDF). A strategy and tool suite (SILProNaQ) was employed to provide large-scale quantitation of N-terminal modifications. In control conditions...

journal_title：Proteomics

authors： Bienvenut WV,Giglione C,Meinnel T

更新日期：2015-07-01 00:00:00

abstract：:Gene expression analyses of embryonic stem cells (ESCs) will help to uncover or further define signaling pathways and molecular mechanisms involved in the maintenance of self-renewal and pluripotency. We employed a 2-DE-based proteomics approach to analyze human ESC line, Royan H5, in undifferentiated cells and differ...

journal_title：Proteomics

authors： Fathi A,Pakzad M,Taei A,Brink TC,Pirhaji L,Ruiz G,Sharif Tabe Bordbar M,Gourabi H,Adjaye J,Baharvand H,Salekdeh GH

更新日期：2009-11-01 00:00:00

abstract：:Studying microbial interactions by MALDI mass spectrometry imaging (MSI) directly from growing media is a difficult task if high sensitivity is demanded. We present a quick and robust sample preparation strategy for growing fungi (Trichoderma atroviride, Rhizoctonia solani) on glass slides to establish a miniaturized ...

journal_title：Proteomics

authors： Holzlechner M,Reitschmidt S,Gruber S,Zeilinger S,Marchetti-Deschmann M

更新日期：2016-06-01 00:00:00

abstract：:High hydrostatic pressure (HHP) exerts diverse effects on microorganisms, leading to stress response and cell death. While inactivation of microorganisms by lethal HHP is well investigated in the context of food preservation and the hygienic safety of minimal food processes, sublethal HHP stress response and its effec...

journal_title：Proteomics

authors： Hörmann S,Scheyhing C,Behr J,Pavlovic M,Ehrmann M,Vogel RF

更新日期：2006-03-01 00:00:00

abstract：:Progress in MS-based methods for veterinary research and diagnostics is lagging behind compared to the human research, and proteome data of domestic animals is still not well represented in open source data repositories. This is particularly true for the equine species. Here we present a first Equine PeptideAtlas enco...

journal_title：Proteomics

authors： Bundgaard L,Jacobsen S,Sørensen MA,Sun Z,Deutsch EW,Moritz RL,Bendixen E

更新日期：2014-03-01 00:00:00

abstract：:Aphids are major insect pests of cereal crops, acting as virus vectors as well as causing direct damage. The responses of wheat to infestation by cereal aphid (Sitobion avenae) were investigated in a proteomic analysis. Approximately, 500 protein spots were reproducibly detected in the extracts from leaves of wheat se...

journal_title：Proteomics

authors： Ferry N,Stavroulakis S,Guan W,Davison GM,Bell HA,Weaver RJ,Down RE,Gatehouse JA,Gatehouse AM

更新日期：2011-05-01 00:00:00

abstract：:Brucella melitensis is a facultative intracellular bacterial pathogen that causes brucellosis, a zoonotic disease primarily infecting sheep and goats, characterized by undulant fever, arthritic pain and other neurological disorders in humans. A comprehensive proteomic study of strain 16M was conducted to identify and ...

journal_title：Proteomics

authors： Wagner MA,Eschenbrenner M,Horn TA,Kraycer JA,Mujer CV,Hagius S,Elzer P,DelVecchio VG

更新日期：2002-08-01 00:00:00

abstract：:Ischemia/reperfusion (I/R) injury is a major consequence of a cardiovascular intervention. The study of changes of the left and right ventricle proteomes from hearts subjected to I/R may be a key to revealing the pathological mechanisms underlying I/R-induced heart contractile dysfunction. Isolated rat hearts were per...

journal_title：Proteomics

authors： Cadete VJ,Lin HB,Sawicka J,Wozniak M,Sawicki G

更新日期：2012-08-01 00:00:00

abstract：:Long-term storage of protein samples for transportation is a challenge in the field of mass spectrometric analysis because the low temperature condition is not easy to be maintained. Here we introduce a simple method to preserve proteins at room temperature for at least one month. In this method, the protein sample is...

journal_title：Proteomics

authors： Chen Y,Wang S,Jia J,Tian X,Xu H,Ning M,Bai B

更新日期：2018-07-01 00:00:00

abstract：:Current treatments for advanced prostate cancer focus on inhibition of the androgen receptor (AR) by androgen deprivation therapy (ADT). However, complex interactions mediated by tumor suppressors, oncogenes, aberrations of AR expression, or de novo androgen production have been shown to induce the adaptive response o...

journal_title：Proteomics

authors： Soekmadji C,Rockstroh A,Ramm GA,Nelson CC,Russell PJ

更新日期：2017-12-01 00:00:00

abstract：:Bacillus subtilis is a prolific producer of enzymes and biopharmaceuticals. However, the susceptibility of heterologous proteins to degradation by (extracellular) proteases is a major limitation for use of B. subtilis as a protein cell factory. An increase in protein production levels has previously been achieved by u...

journal_title：Proteomics

authors： Westers L,Westers H,Zanen G,Antelmann H,Hecker M,Noone D,Devine KM,van Dijl JM,Quax WJ

更新日期：2008-07-01 00:00:00

abstract：:In this study, a method, based on dual column affinity chromatography hyphenated to isotope dilution inductively coupled plasma-quadrupole MS, was developed for selenium determination in selenoprotein P, glutathione peroxidase, and selenoalbumin in human serum samples from a group of healthy volunteers (n=399). Method...

journal_title：Proteomics

authors： Letsiou S,Lu Y,Nomikos T,Antonopoulou S,Panagiotakos D,Pitsavos C,Stefanadis C,Pergantis SA

更新日期：2010-10-01 00:00:00