OMSSAGUI: An open-source user interface component to configure and run the OMSSA search engine.

abstract：:Knowledge of gender differences is important because nutritional recommendations on the basis of data collected using predominantly male subjects may not be valid for women. In the present study, we performed proteomic analysis in plasma of rats fed a high-fat diet (HFD) using 2-DE combined with MALDI-TOF-MS for analy...

journal_title：Proteomics

authors： Liu H,Choi JW,Yun JW

更新日期：2012-01-01 00:00:00

abstract：:Radiation therapy (RT), combined with chemotherapy, is currently the standard adjuvant approach for UICC-stage II and III rectal cancer patients. Individual rectal tumors display wide ranges of radiosensitivity (RS). The aim of the present study was to identify proteins associated with radioresistance (RR), with the f...

journal_title：Proteomics

authors： Allal AS,Kähne T,Reverdin AK,Lippert H,Schlegel W,Reymond MA

更新日期：2004-08-01 00:00:00

abstract：:Proteins undergo PTM, which modulates their structure and regulates their function. Estimates of the PTM occurrence vary but it is safe to assume that there is an important gap between what is currently known and what remains to be discovered. The highest throughput and most comprehensive efforts to catalogue protein ...

journal_title：Proteomics

authors： Ahrné E,Müller M,Lisacek F

更新日期：2010-02-01 00:00:00

abstract：:Oligomeric ubiquitin structures (i.e. ubiquitin "chains") may be formed through any of seven different lysine residues in the polypeptide, or via the amine group of Met 1. Different types of ubiquitin chains can confer very different biological outcomes to a protein substrate, yet the structural characteristics of E2s...

journal_title：Proteomics

authors： Hong JH,Ng D,Srikumar T,Raught B

更新日期：2015-09-01 00:00:00

abstract：:Ruminants make large contributions to sustainable agriculture by converting crop by-products into agricultural food. Multi-omics integrative analysis helps to uncover the underlying molecular mechanisms. The liver metabolome-transcriptome interface (LMTI) in dairy cows, including 3938 significant correlations (p < 0.0...

journal_title：Proteomics

authors： Sun HZ,Wang DM,Liu HY,Liu JX

更新日期：2018-08-01 00:00:00

abstract：:The aim of the study was to evaluate the SELDI-TOF MS technique for pancreatic islet research. Mouse islets were cultured at low or high glucose levels in the absence or presence of oleate and characterized by measuring insulin secretion and oxygen tension. Subsequently, the islets were protein profiled. Up to 200 dif...

journal_title：Proteomics

authors： Ortsäter H,Sundsten T,Lin JM,Bergsten P

更新日期：2007-09-01 00:00:00

abstract：:Many of the emerging technologies for the global evaluation of gene expression, at both the RNA and protein level, are being applied to the problem of finding biomarkers for human disease progression. These analyses can be made difficult, however, by variation between samples that arises from both technical and nondis...

journal_title：Proteomics

authors： Steel LF,Shumpert D,Trotter M,Seeholzer SH,Evans AA,London WT,Dwek R,Block TM

更新日期：2003-05-01 00:00:00

abstract：:We have developed a surface plasmon resonance (SPR)-based protein microarray to study protein-protein interactions in a high-throughput mode. As a model system, triple protein interactions have been explored with human papillomaviral E6 protein, tumor suppressor p53, and ubiquitin ligase E6AP. Human papillomavirus (HP...

journal_title：Proteomics

authors： Ro HS,Koh BH,Jung SO,Park HK,Shin YB,Kim MG,Chung BH

更新日期：2006-04-01 00:00:00

abstract：:The 26S proteasome is a multicatalytic protease complex that degrades ubiquitinated proteins in eukaryotic cells. It consists of a proteolytic core (the 20S proteasome) as well as regulatory particles, which contain six ATPase (Rpt) subunits involved in unfolding and translocation of substrates to the catalytic chambe...

journal_title：Proteomics

authors： Kimura Y,Kurata Y,Ishikawa A,Okayama A,Kamita M,Hirano H

更新日期：2013-11-01 00:00:00

abstract：:The severe pulmonary form of leptospirosis (SPFL) is an especially serious and rapid disease process characterized by alveolar hemorrhage and acute respiratory failure. The outer membrane of Leptospira facilitates direct interactions with the environs and likely contains important constituents involved during infectio...

journal_title：Proteomics

authors： Nally JE,Whitelegge JP,Aguilera R,Pereira MM,Blanco DR,Lovett MA

更新日期：2005-01-01 00:00:00

abstract：:In proteomic research, two-dimensional electrophoresis (2-D) is an important tool for investigating differential patterns of qualitative and quantitative protein expression. The strength of the technique is due to its unrivalled power of being able to separate simultaneously thousands of proteins. The key to the compa...

journal_title：Proteomics

authors： Veeser S,Dunn MJ,Yang GZ

更新日期：2001-07-01 00:00:00

abstract：:The determination of possible biomarkers in nasal secretion of healthy subjects can have a role in early diagnosis of diseases such as rhinosinusitis. For this purpose, nasal lavage fluids (NLFs) from ten volunteers, collected before and after they had been submitted to nasal provocations, were investigated. Separatio...

journal_title：Proteomics

authors： Casado B,Pannell LK,Iadarola P,Baraniuk JN

更新日期：2005-07-01 00:00:00

abstract：:The ZNF198-fibroblast growth factor receptor-1 (FGFR1) fusion kinase is a constitutively activated tyrosine kinase associated with a specific atypical myeloproliferative disease. The chimeric protein localizes to the cytoplasm, unlike the wild type FGFR1 receptor kinase, and presumably inappropriately phosphorylates s...

journal_title：Proteomics

authors： Kasyapa C,Gu TL,Nagarajan L,Polakiewicz R,Cowell JK

更新日期：2009-08-01 00:00:00

abstract：:Elite rice hybrids, when compared to their parental lines, exhibit increased yield and other favorable agronomical traits, such as pathogen- and water-stress resistances, which are described as heterosis, and the molecular mechanism of heterosis remains to be elucidated. Since genomic sequences of the paternal (9311) ...

journal_title：Proteomics

authors： Wang W,Meng B,Ge X,Song S,Yang Y,Yu X,Wang L,Hu S,Liu S,Yu J

更新日期：2008-11-01 00:00:00

abstract：:Two-dimensional remains one of the main experimental approaches in proteome analysis. However, comigration of protein leads to several limitations: lack of accuracy in protein identification, impaired comparative quantification, and PTM detection. We have optimized a third additional step of in-gel separation to allev...

journal_title：Proteomics

authors： Colignon B,Raes M,Dieu M,Delaive E,Mauro S

更新日期：2013-07-01 00:00:00

abstract：:Cofilin, an actin-binding protein, regulates the rate, nature and extent of assembly of the actin cytoskeleton. Native Phast gels show that the addition of cofilin to an actin-DNase I complex (74 kDa) results in the formation of a ternary complex of 94 kDa indicating an equimolar stoichiometry in the ternary complex. ...

journal_title：Proteomics

authors： Nosworthy NJ,Kekic M,dos Remedios CG

更新日期：2001-12-01 00:00:00

abstract：:To develop a standard method for separating highly basic proteins in mammalian cells, we established a 2-D LC separation system coupled with chromatofocusing/nonporous RP column chromatography (CF/NPRPC) in a ProteomeLab PF2D system. After standardizing conditions for 2-D LC, a 2-D liquid protein map of uninfected mac...

journal_title：Proteomics

authors： Shin YK,Lee HJ,Lee JS,Paik YK

更新日期：2006-02-01 00:00:00

abstract：:Proteomic analysis on sperm has been restricted to only a few model organisms. We present here a 2DE PAGE proteome map of sperm cells from a nonmodel organism, the marine mussel Mytilus edulis, a free-spawning marine invertebrate with external fertilization. Ninety-six protein spots showing high expression were select...

journal_title：Proteomics

authors： Diz AP,Dudley E,Skibinski DO

更新日期：2012-06-01 00:00:00

abstract：:The Asia Oceania Human Proteome Organisation (AOHUPO) has embarked on a Membrane Proteomics Initiative with goals of systematic comparison of strategies for analysis of membrane proteomes and discovery of membrane proteins. This multilaboratory project is based on the analysis of a subcellular fraction from mouse live...

journal_title：Proteomics

authors： Peng L,Kapp EA,Fenyö D,Kwon MS,Jiang P,Wu S,Jiang Y,Aguilar MI,Ahmed N,Baker MS,Cai Z,Chen YJ,Van Chi P,Chung MC,He F,Len AC,Liao PC,Nakamura K,Ngai SM,Paik YK,Pan TL,Poon TC,Salekdeh GH,Simpson RJ,Sirdesh

更新日期：2010-11-01 00:00:00

abstract：:An aim of proteome research is to identify the entire complement of proteins expressed in defined cell types of humans, animals, plants, and microorganisms. The approach requires searching for low abundant or even rarely expressed proteins in many cell types, as well as the determination of the protein expression leve...

journal_title：Proteomics

authors： Wildgruber R,Weber G,Wise P,Grimm D,Bauer J

更新日期：2014-03-01 00:00:00

abstract：:The phosphorylation of heat shock protein 27 (HSP27) occurs differently in human renal cell carcinoma (RCC) compared to homologous normal kidney tissue. Two-dimensional electrophoresis was used to separate and visualize HSP27, via immunostaining with anti-HSP27 antibody, in tumor and normal renal samples, obtained aft...

journal_title：Proteomics

authors： Tremolada L,Magni F,Valsecchi C,Sarto C,Mocarelli P,Perego R,Cordani N,Favini P,Galli Kienle M,Sanchez JC,Hochstrasser DF,Corthals GL

更新日期：2005-02-01 00:00:00

abstract：:Proteome analysis by two-dimensional gel electrophoresis (2-DE) together with mass spectrometry was applied to screen acute phase response (APR)-related proteins with low molecular weight in loach skin following injury. Furthermore, Western blotting and function tests were applied to confirm the results obtained from ...

journal_title：Proteomics

authors： Peng X,Wu Y,Chen J,Wang S

更新日期：2004-12-01 00:00:00

abstract：:The accurate and precise absolute abundance of proteins can be determined using mass spectrometry by spiking the sample with stable isotope-labeled standards. In this study, we developed a strategy of hierarchical use of peptide-concatenated standards (PCSs) to quantify more proteins over a wider dynamic range. Multip...

journal_title：Proteomics

authors： Kito K,Okada M,Ishibashi Y,Okada S,Ito T

更新日期：2016-05-01 00:00:00

abstract：:Corynebacteria belong to the high G+C Gram-positive bacteria (Actinobacteria) and are closely related to Mycobacterium and Nocardia species. The best investigated member of this group of almost seventy species is Corynebacterium glutamicum, a soil bacterium isolated in 1957, which is used for the industrial production...

journal_title：Proteomics

authors： Poetsch A,Haussmann U,Burkovski A

更新日期：2011-08-01 00:00:00

abstract：:Tectona grandis L. (teak) is one of the premier hardwood timbers in the world, ranking at present in the top five tropical hardwood species in terms of worldwide plantation area. Characterization of the proteins present in teak leaves will provide a basis for the development of new tools aimed at assisting tree select...

journal_title：Proteomics

authors： Quiala E,Cañal MJ,Rodríguez R,Yagüe N,Chávez M,Barbón R,Valledor L

更新日期：2012-04-01 00:00:00

abstract：:Normal sera contain a large number of naturally occurring autoantibodies which can mask important disease-associated ones. Western blotting has evolved as the most important tool to demonstrate autoantibodies in autoimmune diseases, because of its ability to simultaneous screening for a wide spectrum of different anti...

journal_title：Proteomics

authors： Grus FH,Joachim SC,Pfeiffer N

更新日期：2003-06-01 00:00:00

abstract：:Employing classical two-dimensional electrophoresis (2-DE), amino acid sequencing and immunoblot analysis, we examine for the first time the effect of ozone, a highly notorious environmental pollutant, on rice seedling proteins. Drastic visible necrotic damage to leaf by ozone and consequent increase in ascorbate pero...

journal_title：Proteomics

authors： Agrawal GK,Rakwal R,Yonekura M,Kubo A,Saji H

更新日期：2002-08-01 00:00:00

abstract：:MS protein identification and quantitation are key proteomic techniques in biological research. Besides identification of proteins, MS is used increasingly to characterize secondary protein modifications. This often requires trimming the analytical strategy to a specific type of modification. Direct analysis of protei...

journal_title：Proteomics

authors： Niggeweg R,Köcher T,Gentzel M,Buscaino A,Taipale M,Akhtar A,Wilm M

更新日期：2006-01-01 00:00:00

abstract：:Precise protein quantification is essential in comparative proteomics. Currently, quantification bias is inevitable when using proteotypic peptide-based quantitative proteomics strategy for the differences in peptides measurability. To improve quantification accuracy, we proposed an "empirical rule for linearly correl...

journal_title：Proteomics

authors： Liu K,Zhang J,Fu B,Xie H,Wang Y,Qian X

更新日期：2014-07-01 00:00:00

abstract：:Arabidopsis thaliana cell suspension cultures have been used to investigate the effects of salinity and hyperosmotic stress on plant cellular proteins. We show that 200 mM NaCl and 400 mM sorbitol treatments induce extracellular medium acidification in Arabidopsis cell cultures, a typical response of plant cells to sa...

journal_title：Proteomics

authors： Ndimba BK,Chivasa S,Simon WJ,Slabas AR

更新日期：2005-11-01 00:00:00