Stable Protein Gel Storage in Acetonitrile for Mass Spectrometric Analysis.


:Long-term storage of protein samples for transportation is a challenge in the field of mass spectrometric analysis because the low temperature condition is not easy to be maintained. Here we introduce a simple method to preserve proteins at room temperature for at least one month. In this method, the protein sample is run shortly into a polyacrylamide gel which is then excised after Coomassie staining. The protein gel band is then dehydrated by 100% acetonitrile three times and kept in 100% acetonitrile for storage at room temperature. By the TMT 10-plex based quantitative proteomic analyses, we have found that these proteins are stable in their levels and modifications (phosphorylation, oxidation, and ubiquitination) for 30 days. Further analysis has revealed this storage method also well preserves proteins even at 45 °C. We therefore recommend to use acetonitrile to dehydrate and store protein gel pieces as an effective alternative for sample shipping over days. Therefore, it might facilitate worldwide collaborations in the mass spectrometry-based proteomic research.






Chen Y,Wang S,Jia J,Tian X,Xu H,Ning M,Bai B




Has Abstract


2018-07-01 00:00:00












  • An approach to remove albumin for the proteomic analysis of low abundance biomarkers in human serum.

    abstract::Proteomic technologies are being used to discover and identify disease-associated biomarkers. The application of these technologies in the search for potential diagnostic/prognostic biomarkers in the serum of patients has been limited by the presence of highly abundant albumin and immunoglobulins that constitute appro...


    pub_type: 杂志文章


    authors: Ahmed N,Barker G,Oliva K,Garfin D,Talmadge K,Georgiou H,Quinn M,Rice G

    更新日期:2003-10-01 00:00:00

  • Proteomic expression analysis of colorectal cancer by two-dimensional differential gel electrophoresis.

    abstract::The identification of specific protein markers for colorectal cancer would provide the basis for early diagnosis and detection, as well as clues for understanding the molecular mechanisms governing cancer progression. In this report, we describe the proteomic analysis of the samples of colorectal cancer corresponding ...


    pub_type: 杂志文章


    authors: Alfonso P,Núñez A,Madoz-Gurpide J,Lombardia L,Sánchez L,Casal JI

    更新日期:2005-07-01 00:00:00

  • A strategy for the comparative analysis of serum proteomes for the discovery of biomarkers for hepatocellular carcinoma.

    abstract::Many of the emerging technologies for the global evaluation of gene expression, at both the RNA and protein level, are being applied to the problem of finding biomarkers for human disease progression. These analyses can be made difficult, however, by variation between samples that arises from both technical and nondis...


    pub_type: 杂志文章


    authors: Steel LF,Shumpert D,Trotter M,Seeholzer SH,Evans AA,London WT,Dwek R,Block TM

    更新日期:2003-05-01 00:00:00

  • DNA-Barcoded Fluorescence Microscopy for Spatial Omics.

    abstract::Innovation in genomics, transcriptomics, and proteomics research has created a plethora of state-of-the-art techniques such as nucleic acid sequencing and mass-spectrometry-based proteomics with paramount impact in the life sciences. While current approaches yield quantitative abundance analysis of biomolecules on an ...


    pub_type: 杂志文章,评审


    authors: Schueder F,Unterauer EM,Ganji M,Jungmann R

    更新日期:2020-10-08 00:00:00

  • Differential expression of cardiac mitochondrial proteins.

    abstract::Mitochondria were isolated from whole hearts of Dahl salt sensitive (SS) and chromosome 13 consomic control (SS.13BN/Mcwi) rats using a mechanical homogenization process followed by density centrifugation. The proteins present in the two mitochondria preparations were quantified; equal amounts of protein from each sam...


    pub_type: 杂志文章


    authors: Smith JR,Matus IR,Beard DA,Greene AS

    更新日期:2008-02-01 00:00:00

  • Identification of Rabbit Oviductal Fluid Proteins Involved in Pre-Fertilization Processes by Quantitative Proteomics.

    abstract::Oviductal fluid (ODF) proteins modulate and support reproductive processes in the oviduct. In the present study, proteins involved in the biological events that precede fertilization have been identified in the rabbit ODF proteome, isolated from the ampulla and isthmus of the oviduct at different time points within 8 ...


    pub_type: 杂志文章


    authors: Yu H,Hackenbroch L,Meyer FRL,Reiser J,Razzazi-Fazeli E,Nöbauer K,Besenfelder U,Vogl C,Brem G,Mayrhofer C

    更新日期:2019-03-01 00:00:00

  • The post-genomic era of interactive proteomics: facts and perspectives.

    abstract::The availability of completed genome sequences of several eukaryotic and prokaryotic species has shifted the focus towards the identification and characterization of all gene products that are expressed in a given organism. In order to cope with the huge amounts of data that have been provided by large-scale sequencin...


    pub_type: 杂志文章,评审


    authors: Auerbach D,Thaminy S,Hottiger MO,Stagljar I

    更新日期:2002-06-01 00:00:00

  • 2-D DIGE analysis of the proteome of extracts from peanut variants reveals striking differences in major allergen contents.

    abstract::Over the last decade, an increasing prevalence of peanut allergies was observed worldwide. Peanuts are meanwhile categorized among the most dangerous food allergens. This is particularly relevant since peanut-derived ingredients are widely used in industrial food production. To minimize the problem of hidden food alle...


    pub_type: 杂志文章


    authors: Schmidt H,Gelhaus C,Latendorf T,Nebendahl M,Petersen A,Krause S,Leippe M,Becker WM,Janssen O

    更新日期:2009-07-01 00:00:00

  • Proteomic Profile of Daphnia pulex using Data-Independent Acquisition Mass Spectrometry and Ion Mobility Separation.

    abstract::Daphnia pulex is a keystone species for aquatic habitats and an ecological/evolution model organism. Although significant progress has been made on characterizing its genome, the D. pulex proteome remains largely uncharacterized partially due to abnormally high protein degradation during homogenization and emphasis on...


    pub_type: 杂志文章


    authors: Steevensz A,Gombar R,Vergilino R,Cristescu ME,Vacratsis PO

    更新日期:2018-08-01 00:00:00

  • Transfer and multiplex immunoblotting of a paraffin embedded tissue.

    abstract::As we transition from genomics to the challenges of the functional proteome, new tools to explore the expression of proteins within tissue are essential. We have developed a method of transferring proteins from a formalin fixed, paraffin embedded tissues section to a stack of membranes which is then probed with antibo...


    pub_type: 杂志文章


    authors: Chung JY,Braunschweig T,Baibakov G,Galperin M,Ramesh A,Skacel M,Gannot G,Knezevic V,Hewitt SM

    更新日期:2006-02-01 00:00:00

  • CD28 stimulation regulates its association with N-ethylmaleimide-sensitive fusion protein and other proteins involved in vesicle sorting.

    abstract::CD28 delivers a co-stimulatory signal for T cell antigen receptor induced activation of T cells through a mechanism which remains mostly elusive to date. In order to try and gain insight into CD28 function, we therefore applied state-of-the-art mass spectrometric protein identification technology to the analysis of CD...


    pub_type: 杂志文章


    authors: Heller M,Watts JD,Aebersold R

    更新日期:2001-01-01 00:00:00

  • 6th HUPO Annual World Congress - Proteomics Standards Initiative Workshop 6-10 October 2007, Seoul, Korea.

    abstract::The plenary session of the Proteomics Standards Initiative of the Human Proteome Organisation at the 6th annual HUPO world congress updated the delegates on the current status of the ongoing work of this group. The new MS interchange format, mzML, had been entered into the HUPO document process immediately prior to th...




    authors: Orchard S,Martens L,Tasman J,Binz PA,Albar JP,Hermjakob H

    更新日期:2008-04-01 00:00:00

  • 2-D DIGE analysis of the mitochondrial proteome from human skeletal muscle reveals time course-dependent remodelling in response to 14 consecutive days of endurance exercise training.

    abstract::Adaptation of skeletal muscle to repeated bouts of endurance exercise increases aerobic capacity and improves mitochondrial function. However, the adaptation of human skeletal muscle mitochondrial proteome to short-term endurance exercise training has not been investigated. Eight sedentary males cycled for 60 min at 8...


    pub_type: 杂志文章


    authors: Egan B,Dowling P,O'Connor PL,Henry M,Meleady P,Zierath JR,O'Gorman DJ

    更新日期:2011-04-01 00:00:00

  • Catch me if you can: mass spectrometry-based phosphoproteomics and quantification strategies.

    abstract::Phosphorylation of proteins is one of the most prominent PTMs and for instance a key regulator of signal transduction. In order to improve our understanding of cellular phosphorylation events, considerable effort has been devoted to improving the analysis of phosphorylation by MS-based proteomics. Different enrichment...


    pub_type: 杂志文章,评审


    authors: Eyrich B,Sickmann A,Zahedi RP

    更新日期:2011-02-01 00:00:00

  • In vivo trapping of FtsH substrates by label-free quantitative proteomics.

    abstract::FtsH is the only membrane-bound and essential protease in Escherichia coli. It is responsible for the degradation of regulatory proteins and enzymes such as the heat-shock sigma factor RpoH or LpxC, the key enzyme of lipopolysaccharide biosynthesis. To find new FtsH targets, we trapped substrates in E. coli cells from...


    pub_type: 杂志文章


    authors: Arends J,Thomanek N,Kuhlmann K,Marcus K,Narberhaus F

    更新日期:2016-12-01 00:00:00

  • Evaluation and optimization of IgY spin column technology in the depletion of abundant proteins from human serum.

    abstract::Serum depletion strategies are commonly implemented in order to remove abundant proteins, increasing the number of proteins detected in a biomarker study. The IgY spin columns used in this study bind 12 and 14 primate proteins, respectively. 1-D SDS-PAGE and 2-DE revealed a suboptimal performance of the IgY spin colum...


    pub_type: 杂志文章


    authors: Corrigan L,Jefferies C,Clive Lee T,Daly J

    更新日期:2011-08-01 00:00:00

  • Numerical approaches for quantitative analysis of two-dimensional maps: a review of commercial software and home-made systems.

    abstract::The present review attempts to cover a number of methods that have appeared in the last few years for performing quantitative proteome analysis. However, due to the large number of methods described for both electrophoretic and chromatographic approaches, we have limited this review to conventional two-dimensional (2-...


    pub_type: 杂志文章,评审


    authors: Marengo E,Robotti E,Antonucci F,Cecconi D,Campostrini N,Righetti PG

    更新日期:2005-02-01 00:00:00

  • Predicting adverse drug reaction profiles by integrating protein interaction networks with drug structures.

    abstract::The prediction of adverse drug reactions (ADRs) has become increasingly important, due to the rising concern on serious ADRs that can cause drugs to fail to reach or stay in the market. We proposed a framework for predicting ADR profiles by integrating protein-protein interaction (PPI) networks with drug structures. W...


    pub_type: 杂志文章


    authors: Huang LC,Wu X,Chen JY

    更新日期:2013-01-01 00:00:00

  • Gel-free analysis of the human brain proteome: application of liquid chromatography and mass spectrometry on biopsy and autopsy samples.

    abstract::This paper reports on the findings of the Biomedical Research Institute, as one of the participants in the pilot study of the HUPO Brain Proteome Project. A biopsy and autopsy study sample derived from human brain was distributed among the participants for proteomic analysis. In our laboratory, attention was focused o...


    pub_type: 杂志文章


    authors: Dumont D,Noben JP,Verhaert P,Stinissen P,Robben J

    更新日期:2006-09-01 00:00:00

  • Proteomic profiling of extracellular vesicles secreted from Toxoplasma gondii.

    abstract::Toxoplasma gondii infects a wide range of hosts worldwide, including humans and domesticated animals causing toxoplasmosis disease. Recently, exosomes, small extracellular vesicles (EV) that contain nucleic acids, proteins, and lipids derived from their original cells were linked with disease protection. The effect of...


    pub_type: 杂志文章


    authors: Wowk PF,Zardo ML,Miot HT,Goldenberg S,Carvalho PC,Mörking PA

    更新日期:2017-08-01 00:00:00

  • The cell wall subproteome of Listeria monocytogenes.

    abstract::The surface subproteome of Listeria monocytogenes that includes many proteins already known to be involved in virulence and interaction with host cells has been characterized. A new method for the isolation of a defined surface proteome of low complexity has been established based on serial extraction of proteins by d...


    pub_type: 杂志文章


    authors: Schaumburg J,Diekmann O,Hagendorff P,Bergmann S,Rohde M,Hammerschmidt S,Jänsch L,Wehland J,Kärst U

    更新日期:2004-10-01 00:00:00

  • Advancing the sensitivity of selected reaction monitoring-based targeted quantitative proteomics.

    abstract::Selected reaction monitoring (SRM) - also known as multiple reaction monitoring (MRM) - has emerged as a promising high-throughput targeted protein quantification technology for candidate biomarker verification and systems biology applications. A major bottleneck for current SRM technology, however, is insufficient se...


    pub_type: 杂志文章,评审


    authors: Shi T,Su D,Liu T,Tang K,Camp DG 2nd,Qian WJ,Smith RD

    更新日期:2012-04-01 00:00:00

  • Gas phase conformation can have an influence on peptide fragmentation.

    abstract::In the post-genomic era, mass spectrometry is destined to fulfil a central role in biomedical research, and it is in the area of protein identification that mass spectrometry is now most rapidly expanding. An important identification method is to subject a protein to proteolysis and determine the resulting peptide mas...


    pub_type: 杂志文章


    authors: Griffiths WJ,Jonsson AP

    更新日期:2001-08-01 00:00:00

  • Quantitative proteomics reveals differentially expressed proteins in murine preneoplastic intestine in a model of intestinal tumorigenesis induced by low dietary folate and MTHFR deficiency.

    abstract::Colorectal cancer risk is increased when dietary folate intake is low, with or without a deficiency in methylenetetrahydrofolate reductase (MTHFR). We have observed that intestinal tumors are induced in mice fed low-folate diets, and that tumor incidence is increased when these mice also have MTHFR deficiency. This st...


    pub_type: 杂志文章


    authors: Leclerc D,Dejgaard K,Mazur A,Deng L,Wu Q,Nilsson T,Rozen R

    更新日期:2014-11-01 00:00:00

  • Proteomic analysis of membrane microdomains derived from both failing and non-failing human hearts.

    abstract::Eukaryotic cells plasma membranes are organized into microdomains of specialized function such as lipid rafts and caveolae, with a specific lipid composition highly enriched in cholesterol and glycosphingolipids. In addition to their role in regulating signal transduction, multiple functions have been proposed, such a...


    pub_type: 杂志文章


    authors: Banfi C,Brioschi M,Wait R,Begum S,Gianazza E,Fratto P,Polvani G,Vitali E,Parolari A,Mussoni L,Tremoli E

    更新日期:2006-03-01 00:00:00

  • Quantitative phosphoproteomics of Alzheimer's disease reveals cross-talk between kinases and small heat shock proteins.

    abstract::Abnormal phosphorylation contributes to the formation of neurofibrillary tangles in Alzheimer's disease (AD), but may play other signaling roles during AD pathogenesis. In this study, we employed IMAC followed by LC-MS/MS to identify phosphopeptides from eight individual AD and eight age-matched control postmortem hum...


    pub_type: 杂志文章


    authors: Dammer EB,Lee AK,Duong DM,Gearing M,Lah JJ,Levey AI,Seyfried NT

    更新日期:2015-01-01 00:00:00

  • Toward Axonal System Biology: Genome Wide Views of Local mRNA Translation.

    abstract::Neurons present a highly polarized morphology, often displaying a significantly imbalanced distribution of the cytoplasm between the somatic and axonal domains. This imbalance requires cell-specific mechanisms for the maintenance of the axoplasmic mass during development, neuronal homeostasis, and recovery after injur...


    pub_type: 杂志文章,评审


    authors: Farias J,Sotelo JR,Sotelo-Silveira J

    更新日期:2019-08-01 00:00:00

  • Protein profile in neuroblastoma cells incubated with S- and R-enantiomers of ibuprofen by iTRAQ-coupled 2-D LC-MS/MS analysis: possible action of induced proteins on Alzheimer's disease.

    abstract::Ibuprofen is a member of the proprionic acid group of nonsteroidal anti-inflammatory drugs (NSAID), with the S-enantiomer being more active than the R-enantiomer. It has been shown to display protective effects against neuroinflammation, which is linked to the pathogenesis of several neurodegenerative disorders, inclu...


    pub_type: 杂志文章


    authors: Zhang J,Sui J,Ching CB,Chen WN

    更新日期:2008-04-01 00:00:00

  • Proteomic analysis of bovine skeletal muscle hypertrophy.

    abstract::Myostatin plays a major role in muscle growth and development and animals with disruption of this gene display marked increases in muscle mass. Little is known about muscle physiological adaptations in relation to this muscle hypertrophy. To provide a more comprehensive view, we analyzed bovine muscles from control, h...


    pub_type: 杂志文章


    authors: Bouley J,Meunier B,Chambon C,De Smet S,Hocquette JF,Picard B

    更新日期:2005-02-01 00:00:00

  • RNAi Transfection Results in Lipidome Changes.

    abstract::RNAi experiments are ubiquitously used in cell biology and are achieved by transfection of small interfering RNAs (siRNAs) into cells using a transfection reagent. These results in knock-down of proteins of interest, and the phenotypic consequences are then analyzed. It is reported here that two common RNA interferenc...


    pub_type: 杂志文章


    authors: Özbalci C,Storck EM,Eggert US

    更新日期:2019-07-01 00:00:00