Separation of human erythrocyte membrane associated proteins with one-dimensional and two-dimensional gel electrophoresis followed by identification with matrix-assisted laser desorption/ionization-time of flight mass spectrometry.

abstract：:Topoisomerase (topo) II catalyzes topological changes in DNA. Although both human isozymes, topo IIα and β are phosphorylated, site-specific phosphorylation of topo IIβ is poorly characterized. Using LC-MS/MS analysis of topo IIβ, cleaved with trypsin, Arg C or cyanogen bromide (CNBr) plus trypsin, we detected four +8...

journal_title：Proteomics

authors： Grozav AG,Willard BB,Kozuki T,Chikamori K,Micluta MA,Petrescu AJ,Kinter M,Ganapathi R,Ganapathi MK

更新日期：2011-03-01 00:00:00

abstract：:Tectona grandis L. (teak) is one of the premier hardwood timbers in the world, ranking at present in the top five tropical hardwood species in terms of worldwide plantation area. Characterization of the proteins present in teak leaves will provide a basis for the development of new tools aimed at assisting tree select...

journal_title：Proteomics

authors： Quiala E,Cañal MJ,Rodríguez R,Yagüe N,Chávez M,Barbón R,Valledor L

更新日期：2012-04-01 00:00:00

abstract：:Precise protein quantification is essential in comparative proteomics. Currently, quantification bias is inevitable when using proteotypic peptide-based quantitative proteomics strategy for the differences in peptides measurability. To improve quantification accuracy, we proposed an "empirical rule for linearly correl...

journal_title：Proteomics

authors： Liu K,Zhang J,Fu B,Xie H,Wang Y,Qian X

更新日期：2014-07-01 00:00:00

abstract：:Sepsis is an extreme host response to infection that leads to loss of organ function and cardiovascular integrity. Mortality from sepsis is on the rise. Despite more than three decades of research and clinical trials, specific diagnostic and therapeutic strategies for sepsis are still absent. The use of LFQ- and TMT-b...

journal_title：Proteomics

authors： Pimienta G,Heithoff DM,Rosa-Campos A,Tran M,Esko JD,Mahan MJ,Marth JD,Smith JW

更新日期：2019-03-01 00:00:00

abstract：:Over the last decade, an increasing prevalence of peanut allergies was observed worldwide. Peanuts are meanwhile categorized among the most dangerous food allergens. This is particularly relevant since peanut-derived ingredients are widely used in industrial food production. To minimize the problem of hidden food alle...

journal_title：Proteomics

authors： Schmidt H,Gelhaus C,Latendorf T,Nebendahl M,Petersen A,Krause S,Leippe M,Becker WM,Janssen O

更新日期：2009-07-01 00:00:00

abstract：:Reporter ion-based methods are among the major techniques to quantify peptides and proteins. Two main labels, tandem mass tag (TMT) and iTRAQ, are widely used by the proteomics community. They are, however, often applied as out-of-the-box methods, without thorough quality control. Thus, due to undiscovered limitations...

journal_title：Proteomics

authors： Burkhart JM,Vaudel M,Zahedi RP,Martens L,Sickmann A

更新日期：2011-03-01 00:00:00

abstract：:Quantitative proteomics is the comparison of distinct proteomes which enables the identification of protein species which exhibit changes in expression or post-translational state in response to a given stimulus. Many different quantitative techniques are being utilized and generate large datasets. Independent of the ...

journal_title：Proteomics

authors： Karp NA,Lilley KS

更新日期：2007-09-01 00:00:00

abstract：:Integral membrane proteins (IMPs) perform crucial cellular functions and are the primary targets for most pharmaceutical agents. However, the hydrophobic nature of their membrane-embedded domains and their intimate association with lipids make them difficult to handle. Numerous proteomic platforms that include LC sepa...

journal_title：Proteomics

authors： Blackler AR,Speers AE,Wu CC

更新日期：2008-10-01 00:00:00

abstract：:Menthol is a naturally occurring cyclic monoterpene used in oral hygiene products, confectionary, pharmaceuticals, cosmetics, pesticides, and as a flavoring agent. In the present study, we analyzed the differentially expressing proteome in L-menthol-treated Caco-2 cell line as it was found to inhibit cell proliferatio...

journal_title：Proteomics

authors： Faridi U,Sisodia BS,Shukla AK,Shukla RK,Darokar MP,Dwivedi UN,Shasany AK

更新日期：2011-05-01 00:00:00

abstract：:Eukaryotic lipid rafts are membrane microdomains that have significant amounts of cholesterol and a selective set of proteins that have been associated with multiple biological functions. The Lyme disease agent, Borrelia burgdorferi, is one of an increasing number of bacterial pathogens that incorporates cholesterol o...

journal_title：Proteomics

authors： Toledo A,Pérez A,Coleman JL,Benach JL

更新日期：2015-11-01 00:00:00

abstract：:A method is introduced to evaluate protein concentrations using the height sum of all MALDI-MS peaks that unambiguously match theoretic tryptic peptide masses of the protein sought after. The method uses native chromatographic protein fractionation prior to digestion but does not require any depletion, labeling, deriv...

journal_title：Proteomics

authors： Bublitz R,Kreusch S,Ditze G,Schulze M,Cumme GA,Fischer C,Winter A,Hoppe H,Rhode H

更新日期：2006-07-01 00:00:00

abstract：:Blood plasma and serum are very useful samples for the detection, identification and quantitation of proteins associated with both health and disease. However, analysis of plasma and serum is a challenge because traces of interesting polypeptides and proteins can be dominated by the very high concentration of albumin ...

journal_title：Proteomics

authors： Baussant T,Bougueleret L,Johnson A,Rogers J,Menin L,Hall M,Aberg PM,Rose K

更新日期：2005-03-01 00:00:00

abstract：:The spermatozoon is an accessible cell which can be easily purified and therefore it is particularly well suited for proteomic analysis. It is also an extremely differentiated cell with very marked genetic, cellular, functional and chromatin changes as compared to other cells, and has profound implications for fertili...

journal_title：Proteomics

authors： Oliva R,de Mateo S,Estanyol JM

更新日期：2009-02-01 00:00:00

abstract：:The severe pulmonary form of leptospirosis (SPFL) is an especially serious and rapid disease process characterized by alveolar hemorrhage and acute respiratory failure. The outer membrane of Leptospira facilitates direct interactions with the environs and likely contains important constituents involved during infectio...

journal_title：Proteomics

authors： Nally JE,Whitelegge JP,Aguilera R,Pereira MM,Blanco DR,Lovett MA

更新日期：2005-01-01 00:00:00

abstract：:This is the first broad proteomic description of Gluconacetobacter diazotrophicus, an endophytic bacterium, responsible for the major fraction of the atmospheric nitrogen fixed in sugarcane in tropical regions. Proteomic coverage of G. diazotrophicus PAL5 was obtained by two independent approaches: 2-DE followed by MA...

journal_title：Proteomics

authors： Lery LM,Coelho A,von Kruger WM,Gonçalves MS,Santos MF,Valente RH,Santos EO,Rocha SL,Perales J,Domont GB,Teixeira KR,Bertalan M,Ferreira PC,Bisch PM

更新日期：2008-04-01 00:00:00

abstract：:Shewanella inhabit a wide variety of niches in nature and can utilize a broad spectrum of electron acceptors under anaerobic conditions. How they modulate their gene expression to adapt is poorly understood. ArcA, homologue of a global regulator controlling hundreds of genes involved in aerobic and anaerobic respirati...

journal_title：Proteomics

authors： Yuan J,Wei B,Lipton MS,Gao H

更新日期：2012-06-01 00:00:00

abstract：:The function of a protein is modulated by its abundance and its degree of specific post-translational modifications such as phosphorylation, glycosylation or truncation. Consequently, changes of protein concentration and the extent of their post-translational modifications has a great influence on the activity of intr...

journal_title：Proteomics

authors： Moritz B,Meyer HE

更新日期：2003-11-01 00:00:00

abstract：:We introduce a new methodology named ligand profiling and identification for effective discovery of bioactive ligands such as peptide hormones. This technology was developed from a new concept of parallel column chromatography and active fraction profiling by nano-LC MS. Traditional methods use sequential column chrom...

journal_title：Proteomics

authors： Baek MC,Kim SJ,Yea K,Kim Y,Lee BD,Kim J,Lee HJ,Kang MH,Choi SK,Kim JI,Lee TG,Suh PG,Ryu SH

更新日期：2006-03-01 00:00:00

abstract：:Heat shock proteins (HSPs) are crucial for the maintenance of cell integrity during normal cellular growth as well as during pathophysiological conditions. While functioning mainly as molecular chaperones, HSPs also appear to be involved in diverse biological activities, such as apoptosis, carcinogenesis, and cytoprot...

journal_title：Proteomics

authors： Yeo M,Park HK,Kim DK,Cho SW,Kim YS,Cho SY,Paik YK,Hahm KB

更新日期：2004-11-01 00:00:00

abstract：:Gliadins and glutenins are the main protein fractions present in wheat gluten. They are responsible for technological and nutritional quality of wheat based products. In particular, glutenins are mainly responsible for dough visco-elastic properties, whereas gliadins confer extensibility to dough and are the most impo...

journal_title：Proteomics

authors： Ferrante P,Masci S,D'Ovidio R,Lafiandra D,Volpi C,Mattei B

更新日期：2006-03-01 00:00:00

abstract：:Modification through beta-elimination has proven to be a reliable first step in the approach for enrichment of serine/threonine-phopshorylated (Ser-/Thr) peptides. However, under harsh basic conditions, Ser-/Thr-glycosylated peptides are susceptible to beta-elimination as well. Therefore, we have optimized these condi...

journal_title：Proteomics

authors： Poot AJ,Ruijter E,Nuijens T,Dirksen EH,Heck AJ,Slijper M,Rijkers DT,Liskamp RM

更新日期：2006-12-01 00:00:00

abstract：:Campylobacter jejuni is a major gastrointestinal pathogen that colonizes host mucosa via interactions with extracellular matrix proteins, such as fibronectin (Fn). Fn-binding is mediated by a 37 kDa outer membrane protein termed Campylobacter adherence Factor (CadF). The outer membrane protein profile of a recent gast...

journal_title：Proteomics

authors： Scott NE,Marzook NB,Deutscher A,Falconer L,Crossett B,Djordjevic SP,Cordwell SJ

更新日期：2010-01-01 00:00:00

abstract：:Experimental identification of expressed proteins by proteomics constitutes the most reliable approach to identify genomic location and structure of protein-coding genes and substantially complements computational genome annotation. Channel catfish herpesvirus (CCV) is a simple comparative model for understanding herp...

journal_title：Proteomics

authors： Kunec D,Nanduri B,Burgess SC

更新日期：2009-05-01 00:00:00

abstract：:In mass spectrometry-based proteomics, protein identification results usually consist of peptide sequences and database-dependent accession identifiers of the matching proteins. Often certain annotations are only available in particular databases that in turn must be queried by a certain identifier. In order to simpli...

journal_title：Proteomics

authors： Boehm AM,Sickmann A

更新日期：2006-08-01 00:00:00

abstract：:Mammalian cells secrete two types of extracellular vesicles either constitutively or in a regulated manner: exosomes (50-100 nm in diameter) released from the intracellular compartment and ectosomes (also called microvesicles, 100-1000 nm in diameter) shed directly from the plasma membrane. Extracellular vesicles are ...

journal_title：Proteomics

authors： Choi DS,Kim DK,Kim YK,Gho YS

更新日期：2013-05-01 00:00:00

abstract：:Affinity purification coupled to mass spectrometry (AP-MS) is gaining widespread use for the identification of protein-protein interactions. It is unclear, however, whether typical AP sample complexity is limiting for the identification of all protein components using standard one-dimensional LC-MS/MS. Multidimensiona...

journal_title：Proteomics

authors： Dunham WH,Larsen B,Tate S,Badillo BG,Goudreault M,Tehami Y,Kislinger T,Gingras AC

更新日期：2011-07-01 00:00:00

abstract：:As demonstrated in this study, a CIEF-based multidimensional separation platform not only is compatible with the detergent-based membrane protein preparation protocol, but also achieves both the largest yeast membrane proteome coverage and the most comprehensive analysis of the yeast proteome to date. By using a 1% fa...

journal_title：Proteomics

authors： Wang W,Guo T,Song T,Lee CS,Balgley BM

更新日期：2007-04-01 00:00:00

abstract：:The influenza virus polymerase is formed by the PB1, PB2 and PA subunits and is required for virus transcription and replication in the nucleus of infected cells. Here we present the characterisation of the complexes formed intracellularly by the influenza polymerase in human cells. The virus polymerase was expressed ...

journal_title：Proteomics

authors： Jorba N,Juarez S,Torreira E,Gastaminza P,Zamarreño N,Albar JP,Ortín J

更新日期：2008-05-01 00:00:00

abstract：:A novel proteolysis approach was developed by using infrared (IR) radiation and trypsin-immobilized silica microspheres. Protein solutions containing trypsin-immobilized microspheres in sealed transparent Eppendorf tubes were allowed to digest under an IR lamp at 37 degrees C. The feasibility and performance of the pr...

journal_title：Proteomics

authors： Bao H,Lui T,Zhang L,Chen G

更新日期：2009-02-01 00:00:00

abstract：:Prostate cancer is one of the leading cancers in men. Taking dietary supplements, such as fish oil (FO), which is rich in n-3 polyunsaturated fatty acids (PUFAs), has been employed as a strategy to lower prostate cancer risk and control disease progression. In this study, we investigated the global phosphoproteomic ch...

journal_title：Proteomics

authors： Zhao H,Pflug BR,Lai X,Wang M

更新日期：2016-09-01 00:00:00