Abstract:
:A novel proteolysis approach was developed by using infrared (IR) radiation and trypsin-immobilized silica microspheres. Protein solutions containing trypsin-immobilized microspheres in sealed transparent Eppendorf tubes were allowed to digest under an IR lamp at 37 degrees C. The feasibility and performance of the present proteolysis approach were demonstrated by the digestion of BSA and cytochrome c (Cyt-c) and the digestion time was significantly reduced to 5 min. The obtained digests were identified by MALDI-TOF-MS with the sequence coverages of 54% (BSA) and 83% (Cyt-c) that were better than those obtained by conventional in-solution tryptic digestion. The suitability of the new digestion approach to complex proteins was demonstrated by digesting human serum. The present proteolysis strategy is simple and efficient and will find a wide range of application in protein identification.
journal_name
Proteomicsjournal_title
Proteomicsauthors
Bao H,Lui T,Zhang L,Chen Gdoi
10.1002/pmic.200800659subject
Has Abstractpub_date
2009-02-01 00:00:00pages
1114-7issue
4eissn
1615-9853issn
1615-9861journal_volume
9pub_type
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