Abstract:
:Studying microbial interactions by MALDI mass spectrometry imaging (MSI) directly from growing media is a difficult task if high sensitivity is demanded. We present a quick and robust sample preparation strategy for growing fungi (Trichoderma atroviride, Rhizoctonia solani) on glass slides to establish a miniaturized confrontation assay. By this we were able to visualize metabolite distributions by MALDI MSI after matrix deposition with a home-built sublimation device and thorough recrystallization. We present for the first time MALDI MSI data for secondary metabolite release during active mycoparasitism.
journal_name
Proteomicsjournal_title
Proteomicsauthors
Holzlechner M,Reitschmidt S,Gruber S,Zeilinger S,Marchetti-Deschmann Mdoi
10.1002/pmic.201500510subject
Has Abstractpub_date
2016-06-01 00:00:00pages
1742-6issue
11-12eissn
1615-9853issn
1615-9861journal_volume
16pub_type
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