Abstract:
:An improved method for sample preparation for MALDI-MS and MS/MS using AnchorChip targets is presented. The method, termed the SMW method (sample, matrix wash), results in better sensitivity for peptide mass fingerprinting as well as for sequencing by MS/MS than previously published methods. The method allows up-concentration and desalting directly on the mass spectrometric target and should be amenable for automation. A draw back caused by extensive oxidation of methionine and tryptophan in the SMW method can be alleviated by the addition of n-octyl glucopyranoside and DTT to the sample solution. The method was validated for protein identification from a 2-DE based liver proteome study. The SMW method resulted in identification of many more proteins and in most cases with a better score than the previously published methods.
journal_name
Proteomicsjournal_title
Proteomicsauthors
Zhang X,Shi L,Shu S,Wang Y,Zhao K,Xu N,Liu S,Roepstorff Pdoi
10.1002/pmic.200600184subject
Has Abstractpub_date
2007-07-01 00:00:00pages
2340-9issue
14eissn
1615-9853issn
1615-9861journal_volume
7pub_type
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