Abstract:
:The problem with cancer tissue is that its intratumoral heterogeneity and its complexity is extremely high as cells possess, depending on their location and function, different mutations, different mRNA expression and the highest intricacy in the protein pattern. Prior to genomic and proteomic analyses, it is therefore indispensable to identify the exact part of the tissue or even the exact cell. Laser-based microdissection is a tried and tested technique able to produce pure and well-defined cell material for further analysis with proteomic and genomic techniques. It sheds light on the heterogeneity of cancer or other complex diseases and enables the identification of biomarkers. This review aims to raise awareness for the reconsideration of laser-based microdissection and seeks to present current state-of-the-art combinations with omic techniques.
journal_name
Proteomicsjournal_title
Proteomicsauthors
von Eggeling F,Hoffmann Fdoi
10.1002/pmic.202000077subject
Has Abstractpub_date
2020-09-01 00:00:00pages
e2000077issue
17-18eissn
1615-9853issn
1615-9861journal_volume
20pub_type
杂志文章,评审相关文献
PROTEOMICS文献大全abstract::Lumbar spinal stenosis (LSS) is a syndromic degenerative spinal disease and is characterized by spinal canal narrowing with subsequent neural compression causing gait disturbances. Although LSS is a major age-related musculoskeletal disease that causes large decreases in the daily living activities of the elderly, its...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201400442
更新日期:2015-05-01 00:00:00
abstract::FindPept (http://www.expasy.org/tools/findpept.html) is a software tool designed to identify the origin of peptide masses obtained by peptide mass fingerprinting which are not matched by existing protein identification tools. It identifies masses resulting from unspecific proteolytic cleavage, missed cleavage, proteas...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/1615-9861(200210)2:10<1435::AID-PROT1435>3
更新日期:2002-10-01 00:00:00
abstract::Increasing evidence suggests that root extracellular proteins are involved in interactions between roots and their soil environment. In the present study, exudates released by 6-day-old roots of the three legume species white lupin (Lupinus albus), soybean (Glycine max), and cowpea (Vigna sinensis) were collected unde...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201100629
更新日期:2012-11-01 00:00:00
abstract::Gram-negative bacteria release outer membrane vesicles (OMVs) as part of their normal growth that contain a range of cargo from their parent bacterium, including DNA, RNA, and proteins. The protein content of OMVs is suggested to be similar in composition to various sub-cellular locations of their parent bacterium. Ho...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201800209
更新日期:2019-01-01 00:00:00
abstract::In a comparative study, we investigated the influence of nine sample preparation workflows and seven different lysis buffers for qualitative and quantitative analysis of the human adipose tissue proteome. Adipose tissue is not just a fat depot but also an endocrine organ, which cross-talks with other tissue types and ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201500462
更新日期:2017-03-01 00:00:00
abstract::Studying microbial interactions by MALDI mass spectrometry imaging (MSI) directly from growing media is a difficult task if high sensitivity is demanded. We present a quick and robust sample preparation strategy for growing fungi (Trichoderma atroviride, Rhizoctonia solani) on glass slides to establish a miniaturized ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201500510
更新日期:2016-06-01 00:00:00
abstract::Low root temperature causes a decrease in water uptake, which leads to mineral and nutrient deficiencies with potentially decreased root and shoot growth. Differential temperature effects in plants have been studied extensively, however, the effect of root chilling on the global protein expression in shoots has not be...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201200475
更新日期:2013-06-01 00:00:00
abstract::Proteolytic modification of components of the extracellular milieu by metalloproteinases plays important roles in the regulation of multiple cellular and physiological processes and pathological conditions. ADAMTS1 is a secreted enzyme of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) fami...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200500446
更新日期:2006-04-01 00:00:00
abstract::Renal cell carcinoma (RCC) originates in the renal cortex. It accounts for 2-3 percent of all cancers occurring in adults and it is characterised by lack of early clinical manifestations, unpredictable outcome, and absence of effective treatment modalities except early surgery. RCC comprises a heterogeneous group of t...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/1615-9861(200110)1:10<1288::AID-PROT1288>3
更新日期:2001-10-01 00:00:00
abstract::Four rhoptry proteins (ROP) of Toxoplasma gondii previously identified with mAb have been affinity purified and analyzed by MS; the data obtained allowed the genomic sequences to be assigned to these proteins. As previously suggested for some of them by antibody crossreactivity, these proteins were shown to belong to ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200600187
更新日期:2006-11-01 00:00:00
abstract::Experimental identification of expressed proteins by proteomics constitutes the most reliable approach to identify genomic location and structure of protein-coding genes and substantially complements computational genome annotation. Channel catfish herpesvirus (CCV) is a simple comparative model for understanding herp...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200800397
更新日期:2009-05-01 00:00:00
abstract::Embryo implantation into maternal endometrium is critical for initiation and establishment of pregnancy, requiring developmental synchrony between endometrium and blastocyst. However, factors regulating human endometrial-embryo cross talk and facilitate implantation remain largely unknown. Extracellular vesicles (EVs)...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201800423
更新日期:2019-12-01 00:00:00
abstract::Various types of unwanted and uncontrollable signal variations in MS-based metabolomics and proteomics datasets severely disturb the accuracies of metabolite and protein profiling. Therefore, pooled quality control (QC) samples are often employed in quality management processes, which are indispensable to the success ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201900264
更新日期:2019-10-01 00:00:00
abstract::The proteins recovered in exhaled breath condensate (EBC) might be used to non-invasively monitor respiratory diseases. However, the range of proteins and their source are still unresolved and contamination by saliva or a similar protein pattern in the nasal and bronchial compartments may make interpretation of the da...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/1615-9861(200206)2:6<690::AID-PROT690>3.0.
更新日期:2002-06-01 00:00:00
abstract::A large repertoire of immunological methods permits monitoring the interaction of antibodies with their specific antigen. However, recognition of a protein by a conformation-specific antibody represents a challenge because native conditions must be kept throughout the assay. Native immunoblotting of blue native gels c...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200900468
更新日期:2010-01-01 00:00:00
abstract::Trichomonas vaginalis, a sexually transmitted parasite, has many cysteine proteinases (CPs); some are involved in trichomonal pathogenesis, express during infection, and antibodies against CPs have been detected in patient sera. The goal of this study was to identify the antigenic proteinases of T. vaginalis as potent...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200900479
更新日期:2010-02-01 00:00:00
abstract::High-affinity molecular pairs provide a convenient and flexible modular base for the design of molecular probes and protein/antigen assays. Specificity and sensitivity performance indicators of a bioassay critically depend on the dissociation constant (K(D)) of the molecular pair, with avidin:biotin being the state-of...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201200491
更新日期:2013-05-01 00:00:00
abstract::Human butyrophilin (BTN) expression in milk fat globule (MFGM) was evaluated using two dimensional electrophoresis (2-DE) as the separation technique, and peptide mass mapping by matrix-assisted laser desorption/ionisation-mass spectrometry (MALDI-MS) as the identification tool. Since milk composition changes througho...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/1615-9861(200207)2:7<850::AID-PROT850>3.0.
更新日期:2002-07-01 00:00:00
abstract::Antibiotics disturb the physiological homeostasis of bacterial cells by interfering with essential cellular functions or structures. The bacterial proteome adjusts quickly in response to antibiotic challenge. This physiological response is specifically tailored to overcome the inflicted damage and, thus, closely linke...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.201100046
更新日期:2011-08-01 00:00:00
abstract::In shotgun proteomics, proteins can be fractionated by 1-D gel electrophoresis and digested into peptides, followed by liquid chromatography to separate the peptide mixture. Mass spectrometry generates hundreds of thousands of tandem mass spectra from these fractions, and proteins are identified by database searching....
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200500318
更新日期:2006-02-01 00:00:00
abstract::Protein complexes carry out almost the entire signaling and functional processes in the cell. The protein complex complement of a cell, and its network of complex-complex interactions, is referred to here as the complexome. Computational methods to predict protein complexes from proteomics data, resulting in network r...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.201300230
更新日期:2014-01-01 00:00:00
abstract::Sequencing of the human genome opened the way to the exploration of the proteome and this has lead to the identification of large numbers of proteins in complex biological samples. The identification of diagnostic patterns in samples taken from patients to aid diagnosis is in the early stages of development. The solut...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.200700665
更新日期:2008-02-01 00:00:00
abstract::Two new iodoacetamide-substituted cyanines, C3NIASO3 and C5NIASO3, were synthesized starting from hemicyanine and were utilized for labeling plasma proteins. Specificity, sensitivity and feasibility for SH residues was tested utilizing an equimolar mixture of standard proteins and with normal plasma. Oxidized plasma p...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200800254
更新日期:2009-01-01 00:00:00
abstract::There is ample evidence for the involvement of protein phosphorylation on serine/threonine/tyrosine in bacterial signaling and regulation, but very few exact phosphorylation sites have been experimentally determined. Recently, gel-free high accuracy MS studies reported over 150 phosphorylation sites in two bacterial m...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200800285
更新日期:2009-01-01 00:00:00
abstract::In mass spectrometry-based proteomics, protein identification results usually consist of peptide sequences and database-dependent accession identifiers of the matching proteins. Often certain annotations are only available in particular databases that in turn must be queried by a certain identifier. In order to simpli...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200600018
更新日期:2006-08-01 00:00:00
abstract::In prokaryotes, acyl carrier protein (ACP) is a cofactor central to a myriad of syntheses, including fatty acid and phospholipid synthesis. To fulfill its function, ACP must therefore interact with a multitude of different enzymes, which includes the thioesterase YbgC. We found a specific interaction between ACP and Y...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200500115
更新日期:2006-01-01 00:00:00
abstract::The 2-D peptide separations employing mixed mode reversed phase anion exchange (MM (RP-AX)) HPLC in the first dimension in conjunction with RP chromatography in the second dimension were developed and utilised for shotgun proteome analysis. Compared with strong cation exchange (SCX) typically employed for shotgun prot...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200900669
更新日期:2010-08-01 00:00:00
abstract::Cyanobacteria are oxygenic photosynthetic prokaryotes and play a crucial role in the Earth's carbon and nitrogen cycles. The photoautotrophic cyanobacterium Anabaena sp. PCC 7120 has the ability to fix atmospheric nitrogen in heterocysts and produce hydrogen as a byproduct through a nitrogenase. In order to improve hy...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201800332
更新日期:2019-10-01 00:00:00
abstract::Reporter ion-based methods are among the major techniques to quantify peptides and proteins. Two main labels, tandem mass tag (TMT) and iTRAQ, are widely used by the proteomics community. They are, however, often applied as out-of-the-box methods, without thorough quality control. Thus, due to undiscovered limitations...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201000711
更新日期:2011-03-01 00:00:00
abstract::A brief period of ischemia followed by timely reperfusion may lead to prolonged, yet reversible, contractile dysfunction (myocardial stunning). Damage to the myocardium occurs not only during ischemia, but also during reperfusion, where a massive release of oxygen-free radicals (OFR) occurs. We have previously utilize...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200600219
更新日期:2006-12-01 00:00:00