Abstract:
:FindPept (http://www.expasy.org/tools/findpept.html) is a software tool designed to identify the origin of peptide masses obtained by peptide mass fingerprinting which are not matched by existing protein identification tools. It identifies masses resulting from unspecific proteolytic cleavage, missed cleavage, protease autolysis or keratin contaminants. It also takes into account post-translational modifications derived from the annotation of the SWISS-PROT database or supplied by the user, and chemical modifications of peptides. Based on a number of experimental examples, we show that the commonly held rules for the specificity of tryptic cleavage are an oversimplification, mainly because of effects of neighboring residues, experimental conditions, and contaminants present in the enzyme sample.
journal_name
Proteomicsjournal_title
Proteomicsauthors
Gattiker A,Bienvenut WV,Bairoch A,Gasteiger Edoi
10.1002/1615-9861(200210)2:10<1435::AID-PROT1435>3subject
Has Abstractpub_date
2002-10-01 00:00:00pages
1435-44issue
10eissn
1615-9853issn
1615-9861journal_volume
2pub_type
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