Unrestricted identification of modified proteins using MS/MS.

abstract：:Protein arginine methylation is a PTM catalyzed by an evolutionarily conserved family of enzymes called protein arginine methyltransferases (PRMTs), with PRMT1 being the most conserved member of this enzyme family. This modification has emerged to be an important regulator of protein functions. To better understand th...

journal_title：Proteomics

authors： Jackson CA,Yadav N,Min S,Li J,Milliman EJ,Qu J,Chen YC,Yu MC

更新日期：2012-11-01 00:00:00

abstract：:Since its discovery more than 50 years ago, post-translational modification (PTM) of proteins via methylation has grown in prominence, its involvement having been recognised in a number of central processes in the cell. Of these, the best characterised is its role in the epigenetic code. However, there is increasing e...

journal_title：Proteomics

authors： Erce MA,Pang CN,Hart-Smith G,Wilkins MR

更新日期：2012-02-01 00:00:00

abstract：:Most proteins in all organisms undergo crucial N-terminal modifications involving N-terminal methionine excision, N-alpha-acetylation or N-myristoylation (N-Myr), or S-palmitoylation. We investigated the occurrence of these poorly annotated but essential modifications in proteomes, focusing on eukaryotes. Experimental...

journal_title：Proteomics

authors： Martinez A,Traverso JA,Valot B,Ferro M,Espagne C,Ephritikhine G,Zivy M,Giglione C,Meinnel T

更新日期：2008-07-01 00:00:00

abstract：:Daphnia pulex is a keystone species for aquatic habitats and an ecological/evolution model organism. Although significant progress has been made on characterizing its genome, the D. pulex proteome remains largely uncharacterized partially due to abnormally high protein degradation during homogenization and emphasis on...

journal_title：Proteomics

authors： Steevensz A,Gombar R,Vergilino R,Cristescu ME,Vacratsis PO

更新日期：2018-08-01 00:00:00

abstract：:Proteolysis affects every protein at some point in its life cycle. Many biomarkers of disease or cancer are stable proteolytic fragments in biological fluids. There is great interest and a challenge in proteolytically modified protein study to identify physiologic protease-substrate relationships and find potential bi...

journal_title：Proteomics

authors： Shen C,Yu Y,Li H,Yan G,Liu M,Shen H,Yang P

更新日期：2012-06-01 00:00:00

abstract：:Ovarian cancer is one of the leading causes of mortality by gynecological cancer. Despite good response to surgery and initial chemotherapy, essentially based on cisplatin (cis-diamino-dichloro-platinum(II) (CDDP)) compounds, frequent recurrences with chemoresistance acquisition are responsible for poor prognosis. Sev...

journal_title：Proteomics

authors： Le Moguen K,Lincet H,Marcelo P,Lemoisson E,Heutte N,Duval M,Poulain L,Vinh J,Gauduchon P,Baudin B

更新日期：2007-11-01 00:00:00

abstract：:Cells have a rich inner structure that is commonly explored by microscopy. Classical biochemical methods that break apart the cells and fractionate them along a gradient have now gotten a new lease on life through modern methods of mass spectrometry-based proteomics. Their common principle is to comprehensively measur...

journal_title：Proteomics

authors： Mann M

更新日期：2020-08-03 00:00:00

abstract：:Plant cell walls (CWs) contain a large proportion of polysaccharides (90-95% of CW mass) and proteins (5-10%) that play major roles in CW plasticity during development and in response to environmental cues. Here, we present CW proteomics data of Arabidopsis thaliana roots. Plants were cultivated in hydroponic conditio...

journal_title：Proteomics

authors： Nguyen-Kim H,San Clemente H,Balliau T,Zivy M,Dunand C,Albenne C,Jamet E

更新日期：2016-02-01 00:00:00

abstract：:MS-based protein identification is an important part of both gel-based and gel-free proteome studies. The MASCOT search engine (http://www.matrixscience.com) provides one of the most popular automated algorithms for this task. Here we present an open-source software library written in Java that parses raw MASCOT resul...

journal_title：Proteomics

authors： Helsens K,Martens L,Vandekerckhove J,Gevaert K

更新日期：2007-02-01 00:00:00

abstract：:Puccinia triticina causes leaf rust, a disease that causes annual yield losses in wheat. It is an obligate parasite that invades the host leaf and forms intracellular structures called haustoria, which obtain nutrients and suppress host immunity using secreted proteins called effectors. Since effector proteins act at ...

journal_title：Proteomics

authors： Rampitsch C,Günel A,Beimcik E,Mauthe W

更新日期：2015-04-01 00:00:00

abstract：:The severe pulmonary form of leptospirosis (SPFL) is an especially serious and rapid disease process characterized by alveolar hemorrhage and acute respiratory failure. The outer membrane of Leptospira facilitates direct interactions with the environs and likely contains important constituents involved during infectio...

journal_title：Proteomics

authors： Nally JE,Whitelegge JP,Aguilera R,Pereira MM,Blanco DR,Lovett MA

更新日期：2005-01-01 00:00:00

abstract：:The spermatozoon is an accessible cell which can be easily purified and therefore it is particularly well suited for proteomic analysis. It is also an extremely differentiated cell with very marked genetic, cellular, functional and chromatin changes as compared to other cells, and has profound implications for fertili...

journal_title：Proteomics

authors： Oliva R,de Mateo S,Estanyol JM

更新日期：2009-02-01 00:00:00

abstract：:Radiation therapy (RT), combined with chemotherapy, is currently the standard adjuvant approach for UICC-stage II and III rectal cancer patients. Individual rectal tumors display wide ranges of radiosensitivity (RS). The aim of the present study was to identify proteins associated with radioresistance (RR), with the f...

journal_title：Proteomics

authors： Allal AS,Kähne T,Reverdin AK,Lippert H,Schlegel W,Reymond MA

更新日期：2004-08-01 00:00:00

abstract：:Mitochondria consist of four compartments, outer membrane, intermembrane space, inner membrane, and matrix; each harboring specific functions and structures. In this study, we used LC-MS/MS to characterize the protein composition of Trypanosoma brucei mitochondrial (mt) membranes, which were enriched by different bioc...

journal_title：Proteomics

authors： Acestor N,Panigrahi AK,Ogata Y,Anupama A,Stuart KD

更新日期：2009-12-01 00:00:00

abstract：:Lectin microarray is an emerging technique, which will accelerate glycan profiling and discovery of glycan-related biomarkers. One of the most important stages in realizing the potential of the technique is to achieve sufficiently high sensitivity to detect even the low concentrations of some target glycoproteins whic...

journal_title：Proteomics

authors： Uchiyama N,Kuno A,Tateno H,Kubo Y,Mizuno M,Noguchi M,Hirabayashi J

更新日期：2008-08-01 00:00:00

abstract：:This study aimed at establishing a sensitive multiple reaction monitoring-mass spectrometry (MRM-MS) method for the quantification of the drug metabolizing cytochrome P450 (CYP)3A4 enzyme in human liver homogenates. Liver samples were subjected to trypsin digestion. MRM-MS analyses were performed using three transitio...

journal_title：Proteomics

authors： Cieślak A,Kelly I,Trottier J,Verreault M,Wunsch E,Milkiewicz P,Poirier G,Droit A,Barbier O

更新日期：2016-11-01 00:00:00

abstract：:CREC proteins constitute a family of EF-hand calcium binding proteins localized to the secretory pathway. Calumenin is the only member known to be secreted. Recently, it was shown that thrombin-activated thrombocytes liberate calumenin, which also is found in atherosclerotic lesions but not in normal vasculature. To s...

journal_title：Proteomics

authors： Ostergaard M,Hansen GA,Vorum H,Honoré B

更新日期：2006-06-01 00:00:00

abstract：:Tyrosol (TYR, 2-(4-hydroxyphenyl)ethanol), one of the main phenols in olive oil and olive fruit, significantly strengthens resistance to thermal and oxidative stress in the nematode Caenorhabditis elegans and extends its lifespan. To elucidate the cellular functions regulated by TYR, we have used a proteomic procedure...

journal_title：Proteomics

authors： Cañuelo A,Peragón J

更新日期：2013-10-01 00:00:00

abstract：:The cancer tissue proteome has enormous potential as a source of novel predictive biomarkers in oncology. Progress in the development of mass spectrometry (MS)-based tissue proteomics now presents an opportunity to exploit this by applying the strategies of comprehensive molecular profiling and big-data analytics that...

journal_title：Proteomics

authors： Tully B,Balleine RL,Hains PG,Zhong Q,Reddel RR,Robinson PJ

更新日期：2019-11-01 00:00:00

abstract：:In the era of quantitative proteomics, where mass spectrometry plays a pivotal role, in particular associated with the use of data-independent acquisition, it is time to perform an overview of this growing field with special focus on one of the most promising approaches: SWATH-MS, and to present future perspectives fo...

journal_title：Proteomics

authors： Anjo SI,Santa C,Manadas B

更新日期：2017-02-01 00:00:00

abstract：:Currently, most researches on the properties of interacting proteins, hubs in particular, are mainly based on an aggregated network of binary interactions. Here, by projecting a yeast-aggregated cell cycle network into four phase (G1, S, G2 and M) sub-networks, we explored the properties of the hubs of the aggregated ...

journal_title：Proteomics

authors： Wu X,Guo J,Zhang DY,Lin K

更新日期：2009-10-01 00:00:00

abstract：:An improved method for peptide sequencing based on acetylation/deuteroacetylation in conjunction with ESI MS is introduced. Derivatization with a 1:1 mixture of acetic anhydride and deuterated acetic anhydride incorporates a stable isotope label into the analyzed molecule. This approach has been initially applied to F...

journal_title：Proteomics

authors： Noga MJ,Lewandowski JJ,Suder P,Silberring J

更新日期：2005-11-01 00:00:00

abstract：:The function of a protein is modulated by its abundance and its degree of specific post-translational modifications such as phosphorylation, glycosylation or truncation. Consequently, changes of protein concentration and the extent of their post-translational modifications has a great influence on the activity of intr...

journal_title：Proteomics

authors： Moritz B,Meyer HE

更新日期：2003-11-01 00:00:00

abstract：:During the peri-implantation period, molecular signaling between embryo and endometrium (layer of tissue lining the uterus lumen) is supposed to be crucial for the maintenance of pregnancy. To investigate embryo-induced alterations in the proteome of bovine endometrium in the preattachment period (day 18), we used mon...

journal_title：Proteomics

authors： Berendt FJ,Fröhlich T,Schmidt SE,Reichenbach HD,Wolf E,Arnold GJ

更新日期：2005-07-01 00:00:00

abstract：:Urinary microvesicles constitute a rich source of membrane-bound and intracellular proteins that may provide important clues of pathophysiological mechanisms in renal disease. In the current study, we analyzed and compared the proteome of urinary microvesicles from patients with idiopathic membranous nephropathy (iMN)...

journal_title：Proteomics

authors： Rood IM,Merchant ML,Wilkey DW,Zhang T,Zabrouskov V,van der Vlag J,Dijkman HB,Willemsen BK,Wetzels JF,Klein JB,Deegens JK

更新日期：2015-11-01 00:00:00

abstract：:Deciphering a complete landscape of protein-RNA interactions in the human proteome remains an elusive challenge. We computationally elucidate RNA binding proteins (RBPs) using an approach that complements previous efforts. We employ two modern complementary sequence-based methods that provide accurate predictions from...

journal_title：Proteomics

authors： Chowdhury S,Zhang J,Kurgan L

更新日期：2018-11-01 00:00:00

abstract：:The basic problem of complexity poses a significant challenge for proteomic studies. To date two-dimensional gel electrophoresis (2-DE) followed by enzymatic in-gel digestion of the peptides, and subsequent identification by mass spectrometry (MS) is the most commonly used method to analyze complex protein mixtures. H...

journal_title：Proteomics

authors： Ihling C,Sinz A

更新日期：2005-05-01 00:00:00

abstract：:Collagen-related peptide (CRP) stimulates powerful activation of platelets through the glycoprotein VI (GPVI)-FcR gamma-chain complex. We have combined proteomics and traditional biochemistry approaches to study the proteome of CRP-activated platelets, focusing in detail on tyrosine phosphorylation. In two separate ap...

journal_title：Proteomics

authors： García A,Senis YA,Antrobus R,Hughes CE,Dwek RA,Watson SP,Zitzmann N

更新日期：2006-10-01 00:00:00

abstract：:Oviductal fluid (ODF) proteins modulate and support reproductive processes in the oviduct. In the present study, proteins involved in the biological events that precede fertilization have been identified in the rabbit ODF proteome, isolated from the ampulla and isthmus of the oviduct at different time points within 8 ...

journal_title：Proteomics

authors： Yu H,Hackenbroch L,Meyer FRL,Reiser J,Razzazi-Fazeli E,Nöbauer K,Besenfelder U,Vogl C,Brem G,Mayrhofer C

更新日期：2019-03-01 00:00:00

abstract：:A multiplexed fluorescence immunoassay using a novel planar waveguide technology-based microarray system, ZeptoMARK (Zeptosens), was developed to detect simultaneously urokinase-type plasminogen activator (uPA), plasminogen activator inhibitor-1 (PAI-1), and vascular endothelial growth factor (VEGF) in extracts of bre...

journal_title：Proteomics

authors： Weissenstein U,Schneider MJ,Pawlak M,Cicenas J,Eppenberger-Castori S,Oroszlan P,Ehret S,Geurts-Moespot A,Sweep FC,Eppenberger U

更新日期：2006-03-01 00:00:00