Proteomics analysis in Caenorhabditis elegans to elucidate the response induced by tyrosol, an olive phenol that stimulates longevity and stress resistance.

abstract：:Acceleration of liver regeneration could be of great clinical benefit in various liver-associated diseases. However, at present little is known about therapeutic interventions to enhance this regenerative process. Our limited understanding and the complexity of the mechanisms involved have prevented the identification...

journal_title：Proteomics

authors： Strey CW,Winters MS,Markiewski MM,Lambris JD

更新日期：2005-01-01 00:00:00

abstract：:Sepsis is an extreme host response to infection that leads to loss of organ function and cardiovascular integrity. Mortality from sepsis is on the rise. Despite more than three decades of research and clinical trials, specific diagnostic and therapeutic strategies for sepsis are still absent. The use of LFQ- and TMT-b...

journal_title：Proteomics

authors： Pimienta G,Heithoff DM,Rosa-Campos A,Tran M,Esko JD,Mahan MJ,Marth JD,Smith JW

更新日期：2019-03-01 00:00:00

abstract：:Proteolytic digestion is an essential step in proteomic sample processing. While this step has traditionally been implemented in homogeneous (solution) format, there is a growing trend to use heterogeneous systems in which the enzyme is immobilized on hydrogels or other solid supports. Here, we introduce the use of im...

journal_title：Proteomics

authors： Luk VN,Fiddes LK,Luk VM,Kumacheva E,Wheeler AR

更新日期：2012-05-01 00:00:00

abstract：:CID has become a routine method for fragmentation of peptides in shotgun proteomics, whereas electron transfer dissociation (ETD) has been described as a preferred method for peptides carrying labile PTMs. Though both of these fragmentation techniques have their obvious advantages, they also have their own drawbacks. ...

journal_title：Proteomics

authors： Kim MS,Zhong J,Kandasamy K,Delanghe B,Pandey A

更新日期：2011-06-01 00:00:00

abstract：:Mycobacterium avium subsp. paratuberculosis has long been recognized as the causative agent of Johne's disease, a chronic inflammatory intestinal disease of sheep, cattle and other ruminants. Mycobacterial cells are extremely hardy, and proteomic analyses require the use of harsh conditions to effect their disruption....

journal_title：Proteomics

authors： Lanigan MD,Vaughan JA,Shiell BJ,Beddome GJ,Michalski WP

更新日期：2004-04-01 00:00:00

abstract：:We present here an effective technique for the large-scale separation and identification of N-linked glycoproteins from Chang liver cells, the human normal liver cells. To enrich N-linked glycoproteins from the whole cells, a procedure containing the lysis of human liver cells, the solubilization of total proteins, le...

journal_title：Proteomics

authors： Xu Z,Zhou X,Lu H,Wu N,Zhao H,Zhang L,Zhang W,Liang YL,Wang L,Liu Y,Yang P,Zha X

更新日期：2007-07-01 00:00:00

abstract：:Since detergent-resistant lipid rafts play important roles in the signal transduction for myogenesis, their comprehensive proteomic analysis could provide new insights to understand their function in myotubes. Here, the detergent-resistant lipid rafts were isolated from C2C12 myotubes and analyzed by capillary RPLC/MS...

journal_title：Proteomics

authors： Kim BW,Lee JW,Choo HJ,Lee CS,Jung SY,Yi JS,Ham YM,Lee JH,Hong J,Kang MJ,Chi SG,Hyung SW,Lee SW,Kim HM,Cho BR,Min DS,Yoon G,Ko YG

更新日期：2010-07-01 00:00:00

abstract：:O-GlcNAcylation is a dynamic PTM of nuclear and cytoplasmic proteins, regulated by O-GlcNAc transferase (OGT) and O-GlcNAcase, which catalyze the addition and removal of O-GlcNAc, respectively. This modification is associated with glucose metabolism, which plays important roles in many diseases including cancer. Altho...

journal_title：Proteomics

authors： Champattanachai V,Netsirisawan P,Chaiyawat P,Phueaouan T,Charoenwattanasatien R,Chokchaichamnankit D,Punyarit P,Srisomsap C,Svasti J

更新日期：2013-07-01 00:00:00

abstract：:Gram-negative bacteria release outer membrane vesicles (OMVs) as part of their normal growth that contain a range of cargo from their parent bacterium, including DNA, RNA, and proteins. The protein content of OMVs is suggested to be similar in composition to various sub-cellular locations of their parent bacterium. Ho...

journal_title：Proteomics

authors： Zavan L,Bitto NJ,Johnston EL,Greening DW,Kaparakis-Liaskos M

更新日期：2019-01-01 00:00:00

abstract：:Glycosylphosphatidylinositol-anchored proteins (GPI-APs) are an important class of glycoproteins that are tethered to the surface of mammalian cells via the lipid GPI. GPI-APs have been implicated in many important cellular functions including cell adhesion, cell signaling, and immune regulation. Proteomic identificat...

journal_title：Proteomics

authors： Cortes LK,Vainauskas S,Dai N,McClung CM,Shah M,Benner JS,Corrêa IR Jr,VerBerkmoes NC,Taron CH

更新日期：2014-11-01 00:00:00

abstract：:High hydrostatic pressure (HHP) exerts diverse effects on microorganisms, leading to stress response and cell death. While inactivation of microorganisms by lethal HHP is well investigated in the context of food preservation and the hygienic safety of minimal food processes, sublethal HHP stress response and its effec...

journal_title：Proteomics

authors： Hörmann S,Scheyhing C,Behr J,Pavlovic M,Ehrmann M,Vogel RF

更新日期：2006-03-01 00:00:00

abstract：:Identification of differential proteomic responses to cadmium (Cd) would provide a means for better understanding the survival mechanisms of the earthworm, Eisenia fetida, living in a Cd-polluted environment, and the stress responses can be assessed by ecotoxiptoromics approaches. Extracts of whole earthworm collected...

journal_title：Proteomics

authors： Wang X,Chang L,Sun Z,Zhang Y,Yao L

更新日期：2010-12-01 00:00:00

abstract：:Sandwich ELISA microarrays have great potential for validating disease biomarkers. Each ELISA relies on robust-affinity reagents that retain activity when immobilized on a solid surface or when labeled for detection. Single-chain antibodies (scFv) are affinity reagents that have greater potential for high-throughput p...

journal_title：Proteomics

authors： Seurynck-Servoss SL,Baird CL,Miller KD,Pefaur NB,Gonzalez RM,Apiyo DO,Engelmann HE,Srivastava S,Kagan J,Rodland KD,Zangar RC

更新日期：2008-06-01 00:00:00

abstract：:In the present study, changes in protein synthesis patterns after salt shock visualized by 35S-methionine labeling and the changed protein composition in salt-acclimated cells of the cyanobacterium Synechocystis sp. strain PCC 6803 were analyzed by a combination of 2-DE for protein separation and PMF for protein ident...

journal_title：Proteomics

authors： Fulda S,Mikkat S,Huang F,Huckauf J,Marin K,Norling B,Hagemann M

更新日期：2006-05-01 00:00:00

abstract：:Metabolite flux to UDP-GlcNAc and Golgi N-glycan biosynthesis regulates surface residency of glycoprotein receptors and transporters, and thus sensitivities of cells to extracellular cues. Salvage of GlcNAc increases UDP-GlcNAc and branching of N-glycans progressively, but displays an optimum for cell proliferation an...

journal_title：Proteomics

authors： Lau KS,Khan S,Dennis JW

更新日期：2008-08-01 00:00:00

abstract：:We have combined several key sample preparation steps for the use of a liquid matrix system to provide high analytical sensitivity in automated ultraviolet -- matrix-assisted laser desorption/ionisation -- mass spectrometry (UV-MALDI-MS). This new sample preparation protocol employs a matrix-mixture which is based on ...

journal_title：Proteomics

authors： Cramer R,Corless S

更新日期：2005-02-01 00:00:00

abstract：:Autosomal dominant tubulointerstitial kidney disease associated to the MUC1 gene (ADTKD-MUC1; formerly MCKD1) belongs to a heterogeneous group of rare hereditary kidney diseases that is prototypically caused by frameshift mutations in the MUC1 repeat domain. The mutant MUC1 (insC) lacks the transmembrane domaine, exhi...

journal_title：Proteomics

authors： Staubach S,Wenzel A,Beck BB,Rinschen MM,Müller S,Hanisch FG

更新日期：2018-04-01 00:00:00

abstract：:The CpG motif of bacterial DNA (CpG-DNA) is a potent immunostimulating agent whose mechanism of action is not yet clear. Here, we used both DNA microarray and proteomic approaches to investigate the effects of oligodeoxynucleotides containing the CpG motif (CpG-ODN) on gene transcription and protein expression profile...

journal_title：Proteomics

authors： Kuo CC,Kuo CW,Liang CM,Liang SM

更新日期：2005-03-01 00:00:00

abstract：:Abnormal phosphorylation contributes to the formation of neurofibrillary tangles in Alzheimer's disease (AD), but may play other signaling roles during AD pathogenesis. In this study, we employed IMAC followed by LC-MS/MS to identify phosphopeptides from eight individual AD and eight age-matched control postmortem hum...

journal_title：Proteomics

authors： Dammer EB,Lee AK,Duong DM,Gearing M,Lah JJ,Levey AI,Seyfried NT

更新日期：2015-01-01 00:00:00

abstract：:Oligomeric ubiquitin structures (i.e. ubiquitin "chains") may be formed through any of seven different lysine residues in the polypeptide, or via the amine group of Met 1. Different types of ubiquitin chains can confer very different biological outcomes to a protein substrate, yet the structural characteristics of E2s...

journal_title：Proteomics

authors： Hong JH,Ng D,Srikumar T,Raught B

更新日期：2015-09-01 00:00:00

abstract：:Human saliva contains a large number of proteins and peptides (salivary proteome) that help maintain homeostasis in the oral cavity. Global analysis of human salivary proteome is important for understanding oral health and disease pathogenesis. In this study, large-scale identification of salivary proteins was demonst...

journal_title：Proteomics

authors： Hu S,Xie Y,Ramachandran P,Ogorzalek Loo RR,Li Y,Loo JA,Wong DT

更新日期：2005-04-01 00:00:00

abstract：:RADARS, a rapid, automated, data archiving and retrieval software system for high-throughput proteomic mass spectral data processing and storage, is described. The majority of mass spectrometer data files are compatible with RADARS, for consistent processing. The system automatically takes unprocessed data files, iden...

journal_title：Proteomics

authors： Field HI,Fenyö D,Beavis RC

更新日期：2002-01-01 00:00:00

abstract：:In this work, we first immobilized tin(IV) ion on polydopamine-coated magnetic graphene (magG@PDA) to synthesize Sn4+ -immobilized magG@PDA (magG@PDA-Sn4+ ) and successfully applied the material to highly selective enrichment of phosphopeptides. The material gathered the advantages of large surface area of graphene, s...

journal_title：Proteomics

authors： Lin H,Deng C

更新日期：2016-11-01 00:00:00

abstract：:Protein ubiquitination is a powerful modulator of cellular functions. Classically linked to the degradation of proteins, it also plays a role in intracellular localization, DNA damage response, vesicle fusion events, and the immune and transcriptional responses. Ubiquitin is versatile and can code for several distinct...

journal_title：Proteomics

authors： Beaudette P,Popp O,Dittmar G

更新日期：2016-01-01 00:00:00

abstract：:High-affinity molecular pairs provide a convenient and flexible modular base for the design of molecular probes and protein/antigen assays. Specificity and sensitivity performance indicators of a bioassay critically depend on the dissociation constant (K(D)) of the molecular pair, with avidin:biotin being the state-of...

journal_title：Proteomics

authors： Sreenivasan VK,Kelf TA,Grebenik EA,Stremovskiy OA,Say JM,Rabeau JR,Zvyagin AV,Deyev SM

更新日期：2013-05-01 00:00:00

abstract：:Proteins extracted from leaf blades of rice plants infected with blast fungus, Magnaporthe grisea, were separated by two-dimensional polyacrylamide gel electrophoresis. The separated proteins were electroblotted onto a polyvinylidene difluoride membrane, and 63 proteins were analyzed by a gas-phase protein sequencer. ...

journal_title：Proteomics

authors： Konishi H,Ishiguro K,Komatsu S

更新日期：2001-09-01 00:00:00

abstract：:Interleukin (IL)-6 is a circulatory, pleiotropic cytokine with multiple roles in the immune system. Both IL-6 and the IL6 -174G>C promoter polymorphism have been linked to various diseases associated with inflammation. However, the mechanism by which the polymorphism influences disease risk is unclear. We postulated t...

journal_title：Proteomics

authors： Hegedus CM,Skibola CF,Bracci P,Holly EA,Smith MT

更新日期：2007-02-01 00:00:00

abstract：:Serum depletion strategies are commonly implemented in order to remove abundant proteins, increasing the number of proteins detected in a biomarker study. The IgY spin columns used in this study bind 12 and 14 primate proteins, respectively. 1-D SDS-PAGE and 2-DE revealed a suboptimal performance of the IgY spin colum...

journal_title：Proteomics

authors： Corrigan L,Jefferies C,Clive Lee T,Daly J

更新日期：2011-08-01 00:00:00

abstract：:The endothelium is a single layer of cells lining the inside face of all blood vessels. It constitutes a major metabolic organ which is critically involved in the generation and the regulation of multiple physiological and pathological processes such as coagulation, hemostasis, inflammation, atherosclerosis, angiogene...

journal_title：Proteomics

authors： Bruneel A,Labas V,Mailloux A,Sharma S,Vinh J,Vaubourdolle M,Baudin B

更新日期：2003-05-01 00:00:00

abstract：:By mining publicly accessible databases, we have developed a collection of tissue-specific predictive protein expression maps as a function of cancer histological state. Data analysis is applied to the differential expression of gene products in pooled libraries from the normal to the altered state(s). We wish to repo...

journal_title：Proteomics

authors： Medjahed D,Luke BT,Tontesh TS,Smythers GW,Munroe DJ,Lemkin PF

更新日期：2003-08-01 00:00:00