Abstract:
:Acceleration of liver regeneration could be of great clinical benefit in various liver-associated diseases. However, at present little is known about therapeutic interventions to enhance this regenerative process. Our limited understanding and the complexity of the mechanisms involved have prevented the identification of new targets for treatment. Here we propose a broad-range proteomic approach to this problem that makes possible the simultaneous study of different signaling and metabolic pathways on the liver proteome. Changes in protein expression in mouse livers (n = 5 per group) at 6 h and 12 h after partial hepatectomy and sham operation, as compared to untreated controls, were analyzed using two-dimensional gel electrophoresis, mass spectrometry (MS), and mass fingerprinting. Twelve proteins, identified by MS, were up-regulated by at least 2-fold after partial hepatectomy. These included adipose differentiation-related protein, gamma-actin, enoyl coenzyme A hydratase 1, serum amyloid A and eukaryotic translation initiation factor 3. These results indicate that liver regeneration following partial hepatectomy affects various signaling and metabolic pathways.
journal_name
Proteomicsjournal_title
Proteomicsauthors
Strey CW,Winters MS,Markiewski MM,Lambris JDdoi
10.1002/pmic.200400913subject
Has Abstractpub_date
2005-01-01 00:00:00pages
318-25issue
1eissn
1615-9853issn
1615-9861journal_volume
5pub_type
杂志文章相关文献
PROTEOMICS文献大全abstract::The aim of the study was to evaluate the SELDI-TOF MS technique for pancreatic islet research. Mouse islets were cultured at low or high glucose levels in the absence or presence of oleate and characterized by measuring insulin secretion and oxygen tension. Subsequently, the islets were protein profiled. Up to 200 dif...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200601019
更新日期:2007-09-01 00:00:00
abstract::Surface-enhanced laser desorption/ionization (SELDI) time of flight (TOF) is a mass spectrometry technology for measuring the composition of a sampled protein mixture. A mass spectrum contains peaks corresponding to proteins in the sample. The peak areas are proportional to the measured concentrations of the correspon...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200600181
更新日期:2006-10-01 00:00:00
abstract::Legume symbiosis with rhizobia results in the formation of a specialized organ, the root nodule, where atmospheric dinitrogen is reduced to ammonia. In Lotus japonicus (Lotus), several genes involved in nodule development or nodule function have been defined using biochemistry, genetic approaches, and high-throughput ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201300353
更新日期:2014-02-01 00:00:00
abstract::Ischemia/reperfusion (I/R) injury is a major consequence of a cardiovascular intervention. The study of changes of the left and right ventricle proteomes from hearts subjected to I/R may be a key to revealing the pathological mechanisms underlying I/R-induced heart contractile dysfunction. Isolated rat hearts were per...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201100604
更新日期:2012-08-01 00:00:00
abstract::Shewanella inhabit a wide variety of niches in nature and can utilize a broad spectrum of electron acceptors under anaerobic conditions. How they modulate their gene expression to adapt is poorly understood. ArcA, homologue of a global regulator controlling hundreds of genes involved in aerobic and anaerobic respirati...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201100651
更新日期:2012-06-01 00:00:00
abstract::Imbalance in protein homeostasis in specific subcellular organelles is alleviated through organelle-specific stress response pathways. As a canonical example of stress activated pathway, accumulation of misfolded proteins in ER activates unfolded protein response (UPR) in almost all eukaryotic organisms. However, very...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201300449
更新日期:2014-08-01 00:00:00
abstract::Uni- or multidimensional microcapillary liquid chromatography (microLC) matrix-assisted laser desorption/ionization (MALDI) tandem mass spectrometry (MS/MS) approaches have gained significant attention for quantifying and identifying proteins in complex biological samples. The off-line coupling of microLC with MS quan...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200300566
更新日期:2004-02-01 00:00:00
abstract::In this work, we first immobilized tin(IV) ion on polydopamine-coated magnetic graphene (magG@PDA) to synthesize Sn4+ -immobilized magG@PDA (magG@PDA-Sn4+ ) and successfully applied the material to highly selective enrichment of phosphopeptides. The material gathered the advantages of large surface area of graphene, s...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201600187
更新日期:2016-11-01 00:00:00
abstract::The main goal of this study was to analyze, using proteomic techniques, changes in protein expression of acute myeloid leukemia (AML) cells that could give insights into a better early prognosis for tumor pathophysiology. Proteomic analysis of different subtypes of AML cells was carried out using 2-DE and MALDI-TOF PM...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200500384
更新日期:2006-04-01 00:00:00
abstract::The Japanese red and pink corals are known to be precious because of their commercial value resulting from their use in ornaments, jewelry, and medicine. Precious corals are very interesting models for biomineralization studies and possess two different skeletal structures: an axial skeleton and an endoskeleton (scler...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201300519
更新日期:2014-11-01 00:00:00
abstract::Mycobacterium avium subsp. paratuberculosis has long been recognized as the causative agent of Johne's disease, a chronic inflammatory intestinal disease of sheep, cattle and other ruminants. Mycobacterial cells are extremely hardy, and proteomic analyses require the use of harsh conditions to effect their disruption....
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200300672
更新日期:2004-04-01 00:00:00
abstract::In an effort to identify tumor-associated proteins from plasma of tumor-bearing mice that may be used as diagnostic biomarkers, we developed a strategy that combines a tumor xenotransplantation model in nude mice with comparative proteomic technology. Five human cancer cell lines (SC-M1, HONE-1, CC-M1, OECM1, GBM 8401...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200400785
更新日期:2004-09-01 00:00:00
abstract::Targeted mass spectrometry-based proteomics approaches enable the simultaneous and reproducible quantification of multiple protein analytes across numerous conditions in biology and clinical studies. These approaches involve e.g. selected reaction monitoring (SRM) typically conducted on a triple quadrupole mass spectr...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201600323
更新日期:2017-03-01 00:00:00
abstract::In recent years, a new paradigm for genome annotation has emerged, termed "proteogenomics," that leverages peptide MS to annotate a genome. This is achieved by mapping peptides to a six-frame translation of a genome, including available splice databases, which may suggest refinements to gene models. Using this approac...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201400185
更新日期:2014-12-01 00:00:00
abstract::Tectona grandis L. (teak) is one of the premier hardwood timbers in the world, ranking at present in the top five tropical hardwood species in terms of worldwide plantation area. Characterization of the proteins present in teak leaves will provide a basis for the development of new tools aimed at assisting tree select...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201100183
更新日期:2012-04-01 00:00:00
abstract::Many types of cells transit in vitro from a two- to a three-dimensional growth, when they are exposed to microgravity. The underlying mechanisms are not yet understood. Hence, we investigated the impact of microgravity on protein content and growth behavior. For this purpose, the human thyroid cancer cells FTC-133 wer...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201500067
更新日期:2015-09-01 00:00:00
abstract::Protein arginine methyltransferase 5 (PRMT5) is a major enzyme responsible for generating monomethyl and symmetric dimethyl arginine in proteins. PRMT5 is essential for cell viability and development, and its overexpression is observed in a variety of cancers. In the present study, it is found that levels of PRMT5 pro...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201900367
更新日期:2020-05-28 00:00:00
abstract::Absolute protein concentration determination is becoming increasingly important in a number of fields including diagnostics, biomarker discovery and systems biology modeling. The recently introduced quantification concatamer methodology provides a novel approach to performing such determinations, and it has been appli...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201000454
更新日期:2011-01-01 00:00:00
abstract::MS protein identification and quantitation are key proteomic techniques in biological research. Besides identification of proteins, MS is used increasingly to characterize secondary protein modifications. This often requires trimming the analytical strategy to a specific type of modification. Direct analysis of protei...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200501332
更新日期:2006-01-01 00:00:00
abstract::The explosion of site- and context-specific in vivo phosphorylation events presents a potentially rich source of biological knowledge and calls for novel data analysis and modeling paradigms. Perhaps the most immediate challenge is delineating detected phosphorylation sites to their effector kinases. This is important...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.200900266
更新日期:2009-12-01 00:00:00
abstract::Protein arginine methylation is a PTM catalyzed by an evolutionarily conserved family of enzymes called protein arginine methyltransferases (PRMTs), with PRMT1 being the most conserved member of this enzyme family. This modification has emerged to be an important regulator of protein functions. To better understand th...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201200132
更新日期:2012-11-01 00:00:00
abstract::Ovarian cancer is one of the leading causes of mortality by gynecological cancer. Despite good response to surgery and initial chemotherapy, essentially based on cisplatin (cis-diamino-dichloro-platinum(II) (CDDP)) compounds, frequent recurrences with chemoresistance acquisition are responsible for poor prognosis. Sev...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200700231
更新日期:2007-11-01 00:00:00
abstract::Mulberry dwarf (MD) is a serious infectious disease of mulberry caused by phytoplasma. Infection with MD phytoplasma results in stress phenotypes of yellowing, phyllody, stunting, proliferation, and witches' broom. Physiological and biochemical analysis has shown that infection with MD phytoplasma causes an increase i...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200900012
更新日期:2009-12-01 00:00:00
abstract::Two Chinese bread wheat cultivars, Jinghua 9 and Zhongmai 175, distinct in grain weight and dough quality, were used to study proteome changes in the embryo and endosperm during grain development using a two-dimensional difference gel electrophoresis (2D-DIGE)-based proteomics approach. In total, 138 and 127 different...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201500371
更新日期:2016-05-01 00:00:00
abstract::To better understand the underlying molecular basis of leaf development in maize, a reference map of nuclear proteins in basal region of seedling leaf was established using a combination of 2DE and MALDI-TOF-MS. In total, 441 reproducible protein spots in nuclear proteome of maize leaf basal region were detected with ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201300147
更新日期:2014-05-01 00:00:00
abstract::Mitochondria consist of four compartments, outer membrane, intermembrane space, inner membrane, and matrix; each harboring specific functions and structures. In this study, we used LC-MS/MS to characterize the protein composition of Trypanosoma brucei mitochondrial (mt) membranes, which were enriched by different bioc...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200900354
更新日期:2009-12-01 00:00:00
abstract::Using proteomics in tandem with bioinformatics, the secretomes of nonaggressive and aggressive thyroid carcinoma (TC) cell lines were analyzed to detect potential biomarkers for tumor aggressiveness. A panel of nine proteins, activated leukocyte cell adhesion molecule (ALCAM/CD166), tyrosine-protein kinase receptor (A...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201200356
更新日期:2013-03-01 00:00:00
abstract::Proteins undergo PTM, which modulates their structure and regulates their function. Estimates of the PTM occurrence vary but it is safe to assume that there is an important gap between what is currently known and what remains to be discovered. The highest throughput and most comprehensive efforts to catalogue protein ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200900502
更新日期:2010-02-01 00:00:00
abstract::Glycosylation is a widespread PTM of proteins; the carbohydrate moieties provide various functional, immunological and structural aspects of both eukaryotic and prokaryotic glycoproteins. Traditional strategies used to analyse glycoprotein O-glycans involve glycoprotein isolation, followed by glycan release using solu...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200500331
更新日期:2006-05-01 00:00:00
abstract::High-affinity molecular pairs provide a convenient and flexible modular base for the design of molecular probes and protein/antigen assays. Specificity and sensitivity performance indicators of a bioassay critically depend on the dissociation constant (K(D)) of the molecular pair, with avidin:biotin being the state-of...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201200491
更新日期:2013-05-01 00:00:00