Quantitative phosphoproteomics of Alzheimer's disease reveals cross-talk between kinases and small heat shock proteins.

abstract：:Oviductal fluid (ODF) proteins modulate and support reproductive processes in the oviduct. In the present study, proteins involved in the biological events that precede fertilization have been identified in the rabbit ODF proteome, isolated from the ampulla and isthmus of the oviduct at different time points within 8 ...

journal_title：Proteomics

authors： Yu H,Hackenbroch L,Meyer FRL,Reiser J,Razzazi-Fazeli E,Nöbauer K,Besenfelder U,Vogl C,Brem G,Mayrhofer C

更新日期：2019-03-01 00:00:00

abstract：:Roots, leaves, and intermediate sections between roots and leaves (ISRL) of wheat seedlings show different physiological functions at the protein level. We performed the first integrative proteomic analysis of different tissues of the drought-tolerant wheat cultivar Hanxuan 10 (HX-10) and drought-sensitive cultivar Ch...

journal_title：Proteomics

authors： Hao P,Zhu J,Gu A,Lv D,Ge P,Chen G,Li X,Yan Y

更新日期：2015-05-01 00:00:00

abstract：:To develop a standard method for separating highly basic proteins in mammalian cells, we established a 2-D LC separation system coupled with chromatofocusing/nonporous RP column chromatography (CF/NPRPC) in a ProteomeLab PF2D system. After standardizing conditions for 2-D LC, a 2-D liquid protein map of uninfected mac...

journal_title：Proteomics

authors： Shin YK,Lee HJ,Lee JS,Paik YK

更新日期：2006-02-01 00:00:00

abstract：:Several lines of evidence suggest that hnRNPs A/B (hnRNPs A1, A2/B1, and A3) play an important role in proliferation, although the functional overlap among members of hnRNPs A/B remains largely unknown. In this study, we have employed RNAi knockdown and proteomic approaches to investigate the biological functions of h...

journal_title：Proteomics

authors： Yeh YM,Chen CY,Huang PR,Hsu CW,Wu CC,Wang TC

更新日期：2014-06-01 00:00:00

abstract：:Most proteins execute their functions through interacting with other proteins. Thus, understanding protein-protein interactions (PPIs) is essential to decipher biological functions in a living cell. To predict large-scale PPIs, effective and efficient computational approaches are desirable to integrate heterogeneous d...

journal_title：Proteomics

authors： Lin X,Chen XW

更新日期：2013-01-01 00:00:00

abstract：:Systematic detection of low-abundance proteins in human blood that may be putative disease biomarkers is complicated by an extremely wide range of protein abundances. Hence, depletion of major proteins is one potential strategy for enhancing detection sensitivity in serum or plasma. This study compared a recently comm...

journal_title：Proteomics

authors： Echan LA,Tang HY,Ali-Khan N,Lee K,Speicher DW

更新日期：2005-08-01 00:00:00

abstract：:MS protein identification and quantitation are key proteomic techniques in biological research. Besides identification of proteins, MS is used increasingly to characterize secondary protein modifications. This often requires trimming the analytical strategy to a specific type of modification. Direct analysis of protei...

journal_title：Proteomics

authors： Niggeweg R,Köcher T,Gentzel M,Buscaino A,Taipale M,Akhtar A,Wilm M

更新日期：2006-01-01 00:00:00

abstract：:The last 30 years Enterococcus faecium has become an important nosocomial pathogen in hospitals worldwide. The aim of this study was to obtain insight in the cell surface proteome of E. faecium when grown in laboratory and clinically relevant conditions. Enterococcus faecium E1162, a clinical blood stream isolate, was...

journal_title：Proteomics

authors： Sinnige JC,de Been M,Zhou M,Bonten MJ,Willems RJ,Top J

更新日期：2015-11-01 00:00:00

abstract：:Many types of cells transit in vitro from a two- to a three-dimensional growth, when they are exposed to microgravity. The underlying mechanisms are not yet understood. Hence, we investigated the impact of microgravity on protein content and growth behavior. For this purpose, the human thyroid cancer cells FTC-133 wer...

journal_title：Proteomics

authors： Riwaldt S,Pietsch J,Sickmann A,Bauer J,Braun M,Segerer J,Schwarzwälder A,Aleshcheva G,Corydon TJ,Infanger M,Grimm D

更新日期：2015-09-01 00:00:00

abstract：:The useful structural features of class II MHC molecules are rarely integrated into T-cell epitope predictions. We propose an approach that applies a novel expectation-maximization algorithm to align the naturally processed peptides selected by the class II MHC I-A(g7) molecule - focusing on the five MHC-specific anch...

journal_title：Proteomics

authors： Chang KY,Suri A,Unanue ER

更新日期：2007-02-01 00:00:00

abstract：:Cells are a fundamental unit of life, and the ability to study the phenotypes and behaviors of individual cells is crucial to understanding the workings of complex biological systems. Cell phenotypes (epigenomic, transcriptomic, proteomic, and metabolomic) exhibit dramatic heterogeneity between and within the differen...

journal_title：Proteomics

authors： Mincarelli L,Lister A,Lipscombe J,Macaulay IC

更新日期：2018-09-01 00:00:00

abstract：:Attribution of the most probable functions to proteins identified by proteomics is a significant challenge that requires extensive literature analysis. We have developed a system for automated prediction of implicit and explicit biologically meaningful functions for a proteomics study of the nucleolus. This approach u...

journal_title：Proteomics

authors： Schuemie M,Chichester C,Lisacek F,Coute Y,Roes PJ,Sanchez JC,Kors J,Mons B

更新日期：2007-03-01 00:00:00

abstract：:The current study used three different proteomic strategies, which differed by their extent of intact protein separation, to examine the proteome of a pluripotent mouse embryonic stem cell line, R1. Proteins from whole-cell lysates were subjected either to 2-D-LC, or 1-DE, or were unfractionated prior to enzymatic dig...

journal_title：Proteomics

authors： Gundry RL,Tchernyshyov I,Sheng S,Tarasova Y,Raginski K,Boheler KR,Van Eyk JE

更新日期：2010-07-01 00:00:00

abstract：:Accumulating evidence suggests that extracellular alpha-synuclein (eSNCA) plays an important role in the pathogenesis of Parkinson's disease or related synucleinopathies by inducing neurotoxicity directly or indirectly via microglial or astroglial activation. However, the mechanisms by which this occurs remain to be c...

journal_title：Proteomics

authors： Liu J,Shi M,Hong Z,Zhang J,Bradner J,Quinn T,Beyer RP,Mcgeer PL,Chen S,Zhang J

更新日期：2010-06-01 00:00:00

abstract：:The Asia Oceania Human Proteome Organisation (AOHUPO) has embarked on a Membrane Proteomics Initiative with goals of systematic comparison of strategies for analysis of membrane proteomes and discovery of membrane proteins. This multilaboratory project is based on the analysis of a subcellular fraction from mouse live...

journal_title：Proteomics

authors： Peng L,Kapp EA,Fenyö D,Kwon MS,Jiang P,Wu S,Jiang Y,Aguilar MI,Ahmed N,Baker MS,Cai Z,Chen YJ,Van Chi P,Chung MC,He F,Len AC,Liao PC,Nakamura K,Ngai SM,Paik YK,Pan TL,Poon TC,Salekdeh GH,Simpson RJ,Sirdesh

更新日期：2010-11-01 00:00:00

abstract：:The existence of a gamma-glutamyl cycle consisting of intracellular GSH synthesis, extrusion to the apoplastic space and recovery by gamma-glutamyl transferase (GGT)-assisted degradation into its constituent amino acids, has been demonstrated in plants. To address the significance of this cycle in plant cells, we perf...

journal_title：Proteomics

authors： Tolin S,Arrigoni G,Trentin AR,Veljovic-Jovanovic S,Pivato M,Zechman B,Masi A

更新日期：2013-06-01 00:00:00

abstract：:Modification through beta-elimination has proven to be a reliable first step in the approach for enrichment of serine/threonine-phopshorylated (Ser-/Thr) peptides. However, under harsh basic conditions, Ser-/Thr-glycosylated peptides are susceptible to beta-elimination as well. Therefore, we have optimized these condi...

journal_title：Proteomics

authors： Poot AJ,Ruijter E,Nuijens T,Dirksen EH,Heck AJ,Slijper M,Rijkers DT,Liskamp RM

更新日期：2006-12-01 00:00:00

abstract：:Separation or prefractionation of proteins by gel electrophoresis, followed by in-gel proteolytic digest and analysis by MS is a typical standard application in proteomics. For many laboratories, it is not cost-effective to use a robot with all its drawbacks. On the other hand, manual digest is time consuming and not ...

journal_title：Proteomics

authors： Franz T,Li X

更新日期：2012-08-01 00:00:00

abstract：:An improved method for sample preparation for MALDI-MS and MS/MS using AnchorChip targets is presented. The method, termed the SMW method (sample, matrix wash), results in better sensitivity for peptide mass fingerprinting as well as for sequencing by MS/MS than previously published methods. The method allows up-conce...

journal_title：Proteomics

authors： Zhang X,Shi L,Shu S,Wang Y,Zhao K,Xu N,Liu S,Roepstorff P

更新日期：2007-07-01 00:00:00

abstract：:High-throughput shotgun metaproteomic approaches on environmental or medical microbiomes are producing huge amounts of tandem mass spectrometry data. These can be interpreted either with a general protein sequence database comprising tens of thousands of sequenced genomes or with a more customized database such as tho...

journal_title：Proteomics

authors： Pible O,Armengaud J

更新日期：2015-10-01 00:00:00

abstract：:Immuno-precipitation (IP) experiments using MS provide a sensitive and accurate way of characterising protein complexes and their response to regulatory mechanisms. Differences in stoichiometry can be determined as well as the reliable identification of specific binding partners. The quality control of IP and protein ...

journal_title：Proteomics

authors： ten Have S,Boulon S,Ahmad Y,Lamond AI

更新日期：2011-03-01 00:00:00

abstract：:Quantitative acetyl-proteomics, a newly identified post-translational modification, is known to regulate transcriptional activity in different organisms. Neurospora crassa is a model ascomycete fungus maintained for biochemistry and molecular biology research; however, extensive studies of the functions of its acylati...

journal_title：Proteomics

authors： Wang T,Guan W,Du Y,Xu Y,He Z,Zhang Y,Kang C,Wan X,Chi X,Sun K,Zhang X

更新日期：2021-01-24 00:00:00

abstract：:Cerebrotendinous xanthomatosis (CTX) is an autosomal recessive lipid-storage disorder caused by a deficiency of the mitochondrial sterol 27-hydroxylase. Genetic analysis utilizing SSCP and direct DNA sequencing identified a new mutation. One base-pair of cytosine was deleted at codon 326 on exon 2 of CYP27 in all CTX ...

journal_title：Proteomics

authors： Wang PW,Chang WN,Lu CH,Chao D,Schrag C,Pan TL

更新日期：2006-02-01 00:00:00

abstract：:On 23 and 24 July, 2007, the Centre for Economic and Social Aspects of Genomics (CESAGen) held its first sociomics workshop at the Wellcome Trust Genome Campus, Hinxton, UK. The topic was transformation of knowledge production. Participants included social scientists together with those working on different elements o...

journal_title：Proteomics

authors： McNally R

更新日期：2008-01-01 00:00:00

abstract：:In recent years, standardization and quality control have become important key points in industry, e.g. in drug discovery and for developing medical products. Is quality control in academic Proteomics a minor problem nowadays, where standard data formats and public repositories for data sharing exist? In this article,...

journal_title：Proteomics

authors： Eisenacher M,Schnabel A,Stephan C

更新日期：2011-03-01 00:00:00

abstract：:Current knowledge of the physiology and phylogeny of polycyclic aromatic hydrocarbon (PAH) degrading bacteria often relies on laboratory enrichments and isolations. In the present study, in situ microcosms consisting of activated carbon pellets (BACTRAP®s) were loaded with either (13) C-naphthalene or (13) C-fluorene ...

journal_title：Proteomics

authors： Herbst FA,Bahr A,Duarte M,Pieper DH,Richnow HH,von Bergen M,Seifert J,Bombach P

更新日期：2013-10-01 00:00:00

abstract：:The prediction of adverse drug reactions (ADRs) has become increasingly important, due to the rising concern on serious ADRs that can cause drugs to fail to reach or stay in the market. We proposed a framework for predicting ADR profiles by integrating protein-protein interaction (PPI) networks with drug structures. W...

journal_title：Proteomics

authors： Huang LC,Wu X,Chen JY

更新日期：2013-01-01 00:00:00

abstract：:In this report we introduce a new concept "proteomic trajectory mapping" for the investigation of a complex phenomenon underlying biological transformation and transition. We define proteomic trajectory to be the kinetic trace of protein expression and present a successful proteomic trajectory mapping of complex molec...

journal_title：Proteomics

authors： Haniu H,Komori N,Takemori N,Singh A,Ash JD,Matsumoto H

更新日期：2006-06-01 00:00:00

abstract：:This paper reports on the findings of the Biomedical Research Institute, as one of the participants in the pilot study of the HUPO Brain Proteome Project. A biopsy and autopsy study sample derived from human brain was distributed among the participants for proteomic analysis. In our laboratory, attention was focused o...

journal_title：Proteomics

authors： Dumont D,Noben JP,Verhaert P,Stinissen P,Robben J

更新日期：2006-09-01 00:00:00

abstract：:Leukaemic transformation is frequently associated with the aberrant activity of a protein tyrosine kinase (PTK). As such it is of clinical relevance to be able to map the effects of these leukaemogenic PTKs on haemopoietic cells at the level of phosphorylation modulation. In this paradigm study we have employed a rang...

journal_title：Proteomics

authors： Lee CF,Griffiths S,Rodríguez-Suárez E,Pierce A,Unwin RD,Jaworska E,Evans CA,J Gaskell S,Whetton AD

更新日期：2010-09-01 00:00:00