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abstract：:The thermophilic bacterium Bacillus stearothermophilus P1 is unique in its ability to thrive in extreme environments such as high temperatures or high pH conditions. The study of cold shock response is very interesting and interpreted as a shock response to express the genes involved in synthesis of specific proteins....

journal_title：Proteomics

authors： Sinchaikul S,Sookkheo B,Phutrakul S,Pan FM,Chen ST

更新日期：2002-09-01 00:00:00

abstract：:We present here the first proteomics analysis of uveal melanoma (UM) cells. These cells represent a good model for the identification of polypeptide markers, which could be developed as diagnostic tools. UM is the most common primary intraocular tumour in adults. In contrast to other cancers, the survival rate of pati...

journal_title：Proteomics

authors： Pardo M,García A,Thomas B,Piñeiro A,Akoulitchev A,Dwek RA,Zitzmann N

更新日期：2005-12-01 00:00:00

abstract：:The CpG motif of bacterial DNA (CpG-DNA) is a potent immunostimulating agent whose mechanism of action is not yet clear. Here, we used both DNA microarray and proteomic approaches to investigate the effects of oligodeoxynucleotides containing the CpG motif (CpG-ODN) on gene transcription and protein expression profile...

journal_title：Proteomics

authors： Kuo CC,Kuo CW,Liang CM,Liang SM

更新日期：2005-03-01 00:00:00

abstract：:Many of the emerging technologies for the global evaluation of gene expression, at both the RNA and protein level, are being applied to the problem of finding biomarkers for human disease progression. These analyses can be made difficult, however, by variation between samples that arises from both technical and nondis...

journal_title：Proteomics

authors： Steel LF,Shumpert D,Trotter M,Seeholzer SH,Evans AA,London WT,Dwek R,Block TM

更新日期：2003-05-01 00:00:00

abstract：:Low levels and long term exposure to benzene is associated with hematotoxicity including aplastic anemia, acute myelogenous leukemia, and lymphoma. Current biomonitoring methods such as urinary phenol, S-phenylmercapturic acid, and trans-trans muconic acid were found to be unreliable as analytical methods to detect be...

journal_title：Proteomics

authors： Joo WA,Kang MJ,Son WK,Lee HJ,Lee DY,Lee E,Kim CW

更新日期：2003-12-01 00:00:00

abstract：:A diverse range of proteins are modified by the post-translational covalent attachment of a glycosyl-phosphatidylinositol (GPI) membrane anchor. Hydropathy plots and other computer algorithms can be used to predict the presence of a GPI anchor attachment signal in the nascent polypeptide chain. However, the presence o...

journal_title：Proteomics

authors： Hooper NM

更新日期：2001-06-01 00:00:00

abstract：:Phage display techniques using random peptide interactions have supported the role of mammalian glutathione transferase (GST) as part of a signalling pathway for both oxidative stress and an apoptosis pathway. Little is known about the interaction of nonmammalian GST with other proteins. GSTs have been implicated in t...

journal_title：Proteomics

authors： Greetham D,Morgan C,Campbell AM,van Rossum AJ,Barrett J,Brophy PM

更新日期：2004-07-01 00:00:00

abstract：:Many cancer cells show unique protein expression patterns. We used proteome technology including MS, free flow isoelectric focusing and Western blotting to determine current concentrations of metabolic enzymes in healthy and malignant human thyroid cells. Three different types of human thyroid cells were investigated ...

journal_title：Proteomics

authors： Pietsch J,Sickmann A,Weber G,Bauer J,Egli M,Wildgruber R,Infanger M,Grimm D

更新日期：2012-08-01 00:00:00

abstract：:Oligomeric ubiquitin structures (i.e. ubiquitin "chains") may be formed through any of seven different lysine residues in the polypeptide, or via the amine group of Met 1. Different types of ubiquitin chains can confer very different biological outcomes to a protein substrate, yet the structural characteristics of E2s...

journal_title：Proteomics

authors： Hong JH,Ng D,Srikumar T,Raught B

更新日期：2015-09-01 00:00:00

abstract：:Many types of cells transit in vitro from a two- to a three-dimensional growth, when they are exposed to microgravity. The underlying mechanisms are not yet understood. Hence, we investigated the impact of microgravity on protein content and growth behavior. For this purpose, the human thyroid cancer cells FTC-133 wer...

journal_title：Proteomics

authors： Riwaldt S,Pietsch J,Sickmann A,Bauer J,Braun M,Segerer J,Schwarzwälder A,Aleshcheva G,Corydon TJ,Infanger M,Grimm D

更新日期：2015-09-01 00:00:00

abstract：:Secreted proteins play a pivotal role in cellular functions. To better understand malignant behavior, we adapted stable isotopic labeling with amino acids in cell culture technology to identify and quantify proteins differentially released into the extracellular media by a pair of normal and malignant breast-cancer ce...

journal_title：Proteomics

authors： Liang X,Huuskonen J,Hajivandi M,Manzanedo R,Predki P,Amshey JR,Pope RM

更新日期：2009-01-01 00:00:00

abstract：:In the obese state, as adipose tissue expands, adipocytes become hypoxic and dysfunctional, leading to changes in the pattern of adipocyte-secreted proteins. To better understand the role of hypoxia in the mechanisms linked to obesity, we comparatively analyzed the secretome of murine differentiated 3T3-L1 adipocytes ...

journal_title：Proteomics

authors： Laria AE,Messineo S,Arcidiacono B,Varano M,Chiefari E,Semple RK,Rocha N,Russo D,Cuda G,Gaspari M,Brunetti A,Foti DP

更新日期：2018-04-01 00:00:00

abstract：:In theory, proteases with broad cleavage specificity could be applied to digest protein samples to improve the phosphoproteomic analysis coverage. However, in practice this approach is seldom employed. This is because the identification of phosphopeptides without enzyme specificity by conventional database search stra...

journal_title：Proteomics

authors： Dong M,Ye M,Cheng K,Dong J,Zhu J,Qin H,Bian Y,Zou H

更新日期：2014-11-01 00:00:00

abstract：:MS-based protein identification is an important part of both gel-based and gel-free proteome studies. The MASCOT search engine (http://www.matrixscience.com) provides one of the most popular automated algorithms for this task. Here we present an open-source software library written in Java that parses raw MASCOT resul...

journal_title：Proteomics

authors： Helsens K,Martens L,Vandekerckhove J,Gevaert K

更新日期：2007-02-01 00:00:00

abstract：:The 26S proteasome is a multicatalytic protease complex that degrades ubiquitinated proteins in eukaryotic cells. It consists of a proteolytic core (the 20S proteasome) as well as regulatory particles, which contain six ATPase (Rpt) subunits involved in unfolding and translocation of substrates to the catalytic chambe...

journal_title：Proteomics

authors： Kimura Y,Kurata Y,Ishikawa A,Okayama A,Kamita M,Hirano H

更新日期：2013-11-01 00:00:00

abstract：:Mitochondria play a key role as major regulators of cellular energy homeostasis, but in the context of mitochondrial dysfunction, mitochondria may generate reactive oxidative species and induce cellular apoptosis. Indeed, altered mitochondrial status has been linked to the pathogenesis of several metabolic disorders a...

journal_title：Proteomics

authors： Peinado JR,Diaz-Ruiz A,Frühbeck G,Malagon MM

更新日期：2014-03-01 00:00:00

abstract：:After hind limb suspension, a remodeling of postural muscle phenotype is observed. This remodeling results in a shift of muscle profile from slow-oxidative to fast-glycolytic. These metabolic changes and fiber type shift increase muscle fatigability. Acetyl-L-carnitine (ALCAR) influences the skeletal muscle phenotype ...

journal_title：Proteomics

authors： Moriggi M,Cassano P,Vasso M,Capitanio D,Fania C,Musicco C,Pesce V,Gadaleta MN,Gelfi C

更新日期：2008-09-01 00:00:00

abstract：:We have combined several key sample preparation steps for the use of a liquid matrix system to provide high analytical sensitivity in automated ultraviolet -- matrix-assisted laser desorption/ionisation -- mass spectrometry (UV-MALDI-MS). This new sample preparation protocol employs a matrix-mixture which is based on ...

journal_title：Proteomics

authors： Cramer R,Corless S

更新日期：2005-02-01 00:00:00

abstract：:The sodium (Na+ )-calcium (Ca2+ ) exchanger 1 (NCX1) is an antiporter membrane protein encoded by the SLC8A1 gene. In the heart, it maintains cytosolic Ca2+ homeostasis, serving as the primary mechanism for Ca2+ extrusion during relaxation. Dysregulation of NCX1 is observed in end-stage human heart failure. In this st...

journal_title：Proteomics

authors： Lubelwana Hafver T,Wanichawan P,Manfra O,de Souza GA,Lunde M,Martinsen M,Louch WE,Sejersted OM,Carlson CR

更新日期：2017-09-01 00:00:00

abstract：:Using proteomics in tandem with bioinformatics, the secretomes of nonaggressive and aggressive thyroid carcinoma (TC) cell lines were analyzed to detect potential biomarkers for tumor aggressiveness. A panel of nine proteins, activated leukocyte cell adhesion molecule (ALCAM/CD166), tyrosine-protein kinase receptor (A...

journal_title：Proteomics

authors： Chaker S,Kashat L,Voisin S,Kaur J,Kak I,MacMillan C,Ozcelik H,Siu KW,Ralhan R,Walfish PG

更新日期：2013-03-01 00:00:00

abstract：:Tyrosol (TYR, 2-(4-hydroxyphenyl)ethanol), one of the main phenols in olive oil and olive fruit, significantly strengthens resistance to thermal and oxidative stress in the nematode Caenorhabditis elegans and extends its lifespan. To elucidate the cellular functions regulated by TYR, we have used a proteomic procedure...

journal_title：Proteomics

authors： Cañuelo A,Peragón J

更新日期：2013-10-01 00:00:00

abstract：:Quantitative proteomic studies are contributing greatly to the understanding of the spermatozoon through the provision of detailed information on the proteins spermatozoa acquire and shed in the acquisition of fertility. Extracellular vesicles (EVs) are thought to aid in the delivery of proteins to spermatozoa in the ...

journal_title：Proteomics

authors： Leahy T,Rickard JP,Pini T,Gadella BM,de Graaf SP

更新日期：2020-06-01 00:00:00

abstract：:Pathogenic bacteria excrete a variety of virulence factors into extracellular medium and to the cell surface which have essential roles in the colonization and insurrection of the host cells, and thus reflect the degree of bacterial pathogenicity. For the exploration of virulence factors expressed in the secreted prot...

journal_title：Proteomics

authors： Pocsfalvi G,Cacace G,Cuccurullo M,Serluca G,Sorrentino A,Schlosser G,Blaiotta G,Malorni A

更新日期：2008-06-01 00:00:00

abstract：:The cancer tissue proteome has enormous potential as a source of novel predictive biomarkers in oncology. Progress in the development of mass spectrometry (MS)-based tissue proteomics now presents an opportunity to exploit this by applying the strategies of comprehensive molecular profiling and big-data analytics that...

journal_title：Proteomics

authors： Tully B,Balleine RL,Hains PG,Zhong Q,Reddel RR,Robinson PJ

更新日期：2019-11-01 00:00:00

abstract：:The quantitative analysis of signaling networks requires highly sensitive methods for the time-resolved determination of protein phosphorylation. For this reason, we developed a quantitative protein microarray that monitors the activation of multiple signaling pathways in parallel, and at high temporal resolution. A l...

journal_title：Proteomics

authors： Korf U,Derdak S,Tresch A,Henjes F,Schumacher S,Schmidt C,Hahn B,Lehmann WD,Poustka A,Beissbarth T,Klingmüller U

更新日期：2008-11-01 00:00:00

abstract：:Due to their extraordinary capacity to hypertolerate and hyperaccumulate heavy metals in above-ground tissues, hyperaccumulator plant species have gained wide attention from researchers seeking biotechnologies to manage environmental heavy metal pollution. However, the molecular basis of hyperaccumulation is still far...

journal_title：Proteomics

authors： Zhang Z,Zhou H,Yu Q,Li Y,Mendoza-Cózatl DG,Qiu B,Liu P,Chen Q

更新日期：2017-05-01 00:00:00

abstract：:By mining publicly accessible databases, we have developed a collection of tissue-specific predictive protein expression maps as a function of cancer histological state. Data analysis is applied to the differential expression of gene products in pooled libraries from the normal to the altered state(s). We wish to repo...

journal_title：Proteomics

authors： Medjahed D,Luke BT,Tontesh TS,Smythers GW,Munroe DJ,Lemkin PF

更新日期：2003-08-01 00:00:00

abstract：:Antibodies that recognize PTMs of histones play a central role in epigenetic proteomic research. Modification-specific antibodies are employed in chromatin immunoprecipitation, for Western blotting and during the immunoprecipitation steps for MS-based global proteomic analyses. Knowledge about the antibodies' off-targ...

journal_title：Proteomics

authors： Heubach Y,Planatscher H,Sommersdorf C,Maisch D,Maier J,Joos TO,Templin MF,Poetz O

更新日期：2013-03-01 00:00:00

abstract：:Sea star coelomic fluid is in contact with all internal organs, carrying signaling molecules and a large population of circulating cells, the coelomocytes. These cells, also known as echinoderm blood cells, are responsible for the innate immune responses and are also known to have an important role in the first stage ...

journal_title：Proteomics

authors： Franco CF,Santos R,Coelho AV

更新日期：2011-09-01 00:00:00

abstract：:To gain a better understanding of salt stress responses in plants, we used a proteomic approach to investigate changes in rice (Oryza sativa) root plasma-membrane-associated proteins following treatment with 150 mmol/L NaCl. With or without a 48 h salt treatment, plasma membrane fractions from root tip cells of a salt...

journal_title：Proteomics

authors： Cheng Y,Qi Y,Zhu Q,Chen X,Wang N,Zhao X,Chen H,Cui X,Xu L,Zhang W

更新日期：2009-06-01 00:00:00