Abstract:
:Sea star coelomic fluid is in contact with all internal organs, carrying signaling molecules and a large population of circulating cells, the coelomocytes. These cells, also known as echinoderm blood cells, are responsible for the innate immune responses and are also known to have an important role in the first stage of regeneration, i.e. wound closure, necessary to prevent disruption of the body fluid balance and to limit the invasion of pathogens. This study focuses on the proteome characterization of these multifunctional cells. The identification of 358 proteins was achieved using a combination of two techniques for protein separation (1-D SDS-PAGE followed by nanoLC and 2-D SDS-PAGE) and MALDI-TOF/TOF MS for protein identification. To our knowledge, the present report represents the first comprehensive list of sea star coelomocyte proteins, constituting an important database to validate many echinoderm-predicted proteins. Evidence for new pathways in these particular echinoderm cells are also described, and thus representing a valuable resource to stimulate future studies aiming to unravel the homology with vertebrate immune cells and particularly the origins of the immune system itself.
journal_name
Proteomicsjournal_title
Proteomicsauthors
Franco CF,Santos R,Coelho AVdoi
10.1002/pmic.201000745subject
Has Abstractpub_date
2011-09-01 00:00:00pages
3587-92issue
17eissn
1615-9853issn
1615-9861journal_volume
11pub_type
杂志文章相关文献
PROTEOMICS文献大全abstract::We have developed a surface plasmon resonance (SPR)-based protein microarray to study protein-protein interactions in a high-throughput mode. As a model system, triple protein interactions have been explored with human papillomaviral E6 protein, tumor suppressor p53, and ubiquitin ligase E6AP. Human papillomavirus (HP...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200500635
更新日期:2006-04-01 00:00:00
abstract::This review provides a brief overview of the development of data-independent acquisition (DIA) mass spectrometry-based proteomics and selected DIA data analysis tools. Various DIA acquisition schemes for proteomics are summarized first including Shotgun-CID, DIA, MSE , PAcIFIC, AIF, SWATH, MSX, SONAR, WiSIM, BoxCar, S...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.201900276
更新日期:2020-09-01 00:00:00
abstract::Canonical isoforms in different databases have been defined as the most prevalent, most conserved, most expressed, longest, or the one with the clearest description of domains or posttranslational modifications. In this article, we revisit these definitions of canonical isoforms based on functional genomics and proteo...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201400170
更新日期:2014-12-01 00:00:00
abstract::Atopic dermatitis (AD) is a chronic relapsing inflammatory skin disease typically characterized by a distribution of eczematous skin lesions with lichenification, pruritic excoriations, and dry skin with wide varieties of pathophysiologic aspects. Recently, AD was divided into extrinsic and intrinsic forms according t...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200400998
更新日期:2004-11-01 00:00:00
abstract::Biology is increasingly dependent on large-scale analysis, such as proteomics, creating a requirement for efficient bioinformatics. Bioinformatic predictions of biological functions rely upon correctly annotated database sequences, and the presence of inaccurately annotated or otherwise poorly described sequences intr...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201600034
更新日期:2016-09-01 00:00:00
abstract::Sequencing of the human genome opened the way to the exploration of the proteome and this has lead to the identification of large numbers of proteins in complex biological samples. The identification of diagnostic patterns in samples taken from patients to aid diagnosis is in the early stages of development. The solut...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.200700665
更新日期:2008-02-01 00:00:00
abstract::Protein ubiquitination is a powerful modulator of cellular functions. Classically linked to the degradation of proteins, it also plays a role in intracellular localization, DNA damage response, vesicle fusion events, and the immune and transcriptional responses. Ubiquitin is versatile and can code for several distinct...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.201500290
更新日期:2016-01-01 00:00:00
abstract::In this work, we first immobilized tin(IV) ion on polydopamine-coated magnetic graphene (magG@PDA) to synthesize Sn4+ -immobilized magG@PDA (magG@PDA-Sn4+ ) and successfully applied the material to highly selective enrichment of phosphopeptides. The material gathered the advantages of large surface area of graphene, s...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201600187
更新日期:2016-11-01 00:00:00
abstract::Deciphering a complete landscape of protein-RNA interactions in the human proteome remains an elusive challenge. We computationally elucidate RNA binding proteins (RBPs) using an approach that complements previous efforts. We employ two modern complementary sequence-based methods that provide accurate predictions from...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201800064
更新日期:2018-11-01 00:00:00
abstract::Mitochondria consist of four compartments, outer membrane, intermembrane space, inner membrane, and matrix; each harboring specific functions and structures. In this study, we used LC-MS/MS to characterize the protein composition of Trypanosoma brucei mitochondrial (mt) membranes, which were enriched by different bioc...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200900354
更新日期:2009-12-01 00:00:00
abstract::Since drug resistance is a complex and multifactorial event involving activation/repression of multiple biochemical pathways, we used a proteomic approach to study cisplatin resistance and drug response in human tumor cell lines. The cervix squamous cell carcinoma cell line A431 and its cisplatin-resistant subline, A4...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200400835
更新日期:2004-10-01 00:00:00
abstract::Long-term storage of protein samples for transportation is a challenge in the field of mass spectrometric analysis because the low temperature condition is not easy to be maintained. Here we introduce a simple method to preserve proteins at room temperature for at least one month. In this method, the protein sample is...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201700336
更新日期:2018-07-01 00:00:00
abstract::The transfection of glycoprotein glycosyltransferase genes into cells leads to modification of both the structure and function of the glycoproteins and as a result, changes in glycome patterns. N-glycan branching enzymes hold some promise as a model system for the identification of glycome patterns. Both N-acetylgluco...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/1615-9861(200102)1:2<239::AID-PROT239>3.0.
更新日期:2001-02-01 00:00:00
abstract::Mitochondria play a key role as major regulators of cellular energy homeostasis, but in the context of mitochondrial dysfunction, mitochondria may generate reactive oxidative species and induce cellular apoptosis. Indeed, altered mitochondrial status has been linked to the pathogenesis of several metabolic disorders a...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.201300376
更新日期:2014-03-01 00:00:00
abstract::CRISPR-Cas gene editing holds substantial promise in many biomedical disciplines and basic research. Due to the important functional implications of non-histone chromosomal protein HMG-14 (HMGN1) in regulating chromatin structure and tumor immunity, gene knockout of HMGN1 is performed by CRISPR in cancer cells and the...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201800438
更新日期:2019-07-01 00:00:00
abstract::Absolute protein concentration determination is becoming increasingly important in a number of fields including diagnostics, biomarker discovery and systems biology modeling. The recently introduced quantification concatamer methodology provides a novel approach to performing such determinations, and it has been appli...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201000454
更新日期:2011-01-01 00:00:00
abstract::Proteolysis affects every protein at some point in its life cycle. Many biomarkers of disease or cancer are stable proteolytic fragments in biological fluids. There is great interest and a challenge in proteolytically modified protein study to identify physiologic protease-substrate relationships and find potential bi...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201200027
更新日期:2012-06-01 00:00:00
abstract::The field of biochemistry is currently faced with the enormous challenge of assigning functional significance to more than thirty thousand predicted protein products encoded by the human genome. In order to accomplish this daunting task, methods will be required that facilitate the global analysis of proteins in compl...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/1615-9861(200109)1:9<1067::AID-PROT1067>3.
更新日期:2001-09-01 00:00:00
abstract::Lectin microarray is an emerging technique, which will accelerate glycan profiling and discovery of glycan-related biomarkers. One of the most important stages in realizing the potential of the technique is to achieve sufficiently high sensitivity to detect even the low concentrations of some target glycoproteins whic...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200701114
更新日期:2008-08-01 00:00:00
abstract::A method for quantitative proteomic analysis based on the selective isolation of multiply charged peptides (RH peptides) containing arginine and histidine residues is described. Two pools of proteins are digested in tandem with lysyl-endopeptidase and trypsin and the primary amino groups of proteolytic peptides are se...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200500836
更新日期:2006-08-01 00:00:00
abstract::An improved method for peptide sequencing based on acetylation/deuteroacetylation in conjunction with ESI MS is introduced. Derivatization with a 1:1 mixture of acetic anhydride and deuterated acetic anhydride incorporates a stable isotope label into the analyzed molecule. This approach has been initially applied to F...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200401319
更新日期:2005-11-01 00:00:00
abstract::The Ser/Thr/Tyr phosphoproteome of Bacillus subtilis was analyzed by a 2-D gel-based approach combining Pro-Q Diamond staining and [(33)P]-labeling. In exponentially growing B. subtilis cells 27 proteins could be identified after staining with Pro-Q Diamond and/or [(33)P]-labeling and one additional protein was labele...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200700232
更新日期:2007-10-01 00:00:00
abstract::The E7 protein produced by high-risk human papillomavirus (HPV) induces a degradation of the retinoblastoma tumor suppressor RB through direct interaction, which suggests that an inhibitor for the interaction can be a potential anticancer drug. A surface plasmon resonance (SPR) imaging-based protein array chip was dev...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200500001
更新日期:2005-11-01 00:00:00
abstract::Immobilized metal affinity chromatography (IMAC) is a commonly used technique for phosphoproteome analysis due to its high affinity for adsorption of phosphopeptides. Miniaturization of IMAC column is essential for the analysis of a small amount of sample. Nanoscale IMAC column was prepared by chemical modification of...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200600045
更新日期:2007-02-01 00:00:00
abstract::In an effort to identify tumor-associated proteins from plasma of tumor-bearing mice that may be used as diagnostic biomarkers, we developed a strategy that combines a tumor xenotransplantation model in nude mice with comparative proteomic technology. Five human cancer cell lines (SC-M1, HONE-1, CC-M1, OECM1, GBM 8401...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200400785
更新日期:2004-09-01 00:00:00
abstract::Previous studies have found that some first onset schizophrenia patients show signs of impaired insulin signaling. Also, epidemiological studies have shown that periods of suboptimal nutrition including protein deficiencies during pregnancy can lead to increased incidence of metabolic conditions and psychiatric disord...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201200376
更新日期:2012-12-01 00:00:00
abstract::Aging is associated with a progressive loss of skeletal muscular function that often leads to progressive disability and loss of independence. Although muscle aging is well documented, the molecular mechanisms of this condition still remain unclear. To gain greater insight into the changes associated with aging of ske...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201200497
更新日期:2014-01-01 00:00:00
abstract::Long-lived conifer trees depend on both constitutive and induced defenses for resistance against a myriad of potential pathogens and herbivores. In species of spruce (Picea spp.), several of the late events of pathogen-, insect-, or elicitor-induced defense responses have previously been characterized at the anatomica...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200800252
更新日期:2009-01-01 00:00:00
abstract::Acceleration of liver regeneration could be of great clinical benefit in various liver-associated diseases. However, at present little is known about therapeutic interventions to enhance this regenerative process. Our limited understanding and the complexity of the mechanisms involved have prevented the identification...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200400913
更新日期:2005-01-01 00:00:00
abstract::The severe pulmonary form of leptospirosis (SPFL) is an especially serious and rapid disease process characterized by alveolar hemorrhage and acute respiratory failure. The outer membrane of Leptospira facilitates direct interactions with the environs and likely contains important constituents involved during infectio...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200400880
更新日期:2005-01-01 00:00:00