Abstract:
:Antibodies that recognize PTMs of histones play a central role in epigenetic proteomic research. Modification-specific antibodies are employed in chromatin immunoprecipitation, for Western blotting and during the immunoprecipitation steps for MS-based global proteomic analyses. Knowledge about the antibodies' off-target binding is essential for the interpretation of experimental data. To address this challenge we developed a fast and cost efficient system for generating peptide bead arrays. We employed this method to establish a bead-based peptide array containing 384 peptides displaying phosphorylated, acetylated, methylated, and citrullinated N-terminal regions of histones H2A, H2B, H3 and H4 and controls. We profiled the binding of 40 PTM-specific antibodies important for epigenetic proteomic research.
journal_name
Proteomicsjournal_title
Proteomicsauthors
Heubach Y,Planatscher H,Sommersdorf C,Maisch D,Maier J,Joos TO,Templin MF,Poetz Odoi
10.1002/pmic.201200383subject
Has Abstractpub_date
2013-03-01 00:00:00pages
1010-5issue
6eissn
1615-9853issn
1615-9861journal_volume
13pub_type
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