iTRAQ-based quantitative proteomic analysis reveals new metabolic pathways of wheat seedling growth under hydrogen peroxide stress.


:As an abundant ROS, hydrogen peroxide (H2 O2 ) plays pivotal roles in plant growth and development. In this work, we conducted for the first time an iTRAQ-based quantitative proteomic analysis of wheat seedling growth under different exogenous H2 O2 treatments. The growth of seedlings and roots was significantly restrained by increased H2 O2 concentration stress. Malondialdehyde, soluble sugar, and proline contents as well as peroxidase activity increased with increasing H2 O2 levels. A total of 3,425 proteins were identified by iTRAQ, of which 157 showed differential expression and 44 were newly identified H2 O2 -responsive proteins. H2 O2 -responsive proteins were mainly involved in stress/defense/detoxification, signal transduction, and carbohydrate metabolism. It is clear that up-regulated expression of signal transduction and stress/defence/detoxification-related proteins under H2 O2 stress, such as plasma membrane intrinsic protein 1, fasciclin-like arabinogalactan protein, and superoxide dismutase, could contribute to H2 O2 tolerance of wheat seedlings. Increased gluconeogenesis (phosphoenol-pyruvate carboxykinase) and decreased pyruvate kinase proteins are potentially related to the higher H2 O2 tolerance of wheat seedlings. A metabolic pathway of wheat seedling growth under H2 O2 stress is presented.






Ge P,Hao P,Cao M,Guo G,Lv D,Subburaj S,Li X,Yan X,Xiao J,Ma W,Yan Y




Has Abstract


2013-10-01 00:00:00












  • Towards standard protocols and guidelines for urine proteomics: a report on the Human Kidney and Urine Proteome Project (HKUPP) symposium and workshop, 6 October 2007, Seoul, Korea and 1 November 2007, San Francisco, CA, USA.

    abstract::The Human Kidney and Urine Proteome Project (HKUPP) was initiated in 2005 to promote proteomics research in the nephrology field, to better understand kidney functions as well as pathogenic mechanisms of kidney diseases, and to define novel biomarkers and therapeutic targets. This project was first approved in 2005 by...




    authors: Yamamoto T,Langham RG,Ronco P,Knepper MA,Thongboonkerd V

    更新日期:2008-06-01 00:00:00

  • The proteomics of senescence in leaves of white clover, Trifolium repens (L.).

    abstract::A proteomics approach has been used to study changes in protein abundance during leaf senescence in white clover. Changes in cell ultrastructure were also examined using transmission electron microscopy. The most obvious ultrastructural changes during senescence occurred in chloroplasts, with progressive loss of thyla...


    pub_type: 杂志文章


    authors: Wilson KA,McManus MT,Gordon ME,Jordan TW

    更新日期:2002-09-01 00:00:00

  • Proteomic analysis of interactors for yeast protein arginine methyltransferase Hmt1 reveals novel substrate and insights into additional biological roles.

    abstract::Protein arginine methylation is a PTM catalyzed by an evolutionarily conserved family of enzymes called protein arginine methyltransferases (PRMTs), with PRMT1 being the most conserved member of this enzyme family. This modification has emerged to be an important regulator of protein functions. To better understand th...


    pub_type: 杂志文章


    authors: Jackson CA,Yadav N,Min S,Li J,Milliman EJ,Qu J,Chen YC,Yu MC

    更新日期:2012-11-01 00:00:00

  • Requirements for the valid quantification of immunostains on tissue microarray materials using image analysis.

    abstract::Antibody-based proteomics applied to tissue microarray (TMA) technology provides a very efficient means of visualizing and locating antigen expression in large collections of normal and pathological tissue samples. To characterize antigen expression on TMAs, the use of image analysis methods avoids the effects of huma...


    pub_type: 杂志文章


    authors: Decaestecker C,Lopez XM,D'Haene N,Roland I,Guendouz S,Duponchelle C,Berton A,Debeir O,Salmon I

    更新日期:2009-10-01 00:00:00

  • A structure-based strategy for discovery of small ligands binding to functionally unknown proteins: combination of in silico screening and surface plasmon resonance measurements.

    abstract::In the postgenomic era, many researchers and organizations have been engaged in structural and functional analyses of proteins. As a part of these efforts, searching for small organic compounds that bind specifically to target proteins is quite important. In this study, we have developed a rational strategy for ligand...


    pub_type: 杂志文章


    authors: Yoshitani N,Satou K,Saito K,Suzuki S,Hatanaka H,Seki M,Shinozaki K,Hirota H,Yokoyama S

    更新日期:2005-04-01 00:00:00

  • Purification of mRNA-programmed translation initiation complexes suitable for mass spectrometry analysis.

    abstract::Liquid Chromatography coupled to tandem mass spectrometry (nanoLC-MS/MS) is a powerful analytical technique for the identification and mass analysis of complex protein mixtures. Here, we present a combination of methods developed for the extensive/deep proteomic analysis of purified ribosome/mRNA particles assembled i...


    pub_type: 杂志文章


    authors: Chicher J,Simonetti A,Kuhn L,Schaeffer L,Hammann P,Eriani G,Martin F

    更新日期:2015-07-01 00:00:00

  • 2-D DIGE analysis of the mitochondrial proteome from human skeletal muscle reveals time course-dependent remodelling in response to 14 consecutive days of endurance exercise training.

    abstract::Adaptation of skeletal muscle to repeated bouts of endurance exercise increases aerobic capacity and improves mitochondrial function. However, the adaptation of human skeletal muscle mitochondrial proteome to short-term endurance exercise training has not been investigated. Eight sedentary males cycled for 60 min at 8...


    pub_type: 杂志文章


    authors: Egan B,Dowling P,O'Connor PL,Henry M,Meleady P,Zierath JR,O'Gorman DJ

    更新日期:2011-04-01 00:00:00

  • Identification of FBXO25-interacting proteins using an integrated proteomics approach.

    abstract::FBXO25 is one of the 68 human F-box proteins that serve as specificity factors for a family of ubiquitin ligases composed of s-phase-kinase associated protein 1, really interesting new gene-box 1, Cullin 1, and F-box protein (SCF1) that are involved in targeting proteins for destruction across the ubiquitin proteasome...


    pub_type: 杂志文章


    authors: Teixeira FR,Yokoo S,Gartner CA,Manfiolli AO,Baqui MM,Assmann EM,Maragno AL,Yu H,de Lanerolle P,Kobarg J,Gygi SP,Gomes MD

    更新日期:2010-08-01 00:00:00

  • Surface plasmon resonance imaging-based protein arrays for high-throughput screening of protein-protein interaction inhibitors.

    abstract::The E7 protein produced by high-risk human papillomavirus (HPV) induces a degradation of the retinoblastoma tumor suppressor RB through direct interaction, which suggests that an inhibitor for the interaction can be a potential anticancer drug. A surface plasmon resonance (SPR) imaging-based protein array chip was dev...


    pub_type: 杂志文章


    authors: Jung SO,Ro HS,Kho BH,Shin YB,Kim MG,Chung BH

    更新日期:2005-11-01 00:00:00

  • Addressing the Challenges of High-Throughput Cancer Tissue Proteomics for Clinical Application: ProCan.

    abstract::The cancer tissue proteome has enormous potential as a source of novel predictive biomarkers in oncology. Progress in the development of mass spectrometry (MS)-based tissue proteomics now presents an opportunity to exploit this by applying the strategies of comprehensive molecular profiling and big-data analytics that...


    pub_type: 杂志文章


    authors: Tully B,Balleine RL,Hains PG,Zhong Q,Reddel RR,Robinson PJ

    更新日期:2019-11-01 00:00:00

  • An improved mechanically durable electrophoresis gel matrix that is fully compatible with fluorescence-based protein detection technologies.

    abstract::Unfortunately, conventional large-format polyacrylamide gels are mechanically fragile, often tearing during the subsequent manipulations required for visualization of the proteins. This problem is compounded when large-format two-dimensional gels are subjected to multiple staining procedures in order to detect differe...


    pub_type: 杂志文章


    authors: Schulenberg B,Arnold B,Patton WF

    更新日期:2003-07-01 00:00:00

  • The human embryonic stem cell proteome revealed by multidimensional fractionation followed by tandem mass spectrometry.

    abstract::Human embryonic stem cells (hESCs) have received considerable attention due to their therapeutic potential and usefulness in understanding early development and cell fate commitment. In order to appreciate the unique properties of these pluripotent, self-renewing cells, we have performed an in-depth multidimensional f...


    pub_type: 杂志文章


    authors: Zhao P,Schulz TC,Sherrer ES,Weatherly DB,Robins AJ,Wells L

    更新日期:2015-01-01 00:00:00

  • Immobilization strategies for single-chain antibody microarrays.

    abstract::Sandwich ELISA microarrays have great potential for validating disease biomarkers. Each ELISA relies on robust-affinity reagents that retain activity when immobilized on a solid surface or when labeled for detection. Single-chain antibodies (scFv) are affinity reagents that have greater potential for high-throughput p...


    pub_type: 杂志文章


    authors: Seurynck-Servoss SL,Baird CL,Miller KD,Pefaur NB,Gonzalez RM,Apiyo DO,Engelmann HE,Srivastava S,Kagan J,Rodland KD,Zangar RC

    更新日期:2008-06-01 00:00:00

  • Proteomic analysis of membrane microdomains derived from both failing and non-failing human hearts.

    abstract::Eukaryotic cells plasma membranes are organized into microdomains of specialized function such as lipid rafts and caveolae, with a specific lipid composition highly enriched in cholesterol and glycosphingolipids. In addition to their role in regulating signal transduction, multiple functions have been proposed, such a...


    pub_type: 杂志文章


    authors: Banfi C,Brioschi M,Wait R,Begum S,Gianazza E,Fratto P,Polvani G,Vitali E,Parolari A,Mussoni L,Tremoli E

    更新日期:2006-03-01 00:00:00

  • Metabolomics Integrated with Transcriptomics Reveals a Subtle Liver Metabolic Risk in Dairy Cows Fed Different Crop By-products.

    abstract::Ruminants make large contributions to sustainable agriculture by converting crop by-products into agricultural food. Multi-omics integrative analysis helps to uncover the underlying molecular mechanisms. The liver metabolome-transcriptome interface (LMTI) in dairy cows, including 3938 significant correlations (p < 0.0...


    pub_type: 杂志文章


    authors: Sun HZ,Wang DM,Liu HY,Liu JX

    更新日期:2018-08-01 00:00:00

  • Quantitative Proteomic Analysis of Staphylococcus aureus Treated With Punicalagin, a Natural Antibiotic From Pomegranate That Disrupts Iron Homeostasis and Induces SOS.

    abstract::Staphylococcus aureus, a bacterial, food-borne pathogen of humans, can contaminate raw fruits and vegetables. While physical and chemical methods are available to control S. aureus, scientists are searching for inhibitory phytochemicals from plants. One promising compound from pomegranate is punicalagin, a natural ant...


    pub_type: 杂志文章


    authors: Cooper B,Islam N,Xu Y,Beard HS,Garrett WM,Gu G,Nou X

    更新日期:2018-05-01 00:00:00

  • Comparative proteomic analysis of trophoblast cell models reveals their differential phenotypes, potential uses, and limitations.

    abstract::Trophoblastic cell lines are widely used in in vitro studies of placental function as a surrogate for primary trophoblasts. To date, no reference proteomics dataset exists to directly compare the shared and unique characteristics of these cells. Here, we performed comparative proteomic profiling of the BeWo and HTR8/S...


    pub_type: 杂志文章


    authors: Szklanna PB,Wynne K,Nolan M,Egan K,Áinle FN,Maguire PB

    更新日期:2017-05-01 00:00:00

  • High-sensitivity HLA class I peptidome analysis enables a precise definition of peptide motifs and the identification of peptides from cell lines and patients' sera.

    abstract::The characterization of peptides bound to human leukocyte antigen (HLA) class I is of fundamental importance for understanding CD8+ T cell-driven immunological processes and for the development of immunomodulatory therapeutic strategies. However, until now, the mass spectrometric analysis of HLA-bound peptides has typ...


    pub_type: 杂志文章


    authors: Ritz D,Gloger A,Weide B,Garbe C,Neri D,Fugmann T

    更新日期:2016-05-01 00:00:00

  • OMSSAGUI: An open-source user interface component to configure and run the OMSSA search engine.

    abstract::We here present a user-friendly and extremely lightweight tool that can serve as a stand-alone front-end for the Open MS Search Algorithm (OMSSA) search engine, or that can directly be used as part of an informatics processing pipeline for MS driven proteomics. The OMSSA graphical user interface (OMSSAGUI) tool is wri...


    pub_type: 杂志文章


    authors: Tharakan R,Martens L,Van Eyk JE,Graham DR

    更新日期:2008-06-01 00:00:00

  • Time-dependent evolution of tissue markers by MALDI-MS imaging.

    abstract::We have used MALDI-MS imaging (MALDI-MSI) to monitor the time dependent appearance and loss of signals when tissue slices are brought rapidly to room temperature for short to medium periods of time. Sections from mouse brain were cut in a cryostat microtome, placed on a MALDI target and allowed to warm to room tempera...


    pub_type: 杂志文章


    authors: Goodwin RJ,Dungworth JC,Cobb SR,Pitt AR

    更新日期:2008-09-01 00:00:00

  • Identification of human nasal mucous proteins using proteomics.

    abstract::The determination of possible biomarkers in nasal secretion of healthy subjects can have a role in early diagnosis of diseases such as rhinosinusitis. For this purpose, nasal lavage fluids (NLFs) from ten volunteers, collected before and after they had been submitted to nasal provocations, were investigated. Separatio...


    pub_type: 杂志文章


    authors: Casado B,Pannell LK,Iadarola P,Baraniuk JN

    更新日期:2005-07-01 00:00:00

  • DeepQuanTR: MALDI-MS-based label-free quantification of proteins in complex biological samples.

    abstract::The quantification of changes in protein abundance in complex biological specimens is essential for proteomic studies in basic and applied research. Here we report on the development and validation of the DeepQuanTR software for identification and quantification of differentially expressed proteins using LC-MALDI-MS. ...


    pub_type: 杂志文章


    authors: Fugmann T,Neri D,Roesli C

    更新日期:2010-07-01 00:00:00

  • Proteome analysis of Escherichia coli using high-performance liquid chromatography and Fourier transform ion cyclotron resonance mass spectrometry.

    abstract::The basic problem of complexity poses a significant challenge for proteomic studies. To date two-dimensional gel electrophoresis (2-DE) followed by enzymatic in-gel digestion of the peptides, and subsequent identification by mass spectrometry (MS) is the most commonly used method to analyze complex protein mixtures. H...


    pub_type: 杂志文章


    authors: Ihling C,Sinz A

    更新日期:2005-05-01 00:00:00

  • Difference gel electrophoresis.

    abstract::DIGE is a protein labelling and separation technique allowing quantitative proteomics of two or more samples by optical fluorescence detection of differentially labelled proteins that are electrophoretically separated on the same gel. DIGE is an alternative to quantitation by MS-based methodologies and can circumvent ...


    pub_type: 杂志文章,评审


    authors: Timms JF,Cramer R

    更新日期:2008-12-01 00:00:00

  • "You produce while I clean up", a strategy revealed by exoproteomics during Synechococcus-Roseobacter interactions.

    abstract::Most of the energy that is introduced into the oceans by photosynthetic primary producers is in the form of organic matter that then sustains the rest of the food web, from micro to macro-organisms. However, it is the interactions between phototrophs and heterotrophs that are vital to maintaining the nutrient balance ...


    pub_type: 杂志文章


    authors: Christie-Oleza JA,Scanlan DJ,Armengaud J

    更新日期:2015-10-01 00:00:00

  • Albumin depletion of human plasma also removes low abundance proteins including the cytokines.

    abstract::The use of proteomics for efficient, accurate, and complete analysis of clinical samples poses a variety of technical challenges. The presence of higher abundance proteins in the plasma, such as albumin, may mask the detection of lower abundance proteins such as the cytokines. Methods have been proposed to deplete the...


    pub_type: 杂志文章


    authors: Granger J,Siddiqui J,Copeland S,Remick D

    更新日期:2005-12-01 00:00:00

  • Improving the quality of genome, protein sequence, and taxonomy databases: a prerequisite for microbiome meta-omics 2.0.

    abstract::High-throughput shotgun metaproteomic approaches on environmental or medical microbiomes are producing huge amounts of tandem mass spectrometry data. These can be interpreted either with a general protein sequence database comprising tens of thousands of sequenced genomes or with a more customized database such as tho...


    pub_type: 杂志文章


    authors: Pible O,Armengaud J

    更新日期:2015-10-01 00:00:00

  • Structural analysis of a highly acetylated protein using a curved-field reflectron mass spectrometer.

    abstract::Matrix-assisted laser desorption/ionization mass spectrometry and tandem mass spectrometry (MS/MS) were used to determine the multiple acetylation sites in the histone acetyltransferase (HAT): p300-HAT. Partial cleavage of the peptides containing acetylated lysine residues by trypsin provided a set of nested sequences...


    pub_type: 杂志文章


    authors: Wang D,Thompson P,Cole PA,Cotter RJ

    更新日期:2005-06-01 00:00:00

  • PathwayPCA: an R/Bioconductor Package for Pathway Based Integrative Analysis of Multi-Omics Data.

    abstract::The authors present pathwayPCA, an R/Bioconductor package for integrative pathway analysis that utilizes modern statistical methodology, including supervised and adaptive, elastic-net, sparse principal component analysis. pathwayPCA can be applied to continuous, binary, and survival outcomes in studies with multiple c...


    pub_type: 杂志文章


    authors: Odom GJ,Ban Y,Colaprico A,Liu L,Silva TC,Sun X,Pico AR,Zhang B,Wang L,Chen X

    更新日期:2020-11-01 00:00:00

  • Digital microfluidic hydrogel microreactors for proteomics.

    abstract::Proteolytic digestion is an essential step in proteomic sample processing. While this step has traditionally been implemented in homogeneous (solution) format, there is a growing trend to use heterogeneous systems in which the enzyme is immobilized on hydrogels or other solid supports. Here, we introduce the use of im...


    pub_type: 杂志文章


    authors: Luk VN,Fiddes LK,Luk VM,Kumacheva E,Wheeler AR

    更新日期:2012-05-01 00:00:00