Ligand-independent orphan receptor TR2 activation by phosphorylation at the DNA-binding domain.


:In a previous report we demonstrated protein kinase C (PKC)-mediated phosphorylation of the ligand-binding domain (LBD) of orphan nuclear receptor TR2. In this report, we provide the evidence of PKC-mediated phosphorylation of the DNA-binding domain (DBD) of TR2. Two PKC target sites were predicted within the DBD, at Ser-170 and Ser-185, but only Ser-185 was confirmed by MS. Phosphorylation of DBD facilitated DNA binding of the TR2 receptor and its recruiting of coactivator p300/CBP-associated factor (P/CAF). Ser-185 was required for DNA binding, whereas both Ser-170 and Ser-185 were necessary for receptor interaction with P/CAF. The P/CAF-interacting domain of TR2 was located in its DBD. A double mutant (Ser-170 and Ser-185) of TR2 significantly lowered the activation of its target gene RARbeta2. This study provides the first evidence for ligand-independent activation of TR2 orphan receptor through PTM at the DBD, which enhanced its DNA-binding ability and interaction with coactivator P/CAF.






Khan SA,Park SW,Huq MD,Wei LN




Has Abstract


2006-01-01 00:00:00












  • Incorporating high-throughput proteomics experiments into structural biology pipelines: identification of the low-hanging fruits.

    abstract::The last years have seen the emergence of many large-scale proteomics initiatives that have identified thousands of new protein interactions and macromolecular assemblies. However, unfortunately, only a few among the discovered complexes meet the high-quality standards required to be promptly used in structural studie...


    pub_type: 杂志文章


    authors: Pache RA,Aloy P

    更新日期:2008-05-01 00:00:00

  • Large-scale identification of proteins in human salivary proteome by liquid chromatography/mass spectrometry and two-dimensional gel electrophoresis-mass spectrometry.

    abstract::Human saliva contains a large number of proteins and peptides (salivary proteome) that help maintain homeostasis in the oral cavity. Global analysis of human salivary proteome is important for understanding oral health and disease pathogenesis. In this study, large-scale identification of salivary proteins was demonst...


    pub_type: 杂志文章


    authors: Hu S,Xie Y,Ramachandran P,Ogorzalek Loo RR,Li Y,Loo JA,Wong DT

    更新日期:2005-04-01 00:00:00

  • Evaluation of three-dimensional gel electrophoresis to improve quantitative profiling of complex proteomes.

    abstract::Two-dimensional remains one of the main experimental approaches in proteome analysis. However, comigration of protein leads to several limitations: lack of accuracy in protein identification, impaired comparative quantification, and PTM detection. We have optimized a third additional step of in-gel separation to allev...


    pub_type: 杂志文章


    authors: Colignon B,Raes M,Dieu M,Delaive E,Mauro S

    更新日期:2013-07-01 00:00:00

  • Proteomic study of human umbilical vein endothelial cells in culture.

    abstract::The endothelium is a single layer of cells lining the inside face of all blood vessels. It constitutes a major metabolic organ which is critically involved in the generation and the regulation of multiple physiological and pathological processes such as coagulation, hemostasis, inflammation, atherosclerosis, angiogene...


    pub_type: 杂志文章


    authors: Bruneel A,Labas V,Mailloux A,Sharma S,Vinh J,Vaubourdolle M,Baudin B

    更新日期:2003-05-01 00:00:00

  • Towards standard protocols and guidelines for urine proteomics: a report on the Human Kidney and Urine Proteome Project (HKUPP) symposium and workshop, 6 October 2007, Seoul, Korea and 1 November 2007, San Francisco, CA, USA.

    abstract::The Human Kidney and Urine Proteome Project (HKUPP) was initiated in 2005 to promote proteomics research in the nephrology field, to better understand kidney functions as well as pathogenic mechanisms of kidney diseases, and to define novel biomarkers and therapeutic targets. This project was first approved in 2005 by...




    authors: Yamamoto T,Langham RG,Ronco P,Knepper MA,Thongboonkerd V

    更新日期:2008-06-01 00:00:00

  • New changes in the plasma-membrane-associated proteome of rice roots under salt stress.

    abstract::To gain a better understanding of salt stress responses in plants, we used a proteomic approach to investigate changes in rice (Oryza sativa) root plasma-membrane-associated proteins following treatment with 150 mmol/L NaCl. With or without a 48 h salt treatment, plasma membrane fractions from root tip cells of a salt...


    pub_type: 杂志文章


    authors: Cheng Y,Qi Y,Zhu Q,Chen X,Wang N,Zhao X,Chen H,Cui X,Xu L,Zhang W

    更新日期:2009-06-01 00:00:00

  • Proteome characterization of sea star coelomocytes--the innate immune effector cells of echinoderms.

    abstract::Sea star coelomic fluid is in contact with all internal organs, carrying signaling molecules and a large population of circulating cells, the coelomocytes. These cells, also known as echinoderm blood cells, are responsible for the innate immune responses and are also known to have an important role in the first stage ...


    pub_type: 杂志文章


    authors: Franco CF,Santos R,Coelho AV

    更新日期:2011-09-01 00:00:00

  • In vitro oxidative footprinting provides insight into apolipoprotein B-100 structure in low-density lipoprotein.

    abstract::Low-density lipoprotein (LDL) is a major cholesterol carrier in human blood. Oxidations of apolipoprotein B-100 (apo B-100, LDL protein) could be proatherogenic and play critical roles in early stages of plaque formation in the arterial wall. The structure of apo B-100 is still poorly understood, partially due to its ...


    pub_type: 杂志文章


    authors: Chakraborty S,Cai Y,Tarr MA

    更新日期:2014-11-01 00:00:00

  • Prediction of the human membrane proteome.

    abstract::Membrane proteins are key molecules in the cell, and are important targets for pharmaceutical drugs. Few three-dimensional structures of membrane proteins have been obtained, which makes computational prediction of membrane proteins crucial for studies of these key molecules. Here, seven membrane protein topology pred...


    pub_type: 杂志文章


    authors: Fagerberg L,Jonasson K,von Heijne G,Uhlén M,Berglund L

    更新日期:2010-03-01 00:00:00

  • Popitam: towards new heuristic strategies to improve protein identification from tandem mass spectrometry data.

    abstract::In recent years, proteomics research has gained importance due to increasingly powerful techniques in protein purification, mass spectrometry and identification, and due to the development of extensive protein and DNA databases from various organisms. Nevertheless, current identification methods from spectrometric dat...


    pub_type: 杂志文章


    authors: Hernandez P,Gras R,Frey J,Appel RD

    更新日期:2003-06-01 00:00:00

  • Drug penetration and metabolism in 3D cell cultures treated in a 3D printed fluidic device: assessment of irinotecan via MALDI imaging mass spectrometry.

    abstract::Realistic in vitro models are critical in the drug development process. In this study, a novel in vitro platform is employed to assess drug penetration and metabolism. This platform, which utilizes a 3D printed fluidic device, allows for dynamic dosing of three dimensional cell cultures, also known as spheroids. The p...


    pub_type: 杂志文章


    authors: LaBonia GJ,Lockwood SY,Heller AA,Spence DM,Hummon AB

    更新日期:2016-06-01 00:00:00

  • Profiling of Histidine Phosphoproteome in Danio rerio by TiO2 Enrichment.

    abstract::Histidine phosphorylation is a reversible post-translational modification that is known to regulate signal transduction in prokaryotes. However, functional studies in eukaryotes have been largely neglected due to the labile nature of N-linked phosphorylated amino acids. In an effort to help elucidate the heretofore hi...


    pub_type: 杂志文章


    authors: Gao Y,Lee H,Kwon OK,Cheng Z,Tan M,Kim KT,Lee S

    更新日期:2019-05-01 00:00:00

  • Two-dimensional separation of human plasma proteins using iterative free-flow electrophoresis.

    abstract::Blood plasma is the most complex human-derived proteome, containing other tissue proteomes as subsets. This proteome has only been partially characterized due to the extremely wide dynamic range of the plasma proteins of more than ten orders of magnitude. Thus, the reduction in sample complexity prior to mass spectrom...


    pub_type: 杂志文章


    authors: Nissum M,Kuhfuss S,Hauptmann M,Obermaier C,Sukop U,Wildgruber R,Weber G,Eckerskorn C,Malmström J

    更新日期:2007-12-01 00:00:00

  • Minimally permutated peptide analogs as internal standards for relative and absolute quantification of peptides and proteins.

    abstract::A novel type of isobaric internal peptide standard for quantitative proteomics is described. The standard is a synthetic peptide derived from the target peptide by positional permutation of two amino acids. This type of internal standard is denominated minimally permutated peptide analog (MIPA). MIPA can be differenti...


    pub_type: 杂志文章


    authors: Winter D,Seidler J,Kugelstadt D,Derrer B,Kappes B,Lehmann WD

    更新日期:2010-04-01 00:00:00

  • Identification of ciliary neurotrophic factor receptor alpha as a mediator of neurotoxicity induced by alpha-synuclein.

    abstract::Accumulating evidence suggests that extracellular alpha-synuclein (eSNCA) plays an important role in the pathogenesis of Parkinson's disease or related synucleinopathies by inducing neurotoxicity directly or indirectly via microglial or astroglial activation. However, the mechanisms by which this occurs remain to be c...


    pub_type: 杂志文章


    authors: Liu J,Shi M,Hong Z,Zhang J,Bradner J,Quinn T,Beyer RP,Mcgeer PL,Chen S,Zhang J

    更新日期:2010-06-01 00:00:00

  • Biological versus technical variability in 2-D DIGE experiments with environmental bacteria.

    abstract::Two-dimensional difference gel electrophoresis (2-D DIGE) allows for reliable quantification of global protein abundance changes. The threshold of significance for protein abundance changes depends on the experimental variation (biological and technical). This study estimates biological, technical and total variation ...


    pub_type: 杂志文章


    authors: Zech H,Echtermeyer C,Wöhlbrand L,Blasius B,Rabus R

    更新日期:2011-08-01 00:00:00

  • Microparticles released from Mycobacterium tuberculosis-infected human macrophages contain increased levels of the type I interferon inducible proteins including ISG15.

    abstract::Microparticles (MPs) are small membranous particles (100-1000 nm) released under normal steady-state conditions and are thought to provide a communication network between host cells. Previous studies demonstrated that Mycobacterium tuberculosis (M. tb) infection of macrophages increased the release of MPs, and these M...


    pub_type: 杂志文章


    authors: Hare NJ,Chan B,Chan E,Kaufman KL,Britton WJ,Saunders BM

    更新日期:2015-09-01 00:00:00

  • Proteomic technologies for the identification of disease biomarkers in serum: advances and challenges ahead.

    abstract::Serum is an ideal biological sample that contains an archive of information due to the presence of a variety of proteins released by diseased tissue, and serum proteomics has gained considerable interest for the disease biomarker discovery. Easy accessibility and rapid protein changes in response to disease pathogenes...


    pub_type: 杂志文章,评审


    authors: Ray S,Reddy PJ,Jain R,Gollapalli K,Moiyadi A,Srivastava S

    更新日期:2011-06-01 00:00:00

  • Proteome analysis of Escherichia coli using high-performance liquid chromatography and Fourier transform ion cyclotron resonance mass spectrometry.

    abstract::The basic problem of complexity poses a significant challenge for proteomic studies. To date two-dimensional gel electrophoresis (2-DE) followed by enzymatic in-gel digestion of the peptides, and subsequent identification by mass spectrometry (MS) is the most commonly used method to analyze complex protein mixtures. H...


    pub_type: 杂志文章


    authors: Ihling C,Sinz A

    更新日期:2005-05-01 00:00:00

  • Determination of protein markers in human serum: Analysis of protein expression in toxic oil syndrome studies.

    abstract::Toxic oil syndrome (TOS) is a disease that appeared in Spain in 1981. It affected more than 20 000 people and produced over 300 deaths in the first 2 years. In this paper, a prospective study on the differences in gene expression in sera between a control versus a TOS-affected population, both originally exposed to th...


    pub_type: 杂志文章


    authors: Quero C,Colomé N,Prieto MR,Carrascal M,Posada M,Gelpí E,Abian J

    更新日期:2004-02-01 00:00:00

  • Proteomic analysis of Taenia solium metacestode excretion-secretion proteins.

    abstract::The metacestode larval stage of Taenia solium is the causal agent of a zoonotic disease called cysticercosis. The disease has an important impact on pork trade (due to porcine cysticercosis) and public health (due to human neurocysticercosis). In order to improve the current diagnostic tools and to get a better unders...


    pub_type: 杂志文章


    authors: Victor B,Kanobana K,Gabriël S,Polman K,Deckers N,Dorny P,Deelder AM,Palmblad M

    更新日期:2012-06-01 00:00:00

  • Proteomic analysis of the phytopathogenic fungus Botrytis cinerea during cellulose degradation.

    abstract::The ascomycete Botrytis cinerea is a phytopathogenic fungus infecting and causing significant yield losses in a number of crops. Moreover, in the last few years, B. cinerea has been adopted as an important model system in molecular phytopathology. In spite of these contributions, the molecular basis of the infection c...


    pub_type: 杂志文章


    authors: Fernández-Acero FJ,Colby T,Harzen A,Cantoral JM,Schmidt J

    更新日期:2009-05-01 00:00:00

  • Proteome analysis of chick embryonic cerebrospinal fluid.

    abstract::During early stages of embryo development, the brain cavity is filled with embryonic cerebrospinal fluid (E-CSF), a complex fluid containing different protein fractions that contributes to the regulation of the survival, proliferation and neurogenesis of the neuroectodermal stem cells. Using 2-DE, protein sequencing a...


    pub_type: 杂志文章


    authors: Parada C,Gato A,Aparicio M,Bueno D

    更新日期:2006-01-01 00:00:00

  • Sample preparation method for isolation of single-cell types from mouse liver for proteomic studies.

    abstract::It becomes increasingly clear that separation of pure cell populations provides a uniquely sensitive and accurate approach to protein profiling in biological systems and opens up a new area for proteomic analysis. The method we described could simultaneously isolate population of hepatocytes (HCs), hepatic stellate ce...


    pub_type: 杂志文章


    authors: Liu W,Hou Y,Chen H,Wei H,Lin W,Li J,Zhang M,He F,Jiang Y

    更新日期:2011-09-01 00:00:00

  • Albumin depletion of human plasma also removes low abundance proteins including the cytokines.

    abstract::The use of proteomics for efficient, accurate, and complete analysis of clinical samples poses a variety of technical challenges. The presence of higher abundance proteins in the plasma, such as albumin, may mask the detection of lower abundance proteins such as the cytokines. Methods have been proposed to deplete the...


    pub_type: 杂志文章


    authors: Granger J,Siddiqui J,Copeland S,Remick D

    更新日期:2005-12-01 00:00:00

  • Proteomics indicates modulation of tubulin polymerization by L-menthol inhibiting human epithelial colorectal adenocarcinoma cell proliferation.

    abstract::Menthol is a naturally occurring cyclic monoterpene used in oral hygiene products, confectionary, pharmaceuticals, cosmetics, pesticides, and as a flavoring agent. In the present study, we analyzed the differentially expressing proteome in L-menthol-treated Caco-2 cell line as it was found to inhibit cell proliferatio...


    pub_type: 杂志文章


    authors: Faridi U,Sisodia BS,Shukla AK,Shukla RK,Darokar MP,Dwivedi UN,Shasany AK

    更新日期:2011-05-01 00:00:00

  • High-sensitivity HLA class I peptidome analysis enables a precise definition of peptide motifs and the identification of peptides from cell lines and patients' sera.

    abstract::The characterization of peptides bound to human leukocyte antigen (HLA) class I is of fundamental importance for understanding CD8+ T cell-driven immunological processes and for the development of immunomodulatory therapeutic strategies. However, until now, the mass spectrometric analysis of HLA-bound peptides has typ...


    pub_type: 杂志文章


    authors: Ritz D,Gloger A,Weide B,Garbe C,Neri D,Fugmann T

    更新日期:2016-05-01 00:00:00

  • Proteome-wide analyses reveal diverse functions of acetylation proteins in Neurospora crassa.

    abstract::Quantitative acetyl-proteomics, a newly identified post-translational modification, is known to regulate transcriptional activity in different organisms. Neurospora crassa is a model ascomycete fungus maintained for biochemistry and molecular biology research; however, extensive studies of the functions of its acylati...


    pub_type: 杂志文章


    authors: Wang T,Guan W,Du Y,Xu Y,He Z,Zhang Y,Kang C,Wan X,Chi X,Sun K,Zhang X

    更新日期:2021-01-24 00:00:00

  • Plant-Microbe Symbiosis: What Has Proteomics Taught Us?

    abstract::Beneficial microbes have a positive impact on the productivity and fitness of the host plant. A better understanding of the biological impacts and underlying mechanisms by which the host derives these benefits will help to address concerns around global food production and security. The recent development of omics-bas...


    pub_type: 杂志文章,评审


    authors: Khatabi B,Gharechahi J,Ghaffari MR,Liu D,Haynes PA,McKay MJ,Mirzaei M,Salekdeh GH

    更新日期:2019-08-01 00:00:00

  • N-Terminal methylation of proteasome subunit Rpt1 in yeast.

    abstract::The 26S proteasome is a multicatalytic protease complex that degrades ubiquitinated proteins in eukaryotic cells. It consists of a proteolytic core (the 20S proteasome) as well as regulatory particles, which contain six ATPase (Rpt) subunits involved in unfolding and translocation of substrates to the catalytic chambe...


    pub_type: 杂志文章


    authors: Kimura Y,Kurata Y,Ishikawa A,Okayama A,Kamita M,Hirano H

    更新日期:2013-11-01 00:00:00