Pleiotropic molecular effects of the pro-apoptotic dietary constituent flavone in human colon cancer cells identified by protein and mRNA expression profiling.


:The flavonoid flavone contained in a variety of fruits and vegetables was identified as a very potent apoptosis inducer in human colonic cancer cells. In search of the molecular targets of flavone action in HT-29 cells we analyzed changes in mRNA and protein expression levels by proteomics and oligonucleotide array technologies. Proteome analysis identified several heat-shock proteins, annexins, and cytoskeletal caspase substrates as regulated by flavone and these protein classes are known to play a role in apoptosis induction and execution. Protein kinase C-beta, which serves as an ultimate marker for colon cancer development was no longer detectable in HT-29 cells exposed to flavone. Besides proteins involved in gene regulation or detoxification pathways, proteins involved in intermediary metabolism were altered by flavone exposure and this was associated with changes in the flux of energetic substrates. Oligonucleotide arrays, using chips with around 10 000 oligonucleotides spotted, revealed numerous changes in transcript levels of genes related to signaling, transcription, cancer development but also to metabolism. In conclusion, flavone has a surprisingly broad spectrum of effects on mRNA and protein expression in a human colonic cancer cell line with clusters of targets related to its apoptosis-inducing activity and to cellular metabolism.






Herzog A,Kindermann B,Döring F,Daniel H,Wenzel U




Has Abstract


2004-08-01 00:00:00












  • Alteration and localization of glycan-binding proteins in human hepatic stellate cells during liver fibrosis.

    abstract::Glycan-binding proteins (GBPs) play an important role in cell adhesion, bacterial/viral infection, and cellular signaling pathways. However, little is known about the precision alteration of GBPs referred to pathological changes in hepatic stellate cells (HSCs) during liver fibrosis. Here, the carbohydrate microarrays...


    pub_type: 杂志文章


    authors: Zhong Y,Qin Y,Dang L,Jia L,Zhang Z,Wu H,Cui J,Bian H,Li Z

    更新日期:2015-10-01 00:00:00

  • Screening the human serum proteome for genotype-phenotype associations: an analysis of the IL6 -174G>C polymorphism.

    abstract::Interleukin (IL)-6 is a circulatory, pleiotropic cytokine with multiple roles in the immune system. Both IL-6 and the IL6 -174G>C promoter polymorphism have been linked to various diseases associated with inflammation. However, the mechanism by which the polymorphism influences disease risk is unclear. We postulated t...


    pub_type: 杂志文章


    authors: Hegedus CM,Skibola CF,Bracci P,Holly EA,Smith MT

    更新日期:2007-02-01 00:00:00

  • Staphylococcus aureus ClpC ATPase is a late growth phase effector of metabolism and persistence.

    abstract::Staphylococcus aureus Clp ATPases (molecular chaperones) alter normal physiological functions including an aconitase-mediated effect on post-stationary growth, acetate catabolism, and entry into death phase (Chatterjee et al., J. Bacteriol. 2005, 187, 4488-4496). In the present study, the global function of ClpC in ph...


    pub_type: 杂志文章


    authors: Chatterjee I,Schmitt S,Batzilla CF,Engelmann S,Keller A,Ring MW,Kautenburger R,Ziebuhr W,Hecker M,Preissner KT,Bischoff M,Proctor RA,Beck HP,Lenhof HP,Somerville GA,Herrmann M

    更新日期:2009-03-01 00:00:00

  • Proteomics analysis in Caenorhabditis elegans to elucidate the response induced by tyrosol, an olive phenol that stimulates longevity and stress resistance.

    abstract::Tyrosol (TYR, 2-(4-hydroxyphenyl)ethanol), one of the main phenols in olive oil and olive fruit, significantly strengthens resistance to thermal and oxidative stress in the nematode Caenorhabditis elegans and extends its lifespan. To elucidate the cellular functions regulated by TYR, we have used a proteomic procedure...


    pub_type: 杂志文章


    authors: Cañuelo A,Peragón J

    更新日期:2013-10-01 00:00:00

  • Human plasma proteome analysis by reversed sequence database search and molecular weight correlation based on a bacterial proteome analysis.

    abstract::In shotgun proteomics, proteins can be fractionated by 1-D gel electrophoresis and digested into peptides, followed by liquid chromatography to separate the peptide mixture. Mass spectrometry generates hundreds of thousands of tandem mass spectra from these fractions, and proteins are identified by database searching....


    pub_type: 杂志文章


    authors: Park GW,Kwon KH,Kim JY,Lee JH,Yun SH,Kim SI,Park YM,Cho SY,Paik YK,Yoo JS

    更新日期:2006-02-01 00:00:00

  • Isobaric Tag-Based Protein Profiling across Eight Human Cell Lines Using High-Field Asymmetric Ion Mobility Spectrometry and Real-Time Database Searching.

    abstract::A vast number of human cell lines are available for cell culture model-based studies, and as such the potential exists for discrepancies in findings due to cell line selection. To investigate this concept, the authors determine the relative protein abundance profiles of a panel of eight diverse, but commonly studied h...


    pub_type: 杂志文章


    authors: Liu X,Gygi SP,Paulo JA

    更新日期:2021-01-01 00:00:00

  • Selective enrichment of Ser-/Thr-phosphorylated peptides in the presence of Ser-/Thr-glycosylated peptides.

    abstract::Modification through beta-elimination has proven to be a reliable first step in the approach for enrichment of serine/threonine-phopshorylated (Ser-/Thr) peptides. However, under harsh basic conditions, Ser-/Thr-glycosylated peptides are susceptible to beta-elimination as well. Therefore, we have optimized these condi...


    pub_type: 杂志文章


    authors: Poot AJ,Ruijter E,Nuijens T,Dirksen EH,Heck AJ,Slijper M,Rijkers DT,Liskamp RM

    更新日期:2006-12-01 00:00:00

  • Proteomic profiling for cancer progression: Differential display analysis for the expression of intracellular proteins between regressive and progressive cancer cell lines.

    abstract::Tumor development and progression consist of a series of complex processes involving multiple changes in gene expression (Paolo et al. Physiol. Rev., 1993, 73, 161-195; Lance et al. Cell., 1991, 64, 327-336). Tumor cells acquire an invasive and metastatic phenotype that is the main cause of death for cancer patients. ...


    pub_type: 杂志文章


    authors: Hayashi E,Kuramitsu Y,Okada F,Fujimoto M,Zhang X,Kobayashi M,Iizuka N,Ueyama Y,Nakamura K

    更新日期:2005-03-01 00:00:00

  • A simple and rapid protein array based method for the simultaneous detection of biowarfare agents.

    abstract::A protein chip has been developed that allows the simultaneous detection of a multitude of different biowarfare agents. The chip was developed for the ArrayTube platform providing a cheap and easy to handle technology solution that combines a microtube-integrated protein chip with the classical procedure of a sandwich...


    pub_type: 杂志文章


    authors: Huelseweh B,Ehricht R,Marschall HJ

    更新日期:2006-05-01 00:00:00

  • Insights from Proteomic Studies into Plant Somatic Embryogenesis.

    abstract::Somatic embryogenesis is a biotechnological approach mainly used for the clonal propagation of different plants worldwide. In somatic embryogenesis, embryos arise from somatic cells under appropriate culture conditions. This plasticity in plants is a demonstration of true cellular totipotency and is the best approach ...


    pub_type: 杂志文章,评审


    authors: Heringer AS,Santa-Catarina C,Silveira V

    更新日期:2018-03-01 00:00:00

  • The application of proteomics in defining the T cell antigens of Mycobacterium tuberculosis.

    abstract::The complete sequencing of the Mycobacterium tuberculosis genome offers a unique opportunity to fully elucidate the biology of this human pathogen. One aspect of significant importance is the definition of T cell antigens. This report describes the development and implementation of a proteomic approach to defining suc...


    pub_type: 杂志文章


    authors: Covert BA,Spencer JS,Orme IM,Belisle JT

    更新日期:2001-04-01 00:00:00

  • Investigating the macropinocytic proteome of Dictyostelium amoebae by high-resolution mass spectrometry.

    abstract::The cellular slime mold Dictyostelium discoideum is a soil-living eukaryote, which feeds on microorganisms engulfed by phagocytosis. Axenic laboratory strains have been produced that are able to use liquid growth medium internalized by macropinocytosis as the source of food. To better define the macropinocytosis proce...


    pub_type: 杂志文章


    authors: Journet A,Klein G,Brugière S,Vandenbrouck Y,Chapel A,Kieffer S,Bruley C,Masselon C,Aubry L

    更新日期:2012-01-01 00:00:00

  • Advancing the sensitivity of selected reaction monitoring-based targeted quantitative proteomics.

    abstract::Selected reaction monitoring (SRM) - also known as multiple reaction monitoring (MRM) - has emerged as a promising high-throughput targeted protein quantification technology for candidate biomarker verification and systems biology applications. A major bottleneck for current SRM technology, however, is insufficient se...


    pub_type: 杂志文章,评审


    authors: Shi T,Su D,Liu T,Tang K,Camp DG 2nd,Qian WJ,Smith RD

    更新日期:2012-04-01 00:00:00

  • Proteomic analysis in human breast cancer: identification of a characteristic protein expression profile of malignant breast epithelium.

    abstract::Gene expression analysis has become a promising tool in predicting the clinical course of malignant disease and the response to antineoplastic therapy. Surprisingly, only little is known about the protein expression pattern of human tumors. Recent advances in proteomic analysis allow proteins of interest to be identif...


    pub_type: 杂志文章


    authors: Hudelist G,Singer CF,Pischinger KI,Kaserer K,Manavi M,Kubista E,Czerwenka KF

    更新日期:2006-03-01 00:00:00

  • Proteome analysis of chick embryonic cerebrospinal fluid.

    abstract::During early stages of embryo development, the brain cavity is filled with embryonic cerebrospinal fluid (E-CSF), a complex fluid containing different protein fractions that contributes to the regulation of the survival, proliferation and neurogenesis of the neuroectodermal stem cells. Using 2-DE, protein sequencing a...


    pub_type: 杂志文章


    authors: Parada C,Gato A,Aparicio M,Bueno D

    更新日期:2006-01-01 00:00:00

  • An improved mechanically durable electrophoresis gel matrix that is fully compatible with fluorescence-based protein detection technologies.

    abstract::Unfortunately, conventional large-format polyacrylamide gels are mechanically fragile, often tearing during the subsequent manipulations required for visualization of the proteins. This problem is compounded when large-format two-dimensional gels are subjected to multiple staining procedures in order to detect differe...


    pub_type: 杂志文章


    authors: Schulenberg B,Arnold B,Patton WF

    更新日期:2003-07-01 00:00:00

  • Holistic differential analysis of embryo-induced alterations in the proteome of bovine endometrium in the preattachment period.

    abstract::During the peri-implantation period, molecular signaling between embryo and endometrium (layer of tissue lining the uterus lumen) is supposed to be crucial for the maintenance of pregnancy. To investigate embryo-induced alterations in the proteome of bovine endometrium in the preattachment period (day 18), we used mon...


    pub_type: 杂志文章


    authors: Berendt FJ,Fröhlich T,Schmidt SE,Reichenbach HD,Wolf E,Arnold GJ

    更新日期:2005-07-01 00:00:00

  • Highly Efficient Identification of O-GalNAc Glycosylation by an Acid-Assisted Glycoform Simplification Approach.

    abstract::Compared with N-linked glycosylation, the analysis of O-GalNAc glycosylation is extremely challenging due to the high structure diversity of glycans and lack of glycosidases to release O-GalNAc glycans. In this work, a glycoform simplification strategy by combining HILIC enrichment with chemical de-sialylation to char...


    pub_type: 杂志文章


    authors: You X,Qin H,Mao J,Tian Y,Dong M,Guo Z,Liang X,Wang L,Jin Y,Ye M

    更新日期:2018-09-01 00:00:00

  • Evaluation and optimization of IgY spin column technology in the depletion of abundant proteins from human serum.

    abstract::Serum depletion strategies are commonly implemented in order to remove abundant proteins, increasing the number of proteins detected in a biomarker study. The IgY spin columns used in this study bind 12 and 14 primate proteins, respectively. 1-D SDS-PAGE and 2-DE revealed a suboptimal performance of the IgY spin colum...


    pub_type: 杂志文章


    authors: Corrigan L,Jefferies C,Clive Lee T,Daly J

    更新日期:2011-08-01 00:00:00

  • 2-D DIGE analysis of the mitochondrial proteome from human skeletal muscle reveals time course-dependent remodelling in response to 14 consecutive days of endurance exercise training.

    abstract::Adaptation of skeletal muscle to repeated bouts of endurance exercise increases aerobic capacity and improves mitochondrial function. However, the adaptation of human skeletal muscle mitochondrial proteome to short-term endurance exercise training has not been investigated. Eight sedentary males cycled for 60 min at 8...


    pub_type: 杂志文章


    authors: Egan B,Dowling P,O'Connor PL,Henry M,Meleady P,Zierath JR,O'Gorman DJ

    更新日期:2011-04-01 00:00:00

  • Quantitative proteomics of heavy metal stress responses in Sydney rock oysters.

    abstract::Currently, there are few predictive biomarkers in key biomonitoring species, such as oysters, that can detect heavy metal pollution in coastal waterways. Several attributes make oysters superior to other organisms for positive biomonitoring of heavy metal pollution. In particular, they are filter feeders with a high c...


    pub_type: 杂志文章


    authors: Muralidharan S,Thompson E,Raftos D,Birch G,Haynes PA

    更新日期:2012-03-01 00:00:00

  • Improving the quality of genome, protein sequence, and taxonomy databases: a prerequisite for microbiome meta-omics 2.0.

    abstract::High-throughput shotgun metaproteomic approaches on environmental or medical microbiomes are producing huge amounts of tandem mass spectrometry data. These can be interpreted either with a general protein sequence database comprising tens of thousands of sequenced genomes or with a more customized database such as tho...


    pub_type: 杂志文章


    authors: Pible O,Armengaud J

    更新日期:2015-10-01 00:00:00

  • Enabling BioSharing - a report on the Annual Spring Workshop of the HUPO-PSI April 11-13, 2011, EMBL-Heidelberg, Germany.

    abstract::The Annual Spring workshop of the HUPO-PSI was this year held at the EMBL International Centre for Advanced Training (EICAT) in Heidelberg, Germany. Delegates briefly reviewed the successes of the group to date. These include the wide spread implementation of the molecular interaction data exchange formats, PSI-MI XML...




    authors: Orchard S,Albar JP,Deutsch EW,Eisenacher M,Vizcaíno JA,Hermjakob H

    更新日期:2011-11-01 00:00:00

  • NDRG1 is down-regulated in the early apoptotic event induced by camptothecin analogs: the potential role in proteolytic activation of PKC delta and apoptosis.

    abstract::We previously reported that NSC606985, a new camptothecin analog, induces apoptosis of acute myeloid leukemic cells, which is triggered by proteolytic activation of protein kinase C delta (PKC delta). Here, we performed quantitative proteomic analysis of NSC606985-treated and untreated leukemic U937 cells with two-dim...


    pub_type: 杂志文章


    authors: Zheng Y,Wang LS,Xia L,Han YH,Liao SH,Wang XL,Cheng JK,Chen GQ

    更新日期:2009-04-01 00:00:00

  • 6th HUPO Annual World Congress - Proteomics Standards Initiative Workshop 6-10 October 2007, Seoul, Korea.

    abstract::The plenary session of the Proteomics Standards Initiative of the Human Proteome Organisation at the 6th annual HUPO world congress updated the delegates on the current status of the ongoing work of this group. The new MS interchange format, mzML, had been entered into the HUPO document process immediately prior to th...




    authors: Orchard S,Martens L,Tasman J,Binz PA,Albar JP,Hermjakob H

    更新日期:2008-04-01 00:00:00

  • Characterization of heat shock protein 27 phosphorylation sites in renal cell carcinoma.

    abstract::The phosphorylation of heat shock protein 27 (HSP27) occurs differently in human renal cell carcinoma (RCC) compared to homologous normal kidney tissue. Two-dimensional electrophoresis was used to separate and visualize HSP27, via immunostaining with anti-HSP27 antibody, in tumor and normal renal samples, obtained aft...


    pub_type: 杂志文章


    authors: Tremolada L,Magni F,Valsecchi C,Sarto C,Mocarelli P,Perego R,Cordani N,Favini P,Galli Kienle M,Sanchez JC,Hochstrasser DF,Corthals GL

    更新日期:2005-02-01 00:00:00

  • Identification of Arabidopsis salt and osmotic stress responsive proteins using two-dimensional difference gel electrophoresis and mass spectrometry.

    abstract::Arabidopsis thaliana cell suspension cultures have been used to investigate the effects of salinity and hyperosmotic stress on plant cellular proteins. We show that 200 mM NaCl and 400 mM sorbitol treatments induce extracellular medium acidification in Arabidopsis cell cultures, a typical response of plant cells to sa...


    pub_type: 杂志文章


    authors: Ndimba BK,Chivasa S,Simon WJ,Slabas AR

    更新日期:2005-11-01 00:00:00

  • Tyrosine 656 in topoisomerase IIβ is important for the catalytic activity of the enzyme: Identification based on artifactual +80-Da modification at this site.

    abstract::Topoisomerase (topo) II catalyzes topological changes in DNA. Although both human isozymes, topo IIα and β are phosphorylated, site-specific phosphorylation of topo IIβ is poorly characterized. Using LC-MS/MS analysis of topo IIβ, cleaved with trypsin, Arg C or cyanogen bromide (CNBr) plus trypsin, we detected four +8...


    pub_type: 杂志文章


    authors: Grozav AG,Willard BB,Kozuki T,Chikamori K,Micluta MA,Petrescu AJ,Kinter M,Ganapathi R,Ganapathi MK

    更新日期:2011-03-01 00:00:00

  • Proteomic analysis of the anterior cingulate cortex in the major psychiatric disorders: Evidence for disease-associated changes.

    abstract::Abnormalities of the anterior cingulate cortex have previously been described in schizophrenia, major depressive disorder and bipolar disorder. In this study 2-DE was performed followed by mass spectrometric sequencing to identify disease-specific protein changes within the anterior cingulate cortex in these psychiatr...


    pub_type: 杂志文章


    authors: Beasley CL,Pennington K,Behan A,Wait R,Dunn MJ,Cotter D

    更新日期:2006-06-01 00:00:00

  • "You produce while I clean up", a strategy revealed by exoproteomics during Synechococcus-Roseobacter interactions.

    abstract::Most of the energy that is introduced into the oceans by photosynthetic primary producers is in the form of organic matter that then sustains the rest of the food web, from micro to macro-organisms. However, it is the interactions between phototrophs and heterotrophs that are vital to maintaining the nutrient balance ...


    pub_type: 杂志文章


    authors: Christie-Oleza JA,Scanlan DJ,Armengaud J

    更新日期:2015-10-01 00:00:00