Abstract:
:The current study used three different proteomic strategies, which differed by their extent of intact protein separation, to examine the proteome of a pluripotent mouse embryonic stem cell line, R1. Proteins from whole-cell lysates were subjected either to 2-D-LC, or 1-DE, or were unfractionated prior to enzymatic digestion and subsequent analysis by MS. The results yielded 1895 identified non-redundant proteins and, for 128 of these, the specific isoform could be determined based on detection of an isoform-specific peptide. When compared with two previously published proteomic studies that used the same cell line, the current study reveals 612 new proteins.
journal_name
Proteomicsjournal_title
Proteomicsauthors
Gundry RL,Tchernyshyov I,Sheng S,Tarasova Y,Raginski K,Boheler KR,Van Eyk JEdoi
10.1002/pmic.201000039subject
Has Abstractpub_date
2010-07-01 00:00:00pages
2728-32issue
14eissn
1615-9853issn
1615-9861journal_volume
10pub_type
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