Abstract:
:Absolute protein concentration determination is becoming increasingly important in a number of fields including diagnostics, biomarker discovery and systems biology modeling. The recently introduced quantification concatamer methodology provides a novel approach to performing such determinations, and it has been applied to both microbial and mammalian systems. While a number of software tools exist for performing analyses of quantitative data generated by related methodologies such as SILAC, there is currently no analysis package dedicated to the quantification concatamer approach. Furthermore, most tools that are currently available in the field of quantitative proteomics do not manage storage and dissemination of such data sets.
journal_name
Proteomicsjournal_title
Proteomicsauthors
Swainston N,Jameson D,Carroll Kdoi
10.1002/pmic.201000454subject
Has Abstractpub_date
2011-01-01 00:00:00pages
329-33issue
2eissn
1615-9853issn
1615-9861journal_volume
11pub_type
杂志文章相关文献
PROTEOMICS文献大全abstract::Transcription factors (TFs) are a family of DNA-binding proteins whose gene regulatory capabilities are of vital importance in defining the molecular state of a cell. Despite their biological significance, our understanding of TF behavior and function is still limited. This is because we have so far mostly relied on g...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.201600317
更新日期:2017-02-01 00:00:00
abstract::During clinical mastitis in dairy cows, the quantity of milk produced decreases and the composition of the milk is altered. As the severity of inflammation associated with the disease increases, the chemical composition of milk approaches that of blood as a consequence of increased permeability of the blood mammary ba...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200300723
更新日期:2004-07-01 00:00:00
abstract::Hepatocellular carcinoma (HCC) is the second cause of cancer-related deaths worldwide. A clearer understanding of the molecular mechanisms underlying tumor growth and invasiveness remains crucial for developing new therapies. Here, the expression of tetraspanins, a family of plasma membrane organizers involved in tumo...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201900025
更新日期:2019-11-01 00:00:00
abstract::Previous studies have found that some first onset schizophrenia patients show signs of impaired insulin signaling. Also, epidemiological studies have shown that periods of suboptimal nutrition including protein deficiencies during pregnancy can lead to increased incidence of metabolic conditions and psychiatric disord...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201200376
更新日期:2012-12-01 00:00:00
abstract::DIGE is a protein labelling and separation technique allowing quantitative proteomics of two or more samples by optical fluorescence detection of differentially labelled proteins that are electrophoretically separated on the same gel. DIGE is an alternative to quantitation by MS-based methodologies and can circumvent ...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.200800298
更新日期:2008-12-01 00:00:00
abstract::The E7 protein produced by high-risk human papillomavirus (HPV) induces a degradation of the retinoblastoma tumor suppressor RB through direct interaction, which suggests that an inhibitor for the interaction can be a potential anticancer drug. A surface plasmon resonance (SPR) imaging-based protein array chip was dev...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200500001
更新日期:2005-11-01 00:00:00
abstract::The allergen content of standardized pollen material is crucial for an effective diagnosis and treatment. However, variations in IgE reactivities of allergic patients to different preparations of Phleum pratense pollen have been reported. In order to define and directly compare the allergen composition of pollen prepa...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201000451
更新日期:2010-12-01 00:00:00
abstract::MS protein identification and quantitation are key proteomic techniques in biological research. Besides identification of proteins, MS is used increasingly to characterize secondary protein modifications. This often requires trimming the analytical strategy to a specific type of modification. Direct analysis of protei...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200501332
更新日期:2006-01-01 00:00:00
abstract::By means of a degradomic approach applying proteomic techniques, we previously suggested that apolipoprotein E (apoE) is a substrate of matrix metalloproteinase-14 (MMP-14). Here we confirm that apoE is, in fact, a substrate of MMP-14 and also of MMP-7 and MMP-2 to a lesser extent. The 34 kDa apoE protein was initiall...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200700487
更新日期:2008-07-01 00:00:00
abstract::Human butyrophilin (BTN) expression in milk fat globule (MFGM) was evaluated using two dimensional electrophoresis (2-DE) as the separation technique, and peptide mass mapping by matrix-assisted laser desorption/ionisation-mass spectrometry (MALDI-MS) as the identification tool. Since milk composition changes througho...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/1615-9861(200207)2:7<850::AID-PROT850>3.0.
更新日期:2002-07-01 00:00:00
abstract::Radiation therapy (RT), combined with chemotherapy, is currently the standard adjuvant approach for UICC-stage II and III rectal cancer patients. Individual rectal tumors display wide ranges of radiosensitivity (RS). The aim of the present study was to identify proteins associated with radioresistance (RR), with the f...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200300854
更新日期:2004-08-01 00:00:00
abstract::Many types of cells transit in vitro from a two- to a three-dimensional growth, when they are exposed to microgravity. The underlying mechanisms are not yet understood. Hence, we investigated the impact of microgravity on protein content and growth behavior. For this purpose, the human thyroid cancer cells FTC-133 wer...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201500067
更新日期:2015-09-01 00:00:00
abstract::The quantification of changes in protein abundance in complex biological specimens is essential for proteomic studies in basic and applied research. Here we report on the development and validation of the DeepQuanTR software for identification and quantification of differentially expressed proteins using LC-MALDI-MS. ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200900634
更新日期:2010-07-01 00:00:00
abstract::Human plasma is the most clinically valuable specimen, containing not only a dynamic concentration range of protein components, but also several groups of high-abundance proteins that seriously interfere with the detection of low-abundance potential biomarker proteins. To establish a high-throughput method for efficie...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200401310
更新日期:2005-08-01 00:00:00
abstract::The pilot phase of the Human Brain Proteome Project as a part of the Human Proteome Organisation has just been started. In two pilot studies, 18 different laboratories are analyzing mouse brains of three age stages and human brain autopsy versus biopsy material, respectively. The overall aim is to elucidate the portfo...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200400867
更新日期:2004-08-01 00:00:00
abstract::The community working on model organisms is growing steadily and the number of model organisms for which proteome data are being generated is continuously increasing. To standardize efforts and to make optimal use of proteomics data acquired from model organisms, a new Human Proteome Organisation (HUPO) initiative on ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201290014
更新日期:2012-02-01 00:00:00
abstract::PROTICdb is a web-based application, mainly designed to store and analyze plant proteome data obtained by two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) and mass spectrometry (MS). The purposes of PROTICdb are (i) to store, track, and query information related to proteomic experiments, i.e., from tissue...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200401111
更新日期:2005-05-01 00:00:00
abstract::The useful structural features of class II MHC molecules are rarely integrated into T-cell epitope predictions. We propose an approach that applies a novel expectation-maximization algorithm to align the naturally processed peptides selected by the class II MHC I-A(g7) molecule - focusing on the five MHC-specific anch...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200600584
更新日期:2007-02-01 00:00:00
abstract::Collagen-related peptide (CRP) stimulates powerful activation of platelets through the glycoprotein VI (GPVI)-FcR gamma-chain complex. We have combined proteomics and traditional biochemistry approaches to study the proteome of CRP-activated platelets, focusing in detail on tyrosine phosphorylation. In two separate ap...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200600299
更新日期:2006-10-01 00:00:00
abstract::The proteomic profile of hypothalamus, a key organ of CNS, is explored here by employing two widely used MS techniques, i.e. HPLC/ESI-ion trap and HPLC/ESI-quadrupole-TOF MS. Strong cation exchange is used for the fractionation of peptides and protein search engine MASCOT is employed for data query. One hundred and th...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201300073
更新日期:2013-08-01 00:00:00
abstract::The characterization of peptides bound to human leukocyte antigen (HLA) class I is of fundamental importance for understanding CD8+ T cell-driven immunological processes and for the development of immunomodulatory therapeutic strategies. However, until now, the mass spectrometric analysis of HLA-bound peptides has typ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201500445
更新日期:2016-05-01 00:00:00
abstract::We performed phosphoproteome analysis of proteins from the extremely thermophilic Gram-negative eubacterium Thermus thermophilus HB8 using gel-free mass spectrometric method. We identified 52 phosphopeptides from 48 proteins and determined 46 phosphorylation sites: 30 on serine, 12 on threonine, and 4 on tyrosine. The...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201100573
更新日期:2012-05-01 00:00:00
abstract::Leukaemic transformation is frequently associated with the aberrant activity of a protein tyrosine kinase (PTK). As such it is of clinical relevance to be able to map the effects of these leukaemogenic PTKs on haemopoietic cells at the level of phosphorylation modulation. In this paradigm study we have employed a rang...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201000176
更新日期:2010-09-01 00:00:00
abstract::Chemokines (CCs) are small chemoattractant cytokines involved in a wide variety of biological and pathological processes. Released by cells in the milieu, and extracellular matrix and activating signalling cascades upon binding to specific G protein-coupled receptors (GPCRs), they trigger many cellular events. In vari...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200800211
更新日期:2008-11-01 00:00:00
abstract::Arabidopsis thaliana cell suspension cultures have been used to investigate the effects of salinity and hyperosmotic stress on plant cellular proteins. We show that 200 mM NaCl and 400 mM sorbitol treatments induce extracellular medium acidification in Arabidopsis cell cultures, a typical response of plant cells to sa...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200401282
更新日期:2005-11-01 00:00:00
abstract::Low levels and long term exposure to benzene is associated with hematotoxicity including aplastic anemia, acute myelogenous leukemia, and lymphoma. Current biomonitoring methods such as urinary phenol, S-phenylmercapturic acid, and trans-trans muconic acid were found to be unreliable as analytical methods to detect be...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200300616
更新日期:2003-12-01 00:00:00
abstract::Trichomonas vaginalis, a sexually transmitted parasite, has many cysteine proteinases (CPs); some are involved in trichomonal pathogenesis, express during infection, and antibodies against CPs have been detected in patient sera. The goal of this study was to identify the antigenic proteinases of T. vaginalis as potent...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200900479
更新日期:2010-02-01 00:00:00
abstract::The authors present pathwayPCA, an R/Bioconductor package for integrative pathway analysis that utilizes modern statistical methodology, including supervised and adaptive, elastic-net, sparse principal component analysis. pathwayPCA can be applied to continuous, binary, and survival outcomes in studies with multiple c...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201900409
更新日期:2020-11-01 00:00:00
abstract::The complete sequencing of the Mycobacterium tuberculosis genome offers a unique opportunity to fully elucidate the biology of this human pathogen. One aspect of significant importance is the definition of T cell antigens. This report describes the development and implementation of a proteomic approach to defining suc...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/1615-9861(200104)1:4<574::AID-PROT574>3.0.
更新日期:2001-04-01 00:00:00
abstract::Antibodies that recognize PTMs of histones play a central role in epigenetic proteomic research. Modification-specific antibodies are employed in chromatin immunoprecipitation, for Western blotting and during the immunoprecipitation steps for MS-based global proteomic analyses. Knowledge about the antibodies' off-targ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201200383
更新日期:2013-03-01 00:00:00