Abstract:
:Gan et al. (Proteomics 2013, 13, 3117-3123) described a new "macropore" protocol for effective protein digestion by trypsin suitable for a wide range of pH including acidic pH. It was effective not only in experiments with solutions of a model protein (myoglobin), but also with a subfraction of rat liver cytosol. This significantly simplifies and accelerates protein digestion procedures for subsequent MS. However, further studies are needed to find limits of experimental applicability of the described protocol in proteomics.
journal_name
Proteomicsjournal_title
Proteomicsauthors
Medvedev AEdoi
10.1002/pmic.201300440subject
Has Abstractpub_date
2013-11-01 00:00:00pages
3101-2issue
21eissn
1615-9853issn
1615-9861journal_volume
13pub_type
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