Abstract:
:The use of proteomics for efficient, accurate, and complete analysis of clinical samples poses a variety of technical challenges. The presence of higher abundance proteins in the plasma, such as albumin, may mask the detection of lower abundance proteins such as the cytokines. Methods have been proposed to deplete the sample of these higher abundance proteins to facilitate detection of those with lower abundance. In this study, a commercially available albumin depletion kit was used to determine if removal of albumin would measurably reduce detection of lower abundance cytokine proteins in human plasma. The Montage Albumin Deplete Kit (Millipore) was used to deplete albumin from LPS-stimulated whole blood from 15 normal human donors. Albumin depletion was measured using the BCG reagent and SDS-PAGE, and cytokine recovery was determined by a microassay immunoassay that measures both pro- and anti-inflammatory cytokines. Average albumin depletion from the samples was 72%. However, several cytokines were also significantly reduced when the albumin was removed from the plasma. Additionally, there was a variable reduction in cytokine recovery from a known mixture of cytokines in a minimal amount of plasma that were loaded onto the columns. These data demonstrate that there may be a non-specific loss of cytokines following albumin depletion, which may confound subsequent proteomic analysis.
journal_name
Proteomicsjournal_title
Proteomicsauthors
Granger J,Siddiqui J,Copeland S,Remick Ddoi
10.1002/pmic.200401331subject
Has Abstractpub_date
2005-12-01 00:00:00pages
4713-8issue
18eissn
1615-9853issn
1615-9861journal_volume
5pub_type
杂志文章相关文献
PROTEOMICS文献大全abstract::Elite rice hybrids, when compared to their parental lines, exhibit increased yield and other favorable agronomical traits, such as pathogen- and water-stress resistances, which are described as heterosis, and the molecular mechanism of heterosis remains to be elucidated. Since genomic sequences of the paternal (9311) ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200701164
更新日期:2008-11-01 00:00:00
abstract::White spot syndrome virus (WSSV) is a pathogen that causes considerable mortality of the farmed shrimp, Penaeus monodon. Candidate 'vaccines', WSSV envelope protein VP28 and formalin-inactivated WSSV, can provide short-lived protection against the virus. In this study, P. monodon was orally intubated with the aforemen...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201300405
更新日期:2014-07-01 00:00:00
abstract::When the p-value is set at <0.05 in statistical group comparisons, a 5% rate of "false significant" results is expected. In order to test the reliability of our 2-DE method, we loaded each of 24 gels with equal-sized samples (200 mug protein from pooled rat brain, pH 4-7, stained with ruthenium fluorescent stain for v...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200701074
更新日期:2008-05-01 00:00:00
abstract::Several lines of evidence suggest that hnRNPs A/B (hnRNPs A1, A2/B1, and A3) play an important role in proliferation, although the functional overlap among members of hnRNPs A/B remains largely unknown. In this study, we have employed RNAi knockdown and proteomic approaches to investigate the biological functions of h...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201300549
更新日期:2014-06-01 00:00:00
abstract::Employing classical two-dimensional electrophoresis (2-DE), amino acid sequencing and immunoblot analysis, we examine for the first time the effect of ozone, a highly notorious environmental pollutant, on rice seedling proteins. Drastic visible necrotic damage to leaf by ozone and consequent increase in ascorbate pero...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/1615-9861(200208)2:8<947::AID-PROT947>3.0.
更新日期:2002-08-01 00:00:00
abstract::Infection with the human liver fluke, Opisthorchis viverrini, is a serious public health problem in Thailand, Laos and nearby locations in Southeast Asia. Both experimental and epidemiological evidence strongly implicate liver fluke infection in the etiology of one of the liver cancer subtypes, cholangiocarcinoma (CCA...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200900393
更新日期:2010-03-01 00:00:00
abstract::The use of isobaric tags such as iTRAQ allows the relative and absolute quantification of hundreds of proteins in a single experiment for up to eight different samples. More classical techniques such as 2-DE can offer a complimentary approach for the analysis of complex protein samples. In this study, the proteomes of...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200900836
更新日期:2010-05-01 00:00:00
abstract::New insights on cellular heterogeneity in the last decade provoke the development of a variety of single cell omics tools at a lightning pace. The resultant high-dimensional single cell data generated by these tools require new theoretical approaches and analytical algorithms for effective visualization and interpreta...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.201600267
更新日期:2017-02-01 00:00:00
abstract::By mining publicly accessible databases, we have developed a collection of tissue-specific predictive protein expression maps as a function of cancer histological state. Data analysis is applied to the differential expression of gene products in pooled libraries from the normal to the altered state(s). We wish to repo...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200300488
更新日期:2003-08-01 00:00:00
abstract::In this work, we first immobilized tin(IV) ion on polydopamine-coated magnetic graphene (magG@PDA) to synthesize Sn4+ -immobilized magG@PDA (magG@PDA-Sn4+ ) and successfully applied the material to highly selective enrichment of phosphopeptides. The material gathered the advantages of large surface area of graphene, s...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201600187
更新日期:2016-11-01 00:00:00
abstract::This is the first broad proteomic description of Gluconacetobacter diazotrophicus, an endophytic bacterium, responsible for the major fraction of the atmospheric nitrogen fixed in sugarcane in tropical regions. Proteomic coverage of G. diazotrophicus PAL5 was obtained by two independent approaches: 2-DE followed by MA...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200700912
更新日期:2008-04-01 00:00:00
abstract::The field of biochemistry is currently faced with the enormous challenge of assigning functional significance to more than thirty thousand predicted protein products encoded by the human genome. In order to accomplish this daunting task, methods will be required that facilitate the global analysis of proteins in compl...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/1615-9861(200109)1:9<1067::AID-PROT1067>3.
更新日期:2001-09-01 00:00:00
abstract::The allergen content of standardized pollen material is crucial for an effective diagnosis and treatment. However, variations in IgE reactivities of allergic patients to different preparations of Phleum pratense pollen have been reported. In order to define and directly compare the allergen composition of pollen prepa...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201000451
更新日期:2010-12-01 00:00:00
abstract::Eukaryotic lipid rafts are membrane microdomains that have significant amounts of cholesterol and a selective set of proteins that have been associated with multiple biological functions. The Lyme disease agent, Borrelia burgdorferi, is one of an increasing number of bacterial pathogens that incorporates cholesterol o...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201500093
更新日期:2015-11-01 00:00:00
abstract::The recent development in immune checkpoint inhibitors and chimeric antigen receptor (CAR) T-cells in the treatment of cancer has not only demonstrated the potency of utilizing T-cell reactivity for cancer therapy, but has also highlighted the need for developing new approaches to discover targets suitable for such no...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201700465
更新日期:2018-06-01 00:00:00
abstract::The 2-D peptide separations employing mixed mode reversed phase anion exchange (MM (RP-AX)) HPLC in the first dimension in conjunction with RP chromatography in the second dimension were developed and utilised for shotgun proteome analysis. Compared with strong cation exchange (SCX) typically employed for shotgun prot...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200900669
更新日期:2010-08-01 00:00:00
abstract::A monolithic enzymatic microreactor was prepared in a fused-silica capillary by in situ polymerization of acrylamide, glycidyl methacrylate (GMA) and ethylene dimethacrylate (EDMA) in the presence of a binary porogenic mixture of dodecanol and cyclohexanol, followed by ammonia solution treatment, glutaraldehyde activa...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200500234
更新日期:2006-01-01 00:00:00
abstract::Aberrant telomerase reactivation in differentiated cells represents a major event in oncogenic transformation. Recurrent somatic mutations in the human telomerase reverse transcriptase (TERT) promoter region, predominantly localized to two nucleotide positions, are highly prevalent in many cancer types. Both mutations...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201500327
更新日期:2016-02-01 00:00:00
abstract::As an initial screen for novel markers of renal cancer and to minimise background heterogeneity, we have compared the within-patient profiles of serum samples from seven patients pre- and post-nephrectomy. Samples were depleted of six of the most abundant proteins using Agilent's multiple affinity removal system (MARS...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200800497
更新日期:2008-12-01 00:00:00
abstract::Sarcopenia, defined as age-associated decline of muscle mass and function, is a risk factor for mortality and disability, and comorbid with several chronic diseases such as type II diabetes and cardiovascular diseases. Clinical trials showed that nutritional supplements had positive effects on muscle mass, but not on ...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.201800411
更新日期:2020-03-01 00:00:00
abstract::Attribution of the most probable functions to proteins identified by proteomics is a significant challenge that requires extensive literature analysis. We have developed a system for automated prediction of implicit and explicit biologically meaningful functions for a proteomics study of the nucleolus. This approach u...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200600693
更新日期:2007-03-01 00:00:00
abstract::The Human Kidney and Urine Proteome Project (HKUPP) was initiated in 2005 to promote proteomics research in the nephrology field, to better understand kidney functions as well as pathogenic mechanisms of kidney diseases, and to define novel biomarkers and therapeutic targets. This project was first approved in 2005 by...
journal_title:Proteomics
pub_type:
doi:10.1002/pmic.200800138
更新日期:2008-06-01 00:00:00
abstract::Fusarium oxysporum is an important plant pathogen that causes severe damage of many economically important crop species. Various microorganisms have been shown to inhibit this soil-borne plant pathogen, including non-pathogenic F. oxysporum strains. In this study, F. oxysporum wild-type (WT) MSA 35, a biocontrol multi...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200900716
更新日期:2010-09-01 00:00:00
abstract::We have combined several key sample preparation steps for the use of a liquid matrix system to provide high analytical sensitivity in automated ultraviolet -- matrix-assisted laser desorption/ionisation -- mass spectrometry (UV-MALDI-MS). This new sample preparation protocol employs a matrix-mixture which is based on ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200400956
更新日期:2005-02-01 00:00:00
abstract::Corynebacteria belong to the high G+C Gram-positive bacteria (Actinobacteria) and are closely related to Mycobacterium and Nocardia species. The best investigated member of this group of almost seventy species is Corynebacterium glutamicum, a soil bacterium isolated in 1957, which is used for the industrial production...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.201000786
更新日期:2011-08-01 00:00:00
abstract::Recently (Cytometry 2003, 56A, 71-80), we reported that direct cell-to-cell contact is required for stimulating proliferation of bystander rat liver cells (WB-F344) cocultured with irradiated cells, and neither functional gap junction intercellular communication nor long-range extracellular factors appear to be involv...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200600948
更新日期:2007-06-01 00:00:00
abstract::Daphnia pulex is a keystone species for aquatic habitats and an ecological/evolution model organism. Although significant progress has been made on characterizing its genome, the D. pulex proteome remains largely uncharacterized partially due to abnormally high protein degradation during homogenization and emphasis on...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201700460
更新日期:2018-08-01 00:00:00
abstract::A vast number of human cell lines are available for cell culture model-based studies, and as such the potential exists for discrepancies in findings due to cell line selection. To investigate this concept, the authors determine the relative protein abundance profiles of a panel of eight diverse, but commonly studied h...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.202000218
更新日期:2021-01-01 00:00:00
abstract::Selected or multiple reaction monitoring is a targeted mass spectrometry method (S/MRM-MS), in which many peptides are simultaneously and consistently analyzed during a single liquid chromatography-mass spectrometry (LC-S/MRM-MS) measurement. These capabilities make S/MRM-MS an attractive method to monitor a consisten...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201100543
更新日期:2012-04-01 00:00:00
abstract::Acute kidney injury (AKI) is one of the more frequent and lethal pathological conditions seen in intensive care units. Currently available treatments are not totally effective but stem cell-based therapies are emerging as promising alternatives, especially the use of mesenchymal stromal cells (MSC), although the signa...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201300084
更新日期:2014-06-01 00:00:00