Abstract:
:The array of biomolecules generated by a functioning ecosystem represents both a potential resource for sustainable harvest and a potential indicator of ecosystem health and function. The cupped leaves of the carnivorous pitcher plant, Sarracenia purpurea, harbor a dynamic food web of aquatic invertebrates in a fully functional miniature ecosystem. The energetic base of this food web consists of insect prey, which is shredded by aquatic invertebrates and decomposed by microbes. Biomolecules and metabolites produced by this food web are actively exchanged with the photosynthesizing plant. In this report, we provide the first proteomic characterization of the sacrophagid fly (Fletcherimyia fletcheri), the pitcher plant mosquito (Wyeomyia smithii), and the pitcher-plant midge (Metriocnemus knabi). These three arthropods act as predators, filter feeders, and shredders at distinct trophic levels within the S. purpurea food web. More than 50 proteins from each species were identified, ten of which were predominantly or uniquely found in one species. Furthermore, 19 peptides unique to one of the three species were identified using an assembled database of 100 metazoan myosin heavy chain orthologs. These molecular signatures may be useful in species monitoring within heterogeneous ecosystem biomass and may also serve as indicators of ecosystem state.
journal_name
Proteomicsjournal_title
Proteomicsauthors
Gotelli NJ,Smith AM,Ellison AM,Ballif BAdoi
10.1002/pmic.201000256subject
Has Abstractpub_date
2011-06-01 00:00:00pages
2354-8issue
11eissn
1615-9853issn
1615-9861journal_volume
11pub_type
杂志文章相关文献
PROTEOMICS文献大全abstract::A low virulent Candida albicans mutant, CNC13, deleted in the Mitogen Activated Protein (MAP) kynase HOG1 was used to immunize BALB/c mice. Hog1p is essential for the oxidative stress and hyperosmolarity responses. Several doses and immunization procedures were employed. The protection capacity of the different sera g...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200300678
更新日期:2004-04-01 00:00:00
abstract::Sandwich ELISA microarrays have great potential for validating disease biomarkers. Each ELISA relies on robust-affinity reagents that retain activity when immobilized on a solid surface or when labeled for detection. Single-chain antibodies (scFv) are affinity reagents that have greater potential for high-throughput p...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200701036
更新日期:2008-06-01 00:00:00
abstract::A method for quantitative proteomic analysis based on the selective isolation of multiply charged peptides (RH peptides) containing arginine and histidine residues is described. Two pools of proteins are digested in tandem with lysyl-endopeptidase and trypsin and the primary amino groups of proteolytic peptides are se...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200500836
更新日期:2006-08-01 00:00:00
abstract::Studying microbial interactions by MALDI mass spectrometry imaging (MSI) directly from growing media is a difficult task if high sensitivity is demanded. We present a quick and robust sample preparation strategy for growing fungi (Trichoderma atroviride, Rhizoctonia solani) on glass slides to establish a miniaturized ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201500510
更新日期:2016-06-01 00:00:00
abstract::As an abundant ROS, hydrogen peroxide (H2 O2 ) plays pivotal roles in plant growth and development. In this work, we conducted for the first time an iTRAQ-based quantitative proteomic analysis of wheat seedling growth under different exogenous H2 O2 treatments. The growth of seedlings and roots was significantly restr...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201300042
更新日期:2013-10-01 00:00:00
abstract::Precise protein quantification is essential in comparative proteomics. Currently, quantification bias is inevitable when using proteotypic peptide-based quantitative proteomics strategy for the differences in peptides measurability. To improve quantification accuracy, we proposed an "empirical rule for linearly correl...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201300032
更新日期:2014-07-01 00:00:00
abstract::A curated set of 81 proven and 44 predicted lipoproteins of Escherichia coli K-12 was defined with the combined use of a literature survey, a variety of predictive tools and human expertise. The well-documented Gram-negative proteome of E. coli K-12 was chosen to assess how the different approaches complement each oth...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200300749
更新日期:2004-06-01 00:00:00
abstract::Depletion of abundant proteins is one of the effective ways to improve detection and identification of low-abundance proteins. Our previous study showed that protamine sulfate precipitation (PSP) method can deplete abundant ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) from leaf proteins and is suitable fo...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201400488
更新日期:2015-05-01 00:00:00
abstract::Oviductal fluid (ODF) proteins modulate and support reproductive processes in the oviduct. In the present study, proteins involved in the biological events that precede fertilization have been identified in the rabbit ODF proteome, isolated from the ampulla and isthmus of the oviduct at different time points within 8 ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201800319
更新日期:2019-03-01 00:00:00
abstract::The endothelium is a single layer of cells lining the inside face of all blood vessels. It constitutes a major metabolic organ which is critically involved in the generation and the regulation of multiple physiological and pathological processes such as coagulation, hemostasis, inflammation, atherosclerosis, angiogene...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200300409
更新日期:2003-05-01 00:00:00
abstract::We previously reported that NSC606985, a new camptothecin analog, induces apoptosis of acute myeloid leukemic cells, which is triggered by proteolytic activation of protein kinase C delta (PKC delta). Here, we performed quantitative proteomic analysis of NSC606985-treated and untreated leukemic U937 cells with two-dim...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200800031
更新日期:2009-04-01 00:00:00
abstract::This study was undertaken to identify growth hormone (GH) responsive proteins and protein expression patterns by short-term recombinant human growth hormone (rhGH) therapy in patients with idiopathic short stature (ISS) using proteomic analysis. Seventeen children (14 males and three females) with ISS were included. T...
journal_title:Proteomics
pub_type: 临床试验,杂志文章
doi:10.1002/pmic.201200131
更新日期:2013-04-01 00:00:00
abstract::Many types of cells transit in vitro from a two- to a three-dimensional growth, when they are exposed to microgravity. The underlying mechanisms are not yet understood. Hence, we investigated the impact of microgravity on protein content and growth behavior. For this purpose, the human thyroid cancer cells FTC-133 wer...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201500067
更新日期:2015-09-01 00:00:00
abstract::By mining publicly accessible databases, we have developed a collection of tissue-specific predictive protein expression maps as a function of cancer histological state. Data analysis is applied to the differential expression of gene products in pooled libraries from the normal to the altered state(s). We wish to repo...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200300488
更新日期:2003-08-01 00:00:00
abstract::The aims of the study were to: (i) identify differentially regulated proteins in cerebrospinal fluid (CSF) between multiple sclerosis (MS) patients and non-MS controls; (ii) examine the effect of matching the CSF samples on either total protein amount or volume, and compare four protein normalization strategies for CS...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201500284
更新日期:2016-04-01 00:00:00
abstract::We report on a novel strategy for identification of specific sulfation motifs in chondroitin/dermatan sulfate (CS/DS) chain derived from decorin (Dcn), based on enzyme cleavage and multistage MS (MS(n)). Released CS/DS chains were digested with chondroitin B and in parallel with AC I lyases to obtain oligosaccharides ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200800440
更新日期:2009-07-01 00:00:00
abstract::Knowledge of gender differences is important because nutritional recommendations on the basis of data collected using predominantly male subjects may not be valid for women. In the present study, we performed proteomic analysis in plasma of rats fed a high-fat diet (HFD) using 2-DE combined with MALDI-TOF-MS for analy...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201100127
更新日期:2012-01-01 00:00:00
abstract::The International Protein Index (IPI) database has been one of the most widely used protein databases in MS proteomics approaches. Recently, the closure of IPI in September 2011 was announced. Its recommended replacement is the new UniProt Knowledgebase (UniProtKB) "complete proteome" sets, launched in May 2011. Here,...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201100363
更新日期:2011-11-01 00:00:00
abstract::Seed coat color is an important attribute determining consumption of soybean seeds. Soybean cultivar Mallikong (M) has yellow seed coat while its naturally mutated cultivar Mallikong mutant (MM), has brown colored seed coat. We used integrated proteomics and metabolomics approach to investigate the differences between...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201400453
更新日期:2015-05-01 00:00:00
abstract::Normal Chinese Liver Proteome Expression Profile is one of the major parts of Human Liver Proteome Project. Before starting the studies, it is necessary to examine the interindividual variation of normal liver proteome and evaluate the minimal size of samples for proteomic analysis. In this study, normal liver samples...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200600006
更新日期:2006-10-01 00:00:00
abstract::Human galectin-3 (hGal-3) is a mammalian lectin involved in regulation of RNA splicing, apoptosis, cell differentiation, and proliferation. Multimerized extracellular hGal-3 is thought to crosslink cells by binding to glycoproteins and glycosylated cancer antigens on the cell surface or extracellular matrix. Fluoresce...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200900581
更新日期:2010-05-01 00:00:00
abstract::With recent publications of several large-scale protein-protein interaction (PPI) studies, the realization of the full yeast interaction network is getting closer. Here, we have analysed several yeast protein interaction datasets to understand their strengths and weaknesses. In particular, we investigate the effect of...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200800150
更新日期:2008-11-01 00:00:00
abstract::Automated multidimensional capillary liquid chromatography-tandem mass spectrometry (LC-MS/MS) has been increasingly applied in various large scale proteome profiling efforts. However, comprehensive global proteome analysis remains technically challenging due to issues associated with sample complexity and dynamic ran...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200401055
更新日期:2005-04-01 00:00:00
abstract::The PIK3CA gene encodes for the p110 alpha isoform of PI3 kinase and is one of the most frequently mutated oncogenes in human cancers. However, the mechanisms by which PIK3CA mutations activate cell signaling are not fully understood. Here we used a phosphoproteomic approach to compare differential phosphorylation pat...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201400342
更新日期:2015-01-01 00:00:00
abstract::The influenza virus polymerase is formed by the PB1, PB2 and PA subunits and is required for virus transcription and replication in the nucleus of infected cells. Here we present the characterisation of the complexes formed intracellularly by the influenza polymerase in human cells. The virus polymerase was expressed ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200700508
更新日期:2008-05-01 00:00:00
abstract::Microparticles (MPs) are small membranous particles (100-1000 nm) released under normal steady-state conditions and are thought to provide a communication network between host cells. Previous studies demonstrated that Mycobacterium tuberculosis (M. tb) infection of macrophages increased the release of MPs, and these M...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201400610
更新日期:2015-09-01 00:00:00
abstract::The hair follicle dermal papilla is composed primarily of extracellular matrix (ECM) proteins secreted by resident fibroblasts. Dermal papilla is endowed with hair morphogenic properties, yet its composition is poorly characterized. In an attempt to understand its specificity better, we compared the protein compositio...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200402040
更新日期:2006-11-01 00:00:00
abstract::In order to investigate the unique contribution of individual wine grape (Vitis vinifera) berry tissues and water-deficit to wine quality traits, a survey of tissue-specific differences in protein and selected metabolites was conducted using pericarp (skin and pulp) and seeds of berries from vines grown under well-wat...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200800158
更新日期:2009-05-01 00:00:00
abstract::Surface-enhanced laser desorption/ionization (SELDI) time of flight (TOF) is a mass spectrometry technology for measuring the composition of a sampled protein mixture. A mass spectrum contains peaks corresponding to proteins in the sample. The peak areas are proportional to the measured concentrations of the correspon...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200600181
更新日期:2006-10-01 00:00:00
abstract::Proteins determine the quality of barley in malting and brewing end-uses. In this regard, water-soluble barley proteins play a major role in the formation, stability, and texture of head foams. Our objective was to survey the barley seed proteins that could be involved in the foaming properties of beer. Therefore, two...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200401153
更新日期:2005-07-01 00:00:00