解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::Quantitative acetyl-proteomics, a newly identified post-translational modification, is known to regulate transcriptional activity in different organisms. Neurospora crassa is a model ascomycete fungus maintained for biochemistry and molecular biology research; however, extensive studies of the functions of its acylati...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.202000212
更新日期:2021-01-24 00:00:00
abstract::Biotherapeutics, molecules produced from biological systems, require rigorous purification steps to remove impurities including host cell proteins (HCPs). Regulatory guidelines require manufacturers to monitor process-related impurities along the purification workflow. Mass spectrometry (MS) has recently been consider...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.202000152
更新日期:2021-01-18 00:00:00
abstract::Sporozoites of the malaria parasite Plasmodium are transmitted by mosquitoes and infect the liver for an initial and obligatory round of replication, before exponential multiplication in the blood and onset of the disease. Sporozoites and liver stages provide attractive targets for malaria vaccines and prophylactic dr...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.202000305
更新日期:2021-01-16 00:00:00
abstract::A vast number of human cell lines are available for cell culture model-based studies, and as such the potential exists for discrepancies in findings due to cell line selection. To investigate this concept, the authors determine the relative protein abundance profiles of a panel of eight diverse, but commonly studied h...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.202000218
更新日期:2021-01-01 00:00:00
abstract::Proteins and peptides serve as biomarkers in the context of multiple pathologies. The hypothesis that protein or peptide biomarkers may also be of value in the context of the Covid-19 pandemic appears self-evident. Proteome based biomarkers are not expected to display significant added value in the detection of viral ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.202000229
更新日期:2020-12-01 00:00:00
abstract::The authors present pathwayPCA, an R/Bioconductor package for integrative pathway analysis that utilizes modern statistical methodology, including supervised and adaptive, elastic-net, sparse principal component analysis. pathwayPCA can be applied to continuous, binary, and survival outcomes in studies with multiple c...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201900409
更新日期:2020-11-01 00:00:00
abstract::Innovation in genomics, transcriptomics, and proteomics research has created a plethora of state-of-the-art techniques such as nucleic acid sequencing and mass-spectrometry-based proteomics with paramount impact in the life sciences. While current approaches yield quantitative abundance analysis of biomolecules on an ...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.201900368
更新日期:2020-10-08 00:00:00
abstract::Lysine crotonylation (Kcr) is a recently discovered post-translational modification that potentially regulates multiple biological processes. With an objective to expand the available crotonylation datasets, LC-MS/MS is performed using mouse liver samples under normal physiological conditions to obtain in vivo crotony...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.202000049
更新日期:2020-10-01 00:00:00
abstract::SARS-CoV-2 infection results in a mild-to-moderate disease course in most patients, allowing outpatient self-care and quarantine. However, in approx. 10% of cases a two- or three-phasic critical disease course with starting from day 7 to 10 is observed. To facilitate and plan outpatient care, biomarkers prognosing suc...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.202000202
更新日期:2020-09-10 00:00:00
abstract::The problem with cancer tissue is that its intratumoral heterogeneity and its complexity is extremely high as cells possess, depending on their location and function, different mutations, different mRNA expression and the highest intricacy in the protein pattern. Prior to genomic and proteomic analyses, it is therefor...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.202000077
更新日期:2020-09-01 00:00:00
abstract::Omic technologies have enabled the complete readout of the molecular state of a cell at different biological scales. In principle, the combination of multiple omic data types can provide an integrated view of the entire biological system. This integration requires appropriate models in a systems biology approach. Here...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.201900282
更新日期:2020-09-01 00:00:00
abstract::This review provides a brief overview of the development of data-independent acquisition (DIA) mass spectrometry-based proteomics and selected DIA data analysis tools. Various DIA acquisition schemes for proteomics are summarized first including Shotgun-CID, DIA, MSE , PAcIFIC, AIF, SWATH, MSX, SONAR, WiSIM, BoxCar, S...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.201900276
更新日期:2020-09-01 00:00:00
abstract::Cells have a rich inner structure that is commonly explored by microscopy. Classical biochemical methods that break apart the cells and fractionate them along a gradient have now gotten a new lease on life through modern methods of mass spectrometry-based proteomics. Their common principle is to comprehensively measur...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201900330
更新日期:2020-08-03 00:00:00
abstract::Cancer research is striving toward new frontiers of assigning the correct personalized drug(s) to a given patient. However, extensive tumor heterogeneity poses a major obstacle. Tumors of the same type often respond differently to therapy, due to patient-specific molecular aberrations and/or untargeted tumor subpopula...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.201900227
更新日期:2020-07-01 00:00:00
abstract::Quantitative proteomic studies are contributing greatly to the understanding of the spermatozoon through the provision of detailed information on the proteins spermatozoa acquire and shed in the acquisition of fertility. Extracellular vesicles (EVs) are thought to aid in the delivery of proteins to spermatozoa in the ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201900289
更新日期:2020-06-01 00:00:00
abstract::Protein arginine methyltransferase 5 (PRMT5) is a major enzyme responsible for generating monomethyl and symmetric dimethyl arginine in proteins. PRMT5 is essential for cell viability and development, and its overexpression is observed in a variety of cancers. In the present study, it is found that levels of PRMT5 pro...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201900367
更新日期:2020-05-28 00:00:00
abstract::Most information on molecular processes accompanying and driving adipocyte differentiation are derived from rodent models. Here, we provide a comprehensive analysis of combined transcriptomic and proteomic alterations during adipocyte differentiation in Simpson-Golabi-Behmel Syndrome (SGBS) cells. The SGBS cells are a...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201900405
更新日期:2020-05-08 00:00:00
abstract::Sarcopenia, defined as age-associated decline of muscle mass and function, is a risk factor for mortality and disability, and comorbid with several chronic diseases such as type II diabetes and cardiovascular diseases. Clinical trials showed that nutritional supplements had positive effects on muscle mass, but not on ...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.201800411
更新日期:2020-03-01 00:00:00
abstract::Embryo implantation into maternal endometrium is critical for initiation and establishment of pregnancy, requiring developmental synchrony between endometrium and blastocyst. However, factors regulating human endometrial-embryo cross talk and facilitate implantation remain largely unknown. Extracellular vesicles (EVs)...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201800423
更新日期:2019-12-01 00:00:00
abstract::While metastasis is the primary cause of colorectal cancer (CRC) mortality, the molecular mechanisms underpinning it remains elusive. Metastasis is propagated through driver oncogene/suppressor gene mutations, accompanied by passenger mutations and underlying genomic instability. To understand cancer biology, a unifyi...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201900026
更新日期:2019-11-01 00:00:00
abstract::Hepatocellular carcinoma (HCC) is the second cause of cancer-related deaths worldwide. A clearer understanding of the molecular mechanisms underlying tumor growth and invasiveness remains crucial for developing new therapies. Here, the expression of tetraspanins, a family of plasma membrane organizers involved in tumo...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201900025
更新日期:2019-11-01 00:00:00
abstract::The cancer tissue proteome has enormous potential as a source of novel predictive biomarkers in oncology. Progress in the development of mass spectrometry (MS)-based tissue proteomics now presents an opportunity to exploit this by applying the strategies of comprehensive molecular profiling and big-data analytics that...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201900109
更新日期:2019-11-01 00:00:00
abstract::Various types of unwanted and uncontrollable signal variations in MS-based metabolomics and proteomics datasets severely disturb the accuracies of metabolite and protein profiling. Therefore, pooled quality control (QC) samples are often employed in quality management processes, which are indispensable to the success ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201900264
更新日期:2019-10-01 00:00:00
abstract::Cyanobacteria are oxygenic photosynthetic prokaryotes and play a crucial role in the Earth's carbon and nitrogen cycles. The photoautotrophic cyanobacterium Anabaena sp. PCC 7120 has the ability to fix atmospheric nitrogen in heterocysts and produce hydrogen as a byproduct through a nitrogenase. In order to improve hy...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201800332
更新日期:2019-10-01 00:00:00
abstract::Many acute and chronic lung injuries are incurable and rank as the fourth leading cause of death globally. While stem cell treatment for lung injuries is a promising approach, there is growing evidence that the therapeutic efficacy of stem cells originates from secreted extracellular vesicles (EVs). Consequently, EVs ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201800166
更新日期:2019-09-01 00:00:00
abstract::Secretory proteins of Mycobacterium tuberculosis have created more concern, given their dominant immunogenicity and role in pathogenesis. In view of expensive and time-consuming traditional biochemical experiments, an advanced support vector machine model named SecProMTB is constructed in this study and the proteins a...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201900007
更新日期:2019-09-01 00:00:00
abstract::Niemann-Pick disease, type C1 (NPC1) is a fatal, autosomal recessive, neurodegenerative disorder caused by mutations in the NPC1 gene. As a result of the genetic defect, there is accumulation of unesterified cholesterol and sphingolipids in the late endosomal/lysosomal system causing both visceral and neurological def...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201800285
更新日期:2019-09-01 00:00:00
abstract::Neurons present a highly polarized morphology, often displaying a significantly imbalanced distribution of the cytoplasm between the somatic and axonal domains. This imbalance requires cell-specific mechanisms for the maintenance of the axoplasmic mass during development, neuronal homeostasis, and recovery after injur...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.201900054
更新日期:2019-08-01 00:00:00
abstract::Beneficial microbes have a positive impact on the productivity and fitness of the host plant. A better understanding of the biological impacts and underlying mechanisms by which the host derives these benefits will help to address concerns around global food production and security. The recent development of omics-bas...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.201800105
更新日期:2019-08-01 00:00:00
abstract::The gut microbiome has been shown to play a significant role in human healthy and diseased states. The dynamic signaling that occurs between the host and microbiome is critical for the maintenance of host homeostasis. Analyzing the human microbiome with metaproteomics, metabolomics, and integrative multi-omics analyse...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.201800363
更新日期:2019-08-01 00:00:00
abstract::CRISPR-Cas gene editing holds substantial promise in many biomedical disciplines and basic research. Due to the important functional implications of non-histone chromosomal protein HMG-14 (HMGN1) in regulating chromatin structure and tumor immunity, gene knockout of HMGN1 is performed by CRISPR in cancer cells and the...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201800438
更新日期:2019-07-01 00:00:00
abstract::RNAi experiments are ubiquitously used in cell biology and are achieved by transfection of small interfering RNAs (siRNAs) into cells using a transfection reagent. These results in knock-down of proteins of interest, and the phenotypic consequences are then analyzed. It is reported here that two common RNA interferenc...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201800298
更新日期:2019-07-01 00:00:00
abstract::Microglia, as the resident brain immune cells, can exhibit a broad range of activation phenotypes, which have been implicated in a multitude of central nervous system disorders. Current widely studied microglial cell lines are mainly derived from neonatal rodent brain that can limit their relevance to homeostatic func...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201800469
更新日期:2019-06-01 00:00:00
abstract::The lipid droplet (LD) is an organelle with vital functions found in nearly all organisms. LD proteomic research has provided fundamentally important insights into this organelle's functions. The review provides a summary of LD proteomic studies conducted across diverse organisms and cell and tissue types. The accumul...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.201700223
更新日期:2019-05-01 00:00:00
abstract::The rice heterotrimeric G-protein complex, a guanine-nucleotide-dependent on-off switch, mediates vital cellular processes and responses to biotic and abiotic stress. Exchange of bound GDP (resting state) for GTP (active state) is spontaneous in plants including rice and thus there is no need for promoting guanine nuc...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201800385
更新日期:2019-05-01 00:00:00
abstract::Histidine phosphorylation is a reversible post-translational modification that is known to regulate signal transduction in prokaryotes. However, functional studies in eukaryotes have been largely neglected due to the labile nature of N-linked phosphorylated amino acids. In an effort to help elucidate the heretofore hi...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201800471
更新日期:2019-05-01 00:00:00
abstract::Intrinsically disordered proteins (IDPs) are implicated in a range of human diseases, some of which are associated with the ability to bind to lipids. Although the presence of solvent-exposed hydrophobic regions in IDPs should favor their interactions with low-molecular-weight hydrophobic/amphiphilic compounds, this h...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201800098
更新日期:2019-03-01 00:00:00
abstract::Articles assembled in the second part of this Special Issue describe some experimental and computational approaches for the structural and functional characterization of intrinsically disordered proteins. Since these tools represent specialized gear for the focused analysis of various aspects of dark proteome, they ca...
journal_title:Proteomics
pub_type: 社论
doi:10.1002/pmic.201900085
更新日期:2019-03-01 00:00:00
abstract::Sepsis is an extreme host response to infection that leads to loss of organ function and cardiovascular integrity. Mortality from sepsis is on the rise. Despite more than three decades of research and clinical trials, specific diagnostic and therapeutic strategies for sepsis are still absent. The use of LFQ- and TMT-b...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201800389
更新日期:2019-03-01 00:00:00
abstract::Oviductal fluid (ODF) proteins modulate and support reproductive processes in the oviduct. In the present study, proteins involved in the biological events that precede fertilization have been identified in the rabbit ODF proteome, isolated from the ampulla and isthmus of the oviduct at different time points within 8 ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201800319
更新日期:2019-03-01 00:00:00