Evidence of glutathione transferase complexing and signaling in the model nematode Caenorhabditis elegans using a pull-down proteomic assay.

abstract：:The Human Kidney and Urine Proteome Project (HKUPP) was initiated in 2005 to promote proteomics research in the nephrology field, to better understand kidney functions as well as pathogenic mechanisms of kidney diseases, and to define novel biomarkers and therapeutic targets. This project was first approved in 2005 by...

journal_title：Proteomics

authors： Yamamoto T,Langham RG,Ronco P,Knepper MA,Thongboonkerd V

更新日期：2008-06-01 00:00:00

abstract：:Two Chinese bread wheat cultivars, Jinghua 9 and Zhongmai 175, distinct in grain weight and dough quality, were used to study proteome changes in the embryo and endosperm during grain development using a two-dimensional difference gel electrophoresis (2D-DIGE)-based proteomics approach. In total, 138 and 127 different...

journal_title：Proteomics

authors： Cao H,He M,Zhu C,Yuan L,Dong L,Bian Y,Zhang W,Yan Y

更新日期：2016-05-01 00:00:00

abstract：:A vast number of human cell lines are available for cell culture model-based studies, and as such the potential exists for discrepancies in findings due to cell line selection. To investigate this concept, the authors determine the relative protein abundance profiles of a panel of eight diverse, but commonly studied h...

journal_title：Proteomics

authors： Liu X,Gygi SP,Paulo JA

更新日期：2021-01-01 00:00:00

abstract：:Intracellular serine protease-1 (Isp-1) is a major intracellular serine protease of Bacillus subtilis, whose functions still remain largely unknown. Furthermore, physiological substrates are yet to be determined. To identify Isp-1 substrates, we digested extract obtained from an Isp-1 deficient Bacillus mutant with pu...

journal_title：Proteomics

authors： Lee AY,Goo Park S,Kho CW,Young Park S,Cho S,Lee SC,Lee DH,Myung PK,Park BC

更新日期：2004-11-01 00:00:00

abstract：:The community working on model organisms is growing steadily and the number of model organisms for which proteome data are being generated is continuously increasing. To standardize efforts and to make optimal use of proteomics data acquired from model organisms, a new Human Proteome Organisation (HUPO) initiative on ...

journal_title：Proteomics

authors： Jones AM,Aebersold R,Ahrens CH,Apweiler R,Baerenfaller K,Baker M,Bendixen E,Briggs S,Brownridge P,Brunner E,Daube M,Deutsch EW,Grossniklaus U,Heazlewood J,Hengartner MO,Hermjakob H,Jovanovic M,Lawless C,Lochnit G,Ma

更新日期：2012-02-01 00:00:00

abstract：:To gain a better understanding of salt stress responses in plants, we used a proteomic approach to investigate changes in rice (Oryza sativa) root plasma-membrane-associated proteins following treatment with 150 mmol/L NaCl. With or without a 48 h salt treatment, plasma membrane fractions from root tip cells of a salt...

journal_title：Proteomics

authors： Cheng Y,Qi Y,Zhu Q,Chen X,Wang N,Zhao X,Chen H,Cui X,Xu L,Zhang W

更新日期：2009-06-01 00:00:00

abstract：:MS protein identification and quantitation are key proteomic techniques in biological research. Besides identification of proteins, MS is used increasingly to characterize secondary protein modifications. This often requires trimming the analytical strategy to a specific type of modification. Direct analysis of protei...

journal_title：Proteomics

authors： Niggeweg R,Köcher T,Gentzel M,Buscaino A,Taipale M,Akhtar A,Wilm M

更新日期：2006-01-01 00:00:00

abstract：:Due to the lack of precise markers indicative of its occurrence and progression, coronary artery disease (CAD), the most common type of heart diseases, is currently associated with high mortality in the United States. To systemically identify novel protein biomarkers associated with CAD progression for early diagnosis...

journal_title：Proteomics

authors： Jing L,Parker CE,Seo D,Hines MW,Dicheva N,Yu Y,Schwinn D,Ginsburg GS,Chen X

更新日期：2011-07-01 00:00:00

abstract：:In spite of the high sensitivity of silver staining and the wide dynamic range of various fluorescent detection methods, Coomassie Brilliant Blue staining is still the most widely used protein detection technique for proteins separated by polyacrylamide gel electrophoresis. There are several reasons: Low price, Visibl...

journal_title：Proteomics

authors： Westermeier R

更新日期：2006-09-01 00:00:00

abstract：:Citrulline (Cit) actions on muscle metabolism remain unclear. Those latter were investigated using a proteomic approach on Tibialis muscles from male Sprague-Dawley rats. At 23 months of age, rats were either fed ad libitum (AL group) or subjected to dietary restriction for 12 weeks. At the end of the restriction peri...

journal_title：Proteomics

authors： Faure C,Morio B,Chafey P,Le Plénier S,Noirez P,Randrianarison-Huetz V,Cynober L,Aussel C,Moinard C

更新日期：2013-07-01 00:00:00

abstract：:Most of the energy that is introduced into the oceans by photosynthetic primary producers is in the form of organic matter that then sustains the rest of the food web, from micro to macro-organisms. However, it is the interactions between phototrophs and heterotrophs that are vital to maintaining the nutrient balance ...

journal_title：Proteomics

authors： Christie-Oleza JA,Scanlan DJ,Armengaud J

更新日期：2015-10-01 00:00:00

abstract：:Quantitative proteomic studies are contributing greatly to the understanding of the spermatozoon through the provision of detailed information on the proteins spermatozoa acquire and shed in the acquisition of fertility. Extracellular vesicles (EVs) are thought to aid in the delivery of proteins to spermatozoa in the ...

journal_title：Proteomics

authors： Leahy T,Rickard JP,Pini T,Gadella BM,de Graaf SP

更新日期：2020-06-01 00:00:00

abstract：:Targeted mass spectrometry-based proteomics approaches enable the simultaneous and reproducible quantification of multiple protein analytes across numerous conditions in biology and clinical studies. These approaches involve e.g. selected reaction monitoring (SRM) typically conducted on a triple quadrupole mass spectr...

journal_title：Proteomics

authors： Faktor J,Sucha R,Paralova V,Liu Y,Bouchal P

更新日期：2017-03-01 00:00:00

abstract：:The current treatment for glioblastoma includes temozolomide (TMZ) chemotherapy, yet the mechanism of action of TMZ is not thoroughly understood. Here, we investigated the TMZ-induced changes in the proteome of the glioma-derived cell line (U251) by 2D DIGE. We found 95 protein spots to be significantly altered in the...

journal_title：Proteomics

authors： Kumar DM,Patil V,Ramachandran B,Nila MV,Dharmalingam K,Somasundaram K

更新日期：2013-07-01 00:00:00

abstract：:The allergen content of standardized pollen material is crucial for an effective diagnosis and treatment. However, variations in IgE reactivities of allergic patients to different preparations of Phleum pratense pollen have been reported. In order to define and directly compare the allergen composition of pollen prepa...

journal_title：Proteomics

authors： Schmidt H,Gelhaus C,Nebendahl M,Janssen O,Petersen A

更新日期：2010-12-01 00:00:00

abstract：:Many proteomic studies focus on quantitative aspects, using different stable isotope labeling techniques that require specialized software to analyze the generated data. Here we present jTraqX, an easy-to-use tool for processing and visualizing protein quantification data. jTraqX is platform independent and is compati...

journal_title：Proteomics

authors： Muth T,Keller D,Puetz SM,Martens L,Sickmann A,Boehm AM

更新日期：2010-03-01 00:00:00

abstract：:Mammary gland is made up of a branching network of ducts that end in alveoli. Terminally differentiated mammary epithelial cells (MECs) constitute the innermost layer of aveoli. They are milk-secreting cuboidal cells that secrete milk proteins during lactation. Little is known about the expression profile of proteins ...

journal_title：Proteomics

authors： Janjanam J,Jamwal M,Singh S,Kumar S,Panigrahi AK,Hariprasad G,Jena MK,Anand V,Kumar S,Kaushik JK,Dang AK,Mukesh M,Mishra BP,Srinivasan A,Reddy VS,Mohanty AK

更新日期：2013-11-01 00:00:00

abstract：:We present here the first proteomics analysis of uveal melanoma (UM) cells. These cells represent a good model for the identification of polypeptide markers, which could be developed as diagnostic tools. UM is the most common primary intraocular tumour in adults. In contrast to other cancers, the survival rate of pati...

journal_title：Proteomics

authors： Pardo M,García A,Thomas B,Piñeiro A,Akoulitchev A,Dwek RA,Zitzmann N

更新日期：2005-12-01 00:00:00

abstract：:Prevotella intermedia binds and invades a variety of host cells. This binding is most probably mediated through cell surface proteins termed adhesins. To identify proteins binding to the host extracellular matrix (ECM) component, fibronectin, and study the molecular mechanism underlying bacterial colonization, we appl...

journal_title：Proteomics

authors： Yu F,Iyer D,Anaya C,Lewis JP

更新日期：2006-11-01 00:00:00

abstract：:We describe the first phosphoproteome of the model filamentous fungus Aspergillus nidulans. Phosphopeptides were enriched using titanium dioxide, separated using a convenient ultra-long reverse phase gradient, and identified using a "high-high" strategy (high mass accuracy on the parent and fragment ions) with higher-...

journal_title：Proteomics

authors： Ramsubramaniam N,Harris SD,Marten MR

更新日期：2014-11-01 00:00:00

abstract：:Employing classical two-dimensional electrophoresis (2-DE), amino acid sequencing and immunoblot analysis, we examine for the first time the effect of ozone, a highly notorious environmental pollutant, on rice seedling proteins. Drastic visible necrotic damage to leaf by ozone and consequent increase in ascorbate pero...

journal_title：Proteomics

authors： Agrawal GK,Rakwal R,Yonekura M,Kubo A,Saji H

更新日期：2002-08-01 00:00:00

abstract：:In the postgenomic era, many researchers and organizations have been engaged in structural and functional analyses of proteins. As a part of these efforts, searching for small organic compounds that bind specifically to target proteins is quite important. In this study, we have developed a rational strategy for ligand...

journal_title：Proteomics

authors： Yoshitani N,Satou K,Saito K,Suzuki S,Hatanaka H,Seki M,Shinozaki K,Hirota H,Yokoyama S

更新日期：2005-04-01 00:00:00

abstract：:RNAi experiments are ubiquitously used in cell biology and are achieved by transfection of small interfering RNAs (siRNAs) into cells using a transfection reagent. These results in knock-down of proteins of interest, and the phenotypic consequences are then analyzed. It is reported here that two common RNA interferenc...

journal_title：Proteomics

authors： Özbalci C,Storck EM,Eggert US

更新日期：2019-07-01 00:00:00

abstract：:Current knowledge of the physiology and phylogeny of polycyclic aromatic hydrocarbon (PAH) degrading bacteria often relies on laboratory enrichments and isolations. In the present study, in situ microcosms consisting of activated carbon pellets (BACTRAP®s) were loaded with either (13) C-naphthalene or (13) C-fluorene ...

journal_title：Proteomics

authors： Herbst FA,Bahr A,Duarte M,Pieper DH,Richnow HH,von Bergen M,Seifert J,Bombach P

更新日期：2013-10-01 00:00:00

abstract：:Blood plasma and serum are very useful samples for the detection, identification and quantitation of proteins associated with both health and disease. However, analysis of plasma and serum is a challenge because traces of interesting polypeptides and proteins can be dominated by the very high concentration of albumin ...

journal_title：Proteomics

authors： Baussant T,Bougueleret L,Johnson A,Rogers J,Menin L,Hall M,Aberg PM,Rose K

更新日期：2005-03-01 00:00:00

abstract：:The quantitative analysis of signaling networks requires highly sensitive methods for the time-resolved determination of protein phosphorylation. For this reason, we developed a quantitative protein microarray that monitors the activation of multiple signaling pathways in parallel, and at high temporal resolution. A l...

journal_title：Proteomics

authors： Korf U,Derdak S,Tresch A,Henjes F,Schumacher S,Schmidt C,Hahn B,Lehmann WD,Poustka A,Beissbarth T,Klingmüller U

更新日期：2008-11-01 00:00:00

abstract：:The proteins recovered in exhaled breath condensate (EBC) might be used to non-invasively monitor respiratory diseases. However, the range of proteins and their source are still unresolved and contamination by saliva or a similar protein pattern in the nasal and bronchial compartments may make interpretation of the da...

journal_title：Proteomics

authors： Griese M,Noss J,von Bredow C

更新日期：2002-06-01 00:00:00

abstract：:GlycoMod (http://www.expasy.ch/tools/glycomod/) is a software tool designed to find all possible compositions of a glycan structure from its experimentally determined mass. The program can be used to predict the composition of any glycoprotein-derived oligosaccharide comprised of either underivatised, methylated or ac...

journal_title：Proteomics

authors： Cooper CA,Gasteiger E,Packer NH

更新日期：2001-02-01 00:00:00

abstract：:White spot syndrome virus (WSSV) is a pathogen that causes considerable mortality of the farmed shrimp, Penaeus monodon. Candidate 'vaccines', WSSV envelope protein VP28 and formalin-inactivated WSSV, can provide short-lived protection against the virus. In this study, P. monodon was orally intubated with the aforemen...

journal_title：Proteomics

authors： Kulkarni AD,Kiron V,Rombout JH,Brinchmann MF,Fernandes JM,Sudheer NS,Singh BI

更新日期：2014-07-01 00:00:00

abstract：:Bacillus subtilis is a prolific producer of enzymes and biopharmaceuticals. However, the susceptibility of heterologous proteins to degradation by (extracellular) proteases is a major limitation for use of B. subtilis as a protein cell factory. An increase in protein production levels has previously been achieved by u...

journal_title：Proteomics

authors： Westers L,Westers H,Zanen G,Antelmann H,Hecker M,Noone D,Devine KM,van Dijl JM,Quax WJ

更新日期：2008-07-01 00:00:00