Abstract:
:The allergen content of standardized pollen material is crucial for an effective diagnosis and treatment. However, variations in IgE reactivities of allergic patients to different preparations of Phleum pratense pollen have been reported. In order to define and directly compare the allergen composition of pollen preparations provided by different suppliers, a comprehensive proteome analysis of three different timothy grass pollen extracts was performed. More than 140 proteins were annotated comprising the pollen proteome/allergome in a global 2-D map. With regard to the individual pollen preparations, several major differences in the overall protein composition were detected that also affected known Phleum allergens and their isoforms. Importantly, these differences were also reflected at the level of antibody reactivities in 1-D and 2-D immunoblots. As a consequence, it is suggested that the observed differences should be taken into consideration aiming for a standardized diagnosis and therapy of grass pollen allergies as recommended by international medical agencies.
journal_name
Proteomicsjournal_title
Proteomicsauthors
Schmidt H,Gelhaus C,Nebendahl M,Janssen O,Petersen Adoi
10.1002/pmic.201000451subject
Has Abstractpub_date
2010-12-01 00:00:00pages
4352-62issue
24eissn
1615-9853issn
1615-9861journal_volume
10pub_type
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