Abstract:
:Aphids are major insect pests of cereal crops, acting as virus vectors as well as causing direct damage. The responses of wheat to infestation by cereal aphid (Sitobion avenae) were investigated in a proteomic analysis. Approximately, 500 protein spots were reproducibly detected in the extracts from leaves of wheat seedlings after extraction and 2-DE. Sixty-seven spots differed significantly between control and infested plants following 24 h of aphid feeding, with 27 and 11 up-regulated, and 8 and 21 down-regulated, in local or systemic tissues, respectively. After 8 days, 80 protein spots differed significantly between control and aphid treatments with 13 and 18 up-regulated and 27 and 22 down-regulated in local or systemic tissues, respectively. As positive controls, plants were treated with salicylic acid or methyl jasmonate; 81 and 37 differentially expressed protein spots, respectively, were identified for these treatments. Approximately, 50% of differentially expressed protein spots were identified by PMF, revealing that the majority of proteins altered by aphid infestation were involved in metabolic processes and photosynthesis. Other proteins identified were involved in signal transduction, stress and defence, antioxidant activity, regulatory processes, and hormone responses. Responses to aphid attack at the proteome level were broadly similar to basal non-specific defence and stress responses in wheat, with evidence of down-regulation of insect-specific defence mechanisms, in agreement with the observed lack of aphid resistance in commercial wheat lines.
journal_name
Proteomicsjournal_title
Proteomicsauthors
Ferry N,Stavroulakis S,Guan W,Davison GM,Bell HA,Weaver RJ,Down RE,Gatehouse JA,Gatehouse AMdoi
10.1002/pmic.200900801subject
Has Abstractpub_date
2011-05-01 00:00:00pages
1985-2002issue
10eissn
1615-9853issn
1615-9861journal_volume
11pub_type
杂志文章相关文献
PROTEOMICS文献大全abstract::We present here the first proteomics analysis of uveal melanoma (UM) cells. These cells represent a good model for the identification of polypeptide markers, which could be developed as diagnostic tools. UM is the most common primary intraocular tumour in adults. In contrast to other cancers, the survival rate of pati...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200500030
更新日期:2005-12-01 00:00:00
abstract::The hypothalamus is the central regulatory unit that balances a number of body functions including metabolic rate, hunger, and satiety signals. Hypothalamic neurons monitor and respond to alterations of circulating nutrients and hormones that reflect the peripheral energy status. These extracellular signals are integr...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200700248
更新日期:2007-10-01 00:00:00
abstract::We here present the jmzReader library: a collection of Java application programming interfaces (APIs) to parse the most commonly used peak list and XML-based mass spectrometry (MS) data formats: DTA, MS2, MGF, PKL, mzXML, mzData, and mzML (based on the already existing API jmzML). The library is optimized to be used i...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201100578
更新日期:2012-03-01 00:00:00
abstract::Plant secretomics is a newly emerging area of the plant proteomics field. It basically describes the global study of secreted proteins into the extracellular space of plant cell or tissue at any given time and under certain conditions through various secretory mechanisms. A combination of biochemical, proteomics and b...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.200900514
更新日期:2010-02-01 00:00:00
abstract::The ability to obtain the accurate mass of a protein in a complex sample mixture aids in determining its correct in vivo form. This is important when identifying post-translationally modified proteins, protein variants or isoforms. The central technique used to separate proteins, 2-dimensional gel electrophoresis offe...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200300843
更新日期:2004-10-01 00:00:00
abstract::Non-DNA microarrays, such as protein, peptide and small molecule microarrays, can potentially revolutionize the high-throughput screening tools currently used in basic and pharmaceutical research. However, fundamental obstacles remain that limit their rapid and widespread implementation as an alternative bioanalytical...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200401078
更新日期:2005-05-01 00:00:00
abstract::Niemann-Pick disease, type C1 (NPC1) is a fatal, autosomal recessive, neurodegenerative disorder caused by mutations in the NPC1 gene. As a result of the genetic defect, there is accumulation of unesterified cholesterol and sphingolipids in the late endosomal/lysosomal system causing both visceral and neurological def...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201800285
更新日期:2019-09-01 00:00:00
abstract::Targeted mass spectrometry-based proteomics approaches enable the simultaneous and reproducible quantification of multiple protein analytes across numerous conditions in biology and clinical studies. These approaches involve e.g. selected reaction monitoring (SRM) typically conducted on a triple quadrupole mass spectr...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201600323
更新日期:2017-03-01 00:00:00
abstract::Malt derived from barley malting is an essential raw material for beer brewing. In this study, we performed the first dynamic proteome survey during barley malting using a gel-free proteomics approach. This entailed in-solution tryptic digestion of precipitated proteins and analysis of peptides by nanoliquid chromatog...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201800025
更新日期:2018-08-01 00:00:00
abstract::The HUPO Brain Proteome Project (HUPO BPP) held its 12th workshop in Toronto on 26 September 2009 prior to the HUPO VIII World Congress. The principal aim of this project is to obtain a better understanding of neurodiseases and ageing, with the ultimate objective of discovering prognostic and diagnostic biomarkers, in...
journal_title:Proteomics
pub_type:
doi:10.1002/pmic.201090042
更新日期:2010-06-01 00:00:00
abstract::The International Plant Proteomics Organization (INPPO) is a global platform of the plant proteomics community or, more generally, the scientific community that uses proteomics to address plant biology. Organizing an international conference is one of its initiatives to promote plant proteomics by involving and gather...
journal_title:Proteomics
pub_type:
doi:10.1002/pmic.201570084
更新日期:2015-05-01 00:00:00
abstract::This study was undertaken to identify growth hormone (GH) responsive proteins and protein expression patterns by short-term recombinant human growth hormone (rhGH) therapy in patients with idiopathic short stature (ISS) using proteomic analysis. Seventeen children (14 males and three females) with ISS were included. T...
journal_title:Proteomics
pub_type: 临床试验,杂志文章
doi:10.1002/pmic.201200131
更新日期:2013-04-01 00:00:00
abstract::Atopic dermatitis (AD) is a chronic relapsing inflammatory skin disease typically characterized by a distribution of eczematous skin lesions with lichenification, pruritic excoriations, and dry skin with wide varieties of pathophysiologic aspects. Recently, AD was divided into extrinsic and intrinsic forms according t...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200400998
更新日期:2004-11-01 00:00:00
abstract::Geobacter species predominate in aquatic sediments and submerged soils where organic carbon sources are oxidized with the reduction of Fe(III). The natural occurrence of Geobacter in some waste sites suggests this microorganism could be useful for bioremediation if growth and metabolic activity can be regulated. 2-DE ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200600955
更新日期:2007-11-01 00:00:00
abstract::Due to the limited applicability of conventional protein identification methods to the proteomes of organisms with unsequenced genomes, researchers have developed approaches to identify proteins using mass spectrometry and sequence similarity database searches. Both the integration of mass spectrometry with bioinforma...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.200390004
更新日期:2003-01-01 00:00:00
abstract::Ibuprofen is a member of the proprionic acid group of nonsteroidal anti-inflammatory drugs (NSAID), with the S-enantiomer being more active than the R-enantiomer. It has been shown to display protective effects against neuroinflammation, which is linked to the pathogenesis of several neurodegenerative disorders, inclu...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200700556
更新日期:2008-04-01 00:00:00
abstract::Currently, most researches on the properties of interacting proteins, hubs in particular, are mainly based on an aggregated network of binary interactions. Here, by projecting a yeast-aggregated cell cycle network into four phase (G1, S, G2 and M) sub-networks, we explored the properties of the hubs of the aggregated ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200900053
更新日期:2009-10-01 00:00:00
abstract::Sepsis is an extreme host response to infection that leads to loss of organ function and cardiovascular integrity. Mortality from sepsis is on the rise. Despite more than three decades of research and clinical trials, specific diagnostic and therapeutic strategies for sepsis are still absent. The use of LFQ- and TMT-b...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201800389
更新日期:2019-03-01 00:00:00
abstract::The proteomic profile of hypothalamus, a key organ of CNS, is explored here by employing two widely used MS techniques, i.e. HPLC/ESI-ion trap and HPLC/ESI-quadrupole-TOF MS. Strong cation exchange is used for the fractionation of peptides and protein search engine MASCOT is employed for data query. One hundred and th...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201300073
更新日期:2013-08-01 00:00:00
abstract::To date, proteomics approaches have aimed to either identify novel proteins or change in protein expression/modification in various organisms under normal or disease conditions. One major aspect of functional proteomics is to identify protein biological properties in a given context, however, forward proteomics approa...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.200600211
更新日期:2006-10-01 00:00:00
abstract::Cancer research is striving toward new frontiers of assigning the correct personalized drug(s) to a given patient. However, extensive tumor heterogeneity poses a major obstacle. Tumors of the same type often respond differently to therapy, due to patient-specific molecular aberrations and/or untargeted tumor subpopula...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.201900227
更新日期:2020-07-01 00:00:00
abstract::Recently, several studies employed various proteomic approaches to define diabetes-induced changes in renal proteins. However, functional significance of those datasets in diabetic nephropathy remained unclear. We thus performed integrative proteome network analysis of such datasets followed by various targeted functi...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201700151
更新日期:2017-09-01 00:00:00
abstract::We have used MALDI-MS imaging (MALDI-MSI) to monitor the time dependent appearance and loss of signals when tissue slices are brought rapidly to room temperature for short to medium periods of time. Sections from mouse brain were cut in a cryostat microtome, placed on a MALDI target and allowed to warm to room tempera...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200800201
更新日期:2008-09-01 00:00:00
abstract::While metastasis is the primary cause of colorectal cancer (CRC) mortality, the molecular mechanisms underpinning it remains elusive. Metastasis is propagated through driver oncogene/suppressor gene mutations, accompanied by passenger mutations and underlying genomic instability. To understand cancer biology, a unifyi...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201900026
更新日期:2019-11-01 00:00:00
abstract::We bring a "spectrum" of classical data mining and statistical analysis methods to bear on discrimination of two groups of spectra from 24 diseased and 17 normal patients. Our primary goal is to accurately estimate the generalizability of this small dataset. After an aggressive preprocessing step that reduces consider...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200300516
更新日期:2003-09-01 00:00:00
abstract::The gut microbiome has been shown to play a significant role in human healthy and diseased states. The dynamic signaling that occurs between the host and microbiome is critical for the maintenance of host homeostasis. Analyzing the human microbiome with metaproteomics, metabolomics, and integrative multi-omics analyse...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.201800363
更新日期:2019-08-01 00:00:00
abstract::Many acute and chronic lung injuries are incurable and rank as the fourth leading cause of death globally. While stem cell treatment for lung injuries is a promising approach, there is growing evidence that the therapeutic efficacy of stem cells originates from secreted extracellular vesicles (EVs). Consequently, EVs ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201800166
更新日期:2019-09-01 00:00:00
abstract::Lysine crotonylation (Kcr) is a recently discovered post-translational modification that potentially regulates multiple biological processes. With an objective to expand the available crotonylation datasets, LC-MS/MS is performed using mouse liver samples under normal physiological conditions to obtain in vivo crotony...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.202000049
更新日期:2020-10-01 00:00:00
abstract::We report on quality control performed in the context of a large-scale, multi-institutional study of the immune response in blood samples from prostate cancer patients. The measurements were performed by two commercially available techniques/services: protein arrays and an automated bead-based ELISA-like technique on ...
journal_title:Proteomics
pub_type: 杂志文章,多中心研究
doi:10.1002/pmic.201200082
更新日期:2012-09-01 00:00:00
abstract::Systematic detection of low-abundance proteins in human blood that may be putative disease biomarkers is complicated by an extremely wide range of protein abundances. Hence, depletion of major proteins is one potential strategy for enhancing detection sensitivity in serum or plasma. This study compared a recently comm...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200401228
更新日期:2005-08-01 00:00:00