Comparison of targeted proteomics approaches for detecting and quantifying proteins derived from human cancer tissues.

abstract：:Human butyrophilin (BTN) expression in milk fat globule (MFGM) was evaluated using two dimensional electrophoresis (2-DE) as the separation technique, and peptide mass mapping by matrix-assisted laser desorption/ionisation-mass spectrometry (MALDI-MS) as the identification tool. Since milk composition changes througho...

journal_title：Proteomics

authors： Cavaletto M,Giuffrida MG,Fortunato D,Gardano L,Dellavalle G,Napolitano L,Giunta C,Bertino E,Fabris C,Conti A

更新日期：2002-07-01 00:00:00

abstract：:We present MassSieve, a Java-based platform for visualization and parsimony analysis of single and comparative LC-MS/MS database search engine results. The success of mass spectrometric peptide sequence assignment algorithms has led to the need for a tool to merge and evaluate the increasing data set sizes that result...

journal_title：Proteomics

authors： Slotta DJ,McFarland MA,Markey SP

更新日期：2010-08-01 00:00:00

abstract：:The existence of a gamma-glutamyl cycle consisting of intracellular GSH synthesis, extrusion to the apoplastic space and recovery by gamma-glutamyl transferase (GGT)-assisted degradation into its constituent amino acids, has been demonstrated in plants. To address the significance of this cycle in plant cells, we perf...

journal_title：Proteomics

authors： Tolin S,Arrigoni G,Trentin AR,Veljovic-Jovanovic S,Pivato M,Zechman B,Masi A

更新日期：2013-06-01 00:00:00

abstract：:Most cancers consist of heterogeneous populations of cells with substantial differences in tumorigenicity. Cells that possess self-renewal and tumor-initiating properties are often called cancer stem cells (CSCs). Since CSCs underlie tumor recurrence and metastasis and are resistant to current anti-cancer therapies, n...

journal_title：Proteomics

authors： Sawanobori A,Moriwaki K,Takamatsu S,Kamada Y,Miyoshi E

更新日期：2016-12-01 00:00:00

abstract：:Previous studies have found that some first onset schizophrenia patients show signs of impaired insulin signaling. Also, epidemiological studies have shown that periods of suboptimal nutrition including protein deficiencies during pregnancy can lead to increased incidence of metabolic conditions and psychiatric disord...

journal_title：Proteomics

authors： Guest PC,Urday S,Ma D,Stelzhammer V,Harris LW,Amess B,Pietsch S,Oheim C,Ozanne SE,Bahn S

更新日期：2012-12-01 00:00:00

abstract：:Selected reaction monitoring (SRM) - also known as multiple reaction monitoring (MRM) - has emerged as a promising high-throughput targeted protein quantification technology for candidate biomarker verification and systems biology applications. A major bottleneck for current SRM technology, however, is insufficient se...

journal_title：Proteomics

authors： Shi T,Su D,Liu T,Tang K,Camp DG 2nd,Qian WJ,Smith RD

更新日期：2012-04-01 00:00:00

abstract：:Radiation therapy (RT), combined with chemotherapy, is currently the standard adjuvant approach for UICC-stage II and III rectal cancer patients. Individual rectal tumors display wide ranges of radiosensitivity (RS). The aim of the present study was to identify proteins associated with radioresistance (RR), with the f...

journal_title：Proteomics

authors： Allal AS,Kähne T,Reverdin AK,Lippert H,Schlegel W,Reymond MA

更新日期：2004-08-01 00:00:00

abstract：:Legume symbiosis with rhizobia results in the formation of a specialized organ, the root nodule, where atmospheric dinitrogen is reduced to ammonia. In Lotus japonicus (Lotus), several genes involved in nodule development or nodule function have been defined using biochemistry, genetic approaches, and high-throughput ...

journal_title：Proteomics

authors： Dam S,Dyrlund TF,Ussatjuk A,Jochimsen B,Nielsen K,Goffard N,Ventosa M,Lorentzen A,Gupta V,Andersen SU,Enghild JJ,Ronson CW,Roepstorff P,Stougaard J

更新日期：2014-02-01 00:00:00

abstract：:Staphylococcus aureus, a bacterial, food-borne pathogen of humans, can contaminate raw fruits and vegetables. While physical and chemical methods are available to control S. aureus, scientists are searching for inhibitory phytochemicals from plants. One promising compound from pomegranate is punicalagin, a natural ant...

journal_title：Proteomics

authors： Cooper B,Islam N,Xu Y,Beard HS,Garrett WM,Gu G,Nou X

更新日期：2018-05-01 00:00:00

abstract：:Since the identification of approximately 25,000 proteins from the draft human genome assembly in 2001, estimates of the total have oscillated between 30,000 and 70,000. The recently announced genome closure has not generated a consensus gene count despite this being a key parameter for many areas of biology including...

journal_title：Proteomics

authors： Southan C

更新日期：2004-06-01 00:00:00

abstract：:Beneficial microbes have a positive impact on the productivity and fitness of the host plant. A better understanding of the biological impacts and underlying mechanisms by which the host derives these benefits will help to address concerns around global food production and security. The recent development of omics-bas...

journal_title：Proteomics

authors： Khatabi B,Gharechahi J,Ghaffari MR,Liu D,Haynes PA,McKay MJ,Mirzaei M,Salekdeh GH

更新日期：2019-08-01 00:00:00

abstract：:Elite rice hybrids, when compared to their parental lines, exhibit increased yield and other favorable agronomical traits, such as pathogen- and water-stress resistances, which are described as heterosis, and the molecular mechanism of heterosis remains to be elucidated. Since genomic sequences of the paternal (9311) ...

journal_title：Proteomics

authors： Wang W,Meng B,Ge X,Song S,Yang Y,Yu X,Wang L,Hu S,Liu S,Yu J

更新日期：2008-11-01 00:00:00

abstract：:The hair follicle dermal papilla is composed primarily of extracellular matrix (ECM) proteins secreted by resident fibroblasts. Dermal papilla is endowed with hair morphogenic properties, yet its composition is poorly characterized. In an attempt to understand its specificity better, we compared the protein compositio...

journal_title：Proteomics

authors： Pflieger D,Chabane S,Gaillard O,Bernard BA,Ducoroy P,Rossier J,Vinh J

更新日期：2006-11-01 00:00:00

abstract：:Antibody-based proteomics applied to tissue microarray (TMA) technology provides a very efficient means of visualizing and locating antigen expression in large collections of normal and pathological tissue samples. To characterize antigen expression on TMAs, the use of image analysis methods avoids the effects of huma...

journal_title：Proteomics

authors： Decaestecker C,Lopez XM,D'Haene N,Roland I,Guendouz S,Duponchelle C,Berton A,Debeir O,Salmon I

更新日期：2009-10-01 00:00:00

abstract：:Ruminants make large contributions to sustainable agriculture by converting crop by-products into agricultural food. Multi-omics integrative analysis helps to uncover the underlying molecular mechanisms. The liver metabolome-transcriptome interface (LMTI) in dairy cows, including 3938 significant correlations (p < 0.0...

journal_title：Proteomics

authors： Sun HZ,Wang DM,Liu HY,Liu JX

更新日期：2018-08-01 00:00:00

abstract：:Cardiac conduction defects were found in transgenic mice deficient in urea transporter UT-B. To investigate the molecular mechanisms of the conduction defects caused by UT-B deletion, we studied the protein expression profiles of heart tissue (comprising most conduction system) in wild-type versus UT-B null mice at di...

journal_title：Proteomics

authors： Yu H,Meng Y,Wang LS,Jin X,Gao LF,Zhou L,Ji K,Li Y,Zhao LJ,Chen GQ,Zhao XJ,Yang B

更新日期：2009-02-01 00:00:00

abstract：:Heat shock proteins (HSPs) are crucial for the maintenance of cell integrity during normal cellular growth as well as during pathophysiological conditions. While functioning mainly as molecular chaperones, HSPs also appear to be involved in diverse biological activities, such as apoptosis, carcinogenesis, and cytoprot...

journal_title：Proteomics

authors： Yeo M,Park HK,Kim DK,Cho SW,Kim YS,Cho SY,Paik YK,Hahm KB

更新日期：2004-11-01 00:00:00

abstract：:In this study, various proteomics-based methods were utilized to examine the 14-3-3 protein family in Arabidopsis thaliana. A protein extract was prepared from an Arabidopsis hypocotyl suspension culture and analyzed by two-dimensional gel electrophoresis and immunoblotting with a 14-3-3 monoclonal antibody that recog...

journal_title：Proteomics

authors： Fuller B,Stevens SM Jr,Sehnke PC,Ferl RJ

更新日期：2006-05-01 00:00:00

abstract：:Metabolite flux to UDP-GlcNAc and Golgi N-glycan biosynthesis regulates surface residency of glycoprotein receptors and transporters, and thus sensitivities of cells to extracellular cues. Salvage of GlcNAc increases UDP-GlcNAc and branching of N-glycans progressively, but displays an optimum for cell proliferation an...

journal_title：Proteomics

authors： Lau KS,Khan S,Dennis JW

更新日期：2008-08-01 00:00:00

abstract：:As demonstrated in this study, a CIEF-based multidimensional separation platform not only is compatible with the detergent-based membrane protein preparation protocol, but also achieves both the largest yeast membrane proteome coverage and the most comprehensive analysis of the yeast proteome to date. By using a 1% fa...

journal_title：Proteomics

authors： Wang W,Guo T,Song T,Lee CS,Balgley BM

更新日期：2007-04-01 00:00:00

abstract：:Low levels and long term exposure to benzene is associated with hematotoxicity including aplastic anemia, acute myelogenous leukemia, and lymphoma. Current biomonitoring methods such as urinary phenol, S-phenylmercapturic acid, and trans-trans muconic acid were found to be unreliable as analytical methods to detect be...

journal_title：Proteomics

authors： Joo WA,Kang MJ,Son WK,Lee HJ,Lee DY,Lee E,Kim CW

更新日期：2003-12-01 00:00:00

abstract：:During early stages of embryo development, the brain cavity is filled with embryonic cerebrospinal fluid (E-CSF), a complex fluid containing different protein fractions that contributes to the regulation of the survival, proliferation and neurogenesis of the neuroectodermal stem cells. Using 2-DE, protein sequencing a...

journal_title：Proteomics

authors： Parada C,Gato A,Aparicio M,Bueno D

更新日期：2006-01-01 00:00:00

abstract：:Since its discovery more than 50 years ago, post-translational modification (PTM) of proteins via methylation has grown in prominence, its involvement having been recognised in a number of central processes in the cell. Of these, the best characterised is its role in the epigenetic code. However, there is increasing e...

journal_title：Proteomics

authors： Erce MA,Pang CN,Hart-Smith G,Wilkins MR

更新日期：2012-02-01 00:00:00

abstract：:Immuno-precipitation (IP) experiments using MS provide a sensitive and accurate way of characterising protein complexes and their response to regulatory mechanisms. Differences in stoichiometry can be determined as well as the reliable identification of specific binding partners. The quality control of IP and protein ...

journal_title：Proteomics

authors： ten Have S,Boulon S,Ahmad Y,Lamond AI

更新日期：2011-03-01 00:00:00

abstract：:Microparticles (MPs) are small membranous particles (100-1000 nm) released under normal steady-state conditions and are thought to provide a communication network between host cells. Previous studies demonstrated that Mycobacterium tuberculosis (M. tb) infection of macrophages increased the release of MPs, and these M...

journal_title：Proteomics

authors： Hare NJ,Chan B,Chan E,Kaufman KL,Britton WJ,Saunders BM

更新日期：2015-09-01 00:00:00

abstract：:CID has become a routine method for fragmentation of peptides in shotgun proteomics, whereas electron transfer dissociation (ETD) has been described as a preferred method for peptides carrying labile PTMs. Though both of these fragmentation techniques have their obvious advantages, they also have their own drawbacks. ...

journal_title：Proteomics

authors： Kim MS,Zhong J,Kandasamy K,Delanghe B,Pandey A

更新日期：2011-06-01 00:00:00

abstract：:In an effort to identify tumor-associated proteins from plasma of tumor-bearing mice that may be used as diagnostic biomarkers, we developed a strategy that combines a tumor xenotransplantation model in nude mice with comparative proteomic technology. Five human cancer cell lines (SC-M1, HONE-1, CC-M1, OECM1, GBM 8401...

journal_title：Proteomics

authors： Juan HF,Chen JH,Hsu WT,Huang SC,Chen ST,Yi-Chung Lin J,Chang YW,Chiang CY,Wen LL,Chan DC,Liu YC,Chen YJ

更新日期：2004-09-01 00:00:00

abstract：:Proteomic technologies are being used to discover and identify disease-associated biomarkers. The application of these technologies in the search for potential diagnostic/prognostic biomarkers in the serum of patients has been limited by the presence of highly abundant albumin and immunoglobulins that constitute appro...

journal_title：Proteomics

authors： Ahmed N,Barker G,Oliva K,Garfin D,Talmadge K,Georgiou H,Quinn M,Rice G

更新日期：2003-10-01 00:00:00

abstract：:In this work, for the first time, a novel C60-functionalized magnetic silica microsphere (designated C60-f-MS) was synthesized by radical polymerization of C60 molecules on the surface of magnetic silica microspheres. The resulting C60-f-MS microsphere has magnetite core and thin C60 modified silica shell, which endow...

journal_title：Proteomics

authors： Chen H,Qi D,Deng C,Yang P,Zhang X

更新日期：2009-01-01 00:00:00

abstract：:Prevotella intermedia binds and invades a variety of host cells. This binding is most probably mediated through cell surface proteins termed adhesins. To identify proteins binding to the host extracellular matrix (ECM) component, fibronectin, and study the molecular mechanism underlying bacterial colonization, we appl...

journal_title：Proteomics

authors： Yu F,Iyer D,Anaya C,Lewis JP

更新日期：2006-11-01 00:00:00