Microparticles released from Mycobacterium tuberculosis-infected human macrophages contain increased levels of the type I interferon inducible proteins including ISG15.

abstract：:As one of the most important post-translational modifications, the discovery, isolation, and identification of glycoproteins are becoming increasingly important. In this study, a Con A-magnetic particle conjugate-based method was utilized to selectively isolate the glycoproteins and their glycomes from the healthy don...

journal_title：Proteomics

authors： Yang G,Cui T,Wang Y,Sun S,Ma T,Wang T,Chen Q,Li Z

更新日期：2013-05-01 00:00:00

abstract：:The main goal of this study was to analyze, using proteomic techniques, changes in protein expression of acute myeloid leukemia (AML) cells that could give insights into a better early prognosis for tumor pathophysiology. Proteomic analysis of different subtypes of AML cells was carried out using 2-DE and MALDI-TOF PM...

journal_title：Proteomics

authors： López-Pedrera C,Villalba JM,Siendones E,Barbarroja N,Gómez-Díaz C,Rodríguez-Ariza A,Buendía P,Torres A,Velasco F

更新日期：2006-04-01 00:00:00

abstract：:Proteomics assays hold great promise for unraveling molecular events that underlie human diseases. Effective analysis of clinical samples is essential, but this task is considerably complicated by tissue heterogeneity. Laser capture microdissection (LCM) can be used to selectively isolate target cells from their nativ...

journal_title：Proteomics

authors： Umar A,Luider TM,Foekens JA,Pasa-Tolić L

更新日期：2007-01-01 00:00:00

abstract：:Glycan-binding proteins (GBPs) play an important role in cell adhesion, bacterial/viral infection, and cellular signaling pathways. However, little is known about the precision alteration of GBPs referred to pathological changes in hepatic stellate cells (HSCs) during liver fibrosis. Here, the carbohydrate microarrays...

journal_title：Proteomics

authors： Zhong Y,Qin Y,Dang L,Jia L,Zhang Z,Wu H,Cui J,Bian H,Li Z

更新日期：2015-10-01 00:00:00

abstract：:In higher plants many different genes encode Lhcb proteins that belong to a highly conserved protein family. Evolutionary conservation of this genetic redundancy suggests that individual gene products play different roles in light harvesting and photoprotection depending on environmental conditions. We have tested the...

journal_title：Proteomics

authors： Caffarri S,Frigerio S,Olivieri E,Righetti PG,Bassi R

更新日期：2005-02-01 00:00:00

abstract：:We have developed a surface plasmon resonance (SPR)-based protein microarray to study protein-protein interactions in a high-throughput mode. As a model system, triple protein interactions have been explored with human papillomaviral E6 protein, tumor suppressor p53, and ubiquitin ligase E6AP. Human papillomavirus (HP...

journal_title：Proteomics

authors： Ro HS,Koh BH,Jung SO,Park HK,Shin YB,Kim MG,Chung BH

更新日期：2006-04-01 00:00:00

abstract：:Helicobacter pylori is one of the most prevalent human pathogens in the world and is the aetiological agent of gastritis, peptic ulcer disease and gastric malignancies. In addition H. pylori and other novel members of the genus are capable of successfully colonising the bile-rich niche of the upper intestine and are a...

journal_title：Proteomics

authors： Hynes SO,McGuire J,Falt T,Wadström T

更新日期：2003-03-01 00:00:00

abstract：:Myelination of the CNS is performed by oligodendrocytes (OLs), which have been implicated in brain disorders, such as multiple sclerosis and schizophrenia. We have used the human oligodendroglial cell line MO3.13 to establish an OL reference proteome database. Proteins were prefractionationated by SDS-PAGE and after i...

journal_title：Proteomics

authors： Iwata K,Café-Mendes CC,Schmitt A,Steiner J,Manabe T,Matsuzaki H,Falkai P,Turck CW,Martins-de-Souza D

更新日期：2013-12-01 00:00:00

abstract：:Hpn is a small histidine-rich cytoplasmic protein from Helicobacter pylori and has been recognized as a high-risk factor for several cancers including gastric cancer, colorectal cancer, and MALT lymphoma. However, the relationship between Hpn and cancers remains elusive. In this study, we discovered that Hpn protein e...

journal_title：Proteomics

authors： Liu Y,Wang WM,Zou LY,Li L,Feng L,Pan MZ,Lv MY,Cao Y,Wang H,Kung HF,Pang JX,Fu WM,Zhang JF

更新日期：2017-06-01 00:00:00

abstract：:To better understand the underlying molecular basis of leaf development in maize, a reference map of nuclear proteins in basal region of seedling leaf was established using a combination of 2DE and MALDI-TOF-MS. In total, 441 reproducible protein spots in nuclear proteome of maize leaf basal region were detected with ...

journal_title：Proteomics

authors： Guo B,Chen Y,Li C,Wang T,Wang R,Wang B,Hu S,Du X,Xing H,Song X,Yao Y,Sun Q,Ni Z

更新日期：2014-05-01 00:00:00

abstract：:Brucella melitensis is a facultative intracellular bacterial pathogen that causes brucellosis, a zoonotic disease primarily infecting sheep and goats, characterized by undulant fever, arthritic pain and other neurological disorders in humans. A comprehensive proteomic study of strain 16M was conducted to identify and ...

journal_title：Proteomics

authors： Wagner MA,Eschenbrenner M,Horn TA,Kraycer JA,Mujer CV,Hagius S,Elzer P,DelVecchio VG

更新日期：2002-08-01 00:00:00

abstract：:The surface subproteome of Listeria monocytogenes that includes many proteins already known to be involved in virulence and interaction with host cells has been characterized. A new method for the isolation of a defined surface proteome of low complexity has been established based on serial extraction of proteins by d...

journal_title：Proteomics

authors： Schaumburg J,Diekmann O,Hagendorff P,Bergmann S,Rohde M,Hammerschmidt S,Jänsch L,Wehland J,Kärst U

更新日期：2004-10-01 00:00:00

abstract：:Many tumors arising from epithelial tissues produce mucins, which readily come into contact with infiltrating cells in cancer tissues. MUC2 mucins were purified from the conditioned medium of a colorectal cancer cell line, LS180 cells. It is known that in cancer patients, the number of dendritic cells (DCs) is reduced...

journal_title：Proteomics

authors： Ishida A,Ohta M,Toda M,Murata T,Usui T,Akita K,Inoue M,Nakada H

更新日期：2008-08-01 00:00:00

abstract：:The ability to obtain the accurate mass of a protein in a complex sample mixture aids in determining its correct in vivo form. This is important when identifying post-translationally modified proteins, protein variants or isoforms. The central technique used to separate proteins, 2-dimensional gel electrophoresis offe...

journal_title：Proteomics

authors： Fung KY,Askovic S,Basile F,Duncan MW

更新日期：2004-10-01 00:00:00

abstract：:Beneficial microbes have a positive impact on the productivity and fitness of the host plant. A better understanding of the biological impacts and underlying mechanisms by which the host derives these benefits will help to address concerns around global food production and security. The recent development of omics-bas...

journal_title：Proteomics

authors： Khatabi B,Gharechahi J,Ghaffari MR,Liu D,Haynes PA,McKay MJ,Mirzaei M,Salekdeh GH

更新日期：2019-08-01 00:00:00

abstract：:Trichodesmium is a marine filamentous diazotrophic cyanobacterium and an important contributor of "new" nitrogen in the oligotrophic surface waters of the tropical and sub-tropical oceans. It is unique in that it exclusively fixes N(2) at daytime, although it belongs to the non-heterocystous filamentous segment of the...

journal_title：Proteomics

authors： Sandh G,Ran L,Xu L,Sundqvist G,Bulone V,Bergman B

更新日期：2011-02-01 00:00:00

abstract：:Imbalance in protein homeostasis in specific subcellular organelles is alleviated through organelle-specific stress response pathways. As a canonical example of stress activated pathway, accumulation of misfolded proteins in ER activates unfolded protein response (UPR) in almost all eukaryotic organisms. However, very...

journal_title：Proteomics

authors： Maity S,Basak T,Bhat A,Bhasin N,Ghosh A,Chakraborty K,Sengupta S

更新日期：2014-08-01 00:00:00

abstract：:The community working on model organisms is growing steadily and the number of model organisms for which proteome data are being generated is continuously increasing. To standardize efforts and to make optimal use of proteomics data acquired from model organisms, a new Human Proteome Organisation (HUPO) initiative on ...

journal_title：Proteomics

authors： Jones AM,Aebersold R,Ahrens CH,Apweiler R,Baerenfaller K,Baker M,Bendixen E,Briggs S,Brownridge P,Brunner E,Daube M,Deutsch EW,Grossniklaus U,Heazlewood J,Hengartner MO,Hermjakob H,Jovanovic M,Lawless C,Lochnit G,Ma

更新日期：2012-02-01 00:00:00

abstract：:High temperatures during grain filling have been reported to be one of the factors that can affect the dough properties and quality characteristics of wheat. Responses to high temperature have been related to changes in protein composition at both quantitative and qualitative levels. The present study was conducted to...

journal_title：Proteomics

authors： Majoul T,Bancel E,Triboï E,Ben Hamida J,Branlard G

更新日期：2003-02-01 00:00:00

abstract：:The present review deals with the use of combinatorial ligand libraries, composed by hexapeptides, in the capture and concentration of the low-abundance proteome. This method, first reported in 2005, is compared with other current methodologies aimed at exploring the "deep proteome", such as: depletion of high-abundan...

journal_title：Proteomics

authors： Boschetti E,Righetti PG

更新日期：2009-03-01 00:00:00

abstract：:Quantitative proteomic analysis using stable isotope labeling with amino acids in cell culture (SILAC), as metabolic labeling with MS, has been used as an excellent technique to measure relative abundance change in proteins and post-transitional modifications. Since its development in 2002, SILAC has proven to have un...

journal_title：Proteomics

authors： Jang WE,Kim MS

更新日期：2018-03-01 00:00:00

abstract：:Gene expression analyses of embryonic stem cells (ESCs) will help to uncover or further define signaling pathways and molecular mechanisms involved in the maintenance of self-renewal and pluripotency. We employed a 2-DE-based proteomics approach to analyze human ESC line, Royan H5, in undifferentiated cells and differ...

journal_title：Proteomics

authors： Fathi A,Pakzad M,Taei A,Brink TC,Pirhaji L,Ruiz G,Sharif Tabe Bordbar M,Gourabi H,Adjaye J,Baharvand H,Salekdeh GH

更新日期：2009-11-01 00:00:00

abstract：:Histidine phosphorylation is a reversible post-translational modification that is known to regulate signal transduction in prokaryotes. However, functional studies in eukaryotes have been largely neglected due to the labile nature of N-linked phosphorylated amino acids. In an effort to help elucidate the heretofore hi...

journal_title：Proteomics

authors： Gao Y,Lee H,Kwon OK,Cheng Z,Tan M,Kim KT,Lee S

更新日期：2019-05-01 00:00:00

abstract：:Abnormalities of the anterior cingulate cortex have previously been described in schizophrenia, major depressive disorder and bipolar disorder. In this study 2-DE was performed followed by mass spectrometric sequencing to identify disease-specific protein changes within the anterior cingulate cortex in these psychiatr...

journal_title：Proteomics

authors： Beasley CL,Pennington K,Behan A,Wait R,Dunn MJ,Cotter D

更新日期：2006-06-01 00:00:00

abstract：:Total parenteral nutrition (TPN) is provided as the primary nitrogen source to manage patients with intestinal failure who were not able to sustain themselves on enteral feeds. The most common complication of long-term TPN use is hepatitis. A proteomic approach was used to identify proteins that are differentially exp...

journal_title：Proteomics

authors： Tsai JJ,Kuo HC,Lee KF,Tsai TH

更新日期：2015-11-01 00:00:00

abstract：:The 2-D peptide separations employing mixed mode reversed phase anion exchange (MM (RP-AX)) HPLC in the first dimension in conjunction with RP chromatography in the second dimension were developed and utilised for shotgun proteome analysis. Compared with strong cation exchange (SCX) typically employed for shotgun prot...

journal_title：Proteomics

authors： Phillips HL,Williamson JC,van Elburg KA,Snijders AP,Wright PC,Dickman MJ

更新日期：2010-08-01 00:00:00

abstract：:Proteins undergo PTM, which modulates their structure and regulates their function. Estimates of the PTM occurrence vary but it is safe to assume that there is an important gap between what is currently known and what remains to be discovered. The highest throughput and most comprehensive efforts to catalogue protein ...

journal_title：Proteomics

authors： Ahrné E,Müller M,Lisacek F

更新日期：2010-02-01 00:00:00

abstract：:The quality of samples and of pre-analytical steps are crucial in all biological tests, this is dramatically true in proteomics analysis. In renal cell carcinoma preparation for two-dimensional gel electrophoresis the time elapsed between sample collection and treatment, and the heterogeneity of tissues are considered...

journal_title：Proteomics

authors： Sarto C,Valsecchi C,Mocarelli P

更新日期：2002-11-01 00:00:00

abstract：:Mammalian cells secrete two types of extracellular vesicles either constitutively or in a regulated manner: exosomes (50-100 nm in diameter) released from the intracellular compartment and ectosomes (also called microvesicles, 100-1000 nm in diameter) shed directly from the plasma membrane. Extracellular vesicles are ...

journal_title：Proteomics

authors： Choi DS,Kim DK,Kim YK,Gho YS

更新日期：2013-05-01 00:00:00

abstract：:Aphids are major insect pests of cereal crops, acting as virus vectors as well as causing direct damage. The responses of wheat to infestation by cereal aphid (Sitobion avenae) were investigated in a proteomic analysis. Approximately, 500 protein spots were reproducibly detected in the extracts from leaves of wheat se...

journal_title：Proteomics

authors： Ferry N,Stavroulakis S,Guan W,Davison GM,Bell HA,Weaver RJ,Down RE,Gatehouse JA,Gatehouse AM

更新日期：2011-05-01 00:00:00