Abstract:
:The hair follicle dermal papilla is composed primarily of extracellular matrix (ECM) proteins secreted by resident fibroblasts. Dermal papilla is endowed with hair morphogenic properties, yet its composition is poorly characterized. In an attempt to understand its specificity better, we compared the protein composition of ECM secreted by cultured dermal papilla fibroblasts with that of dermal fibroblasts. ECM proteins are generally large, difficult to solubilize, and abundantly post-translationally modified. We thus implemented an original protocol for analyzing them: ECM samples were enzymatically digested directly in the culture flasks and analyzed by LC-MS/MS. Sequencing of proteolytic peptides by MS/MS yielded protein identification. The relative abundance of a given protein in dermal fibroblast versus dermal papilla samples was estimated by comparing proteolytic peptide intensities detected by MS. Using this approach, several matrix proteins were found to be present at markedly different levels in each ECM type; in particular, thrombospondin 1 and fibronectin appeared to be overrepresented in the dermal papilla fibroblast ECM. MS results were supported by Western blot and immunostaining experiments. In addition, peptide intensities were processed in two ways, which proved to favor either the quantification accuracy or the information precision at the sequence level.
journal_name
Proteomicsjournal_title
Proteomicsauthors
Pflieger D,Chabane S,Gaillard O,Bernard BA,Ducoroy P,Rossier J,Vinh Jdoi
10.1002/pmic.200402040subject
Has Abstractpub_date
2006-11-01 00:00:00pages
5868-79issue
21eissn
1615-9853issn
1615-9861journal_volume
6pub_type
杂志文章相关文献
PROTEOMICS文献大全abstract::Data-independent acquisition (DIA) offers several advantages over data-dependent acquisition (DDA) schemes for characterizing complex protein digests analyzed by LC-MS/MS. In contrast to the sequential detection, selection, and analysis of individual ions during DDA, DIA systematically parallelizes the fragmentation o...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.201400323
更新日期:2015-03-01 00:00:00
abstract::This review provides a brief overview of the development of data-independent acquisition (DIA) mass spectrometry-based proteomics and selected DIA data analysis tools. Various DIA acquisition schemes for proteomics are summarized first including Shotgun-CID, DIA, MSE , PAcIFIC, AIF, SWATH, MSX, SONAR, WiSIM, BoxCar, S...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.201900276
更新日期:2020-09-01 00:00:00
abstract::The PIK3CA gene encodes for the p110 alpha isoform of PI3 kinase and is one of the most frequently mutated oncogenes in human cancers. However, the mechanisms by which PIK3CA mutations activate cell signaling are not fully understood. Here we used a phosphoproteomic approach to compare differential phosphorylation pat...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201400342
更新日期:2015-01-01 00:00:00
abstract::2-D analysis of plant proteomes containing thousands of proteins has limited dynamic resolution because only abundant proteins can be detected. Proteomic assessment of the non-abundant proteins within seeds is difficult when 60-80% is storage proteins. Resolution can be improved through sample fractionation using sepa...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200800875
更新日期:2009-06-01 00:00:00
abstract::Ubiquitin-dependent proteolysis is mediated by the proteasome. To understand the structure and function of the human 26S proteasome, we cloned complete ORFs of 32 human proteasome subunits and conducted a yeast two-hybrid analysis of their interactions with each other. We observed that there are 114 interacting-pairs ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200700588
更新日期:2008-02-01 00:00:00
abstract::Multiple-reaction monitoring (MRM) of peptides has been recognized as a promising technology because it is sensitive and robust. Borrowed from stable-isotope dilution (SID) methodologies in the field of small molecules, MRM is now routinely used in proteomics laboratories. While its usefulness validating candidate tar...
journal_title:Proteomics
pub_type: 评论,杂志文章
doi:10.1002/pmic.201500090
更新日期:2015-04-01 00:00:00
abstract::Modification through beta-elimination has proven to be a reliable first step in the approach for enrichment of serine/threonine-phopshorylated (Ser-/Thr) peptides. However, under harsh basic conditions, Ser-/Thr-glycosylated peptides are susceptible to beta-elimination as well. Therefore, we have optimized these condi...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200600373
更新日期:2006-12-01 00:00:00
abstract::Glycan-binding proteins (GBPs) play an important role in cell adhesion, bacterial/viral infection, and cellular signaling pathways. However, little is known about the precision alteration of GBPs referred to pathological changes in hepatic stellate cells (HSCs) during liver fibrosis. Here, the carbohydrate microarrays...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201500030
更新日期:2015-10-01 00:00:00
abstract::Green tea polyphenols exhibit multiple antitumor activities, and the mechanisms of action are not completely understood. Previously, we reported that green tea extract (GTE)-induced actin remolding is associated with increased cell adhesion and decreased motility in A549 lung cancer cells. To identify the cellular tar...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200800019
更新日期:2009-02-01 00:00:00
abstract::Protein ubiquitination is a powerful modulator of cellular functions. Classically linked to the degradation of proteins, it also plays a role in intracellular localization, DNA damage response, vesicle fusion events, and the immune and transcriptional responses. Ubiquitin is versatile and can code for several distinct...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.201500290
更新日期:2016-01-01 00:00:00
abstract::Rice is susceptible to cold stress and with a future of climatic instability we will be unable to produce enough rice to satisfy increasing demand. A thorough understanding of the molecular responses to thermal stress is imperative for engineering cultivars, which have greater resistance to low temperature stress. In ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201000727
更新日期:2011-05-01 00:00:00
abstract::Human embryonic stem cells (hESCs) have received considerable attention due to their therapeutic potential and usefulness in understanding early development and cell fate commitment. In order to appreciate the unique properties of these pluripotent, self-renewing cells, we have performed an in-depth multidimensional f...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201400132
更新日期:2015-01-01 00:00:00
abstract::Toxoplasma gondii infects a wide range of hosts worldwide, including humans and domesticated animals causing toxoplasmosis disease. Recently, exosomes, small extracellular vesicles (EV) that contain nucleic acids, proteins, and lipids derived from their original cells were linked with disease protection. The effect of...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201600477
更新日期:2017-08-01 00:00:00
abstract::The von Hippel-Lindau (VHL) tumour suppressor gene plays a central role in development of clear cell renal cell carcinoma (RCC). Using a cell line pair generated from the VHL-defective RCC cell line UMRC2 by transfection with vector control or VHL (-/+VHL) and stable isotope labelling with amino acids in cell culture ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200800756
更新日期:2009-04-01 00:00:00
abstract::Sequencing of the human genome opened the way to the exploration of the proteome and this has lead to the identification of large numbers of proteins in complex biological samples. The identification of diagnostic patterns in samples taken from patients to aid diagnosis is in the early stages of development. The solut...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.200700665
更新日期:2008-02-01 00:00:00
abstract::In this study, a method, based on dual column affinity chromatography hyphenated to isotope dilution inductively coupled plasma-quadrupole MS, was developed for selenium determination in selenoprotein P, glutathione peroxidase, and selenoalbumin in human serum samples from a group of healthy volunteers (n=399). Method...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200900677
更新日期:2010-10-01 00:00:00
abstract::FindPept (http://www.expasy.org/tools/findpept.html) is a software tool designed to identify the origin of peptide masses obtained by peptide mass fingerprinting which are not matched by existing protein identification tools. It identifies masses resulting from unspecific proteolytic cleavage, missed cleavage, proteas...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/1615-9861(200210)2:10<1435::AID-PROT1435>3
更新日期:2002-10-01 00:00:00
abstract::The presence of high-abundance proteins in complex protein mixtures often masks low-abundance proteins and causes loss of resolution of 2DE. Protein fractionation steps conducted prior to 2DE can enhance the detection of low-abundance proteins and improve the resolution of 2DE. Here, we report a method to prefractiona...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201100475
更新日期:2012-04-01 00:00:00
abstract::In this study, we performed the first high-throughput and comprehensive proteomic profiling of the rat hippocampal proteome. Using a combination of 2-D LC-MS and data analysis with the Rosetta Elucidator(®) system, we identified 1340 unique proteins. Functional classification showed that most of these were associated ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201000525
更新日期:2011-02-01 00:00:00
abstract::The skin provides protection against environmental stress. However, intrinsic and extrinsic aging causes significant alteration to skin structure and components, which subsequently impairs molecular characteristics and biochemical processes. Here, we have conducted an immunohistological investigation and established t...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201600141
更新日期:2016-10-01 00:00:00
abstract::We describe the first phosphoproteome of the model filamentous fungus Aspergillus nidulans. Phosphopeptides were enriched using titanium dioxide, separated using a convenient ultra-long reverse phase gradient, and identified using a "high-high" strategy (high mass accuracy on the parent and fragment ions) with higher-...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201400063
更新日期:2014-11-01 00:00:00
abstract::Recently, we introduced a novel proteomics method employing a metalloendopeptidase with Lys-N specificity to produce proteolytic peptides. Fragmentation spectra generated by electron transfer dissociation, for a large proportion of the Lys-N proteolytic peptides, were found to be dominated by extensive series of c-typ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200900405
更新日期:2010-03-01 00:00:00
abstract::We have used MALDI-MS imaging (MALDI-MSI) to monitor the time dependent appearance and loss of signals when tissue slices are brought rapidly to room temperature for short to medium periods of time. Sections from mouse brain were cut in a cryostat microtome, placed on a MALDI target and allowed to warm to room tempera...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200800201
更新日期:2008-09-01 00:00:00
abstract::CID has become a routine method for fragmentation of peptides in shotgun proteomics, whereas electron transfer dissociation (ETD) has been described as a preferred method for peptides carrying labile PTMs. Though both of these fragmentation techniques have their obvious advantages, they also have their own drawbacks. ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201000547
更新日期:2011-06-01 00:00:00
abstract::In order to discover novel protein markers indicative of disease processes or drug effects, the proteomics technology platform most commonly used consists of high resolution protein separation by two-dimensional electrophoresis (2-DE), mass spectrometric identification of proteins from stained gel spots and a bioinfor...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200390057
更新日期:2003-04-01 00:00:00
abstract::Chemokines (CCs) are small chemoattractant cytokines involved in a wide variety of biological and pathological processes. Released by cells in the milieu, and extracellular matrix and activating signalling cascades upon binding to specific G protein-coupled receptors (GPCRs), they trigger many cellular events. In vari...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200800211
更新日期:2008-11-01 00:00:00
abstract::The Ser/Thr/Tyr phosphoproteome of Bacillus subtilis was analyzed by a 2-D gel-based approach combining Pro-Q Diamond staining and [(33)P]-labeling. In exponentially growing B. subtilis cells 27 proteins could be identified after staining with Pro-Q Diamond and/or [(33)P]-labeling and one additional protein was labele...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200700232
更新日期:2007-10-01 00:00:00
abstract::Recently (Cytometry 2003, 56A, 71-80), we reported that direct cell-to-cell contact is required for stimulating proliferation of bystander rat liver cells (WB-F344) cocultured with irradiated cells, and neither functional gap junction intercellular communication nor long-range extracellular factors appear to be involv...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200600948
更新日期:2007-06-01 00:00:00
abstract::In recent years, a new paradigm for genome annotation has emerged, termed "proteogenomics," that leverages peptide MS to annotate a genome. This is achieved by mapping peptides to a six-frame translation of a genome, including available splice databases, which may suggest refinements to gene models. Using this approac...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201400185
更新日期:2014-12-01 00:00:00
abstract::In order to investigate the unique contribution of individual wine grape (Vitis vinifera) berry tissues and water-deficit to wine quality traits, a survey of tissue-specific differences in protein and selected metabolites was conducted using pericarp (skin and pulp) and seeds of berries from vines grown under well-wat...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200800158
更新日期:2009-05-01 00:00:00