Abstract:
:Ubiquitin-dependent proteolysis is mediated by the proteasome. To understand the structure and function of the human 26S proteasome, we cloned complete ORFs of 32 human proteasome subunits and conducted a yeast two-hybrid analysis of their interactions with each other. We observed that there are 114 interacting-pairs in the human 26S proteasome. About 10% (11/114) of these interacting-pairs was confirmed by the GST-pull down analysis. Among these observed interacting subunits, 58% (66/114) are novel and the rest 42% (48/114) has been reported previously in human or in other species. We observed new interactions between the 19S regulatory particle and the beta-rings of the 20S catalytic particle and therefore proposed a modified model of the 26S proteasome.
journal_name
Proteomicsjournal_title
Proteomicsauthors
Chen C,Huang C,Chen S,Liang J,Lin W,Ke G,Zhang H,Wang B,Huang J,Han Z,Ma L,Huo K,Yang X,Yang P,He F,Tao Tdoi
10.1002/pmic.200700588subject
Has Abstractpub_date
2008-02-01 00:00:00pages
508-20issue
3eissn
1615-9853issn
1615-9861journal_volume
8pub_type
杂志文章相关文献
PROTEOMICS文献大全abstract::Although the importance of the extracellular signal-regulated kinase (ERK) pathway in regulating the transition from G1 to S has been extensively studied, its role during the G2/M transition is less well understood. Previous reports have shown that inhibition of the ERK pathway in mammalian cells delays entry as well ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200600365
更新日期:2006-08-01 00:00:00
abstract::Chinese tarantula, Chilobrachys jingzhao is one of the most venomous spiders in southern China and its venom is a mixture of various compounds with diversified biological activities. The proteome of C. jingzhao venom was analyzed by proteomic techniques. Proteins with molecular weight of over 10 kDa, indicated by gel-...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200600785
更新日期:2007-06-01 00:00:00
abstract::A protein chip has been developed that allows the simultaneous detection of a multitude of different biowarfare agents. The chip was developed for the ArrayTube platform providing a cheap and easy to handle technology solution that combines a microtube-integrated protein chip with the classical procedure of a sandwich...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200500721
更新日期:2006-05-01 00:00:00
abstract::The Human Kidney and Urine Proteome Project (HKUPP) was initiated in 2005 to promote proteomics research in the nephrology field, to better understand kidney functions as well as pathogenic mechanisms of kidney diseases, and to define novel biomarkers and therapeutic targets. This project was first approved in 2005 by...
journal_title:Proteomics
pub_type:
doi:10.1002/pmic.200800138
更新日期:2008-06-01 00:00:00
abstract::We describe a new approach for the characterization of a digested protein complex with quantitative aspects. Accurate masses of tryptic peptides in the digested complex were acquired by nano-liquid chromatography Fourier transform-ion cyclotron resonance mass spectrometry (MS). The conditions of the electrospray ion s...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200300812
更新日期:2004-09-01 00:00:00
abstract::Recombinant human growth hormone (rhGH) is used for the treatment of several disorders. Structural integrity of rhGH is of critical importance for its clinical use and modifications thereof may act as markers in situations such as rhGH doping, as illegal rhGH-abuse in sports is of increasing interest. In the current s...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200500334
更新日期:2006-02-01 00:00:00
abstract::Protein ubiquitination is a powerful modulator of cellular functions. Classically linked to the degradation of proteins, it also plays a role in intracellular localization, DNA damage response, vesicle fusion events, and the immune and transcriptional responses. Ubiquitin is versatile and can code for several distinct...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.201500290
更新日期:2016-01-01 00:00:00
abstract::Data-independent acquisition (DIA) offers several advantages over data-dependent acquisition (DDA) schemes for characterizing complex protein digests analyzed by LC-MS/MS. In contrast to the sequential detection, selection, and analysis of individual ions during DDA, DIA systematically parallelizes the fragmentation o...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.201400323
更新日期:2015-03-01 00:00:00
abstract::High temperatures during grain filling have been reported to be one of the factors that can affect the dough properties and quality characteristics of wheat. Responses to high temperature have been related to changes in protein composition at both quantitative and qualitative levels. The present study was conducted to...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200390026
更新日期:2003-02-01 00:00:00
abstract::We monitored rates of growth, ATP-synthesis, respiration, and death to assess the sensitivity of the model organism Pseudomonas putida KT2440 to methyl tert-butyl ether (MTBE), and its degree of toxicity. The physiological data obtained suggested that the energy conservation system was the most sensitive site. However...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200300477
更新日期:2003-08-01 00:00:00
abstract::Metabolite flux to UDP-GlcNAc and Golgi N-glycan biosynthesis regulates surface residency of glycoprotein receptors and transporters, and thus sensitivities of cells to extracellular cues. Salvage of GlcNAc increases UDP-GlcNAc and branching of N-glycans progressively, but displays an optimum for cell proliferation an...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200800208
更新日期:2008-08-01 00:00:00
abstract::ProDaC (Proteomics Data Collection), a "Coordination Action" within the 6(th) EU framework programme, was created to support the collection, distribution and public availability of data from proteomics experiments. Within the consortium standards are created and maintained enabling an extensive data collection within ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200800732
更新日期:2009-01-01 00:00:00
abstract::Prior to analysis by mass spectrometry, protein samples are often digested. Maximizing the peptide yield from digestion can increase the number of peptides detected and the confidence in protein identification. To determine the optimal conditions for digestion, the Michaelis-Menten kinetic parameters for Promega seque...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200401268
更新日期:2005-06-01 00:00:00
abstract::In this report we introduce a new concept "proteomic trajectory mapping" for the investigation of a complex phenomenon underlying biological transformation and transition. We define proteomic trajectory to be the kinetic trace of protein expression and present a successful proteomic trajectory mapping of complex molec...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200500813
更新日期:2006-06-01 00:00:00
abstract::Smut caused by Sporisorium scitamineum is one of the important diseases of sugarcane with global significance. Despite the intriguing nature of sugarcane, S. scitamineum interaction, several pertinent aspects remain unexplored. This study investigates the proteome level alterations occurring in the meristem of a S. sc...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201500245
更新日期:2016-04-01 00:00:00
abstract::Sirtuins are a family of NAD(+)-dependent protein deacetylases that regulate cellular functions through deacetylation of a wide range of protein targets. Overexpression of Sir2, the first gene discovered in this family, is able to extend the life span in various organisms. The anti-aging effects of human homologues of...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200800738
更新日期:2009-05-01 00:00:00
abstract::Identification of the phosphorylated residues of bacterial Ser/Thr protein kinase (STPK) substrates still represents a challenging task. Herein, we present a new strategy allowing the rapid determination of phosphoacceptors in kinase substrates, essentially based on the dual expression of the kinase with its substrate...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201000316
更新日期:2010-11-01 00:00:00
abstract::In mass spectrometry-based proteomics, protein identification results usually consist of peptide sequences and database-dependent accession identifiers of the matching proteins. Often certain annotations are only available in particular databases that in turn must be queried by a certain identifier. In order to simpli...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200600018
更新日期:2006-08-01 00:00:00
abstract::The glycan structures of the major and more than ten minor populated isoforms of antithrombin (AT) were determined after separation of the isoforms by IEF using IPG strips. The bands excised from the gel were reduced, derivatized by iodoacetamide and submitted to tryptic digestion. The digest was analyzed by RP-HPLC-E...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200401238
更新日期:2005-10-01 00:00:00
abstract::To better understand the mechanism underlying hepatocellular carcinoma (HCC) metastasis and to search for potential markers for HCC prognosis, differential proteome analysis on two HCC cell strains with high and low metastatic potentials, MHCC97-H and MHCC97-L, was conducted using two-dimensional (2-D) gel electrophor...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200300653
更新日期:2004-04-01 00:00:00
abstract::In the postgenomic era, many researchers and organizations have been engaged in structural and functional analyses of proteins. As a part of these efforts, searching for small organic compounds that bind specifically to target proteins is quite important. In this study, we have developed a rational strategy for ligand...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200401032
更新日期:2005-04-01 00:00:00
abstract::Sandwich ELISA microarrays have great potential for validating disease biomarkers. Each ELISA relies on robust-affinity reagents that retain activity when immobilized on a solid surface or when labeled for detection. Single-chain antibodies (scFv) are affinity reagents that have greater potential for high-throughput p...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200701036
更新日期:2008-06-01 00:00:00
abstract::Geobacter sulfurreducens, generally considered to be a strict anaerobe, is a predominant microbe in subsurface environments, where it utilizes available metals as electron acceptors. To better understand the metabolic processes involved in the metal-reduction capability of this microbe, the proteins expressed by cells...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200500137
更新日期:2006-01-01 00:00:00
abstract::In order to investigate the unique contribution of individual wine grape (Vitis vinifera) berry tissues and water-deficit to wine quality traits, a survey of tissue-specific differences in protein and selected metabolites was conducted using pericarp (skin and pulp) and seeds of berries from vines grown under well-wat...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200800158
更新日期:2009-05-01 00:00:00
abstract::Daphnia pulex is a keystone species for aquatic habitats and an ecological/evolution model organism. Although significant progress has been made on characterizing its genome, the D. pulex proteome remains largely uncharacterized partially due to abnormally high protein degradation during homogenization and emphasis on...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201700460
更新日期:2018-08-01 00:00:00
abstract::SugarQb (www.imba.oeaw.ac.at/sugarqb) is a freely available collection of computational tools for the automated identification of intact glycopeptides from high-resolution HCD MS/MS datasets in the Proteome Discoverer environment. We report the migration of SugarQb to the latest and free version of Proteome Discoverer...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201700436
更新日期:2018-07-01 00:00:00
abstract::This paper reports on the findings of the Biomedical Research Institute, as one of the participants in the pilot study of the HUPO Brain Proteome Project. A biopsy and autopsy study sample derived from human brain was distributed among the participants for proteomic analysis. In our laboratory, attention was focused o...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200600080
更新日期:2006-09-01 00:00:00
abstract::Currently, there are few predictive biomarkers in key biomonitoring species, such as oysters, that can detect heavy metal pollution in coastal waterways. Several attributes make oysters superior to other organisms for positive biomonitoring of heavy metal pollution. In particular, they are filter feeders with a high c...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201100417
更新日期:2012-03-01 00:00:00
abstract::The present review attempts to cover a number of methods that have appeared in the last few years for performing quantitative proteome analysis. However, due to the large number of methods described for both electrophoretic and chromatographic approaches, we have limited this review to conventional two-dimensional (2-...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.200401015
更新日期:2005-02-01 00:00:00
abstract::Protein complexes carry out almost the entire signaling and functional processes in the cell. The protein complex complement of a cell, and its network of complex-complex interactions, is referred to here as the complexome. Computational methods to predict protein complexes from proteomics data, resulting in network r...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.201300230
更新日期:2014-01-01 00:00:00