Abstract:
:Modification through beta-elimination has proven to be a reliable first step in the approach for enrichment of serine/threonine-phopshorylated (Ser-/Thr) peptides. However, under harsh basic conditions, Ser-/Thr-glycosylated peptides are susceptible to beta-elimination as well. Therefore, we have optimized these conditions to achieve a beta-elimination that is highly selective for phosphorylated peptides. This is the first report of selective beta-elimination and enrichment of phosphorylated peptides in the presence of glycosylated peptides.
journal_name
Proteomicsjournal_title
Proteomicsauthors
Poot AJ,Ruijter E,Nuijens T,Dirksen EH,Heck AJ,Slijper M,Rijkers DT,Liskamp RMdoi
10.1002/pmic.200600373subject
Has Abstractpub_date
2006-12-01 00:00:00pages
6394-9issue
24eissn
1615-9853issn
1615-9861journal_volume
6pub_type
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